OBJECTIVE To search for the sensitive factors of infection by vancomycin-resistant Enterococcus(VRE) as well as the corresponding prevention measures. METHODS Investigation and analysis had been conducted for 15 cases of infection induced by VRE. RESULTS Some of the cases had been infected in communities,but most been infected in hospital,including the old patients in ICU,patients with underlying diseases or respiratory diseases and inpatients staying long in hospital.The infection site was mainly lower respiratory tract,together with the positive results of drug-resistant staphylococci culture. CONCLUSIONS Use of large amount of strong effect broad-spectrum antibiotic as well as use of invasive mechanical ventilation are the risk factors causing infection by VRE.Alternative use of antibiotic of ?-lactamases,amino-glycosides and quinolones can cure the infection,besides,strict isolation and disinfection measures can prevent the infection from spreading in hospital.

This paper introduces a ultra-low frequency modulated low frequency pulse transcutaneous neuromuscalar electrical stimulator.Through clinical practices,it has shown very satisfactory results.

Deconstruction of lignocellulosic plant cell walls to fermentable sugars by biochemical means is impeded by several poorly understood ultrastructural and chemical barriers. Pretreatment is an essential step by altering the morphological and compositional characteristics of biomass to enhance the sugar release during enzymatic hydrolysis. Therefore, getting insight into this field is necessary to improve the conversion of biomass into biofuels. In this review, we highlight our recent understanding on the impact of various promising pretreatments on biomass, with emphasis on the topochemical and ultrastructural changes of plant cell walls that are related to the reduction of recalcitrance and the consequence of saccharification. It will lend support to the scientific research and development with respect to biomass conversion.
Biofuels ; Biomass ; Carbohydrates ; chemistry ; Cell Wall ; ultrastructure ; Fermentation ; Hydrolysis ; Lignin ; chemistry ; Plant Cells ; ultrastructure

Objective To know the inhibitory effects of 5aza-CdR in combination with HDAC inhibitors on RD-ES cells in culture.Methods This study was to investigate that the HDAC inhibitors (MS-275,Trichostatin-A and depsipeptide)and 5azaCdR inhibition effect on RD-ES cells by MTT.High sensitivity DNA kit was used to detect DNA inhibition rate.RT-PCR method was used to detect the expression of ECAD and TSLC1 for 5aza-CdR joint Depsi on RD-ES cells.Results IC50 of 5aza-CdR for RD-ES cell was 40 ng/ml,for 48 h 50 % DNA inhibition rate,the concentration was approximately 50 ng/ml.IC50 of HDAC inhibitors (MS-275,trichostatin-A,Depsi) for RD-ES cell was 400 ng/ml,2.5 ng/ml and 0.6 ng/ml respectively.The inhibition rate of 5aza-CdR jointed HDAC inhibitors was significantly higher compared with the single used groups (P

Objective To discuss the feasibility of diagnosis with needle stereotaction marking by steel wire under mammography and biopsy for nonpalpable early stage breast carcinoma.Methods Eighteen patients with doubtful nidus of breast only in X gram were performed needle stereotaction marking by steel wire under mammography, and removal freemartin biopsies of breast nidus were performed then.Results Localization and biopsy were very successful in all 18 patients. Seven patients were definited to have early stage breast carcinomas and eleven benign diseases.The diagnostic rate for nonpalpable early stage breast carcinoma was 100%.Conclusions Needle stereotaction marking by steel wire undermammography finish off the problem locating accurately breast nidus during operation.It was a credible and perfect technique for diagnoses of nonpalpable early stage breast carcinoma.

11 mm) and presence of central necrosis are the suggestive morphologic features of metastatic paraaortic nodes.

The Montreux definition established in 2017 made it clear that the neonates can not be excluded from the diagnosis of acute respiratory distress syndrome (ARDS), which supported the urge of emphasizing the specificity and importance of neonatal ARDS (nARDS) in the past ten years in China. Neonatal idiopathic respiratory distress syndrome is caused primarily by insufficient pulmonary surfactant system, which pathologically and clinically presented as typical respiratory distress syndrome. While the causes of nARDS often coexisted with underlying conditions, and its pathological and clinical features are a superposition of both respiratory distress syndrome and underlying conditions. Therefore, superimposition is the key to understanding the etiology, pathology, diagnosis, and treatment of nARDS, which is crucial for optimizing the clinical practice of nARDS.

