Histone acetylation and deacetylation is an important regulatory way of gene expression in eukaryotic cells. As a key regulatory enzyme, histone deacetylase is overexpressed in many malignant tumors, including hepa- tocellular carcinoma. In addition, it has been suggested to be a potential therapeutic target for solid tumors. In this study, we review the classification, mechanisms, as well as the expression and regulation of histone deactylases in hepatocelhlar carcinoma.

0.05).The 83 samples of HBV genotype B all belonged to subtype Ba,and we had not found subtype Bj.CONCLUSIONS The HBV genotypes among the children carriers of hepatitis B virus in Wenzhou mainly are the genotypes C and B.In two subtypes of genotype B mainly is the subtype Ba.

We hypothesized that the concentrated urea and NaCl solutions may opened the blood-brain barrier to the horseradish peroxidase and trypan blue-albumin complex by shrinking barrier cells and opening up spaces between them. The experiments were carried out on 30 healthy, adult rats. Two experimental groups were used. First, intracarotid perfusion of anesthetized rats were prepared by exposing and catheterizing the left common carotid artery. A test solution of 3.4 M, 3.0 Osm urea and 0.87 M, 1.6 Osm NaCl, was perfused manually for 30 sec. in a cranial direction so as to expel the blood from the pial arterioles of the exposed brain. The pressure, which was not measured, varied between what was required which to expel blood from both arterioles and venules. Five milliliters of the test solution usually were used. Horseradish peroxidase and trypan blue was injected intravenously or through the carotid artery after perfusion. Threshold of barrier damage due to the intracarotid substance was defined as the lowest osmotality which produced obvious blue staining of the brain both on surface observation and coronal section. The effect of a substance was defined as reversible if a threshold concentration did not produce blue staining when the dye was injected 30 min following perfusion. Second, we applied a concentrated solution of 3.0 Osm urea and 1.6 Osm NaCl to the pia-arachnoid of the cerebral cortex, to study the barrier to the intravascular horseradish peroxidase and trypan blue-albumin complex. Hyperosmotic solution of 3.0 Osm urea and 1.6 Osm NaCl, either infused into one internal corotid artery or applied topically to the pia-arachnoid surface of the brain of rats, results in the opening of endo thelial tight junction through which horseradish peroxidase passes from blood to the basal menbrane and astrocytes and neurons. The evidence for this opening of the blood-brain barrier to protein is the entry of peroxidase into the neurons. It was postulated that sufficiently high concentrations of electrolytes or relatively lipid-insoluble non-electrolytes such as urea, osmotically pulled water from the cerebral endothelial cells resulting in their shrinkage. The shrinkage, in turn, was believed to open the tight junction between continuous endothelial cells so that the dyeprotein complex could pass through the junction from blood to neurons. The present study shows that these tight junctions, the sites of the barrier to neuron movement of protein, are indeed opened by the osmotic action of urea or NaCl.

Objective To determine pathogenesis and the suitable time of operation for myelomeningocele associated with hydrocephalus in neonate.Methods 6 underwent head CT scanning, 2 lumbosacral CT scanning and 6 lumbosacral X radiography on 6 patients myelomeningocele complicated with hydrocephalus.Ventriculoperitoneal shunt and repair of the myelomeningocele were performed respectively for one patient.from 1 day to 28 day old.Operation stage 1 in 5 patients.Repair of the myelomeningocele concurred with ventriculoperitoneal shunt. Intracranial pressure was measured in shunting procedure.Results 4 patients had normal intracranial pressure,2 patients increased intracranial pressure in the 6 patients.The volume of the hernial sac had markedly diminished and status of hernial sac had greatly improved wall in the patient who wnderwent two-stage procedures after shunt procedure. Lumbosacral wound healing was good . No recurrent myelomeningocele was found, no hydrocephalus was seen using head CT scanning and clinical manifestation has improved in these patients who were followed up 6 month to 3 year.Conclusions Hydrocephalus may deteriorate the degree of lumbosacral myelomeningocele. Effecacy of vntriculoperitoneal shunt and repair of the myelomeningocele was excellent in myelomeningocele complicated with hydrocephalus in neonate.Micro-operative technique might prevent the occurrence of tethered cord.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Child ; Combined Modality Therapy ; Exercise Therapy ; Female ; Humans ; Low Back Pain ; therapy ; Male ; Middle Aged ; Young Adult

OBJECTIVE:To study inhibitory effects of propofol on PC12 cells injury induced by glutamic acid via mitogen-acti-vated protein kinase/extra-cellular regulated kinase (MAPK/ERK) signal pathway. METHODS:PC12 cells were randomized into normal control group,model group(10 mmol/L glutamic acid),propofol low-concentrations,medium-concentrations and high-con-centrations groups(12.5,25,50 μmol/L+10 mmol/L glutamic acid). The optical density of cells,cell apoptosis,the phosphoryla-tion of ERK1/2 and the expression of c-fos,Bax,Bcl-2 were detected after treated with relevant medicine for 48 h. RESULTS:Compared with normal control group,optical density of cells,the phosphorylation of ERK1/2 and Bcl-2 decreased in model group (P<0.01);apoptotic rate,the expression of c-fos and Bax increased (P<0.01). Compared with model group,optical density of cells,the expression of Bcl-2 and the phosphorylation of ERK1/2 increased in propofol group (P<0.01);apoptosis rate,the ex-pression of c-fos and Bax decreased (P<0.05 or P<0.01). There were statistical significant between the different concentrations (P<0.01). CONCLUSIONS:Propofol can inhibit the apoptosis of PC12 cells induced by glutamic acid,which is associated with the up-regulation of ERK1/2 phosphorylation.

