Objective:To evaluate the safety and efficacy of domestic intravenous gatifloxacin in the treatment of respiratory tract infections.Methods:A multicenter,double-blind,randomized controlled clinical trials of intravenous gatifloxacin and levofloxacin on patients with respiratory tract infections were conducted.The regimen of the two groups were 0.2g,twice daily,7～14days.Both drugs were given intravenously.Results:In this trial,40 patients were enrolled in gatifloxacin group,39 patients were enrolled in levofloxacin group.The overall efficacy rates and cure rate in trial and control group were 95%,65% and 94.87%,64.1% respectively.The bacterial clearance rates were 86.8% and 81.1% respectively in two groups.The adverse drug reaction rates were 7.5% and 10.2% in trial and control group respectively.There were no statistically significant differences in the above results between the two groups.The activity of gatifloxacin against gram-positive organisms was generally higher than that of the other 4 antibacterial agents.Antibacterial activity of gatifloxacin against gram-negtive bacterium was similar or stronger than the other antibacterial agents.Conclusion:The results suggest that domestic gatifloxacin injection with powerful antibacterial activity and wide spectrum is a highly effective and safe broad-spectrum new quinolone in treating respiratory tract infections.

Objective:To evaluate the safety and efficacy of domestic cefepime in the treatment of lower-respiratory tract bacterial infections.Methods:A double-blind,randomized control clinical trial of intravenous cefepime on patients with lower-respiratory tract bacterial infections were conducted.The regimen of the two groups were 2g,twice daily for 7 to14ds.Both drugs were given intravenously.Results:In this trial,30 patients were enrolled in trial group and other 30 patients were enrolled in control group.The overall efficacy rates and cure rate in trial and control group were 93.33%?63.33% and 96.67%?70% respectively.The bacterial clearance rates were 96% and 100% respectively,and the adverse drug reaction rates were 3.3% and 0%. There was no statistical significant differences in the above results between the two groups.The activity of cefepime against gram-positive organisms was generally higher than that of the other 4 antibacterial agents.Conclusion:The results suggested that demostic cefepime with powerful antibacterial activity and wide spectrum has the same safety and efficacy as imported one in treating lower-respiratory tract bacterial infections.

Objective: To evaluate the safety and efficacy of domestic intravenous doxycycline in the treatment of urinary tract infections.Methods:A multicenter,double-blind,randomized controlled clinical trial of intravenous doxycycline and azithromycin on patients with urinary tract infections were conducted.The dosage of doxycycline was 0.2g,once daily,for 5~12d and that of azithromycin was 0.5g,once daily,for 5~12d.Both drugs were given intravenously.Results: In this trial,62 patients were enrolled in doxycycline group,and 60 were enrolled in azithromycin group.The overall efficacy rates and cure rate in trial and control group were 100%,90.32% and 73.33%,95% respectively.There was statistical significant difference in the cure rates between the two groups.The bacterial clearance rates in two groups were 92.5% and 88.9% respectively.The adverse drug reaction rates were7.9% and6.7% respectively.There were no statistical significant differences in the above results between the two groups.The activity of doxycycline against gram-positive organisms was generally higher than that of the other 5 antibacterial agents.Antibacterial activity of doxycycline against gram-negtive bacterium was similar to or stronger than the other antibacterial agents.Conclusion: The results suggest that domestic doxycycline injection with powerful antibacterial activity and wide spectrum is highly effective in treating urinary tract infections.

The conventional approach of systemic viral gene delivery is often associated with limited therapeutic efficacy and toxicity because of a lack of tumor specificity. Mesenchymal stem cells have the tumortracking abilities which provide possibility that the stable and high level of anti- cancer gene expression could be acquired within local tumor and a novel approach for better tumor-targeted gene delivery.

BACKGROUND:Bmi-1 (B-cel-specific moloney murineleukemia virus insertion site), a colorectal cancer stem cellgene is of great significance in the regulation of telomere reverse transcriptase activity and transcriptional status as wel as cellaging and carcinogenesis.
OBJECTIVE:To investigate the correlation between Bmi-1 expression and clinicopathological changes of colorectal cancer.
METHODS:The expression of Bmi-1 mRNA in carcinoma cells was detected in 50 cases of colorectal cancer using quantitative real-time PCR. The expression of Bmi-1 protein in cancer tissue was detected by hematoxylin-eosin staining and immunohistochemical staining SP method.
RESULTS AND CONCLUSION:Sex, age, and tumor size showed no different effects on the expression of Bmi-1 mRNA. The expression of Bmi-1 protein showed no significant difference in patients who were different in sex, age, tumor size and Duke staging of tumor. The expressions of Bmi-1 mRNA and protein in patients with lymph node metastasis and depth of invasion deeper than the serosa were much higher than those in patients with no lymph node metastasis and depth of invasion shal ower than the serosa (P<0.05). The expression of Bmi-1 mRNA was much higher in patients with higher Duke staging cancer (P<0.05). The expression of Bmi-1 mRNA and protein are closely related to different pathological features of colorectal cancer, such as metastasis, infiltration, and Duke staging.