Objective:To investigate the effect of miRNA-1-3p (miR-1-3p) on expression of myocyte enhancer factor 2A (MEF2A) and the biological function of osteosarcoma cells.Methods:The tumor tissues and adjacent normal tissues of 20 patients with osteosarcoma who were clinically diagnosed in Shanxi Provincial Cancer Hospital from January 2019 to January 2020 were collected, and the expression of miR-1-3p in the samples was detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The expression of miR-1-3p in osteosarcoma cell lines U2-OS, SAOS-2, MG63, SW1353 and human normal osteoblast cell line hFOB1.19 was detected by qRT-PCR, then the cell line with the lowest expression of miR-1-3p was selected for follow-up experiments. An overexpression miR-1-3p vector was constructed (miR-1-3p mimcs). The miR-1-3p overexpression group was transfected with miR-1-3p mimcs, and the control group was transfected with empty vector (miR-1-3p nc). CCK-8 method was used to detect the proliferation activity of cells; flow cytometry was used to detect the changes of cell apoptosis and cell cycle. miRwalk database was used to predict the miR-1-3p target gene, and the target gene was verified by dual-luciferase reporter gene assay; Western blot was used to detect the expression of MEF2A protein in cells of each group.Results:Compared with adjacent tissues, the expression of miR-1-3p in osteosarcoma tissues was down-regulated (0.31±0.14 vs. 0.62±0.21), and the difference was statistically significant ( t = 5.31, P<0.01). The expression of miR-1-3p in U2-OS cells was the lowest; compared with the control group, the proliferation activity of U2-OS cells was inhibited in miR-1-3p overexpression group (48 h absorbance value 0.56±0.01 vs. 0.77±0.03, t = 2.77, P<0.01; 72 h absorbance value 0.87±0.02 vs. 1.40±0.03, t = 2.93, P<0.01); G 1/S cell cycle arrest increased [G 1 phase (38.24±0.55)% vs. (32.11±0.80)%, t = 9.27, P = 0.01; S phase (61.24±0.90)% vs. (67.78±0.83)%, t = 7.52, P = 0.02]; early apoptotic rate increased [(11.20±0.12)% vs. (1.50±0.12)%, t = 2.91, P<0.05], miRwalk database predicted that the miR-1-3p target gene was MEF2A. The result of dual-luciferase reporter gene assay showed that miR-1-3p bound to MEF2A 3'UTR, and the luciferase activity of U2-OS cells in miR-1-3p overexpression group was lower than that in the control group (renilla luciferase/firefly luciferase activity ratio 0.53±0.06 vs. 1.00±0.04, t = 4.04, P < 0.05). Western blot showed that the expression of MEF2A protein in U2-OS cells of miR-1-3p overexpression group was lower than that of the control group (protein relative expression 0.41±0.14 vs. 0.77±0.12, t = 3.93, P < 0.05). Conclusions:The low expression of miR-1-3p may be associated with the proliferation, apoptosis and cycle changes of osteosarcoma cells. miR-1-3p can negatively regulate the expression of MEF2A protein and regulate the occurrence and development of osteosarcoma.

Objective:To study the clinical application of laparoscopic combined with multi-video debridement in treatment of complicated infectious pancreatic necrosis (CIPN).Methods:The clinical data of 34 patients with CIPN who were treated at the Department of General Surgery, Xuanwu Hospital, Capital Medical University from August 2017 to December 2019 were retrospectively studied. Based on the different video methods used, these patients were divided into 3 groups: the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group. The number of operations, operation time, blood loss, postoperative complication rates, mortality rates and total length of hospital stay were compared.Results:There were 13 patients in the laparoscopic combined with intraoperative ultrasound group, with age of (56.4±13.4) years. There were 7 patients in the laparoscopic combined with choledochoscopy group, with age of (48.0±8.4) years. There were 14 patients in the laparoscopic group with age of (51.4±15.6) years. The number of operations of the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group were (2.2±1.1), (1.6±0.8), (2.9±1.4), respectively. The number of operations of the laparoscopic combined with choledochoscopy group were significantly less than that of the laparoscopic group ( P<0.05), but there were no significant differences among the other groups ( P>0.05). The operation time of the laparoscopic combined with intraoperative ultrasound group, the laparoscopic combined with choledochoscopy group and the laparoscopic group were (70.5±22.9) min, (65.7±19.9) min, (51.5±15.4) min, respectively. The operation time of the laparoscopic combined with intraoperative ultrasound group was significantly longer than that of the laparoscopic group ( P<0.05), but there were no significant differences among the other groups ( P>0.05). There were no differences in blood loss, postoperative complication rate, mortality rates and total lengths of hospital stay among the three groups ( P>0.05). Conclusion:Laparoscopic combined with multi-video debridement after making full use of the advantages of each of the video methods, can be used to improve treatment outcomes of patients with CIPN.

Constriction, Pathologic ; Coronary Angiography ; Coronary Stenosis ; complications ; Coronary Vessels ; Humans ; Syphilis, Cardiovascular ; complications