1.Sclerosing Polycystic Adenosis of the Parotid Gland: A Case Report.
Byung Joo JEONG ; Mi Ran KIM ; Zhe Long LIANG ; Bon Seok KOO ; Jin Man KIM
Korean Journal of Pathology 2011;45(Suppl 1):S79-S83
Sclerosing polycystic adenosis (SPA) of the salivary glands is a rare entity analogous to fibrocystic disease of the breast. Less than 50 cases of SPA have been published in the literature. We report the first Korean case of SPA of the right parotid gland. A 34-year-old man presented with a slowly growing right parotid mass. Computed tomography showed a relatively well-demarcated, heterogeneously enhancing mass with multiple small calcifications. Fine needle aspiration showed cohesive sheets of epithelial cells with granular oncocytic cytoplasm and scattered lymphocytes. The parotidectomy specimen showed a 3 cm-sized solid nodular lesion with small cysts. Microscopically, the lesion was an unencapsulated mass of sclerotic fibrous tissue with cystic ducts, multiple calcifications, and lymphoplasma cell infiltration. Sclerosing adenosis and cystic ducts with frequent apocrine-like cells were noted. Familiarity with the cytologic and histological features of SPA is very important making the correct diagnosis.
Adult
;
Biopsy, Fine-Needle
;
Breast
;
Cystic Duct
;
Cytoplasm
;
Epithelial Cells
;
Humans
;
Lymphocytes
;
Parotid Gland
;
Recognition (Psychology)
;
Salivary Glands
2.Sclerosing Polycystic Adenosis of the Parotid Gland: A Case Report.
Byung Joo JEONG ; Mi Ran KIM ; Zhe Long LIANG ; Bon Seok KOO ; Jin Man KIM
Korean Journal of Pathology 2011;45(Suppl 1):S79-S83
Sclerosing polycystic adenosis (SPA) of the salivary glands is a rare entity analogous to fibrocystic disease of the breast. Less than 50 cases of SPA have been published in the literature. We report the first Korean case of SPA of the right parotid gland. A 34-year-old man presented with a slowly growing right parotid mass. Computed tomography showed a relatively well-demarcated, heterogeneously enhancing mass with multiple small calcifications. Fine needle aspiration showed cohesive sheets of epithelial cells with granular oncocytic cytoplasm and scattered lymphocytes. The parotidectomy specimen showed a 3 cm-sized solid nodular lesion with small cysts. Microscopically, the lesion was an unencapsulated mass of sclerotic fibrous tissue with cystic ducts, multiple calcifications, and lymphoplasma cell infiltration. Sclerosing adenosis and cystic ducts with frequent apocrine-like cells were noted. Familiarity with the cytologic and histological features of SPA is very important making the correct diagnosis.
Adult
;
Biopsy, Fine-Needle
;
Breast
;
Cystic Duct
;
Cytoplasm
;
Epithelial Cells
;
Humans
;
Lymphocytes
;
Parotid Gland
;
Recognition (Psychology)
;
Salivary Glands
3.Number changes of circulating endothelial cells in neonatal hypoxemia and its clinical significance
Haifeng LONG ; Xiaoyun LIANG ; Xiaoqiong GU ; Zhe ZHANG ; Yuan ZHANG ; Yuping YIN
International Journal of Laboratory Medicine 2015;(3):294-295
Objective To explore changes in circulating endothelial cells(CECs)numbers in neonatal hypoxemia and its clinical significance.Methods Hladovec method was used to measure the quantity of CECs in 40 cases of neonatus with different degree of hypoxemia(hypoxemia group),and 20 matched healthy newborns were enrolled as control group.Results The numbers of CECs in hypoxemia group were much more than those in control group,and the difference was statistically significant.Meanwhile,there were significant differences in CECs numbers between moderate group and mild group(t =13.43,P <0.05),as well as moderate group and severe group(t=7.698,P <0.05).Conclusion The quantity of circulating ECEs in hypoxic neonatus is correlated with the hy-poxic severity,which may be used as early diagnostic indicator for hypoxemia,and could provide new scientific evidences for early diagnosis,illness judgment and curative effect evaluation.
4.Advance in research on chemical constituents from Notopterygii Rhizoma et Radix.
Shi-ping LI ; Long SHA ; Yi-wu ZHAO ; Zhi-liang XU ; Wen-zhe HUANG ; Zhen-zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2015;40(15):2952-2963
This paper summarized the recent 30 years research progress of the chemical constituents from Notopterygii Rhizoma et Radix. The chemical constituents from Notopterygii Rhizoma et Radix mainly consist of coumarins, polyene-polyacetylenes, sesquiterpenes, phenolic acids, while steroids and flavonoids were less reported. All constituents were confirmed and corrected through SciFinder. We also checked the Chinese name and English name and listed the CAS number of each compound. It can provide some guidelines for the research, development and utilization of Notopterygii Rhizoma et Radix in the future. Whether there is columbianin in the Notopterygii Rhizoma et Radix need to be further researched.
