Aim To investigate the effects of berberine on extracellular matrix in rat glomerular mesangial cells cultured under high glucose and its possible mechanism.Methods Six groups were divided according to the different experimental conditions:① Normal glucose group (NG);② Mannitol group (Mannitol);③ High glucose group (HG);④ SB203580 treatment group (HG+SB203580);⑤ Berberine low dosagegroup (HG+BBR 30 ?mol?L-1);⑥ Berberine high dosage group (HG+BBR 90 ?mol?L-1). The phospho-p38MAPK,phospho-cAMP response element binding protein (CREB) and fibronectin were detected by Western blot analysis. Results Berberine obviously decreased protein expression of fibronectin compared with high glucose group. Phospho-p38MAPK and phospho-CREB level was significantly lowered compared with that of high glucose group. Conclusion Berberine can inhibit extracellular matrix accumulation including fibronectin via p38MAPK signal pathway in treatment of diabetic nephropathy.

0.05, group 2 vs group 3 P

Objective: To detect the expression of basic fibroblast growth factor(bFGF), type Ⅲ collagen(Ⅲ-col)and fibronectin(FN) in human dental pulp at different root development stages of permanent teeth. Methods: 10 teeth at stage Ri～R 1/4 ,15 at R 1/3 ～R 3/ 4 and 12 at Rc～Ac were sectioned for following examination.bFGF, Ⅲ-col and F N were qualitatively and quantitatively analyzed by the use of immunohistologica l SP method and image analysis technique. Results: The ex pression of bFGF became gradually weaker and weaker with the development of too th root (P

To explore the clinical efficacy and possible mechanism of Tai Ji Quan for post-stroke depression (PSD), literature related to Tai Ji Quan and PSD were retrieved from China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full- text Database (Wanfang), Chongqing VIP Database (CQVIP), China Biology Medicine Disc (CBM), and PubMed, screened and then summarized. The results showed that Tai Ji Quan could effectively improve the depression and quality of life of stroke patients, and there were differences in the clinical efficacy among different training programs. Tai Ji Quan has the characteristics of "regulating mind", "regulating breath", and "regulating body". It may achieve the effect of "combined physique-spirit treatment" by improving social psychology, increasing the level of neurotrophin, regulating neuroendocrine, reducing inflammatory factors, and regulating neural circuits.

Objective: To explore the biological characteristics of synovial fluid-derived mesenchymal stem cells (SF-MSCs) cultured in serum-free medium and the ability of in vitro reconstruction of three-dimensional cartilage combined with scaffold material. Methods: Human SF-MSCs were cultured in serum medium and mesenchymal stem cells medium-serum free (MSCM-sf) respectively, then the proliferative ability and morphology of SF-MSCs were compared; The third passage SF-MSCs cultured in MSCM-sf were identified by flow cytometry, three-way(chondrogenic, osteogenic, adipogenic)differentiation assay and induced for chondrogenic differentiation when combined with polyglycolic acid/polylactic acid (PGA/PLA). Results: SF-MSCs cultured in MSCM-sf had better morphology and proliferative ability than that cultured in serum medium. The expression levels of positive markers of the third passage SF-MSCs cultured in MSCM-sf, such as CD73 (99.5%), CD90 (98.9%) and CD105 (96.5%), were more than 95%. However, the overall negative markers (CD34, HLA-DR and CD11b) expressed less than 2%. Three-way differentiation staining was positive. The combination of SF-MSCs and PGA / PLA can be induced into cartilage in vitro. Conclusions SF-MSCs cultured in MSCM-sf can be amplified under the condition of maintaining the stem cell characteristics, and can be combined with PGA/PLA scaffold to construct three-dimensional cartilage in vitro.

Taking Pharm .D .degree education in School of Pharmacy ,University of Pittsburgh as an example ,this pa-per introduced the American pharmacists training system ,degree setting for service-oriented pharmacy students ,training objec-tive ,course design ,teaching staff and other aspects .This paper also reviewed pharmacy education and professional develop-ments for licensed pharmacists in China .Based on the successful experience abroad ,proposals were made to modify the domes-tic pharmacy education .The process of personnel training should be emphasized to improve the quality and capability of licensed pharmacists in China .

