Objective To evaluate the clinical efficacy of total hip arthroplasty (THA) with no femoral shortening oste?otomy for unilateral CroweⅣ developmental dysplasia of hip. Methods From October 2007 to January 2010, 32 patients with CroweⅣdevelopmental dysplasia of hip in one side underwent THA, including 20 females and 12 males, with an average age of 49.4 ± 9.7 years (range, 23-60 years). There were 15 cases as normal and 17 as mild developmental dysplasia of hip in the other side. The THA were performed with requisite soft tissue release and direct leverage using an elevator but with no femoral shorten?ing osteotomy. The patients' satisfaction, Harris hip score, bilateral leg?length discrepancy and pelvic obliquity was used to assess the clinical results. Results All of patient were followed up for 1-6 years, average 4.0 ± 1.5 years. No loosening or failure of component occurred by the end of follow?up. The Harris hip score was improved from preoperative 36.5±10.3 (20-63) to 89.8± 4.9 (80-97), and the excellent and good rate was 100% (excellent 16 cases, good 16 cases). The satisfactory rate was 93.8%(30/32). The leg?length discrepancy of the bilateral sides and the pelvic obliquity was corrected gradually and the gait returns to normal. Nine cases have valgus knee after THA and 4 cases of them felt uncomfortable after long?distance walk. Femoral nerve injury occurred in 2 cases. All of cases recovered after 1 and 3 months respectively. No infection and dislocation oc?curred. Conclusion THA with no femoral shortening osteotomy can achieve good clinical results in patients with unilateral CroweⅣdevelopmental dysplasia of hip. The discrepancy of leg?length will be diminished with the correction for pelvic obliquity.

Objective To assess the functional restoration in patients with developmental dysplasia of the hip (DDH) who underwent hip resurfacing arthroplasty and to determine whether greater abduction angle of the acetabular component is relat?ed to larger diameter of femoral head component. Methods We reviewed 34 DDH cases (9 hips of 8 males and 25 hips of 24 fe?males, mean age 44.6±11.85 years at the time of surgery) on whom we performed hip resurfacing arthroplasty (HRA) from October 2006 to September 2009. The total hip Arthroplasty (THA) group was consisted of 35 DDH cases (8 hips of 8 males and 27 hips of 25 females, mean age 43.7±10.4 years at the time of surgery). All operations were performed by the same doctor over the same peri?od. Assessment of the functional hip scores was conducted by Harris Hip Scores. A radiographic study was also performed to evalu?ate the implants stability and abduction angle of the acetabular components. All the data was analyzed with Kolmogorov?Smirnov method. Results The mean follow?up was 6.2 years in the HRA group. The Harris Hip Score improved from 54.9±13.2 to 97.3± 6.2 after the surgery. The mean abduction angle of the acetabular component was 51.6° ± 5.33°, hip flexion was 127° ± 6.9° and mean diameter of femoral head was 46.5±1.5 mm. The mean follow?up was 5.9 years in the THA group. The Harris Hip Score im?proved from 51.6±19.7 to 95.6±7.9 after the surgery. The mean abduction angle of the acetabular component was 43.9°±4.90°, hip flexion was 117°±4.2°. There was no failure of the prosthesis, peri?prosthetic fracture and infection in either group. There was sig?nificant difference in the abduction angle of the acetabular component (P<0.05) and flexion of the hip between the two groups (P<0.05). Conclusion Patients in the HRA group had a better functional restoration and larger range of motion. Furthermore, a larger diameter of femoral head component could be achieved by placing the acetabular component in a greater abduction angle, which may contribute to a better long?term stability.

With the improvement of system frame and reconstruction methods in fluorescent molecules tomography (FMT), the FMT technology has been widely used as an important experimental tool in biomedical research. It is necessary to get the 3D-surface profile of the experimental object as the boundary constraints of FMT reconstruction algorithms. We proposed a new 3D-surface reconstruction method based on Fourier transform profilometry (FTP) method under the blue-purple light condition. The slice images were reconstructed using proper image processing methods, frequency spectrum analysis and filtering. The results of experiment showed that the method properly reconstructed the 3D-surface of objects and has the mm-level accuracy. Compared to other methods, this one is simple and fast. Besides its well-reconstructed, the proposed method could help monitor the behavior of the object during the experiment to ensure the correspondence of the imaging process. Furthermore, the method chooses blue-purple light section as its light source to avoid the interference towards fluorescence imaging.
Algorithms ; Biophysical Phenomena ; Fluorescence ; Fourier Analysis ; Image Processing, Computer-Assisted ; Imaging, Three-Dimensional ; methods ; Surface Properties ; Tomography ; methods

Objective To observe the effects of Ganmai Yangxin Decoction on the expression glutamate system in hippocampus of rats with depression;To discuss its mechanism for the treatment of depression.Methods Totally 40 SPF grade SD rats were randomly divided into blank group and modeling group,chronic unpredictable mild stress method was used to establish a depression model.Successfully modeling rats were randomly divided into model group,fluoxetine group and Ganmai Yangxin Decoction group,with 8 rats in each group.The rats in Ganmai Yangxin Decoction group were gavaged with 7.67 g/kg decoction,the fluoxetine group was gavaged with 10 mg/kg fluoxetine,and the blank group and model group were gavaged with 10 mL/kg normal saline,for 4 weeks.and modeling continued while administering the medication.Sucrose preference experiments and forced swimming experiments were used to evaluate the depression status of rats,ELISA was used to detect glutamate content in hippocampal tissue,Nissl staining was used to observe the morphology of neurons in hippocampal CA3 region,immunohistochemical staining was used to detect the expression of GFAP protein in hippocampal CA3 region,Western blot was used to detect the expressions of EAAT1,EAAT2,EAAT3,VGLUT1 and VGLUT2 protein in hippocampal tissue,RT-PCR was used to detect the mRNA expressions of EAAT1,EAAT2 and EAAT3 in hippocampal tissue.Results Compared with the blank group,the body mass of the model group rats decreased(P<0.01),sucrose preference rate decreased,forced swimming immobility time increased(P<0.01),and glutamate content in hippocampal tissue increased(P<0.01),the number of neurons in hippocampal CA3 region decreased,with irregular arrangement and structure changed,the number of Nissl bodies decreased,and the positive expression of GFAP in hippocampal CA3 region decreased(P<0.01),the expressions of EAAT1,EAAT2,EAAT3 protein and mRNA,and VGLUT1 protein in hippocampal tissue decreased(P<0.01),while the expression of VGLUT2 protein increased(P<0.05).Compared with the model group,the Ganmai Yangxin Decoction group and the fluoxetine group showed an increase in body mass(P<0.05),an increase in sucrose preference rate,and a reduction in forced swimming immobility time(P<0.01),and the glutamate content in hippocampal tissue decreased(P<0.01),the number of neurons in the hippocampal CA3 region increased,the structure improved,and there were more Nissl bodies,the positive expression of GFAP in hippocampal CA3 region increased(P<0.01),the expressions of EAAT1,EAAT2,EAAT3 protein and mRNA,and VGLUT1 protein in hippocampal tissue increased(P<0.05,P<0.01).Conclusion Ganmai Yangxin Decoction can improve depression-like behavior in rats,and its mechanism of action is related to up-regulating the expression of glutamate transporters,increasing the number of astrocytes,and reducing glutamate content in hippocampus.