1.The development of an intelligent EEG monitor
Chinese Journal of Medical Physics 2001;18(1):54-55
This paper introduces consistents of an intelligent EEG monitor, including its hardware and software. Emphasis is put on the discussion about the detection of epilepsy waves with wavelet transform. At last, analysis of clinical EEG data show that this system has achievedhighsensitivity and selectivity.
2.Concentration of Some Minerals in Whole Saliva from Patients with Adult Periodontitis
Xiaolin TANG ; Yaping PAN ; Zhaoyuan WANG
Journal of China Medical University 2001;30(1):66-68
Objective: Our aim was to analyze the relationship between the concentration of some minerals in saliva and periodontitis.Methods:Twenty-five patients with adult periodontitis (AP group), 25 age and sex matched healthy people(N group) were selected. Unstimulated whole saliva samples were collected and qualified for the concentration of TCa,P,Mg2+, Na+,K+, and Cl-. Clinical parameters on periodontitis were also assessed for the patients. All the aforementioned indices were measured repeatedly at the end of the fourth week after initial therapy. Results:The clinical condition of the patients improved significantly after treatment. The level of saliva Ca elevated before and after treatment in AP group than that in N group. Before treatment, the concentration of Na+ also increased significantly, but it decreased to normal level after initial therapy. The findings also indicated the positive relationship between the concentration of salivary Na+ and pocket probing depth as well as gingival index before treatment.Conclusion:The elevated level of saliva Ca is a sign of periodontitis and may help the occurrence and progress of periodontitis. The level of saliva Na+ may indicate the pocket depth and degree of gingivitis.The saliva Na+ is closely associated with the clinical condition of periodontitis.
3.Effect of Candida albicans on cell cycle distribution of KB cells
Ling ZHANG ; Xiaolin TANG ; Zhaoyuan WANG
Journal of Practical Stomatology 2001;0(01):-
Objective:To investigate the effects of Candida albicans(C. albicans) on the cell cycle distribution of oral epithelial KB cells in vitro. Methods:KB cells at 3?106/ml were seeded and cultured for 48 h, then the bacterial filament and the spore of C. albicans at 102 CFU/ml were added to the cultures respectively. KB cell culture without C. albicans was used as the control. After 48 h culture cell suspensions were prepared and subjected to FACS flow cytometry for detecting the cell cycle distribution. Results:Filament of C. albicans decreased the percentage of G0/G1 cells, increased G2/M cell population and proliferation index of the cells(P
4.Relationship between the expression of EGFR,C-erbB-2 proteins and the lung metastatic potential of salivary adenoid cystic carcinoma cell lines
Jie MA ; Ming ZHONG ; Zhaoyuan WANG
Journal of Practical Stomatology 2001;0(03):-
Objective:To investigate the expressions of epidermal growth factor receptor(EGFR) and C-erbB-2 in two salivary adenoid cystic carcinoma cell lines with different metastatic potential. Methods:Salivary adenoid cystic carcinoma SACC-83 cell line and its lung metastatic cell line SACC-LM were cultured in vitro, their cytoplasm and membrane samples were extracted, and the expressions of EGFR and C-erbB-2 were detected by Western blot, the results were quantitatively analyzed by FluorChem V2.0 software.Results:EGFR expression level in cell membrane was higher than that in cytoplasm(P0.05) in both cell lines.Conclusion:Higher EGFR expression in cytoplasm and higher C-erbB-2 expression may be related to the metastatic potential of SACC-LM cells.
5.The expression of MMP-8 in dental pulp during root development of permanent teeth
Panyu ZHAO ; Shujie LIU ; Zhaoyuan WANG
Journal of Practical Stomatology 1995;0(04):-
0.05, group 2 vs group 3 P
6.The trend of stomach neoplasms mortality in Zhaoyuan County, from 1970 to 2004.
Nan-Da WANG ; Cheng-Yang SUN ; Yu-Ting ZHAI
Chinese Journal of Preventive Medicine 2007;41 Suppl():73-76
OBJECTIVETo understand the trend of gastric cancer mortality based on the exploration of the death data from 1970 to 2004 in Zhaoyuan County.
METHODSThe death data of 1970-1974 were collected from the first malignant tumor death survey of China, and the data of 1985-2004 were derived from the cancer registration network of Zhaoyuan. The appreciation indexes were crude mortality rate and standardized mortality rates by the percentages of Chinese population in 1982 and the percentages of world population in 1966.
RESULTSThe crude mortality rates in the five periods of 1970-1974, 1985-1989, 1990-994, 1995-1999, and 2000-2004 were 34.94, 44.34, 40.05, 48.73, and 40.18 per 100,000, respectively; the standardized mortality rates by Chinese population were 30.82, 30.35, 27.78, 31.63, and 20.10 per 100,000, respectively. The gastric cancer mortality remained stability from 1970 to 1999, however, the standardized mortality rate of gastric cancer in 2000-2004 declined by 34.8% compared with that in 1970-1974. The study also observed that the highest age-specific gastric cancer mortality, in the past 35 years, has been postponed by 10 years. And, the gastric cancer mortality ranked from top first to top three.