Objective:To understand the related factors of tic disorders(TD) and early intervention among schoolchildren.Methods:Using stratified-cluster random sampling techniques,4,020 children were selected from 61,836 schoolchildren in Daxing district of Beijing.Totally 4,020 children and their parents were asked to fill in a self-designed questionnaire covering both motor and vocal tics and general information.A three-stage procedure was used:screening,interview and clinical investigation.A total of 3,813 children were investigated successfully.The children who were reported with tic symptoms as well as those who were reported by their teachers or classmates were surveyed according to the TD criteria in Diagnostic and Statistical Manual of Mental Disorders-Fourth Edition(DSM-Ⅳ).Eighty-six TD children were investigated respectively about the related factors of TD,and 86 healthy children matched in age,sex and grade were selected as controls.Telephone interview was carried out to find the doubtful TD cases in family members.Those people identified as having tics underwent a semistructured interview to determine whether they had TD according to DSM-Ⅳ criteria.Related risk factors of TD were investigated according to self-designed formulary medical history sheet. Results:Positive TD inheritance family history(7.0%),abdominal delivery style(36.0%),carbonate drink(10.5%),obesity(14.0%) and psychological stress were risk factors of TD(P<0.05).The multivariate analysis of variance indicated that inheritance family history,delivery style,dwelling environment,carbonate drink,parent-child relation,hobby,underachieve,self-care ability were closely related to the high incidence of TD (P<0.05).Conclusion:The occurrence of tic disorders may be related to multiple interacted factors.Early intervention is most important.

Objective To evaluate the left ventricular diastolic dysfunction by tissue Doppler imaging (TDI)in a suprarenal abdominal aortic coarctation model of Lewis rats. Methods Thirty male Lewis rats at age of 8 weeks were used. Myocardial hypertrophy model was obtained by suprarenal abdominal aorta banding with ligation. Mitral annular velocity measurement by TDI and regular two-dimensional echocardiography with M mode were examined at baseline and at 10-,20-,30-and 40-day post banding.Results Compared with baseline,the left ventricular end diastolic and end systolic wall thickness and ventricular dimension were progressively increased,while ejection fraction(EF)and fraction shortening(FS)were decreased(P＜0.0 1).The left ventricle demonstrated centrifugal myocardial hypertrophy.Peak myocardial systolic velocity(Sa)at the mitral annulus did not change significantly throughout this study and there was no significant correlation with EF(r=0.216,P=0.071),and peak myocardial early diastolic (Ea),late diastolic(Aa)at the mitral annulus and Ea/Aa indices also were not stable after aortic banding and there were no significant difference in repeated measures at different time(P＞0.05).But the E/Ea wasincreased and the index had a statistically significant difference(P＜0.001).Conclusions The left ventricleshowed centrifugal myocardial hypertrophy in Lewis rats with abdominal aortic banding.E/Ea radio was a sensitive and stable index to evaluate left ventricular diastolic dysfunction.

Objective To evaluate the efficacy and safety of dorsal root entry zone (DREZ) incision for pain induced by brachial plexus injury. Methods A cohort study was performed in 41 consecutive patients with pain induced by brachial plexus injury treated with DREZ incision from July 2005 to December 2007. Of all, 19 patients were amputated and had phantom limb sensation. Oral pain relief (0%-100%) and complications were followed up by one professional doctor. Oral pain relief rate ≥50% was considered satisfactory outcome. Results The proportion of patients with satisfactory pain relief was 90% (37/41), 81% (30/37), 77% (24/31) and 70% (16/23) at 2 weeks, 3 months, 6 months and last follow-up (over one year) after surgery. At last follow-up, 15 out of 16 patients with over 10 years of pain duration got satisfactory pain relief; while only 16 out of 25 patients with less than 10 years of pain duration got satisfactory pain relief (x2 =4.682, P =0.030). In 16 amputees who were followed up for more than three months, seven patients with alteration of phantom limb sensation got satisfactory pain relief, while only four out of nine patients without alteration of phantom limb sensation got satisfactory pain relief. Among 37 patients followed up for more than three months, severe neurological defects were found in three patients including one with mild motor disturbance in the ipsilateral lower limb and two with severe sensory disturbance in the ipsilateral lower limb. Conclusions DREZ incisions are effective and safe neurosurgical procedure for relieving pain induced by brachial plexus injury. The possible predictive factors of better outcome are the long preoperative pain duration and postoperative phantom limb sensation among amputees.

OBJECTIVE:To probe into the characteristics and the general pattern of adverse drug reactions(ADRs)induced by ritodrine and relevant factors. METHODS:26 literatures according with ADR diagnosis standard were retrieved from CNKI from 1999 to 2009. A total of 337 ADR cases induced by ritodrine were analyzed statistically. RESULTS:The incidence of ritodorine-inducing ADRs was 29.96%(337/1 125). Several clinical manifestations appeared simultaneously. ADR of cardiovascular system accounted for 76.55%(n=284). 5.93% of patients with ADR stop taking medicine because of poor drug tolerance. CONCLUSION: Great importance should be attached to ADR monitoring,adjustment of administration route to guarantee the safety of drug use.