Objective To investigate the practices and experience in the organization and implementation process of prac-tical training drill for medical supply support .Methods To review and comment on the overall design ideas ,specific steps , practical effect and experience of medical supply support in practical training drill .Results Through six years of exploration and practice , we established a training method of implementation of practical training drill for medical supply support . Conclusion It is right and effective in the organization and implementation of practical training drill for medical supply support . It is necessary to continue improving the mode of organization and implementation with combination of practical teaching and re-search .

Objective To introduce the process and experience of construction of medical supplies support practice train-ing platform under informatization.Methods To review and comment the structure composition,functions implemented,ap-plications and experience of medical supplies support practice training platform.Results The medical supplies support practice training platform achieved four functions which are indoor deduction,simulation training,drill outdoor,participate in military health service exercise.Conclusion The medical supplies support practice training platform has achieved good effects in teach-ing and training,which has effectively promoted the teaching and training mode reform aimed at combat requirements.

Objective:To evaluate the teaching effect of Emergency Nursing practice course using scenario simulation teaching combined with formative assessment. Methods:The subjects were selected from vocational nursing students of Batch 2015 (control group, n=49) and Batch 2016 (intervention group, n=40). The control group adopted the scenario simulation teaching method, and the intervention group added formative assessment on the basis of simulation teaching. The end-of-term theoretical scores of Emergency Nursing and the scores of students' general self-efficacy scale were used as evaluation tools. SPSS 22.0 statistical software was used for data processing and analysis. Results:The scores of the intervention group (85.20±8.05) were higher than those of the control group (79.73±8.46), with statistical significance ( P<0.05). The scores of self-efficacy in both groups were improved, while the scores of the control group after the course (2.68±0.42) were higher than those before the course (2.61±0.47), but there was no statistical significance. The scores of the intervention group after the course (2.78±0.43) were higher than those before the course (2.62±0.43), with statistical significance. Conclusion:The application of scenario simulation teaching combined with formative assessment in the practical course of Emergency Nursing can be helpful to improve students' self-efficacy and comprehensive ability of first aid.

OBJECTIVEThis study was to investigate the clinical and pathological characteristics, curative effect and prognosis of primary central nervous system lymphoma (PCNSL).

METHODSThe clinical and pathological features, diagnosis and treatment, curative efficacy and survival rate of 20 cases of primary central nervous system lymphoma were retrospectivaly analyzed.

RESULTSThe indicated that the clinical manifestations included headache, paralysis, seizures, dizziness and mental acuity, etc. There were 7 cases with single focus of lesion, 13 cases with multiple foci of lesions. The pathologic examimation showed 1 case of peripheral T-cell lymphoma and 19 cases of diffuse large B cell lymphoma, they all were from non-germinal center. Out of 20 cases, 11 cases with Ki-67>75%,6 cases with Ki-67 50%-75%, 2 cases with Ki-67 25%-50% and 1 case with Ki-67 10%. Therapeutic evaluation were as follows: 10 cases with CR, 2 cases with PR, 6 cases with PD in 18 patients recieved treatment. The use of ritusimab in treatment or no use and number of lesion foci in cranium were related to survival time (P < 0.05) . The survival time in group with Ki-67(+)<75% was longer than that in group with Ki-67(+)>75% (P = 0.042). Age and CSF-TP level were related to curative effect (P < 0.05) .

CONCLUTIONThere is no specific clinical manifestation. The physical examination and imageological diagnosis of PCNSL, as well as pathological biopsy are necessary for diagnose of PCNSL. The factors for poor prognosis include age > 60 years, CSF-TP > 600 mg/L, multiple lesions and Ki-67(+)>75%. PCNSL has high malignancy and poor prognosis. The chemotherapy based on high dose of MTX (among which MTX combined with rituximab) and HSCT after CR are the best treatment regimen for DLBCL.