Objective To study the effects of ulinastatin on proinflammatory cytokine and oxygen free radicals during acute lung injury in rabbits. Methods Eighteen health New Zealand rabbits were randomly divided into 2 groups: oleic acid group(n=9) and ulinastatin group(n=9). The rabbits of oleic acid group were intravenously injected analytical pure oleic acid(0.12ml/kg) to set up a model of acute lung injury. The rabbits of ulinastatin group were intravenously injected ulinastatin 50000u/kg before injecting oleic acid and received intravenous infusion of ulinastatin 5000u?kg -1 ?h -1 after injecting oleic acid. Before injecting oleic acid and 1h, 2h, 3h and 4h after injecting oleic acid,venous blood samples were taken to determine the concentration of plasma IL-8, MDA and active oxygen,and arterial blood samples were also taken for gas analysis. Results The concentration of IL-8, MDA and active oxygen and PaCO 2 gradually increased,and PaO 2 gradually decreased after injecting oleic acid in the both groups of rabbits. The changes of the above indices in the oleic acid group were larger than those in the ulinastatin group, and the difference between the two groups was significant. Conclusion Ulinastatin inhibited the release of proinflammatory cytokine and reduced the production of oxygen free radicals during acute lung injury.

Objective To explore the clinical effect of autologous fat grafting in frontotemporal augmentation.Methods The fat granule was harvested from patient's abdomen or both medial knees by a multi-holed liposuction cannula attached to a syringe with negative pressure.Then the syringe was held vertically with the open end pointing downward until the fat and fluid were separated.The aqueous layer at the bottom and the oil layer on the top were discarded.The fat without being washed was directly transferred into 2 ml syringes and injected into bilateral temple and frontal depression area with a multi-plane,multi-tunnel,multi-point approach.The volume of injection was over 20％-30％.From March 2010 to March 2013,117 patients underwent this procedure.Results In all the 117 cases,there were no complications such as hematoma,induration,and infection.There were 11 cases (11.4％) in temple and 13 cases (20.0％) in frontal region achieved aesthetically-acceptable results after the second treatment.There was no statistically significant difference in secondary operation rates between the temple group and the frontal grouup (x2 =2.229,P＞0.05).Based on the followup for 3-36 months on all 117 cases,satisfactory results were obtained with improved frontotemporal contour and skin texture.The volume retention of transplanted fat was estimated between 60％ and 80％,which was based on subjective analysis of photographs.Conclusions Autologous fat grafting in frontotemporal depression is a convnient technique with reliable clinical results.It is also an optimal and safe method for facial rejuvenation and contouring remodeling.