Objective To investigate the relationship between coronary artery disease, other vascular risk factors and mild cognitive impairment in elderly patients. Methods A total of 196 inpatients were collected from the departments of cardiovascular internal medicine from April to September 2015.The unified questionnaires of cognitive status and standardized survey were developed. The patients were divided into 133 cases of mild cognitive impairment and 63 cases of cognitively normal by Montreal Cognitive Assessment Scale. The patients were divided into 79 cases of coronary heart disease and 117 cases of negative control by coronary angiography. Coronary artery disease and other vascular risk factors for mild cognitive impairment were analyzed. Results The levels of education and proportion of coronary heart disease were (9.23 ± 4.34) years and 45.9%(61/133) in mild cognitive impairment patients, and (12.27 ± 3.79) years and 28.6%(18/63) in cognitively normal patients. There were significant differences between the two(t=4.77, P<0.05,χ2=5.31, P<0.05). Multivariate Logistic regression analysis showed that degree of education was a protective factor for mild cognitive impairment with odds ratio ( OR) 0.86, 95%confidence interval (CI) 0.77 to 0.91 and P=0.00. Coronary heart disease was an independent risk factor for mild cognitive impairment (OR=2.02, 95%CI 1.03 to 4.00; P = 0.04) . The total scores of Montreal Cognitive Assessment Scale and delayed memory were (20.9±4.8), (22.4±5.6) points and (2.1± 1.6), (2.6 ± 1.8) points in coronary heart disease and negative control patients. There were significant differences between the two (t=1.98, 4.77, all P<0.05). Conclusions The degree of education is a protective factor for mild cognitive impairment. Coronary heart disease is an independent risk factor for mild cognitive impairment, and it mainly impairs delayed memory ability.

Objective To observe the effect of electroacupuncture at thoracic Jiaji points (EX-B 2) on the Behavior Measurement Scale (BMS) score, and expressions of nestin and glial fibrillary acidic protein (GFAP) in spinal cord in mice after spinal cord injury, and to explore the action mechanism of electroacupuncture in protecting nerves and recovering motor function.Method Ninety-six female C57BL/6 mice were randomized into a sham operation group, a spinal cord injury group, an electroacupuncture group, and an acupuncture group, 24 mice in each group. Except for the sham operation group, spinal cord injury at T9 level was induced in the other three groups. The electroacupuncture group was intervened by electroacupuncture at Jiaji points (EX-B 2) of T7 and T11, and the acupuncture group received manual acupuncture at the same acupoints. The intervention was conducted 15 min each time, once a day, with 1 d interval every 5 d, for a total of 28 d. The BMS score was evaluated respectively after 3 d, 7 d, 14 d, and 28 d intervention. Enzyme-linked immunosorbent assay (ELISA) was adopted to detect the expression ofnestin in spinal cord, and immunofluorescence and Western blotting were used to detect the expression of GFAP in spinal cord.Result Respectively 14 d and 28 d after spinal cord injury, the main and auxiliary scores of BMS in the acupuncture group were significantly higher than those in the spinal cord injury group (P<0.05); 28 d after spinal cord injury, the main and auxiliary scores of BMS in the acupuncture group were significantly higher than those in the spinal cord injury group (P<0.05) and the auxiliary BMS score in the electroacupuncture group was significantly higher than that in the acupuncture group (P<0.05). Respectively 3 d, 7 d, and 14 d after spinal cord injury, the contents of nestin in the electroacupuncture group were obviously increased and significantly different from those in the spinal cord injury group (P<0.05). The nestin content of the acupuncture group was significantly higher than that of the spinal cord injury group 7 d and 14 d after spinal cord injury (P<0.05). The expression of GFAP in the electro-acupuncture group was significantly higher than that in the spinal cord injury group 3 d and 7 d after spinal cord injury (P<0.05); the inhibitions on the immunoreactivity of GFAP in the electroacupuncture group and acupuncture group were more significant than the inhibition in the spinal cord injury group 28 d after the injury (P<0.05).Conclusion Electroacupuncture at Jiaji points (EX-B 2) can promote the recovery of motor function after spinal cord injury, which is related to the enhancement of the expressions of nestin and GFAP within 7 d after the injury, and the two expressions are in a positive correlation. In the early stage of treatment, electroacupuncture can boost the activity of astrocytes to act as neural stem cells and inhibit the immunoreactivity of GFAP in the later stage to benefit the reconstruction of neurologic function.

This paper reports a method to preserve toxoplasma gondii in the washing fluid of normal caline from the peritoneal cavity of mice in a refrigerator at 4℃. The washing fluid continued to infect the mice when it had been preserved for 49 days. Therefore, the mice san be vacunated through the peritoneal cavity about a month later, and then the washing fluid drawn from the peritoneal cavity and preserved in the refrigerator at 4℃ again. This method helps preserve toxoplasma gondii for a long time. This method is convenient, as compared with other methods.

The citations of original foreign language books in doctorial dissertations of Suzhou University in 2013 were analyzed,which showed that the number of cited original foreign language books on humanities and social sciences was greater than that on other science, the cited books were not limited to those that were recently published, the number of cited original foreign language books published by university press was greater than that published by other publishers. Suggestions were put forward for improving the acquisition of original foreign language books.

Iron metabolism is involved in cancer initiation, progression, metastasis, and angiogenesis.Ferritin,ferritin receptor,transferrin, and transferrin receptor are often overexpressed in cancers. Not only iron deprivation can inhibit the growth of cancers,but also may suppress matrix metalloproteinases-related metastasis.