CONCLUSIONThere is a decreasing trend for the incidence of stomach cancer and this might be associated with the changes of dietary consumption and pattern.
Adolescent ; Adult ; Age Distribution ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Male ; Middle Aged ; Mortality ; trends ; Registries ; Retrospective Studies ; Stomach Neoplasms ; mortality
7.Research on standardized management of quality control for medical equipment
Feiyi CUI ; Hongwen CHEN ; Zhaoyuan WANG ; Shaozhou YANG
Chinese Medical Equipment Journal 2017;38(5):138-140,144
Objective To discuss the standardized management strategies of medical equipment quality control in the whole life cycle of medical equipment.Methods The quality control was prepared from the aspects of management,technology and materials firstly,then quality control object was determined through a comprehensive risk rating system with quantitative values at risk,and overall analysis was executed with PDCA method finally.Results The management of medical equipment quality control was further standardized,the risks during medical equipment utilization were reduced,and the comprehensive benefit of the hospital was increased.Conclusion The standardized management of medical equipment quality control is of great importance to enhance medical quality.
8.The correlation between basic fibroblast growth factor and extracellular matrix in dental pulp during root development of permanent teeth
Xu CHEN ; Shuli LIU ; Shujie LIU ; Jiandong ZHU ; Zhaoyuan WANG
Journal of Practical Stomatology 2000;0(06):-
Objective: To detect the expression of basic fibroblast growth factor(bFGF), type Ⅲ collagen(Ⅲ-col)and fibronectin(FN) in human dental pulp at different root development stages of permanent teeth. Methods: 10 teeth at stage Ri~R 1/4 ,15 at R 1/3 ~R 3/ 4 and 12 at Rc~Ac were sectioned for following examination.bFGF, Ⅲ-col and F N were qualitatively and quantitatively analyzed by the use of immunohistologica l SP method and image analysis technique. Results: The ex pression of bFGF became gradually weaker and weaker with the development of too th root (P
9.Expression of human telomerase reverse transcriptase and bcl-2 in ameloblastoma.
Jie WANG ; Ming ZHONG ; Zhaoyuan WANG
West China Journal of Stomatology 2003;21(6):441-443
OBJECTIVETo study the expression of telomerase reverse transcriptase (hTERT) and bcl-2 in ameloblastoma (AB), METHODS: hTERT mRNA in 54 cases of AB (primary AB 31 cases, recurrent AB 17 cases, malignant AB 4 cases) and 7 cases of oral normal mucosa was detected by in situ hybridization, and bcl-2 by S-P method.
RESULTSThe expression of hTERT mRNA was negative or weak in normal oral mucosa (14.3%), moderate or strong in AB (94.4%). There was a significant difference in these two groups (P < 0.001). The difference between the expressions of hTERT in primary, recurrent and malignant AB was significant (P < 0.05). The positive ratio of bcl-2 in AB and normal oral mucosa was respectively 88.0%, 44.4%. There was a significant statistical difference in these two groups (P < 0.001). hTERT mRNA was stronger in recurred or malignantly transformed AB (P < 0.05).
CONCLUSIONThe expression of hTERT and bcl-2 is stronger in recurred or malignantly transformed AB, and it could be used as an indicator of AB prognosis. Telomerase activity and bcl-2 expression play an important role in genesis and development of AB.
Adolescent ; Adult ; Ameloblastoma ; enzymology ; metabolism ; Biomarkers, Tumor ; Child ; DNA-Binding Proteins ; Female ; Humans ; Jaw Neoplasms ; enzymology ; metabolism ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; enzymology ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; biosynthesis ; genetics ; RNA, Messenger ; biosynthesis ; genetics ; Telomerase ; biosynthesis ; genetics
10.Sodium dodecyle sulphate polyacrylamide gel electrophoresis of whole salivary proteins from patients with adult periodontitis.
Xiaolin TANG ; Yaping PAN ; Zhaoyuan WANG
West China Journal of Stomatology 2003;21(2):98-100
OBJECTIVEThis study was intended to determine the salivary protein factors related to adult periodonititis.
METHODSTwenty-five patients with adult periodontitis (AP group) and twenty-five normal controls (NC group, 25) were adopted in the study. Salivary protein composition in unstimulated whole saliva samples obtained before and after basic treatment was analyzed by SDS-PAGE.
RESULTSThe volume of proteins with 66 kD, 18 kD, 15 kD, 13 kD in AP group before treatment were all remarkably higher than those in NC group. In addition, 51 kD, 34 kD, 19 kD, 17 kD protein bands were more frequently observed in AP group by comparison with NC group. However, after initial therapy, all the variables mentioned above decreased remarkably.
CONCLUSIONThe results demonstrated that protein bands with 18 kD, 15 kD, 13 kD might be closely related to the occurrence and recovery of periodontitis, and serum-derived proteins in saliva increased in patients with adult periodontitis.
Adult ; Electrophoresis, Polyacrylamide Gel ; methods ; Female ; Humans ; Male ; Middle Aged ; Periodontitis ; metabolism ; Saliva ; chemistry ; Salivary Proteins and Peptides ; analysis ; Sodium Dodecyl Sulfate