Apiaceae
;
chemistry
;
Drugs, Chinese Herbal
;
analysis
;
Rhizome
;
chemistry
5.The clinical research of the MGMT expression levels in glioma pations.
Hai-cheng XIA ; Zheng-quan ZHU ; Liang LIU ; Hai-long TIAN ; Zhe SUN
Chinese Journal of Experimental and Clinical Virology 2011;25(4):265-267
OBJECTIVETo investigate the correlation between the expression of tumor O (6)-methylquanine DNA methyl-tranferase(MGMT) and pathological grade,and the influence of racial factors on tumor MGMT expression levels for glioma patients.
METHODSCompare and analysis the correlation between the pathological grade and MGMT levels and the racial factors on MGMT expression levels by the immunohistochemical staining on the tumor specimens of 33 Uygur and 61 Han.
RESULTSThe positive rate of 61 Han gliomas pations with MGMT is 45.90% and 33 cases of the Uygur is 30.30% , there's no clear correlation between the racial factors and the tumor MGMT levels. (P >0.05). Comparative the 94 patients with pathological level and tumor MGMT level, there is no clear correlation between pathologic level and MGMT pression in tumor tissues (P >0.05).
CONCLUSIONThere's no clear correlation of tumor MGMT expression and pathological levels; and there's no significant effect between racial factors and expression of glioma MGMT.
Adolescent ; Adult ; Aged ; Child ; China ; ethnology ; DNA Modification Methylases ; genetics ; metabolism ; DNA Repair Enzymes ; genetics ; metabolism ; Female ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Glioma ; enzymology ; ethnology ; genetics ; pathology ; Humans ; Male ; Middle Aged ; Tumor Suppressor Proteins ; genetics ; metabolism ; Young Adult
6.Compare the effect of lipo- and normal prepared Sodium Morrhuate on ECV-304 cell line.
Jun-bo TU ; Hai-long LAN ; Zhuang-qun YANG ; Tie-liang ZHANG ; Yong SONG ; Zhe XING
West China Journal of Stomatology 2006;24(6):491-494
OBJECTIVETo compare the effect of Sodium Morrhuate on ECV-304 between its lipo- and normal preparation.
METHODSThe ECV-304 cell line was supplemented with Sodium Morrhuate and lipo-Sodium Morrhuate in order, and the result on morphology (microscope, Giemsa Staining and electron microscope), cell activity (MTT), and flow cytometer between the two preparation were compared.
RESULTSIn normal preparation group, cell's edema occurred. Chromatin was like catkins. Tumefaction and degeneration of mitochondrion and endoplasmic reticulum appeared. In lipo-Sodium Morrhuate group, the membrane was creased and processus appeared. Chromatin aggregates to the membrane of nucleus was like crescent, and then broken. The apoptotic body was formed. MTT changes showed that the curve of the normal preparation group was steep and the change time was short relatively, which cues the vital cells decreased sharply. The curve of lipo-Sodium Morrhuate group was gentle and the change time was long relatively, which cues the vital cells decreased slowly. The flow cytometer showed that typical apoptosis peak appeared.
CONCLUSIONThe normal preparation group shows an acute toxic effect on ECV-304 cell line, which result in a necrosis course, while lipo-Sodium Morrhuate shows a gradual releasing process, which may indicate a apoptosis course.
Animals ; Apoptosis ; Cell Line ; Humans ; Necrosis ; Sclerosing Solutions ; Sodium Morrhuate
7.Expression profiling of MicroRNAs in hippocampus of rats following traumatic brain injury.
Ting-Yi, SUN ; Xiao-Rui, CHEN ; Zi-Long, LIU ; Li-Li, ZHAO ; Yong-Xiang, JIANG ; Guo-Qiang, QU ; Rong-Shuai, WANG ; Si-Zhe, HUANG ; Liang, LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):548-53
The changes of microRNA expression in rat hippocampus after traumatic brain injury (TBI) were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI: 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regulated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.
8.The experimental study of lipo-sodium morrhuate promoting apoptosis of ECV-304 cells.
Zhuang-qun YANG ; Hai-long LAN ; Jun-bo TU ; Yong SONG ; Tie-liang ZHANG ; Zhe XING
Chinese Journal of Stomatology 2007;42(3):188-189
OBJECTIVETo evaluate the effect of lipo-sodium morrhuate on ECV-304 cell line.
METHODSThe effect lipo-sodium morrhuate was evaluated by toxicology trial (MTT), electron microscope, DNA electrophoresis and flow cytometer.
