ObjectiveTo study the effect of berberine on diabetes or insulin resistance accompanied with reduction of skeletal moscle and wasting in db/db mice.Methods db/db mouse-a model of diabetes/insulin-resistance was studied,with the wild type mouse as control.After being treated with berberine ( 5 mg · kg-1 · d-1 ) for 3 weeks,the muscle size of tibia anterior (TA) of the animals was measured after staining with laminine/Hyosin Heavy chain-Slow using immunochemistry,then observed under fluorescent microscope and calculated with software.The rate of [ 14 C ] -Phenalanine incorporation into the uuscle was measured to analyze the protein synthesis,and the [ 3 H ] -Tyrosine released into the medium was determined in order to analyze protein degradation.The mRNA expressions of muscle atrophy Fbox-1 ( Atrogin-1 ) and muscle ring finger-1 ( MuRF-1 ) were measured by Northern blot.Results With berberine treatment,blood glucose and fat levels were lowered [ ( 18.55 ± 3.79 vs 26.32 ± 4.02 ) mmol/L,P＜0.01 ; ( 2.75 ± 0.30 vs 3.77 ± 0.52 ) g,P＜0.05 ],but tibia anterior muscle weight/length ratio and cross-section area were decreased,rates of protein synthesis in isolated muscles of db/db mice were decreased by 18％ -22％,and the rates of degradation were significantly raised by 24％ -26％ after berberine treatment.There also was increased the transcription and translation of Atrogin-1 and MuRF-1,accompanied with decreased eukaryotic initiation factor 3 subunit (eIF3-f)protein level simultaneously. ConclusionBerberine improves hyperglycemia and insulin resistance by down-regulating blood sugar and body fat,but it causes reduced protein synthesis and minimally enhanced protein degradation.The mechanism might be related to berberine-induced up-regulating Atrogin-1 、MurF-1 and down-regulating eIF3-f.

Objective To evaluate the effect of double stapling technique for sphincter preservation operation in mediate-low rectal carcinoma.Methods The resection through double stapling technique for sphincter preservation approach was performed on 30 patients with mediate-low rectal carcinoma from January 2004 to November 2007.The distance between the anal verge and the lower margin of the tumor was 4～8cm(averaged 6.5 cm),including 22 patients in Dukes A stage,6 Dukes B and 2 Dukes C.Results Fecal continences were preserved successfully in all patients.2 cases had got anastomotic narrowing(6.6%) after operation.At a median follow-up of 46 months,two patients had local recurrences(6.6%).There was no urinary dysfunction in this series.Conclusion Double stapling technique for sphinceter preservation operation was feasible and safe,provide a original operative style for mediate-low rectal carcinoma.

Objective To evaluate the effect of procaine on prevention of skin slap necrosis after modified radical mastectomy in breast cancer.Methods 106 breast cancer patients were randomly divided into treatment and control groups.In the treatment group,procaine(1% 100ml diluted in 300ml mormal saline,42℃)were compressed under the skin flap in operation,the control group received same amount of normal salin instead.The rates and sizes of skin flap necrosis were obeserved and compared.Results There were 13 cases suffured from skin flap necrosis among 106 breast cancer cases who received surgery operation.The rate of the flap necrosis in treatment group was5.76%(3/52),and the control group was 18.51%(10/54),there was significant difference(P

Objective To compare and analyze the effects of preoperative respiratory function exercise on postoperative pulmonary function and complications in elderly patients who undergoing abdominal surgery . Methods A total of 92 elderly patients who underwent abdominal surgery were recruited .They were randomly divided into observation group(46 cases) and control group(46 cases) according to the random number table method . The control group was treated with routine preoperative care methods , the observation group was given preoperative respiratory exercise on the basis of the control group .The pulmonary function tests were performed pre and post surgery.Then,the postoperative pulmonary complications ,VC,FEV1,FVC,MVV and the average length of hospital stay of the two groups were recorded and compared .Results After operation,the levels of VC,FEV1,FVC and MVV in the control group were (2.48 ±0.71)L,(51.40 ±10.10)L,(1.79 ±0.17)L,(62.40 ±13.00)L/min,respectively, which in the observation group were (2.89 ±0.72) L,(63.20 ±9.60) L,(2.21 ±0.16) L and(73.30 ± 12.70)L/min,respectively,the differences between the two groups were significant (t =2.750,5.743,12.202, 4.068,all P<0.05).The average hospitalization time in the observation group was (11.62 ±1.41) d,which was significantly shorter than (15.56 ±6.23) d in the control group (t =4.163,P <0.001).The incidence rate of pulmonary infection in the control group was 26.09％,which was significantly higher than 8.69％ in the observation group(χ2 =4.842,P<0.05).Conclusion The respiratory function training can effectively reduce the incidence of postoperative pulmonary complications in elderly patients with abdominal surgery ,improve the patients ' postoperative pulmonary function, and shorten the patients ' hospital recovery time .Respiratory function exercise is a simple method,and is worthy of promotion in related fields .