RESULTSThe toxicology results showed, that the number of vital cells in lipo-sodium morrhuate group decreased slowly. The electron microscope exhibited apoptosis in the lipo-sodium morrhuate group. And there were typical DNA ladder in DNA electrophoresis and typical apoptosis peak in flow cytometer. The apoptosis rate was 22.23%.
CONCLUSIONSUnlike the normal preparation of sodium morrhuate, lipo-sodium morrhuate could induce apoptosis of ECV-304 cell line.
Apoptosis ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Endothelial Cells ; pathology ; Humans ; Liposomes ; Sodium Morrhuate ; pharmacology ; Umbilical Veins ; cytology
9.Adjuvant hormonal therapy immediately after radical surgery for high-risk organ-confined or locally advanced prostate cancer.
Zhao-Long GUAN ; Liang HUANG ; Rui-Zhe ZHAO ; Gong CHENG ; Chao QIN ; Peng-Fei SHAO ; Jie LI ; Li-Xin HUA ; Chang-Jun YIN
National Journal of Andrology 2014;20(12):1093-1097
OBJECTIVETo evaluate the effect of adjuvant hormonal therapy (AHT) immediately after radical surgery for high- risk organ-confined or locally advanced prostate cancer using the PSA-related biochemical relapse rate within 2 years after surgery.
METHODSWe retrospectively analyzed 62 cases of high-risk organ-confined or locally advanced prostate cancer. The patients were treated by laparoscopic radical prostatectomy or radical retropubic prostatectomy after MRI and ECT systemic bone imaging examination, which revealed no regional lymph node or bone metastasis. Thirty-two of the patients (group A) received AHT orally or subcutaneously from 2 weeks to 1 months after operation, and another 30 (group B) were left untreated. We followed up the patients for 2 years, measuring the serum PSA level every 3 months, performing ECT every 6 months, and recording the adverse reactions, medication dura- tion, and the patients'quality of life.
RESULTSAll the operations were successfully accomplished. The rate of 2-year biochemical relapse-free survival was 78.13% (25/32) in group A and 53.33% (16/30) in group B.
CONCLUSIONAHT immediately after radical surgery can improve the rate of biochemical relapse-free survival of the patients with high-risk organ-confined or locally advanced prostate cancer and check the progression and metastasis of the disease.
Aged ; Antineoplastic Agents, Hormonal ; therapeutic use ; Chemotherapy, Adjuvant ; Disease Progression ; Disease-Free Survival ; Humans ; Laparoscopy ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; blood ; Neoplasm Staging ; Prostate-Specific Antigen ; blood ; Prostatectomy ; methods ; Prostatic Neoplasms ; blood ; drug therapy ; pathology ; surgery ; Quality of Life ; Retrospective Studies
10.A case-control study on risk factors of type 2 diabetes mellitus related chronic complications in Baoding city.
Zhe LI ; Shu-qin GUO ; Wen-xuan LIU ; Yun-liang ZHANG ; Lei YANG ; Wen-bin MA ; Dian-wu LIU ; Long-mei TANG
Chinese Journal of Epidemiology 2009;30(12):1292-1296
OBJECTIVETo recognize the main risk factors and to provide evidence for prevention and intervention of type 2 diabetes chronic complications.
METHODSA hospital-based frequency matched case-control study including 200 type 2 diabetes mellitus (T2DM) chronic complications cases and 200 controls without T2DM chronic complications was carried out in Baoding city. Relationships between factors and T2DM chronic complications were analyzed by non-conditional uni-variate and multivariate logistic regression methodologies.
RESULTSHigh C-reactive protein (CRP) (OR = 5.568), dyslipidemia (OR = 4.400), high blood urea nitrogen (BUN) (OR = 4.399), high low density lipoprotein-cholesterol (LDL-C) (OR = 3.594), time of hospitalization (OR = 2.612), grease food intake before developing DM (OR = 2.300), high HbA1c% (OR = 1.747), lack of exercise after the development of DM (OR = 1.672), duration of DM (OR = 1.509), mental stress (OR = 1.427), high-quality sleep (OR = 0.606), well control of blood glucose (OR = 0.517), well control of blood fat (OR = 0.299), insulin injections (OR = 0.155) etc. were all significantly associated with T2DM chronic complications.
CONCLUSIONThe main risk factors of T2DM chronic complications seemed to be related to high CRP, dyslipidemia, high BUN and high LDL-C. The main protective factors were insulin injections, well control of blood fat and blood glucose, good-quality of sleep, while the unique risk factors of cardiovascular disease seemed to be high LDL-C and mental stress. The unique risk factors of neuropathy were lack of exercise after developing DM and the amount of sweet food intake. The duration of DM appeared to be the common risk factor and the common protective factor on those three complications was insulin injection.
Adult ; Aged ; Aged, 80 and over ; Case-Control Studies ; China ; epidemiology ; Diabetes Complications ; epidemiology ; prevention & control ; Diabetes Mellitus, Type 2 ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Risk Factors