Biochemistry is an important basic medical course, and it is also a difficult course.In order to improve the comprehensive ability of seven-year program clinical medical students in Capital Medical University, teaching reform of Biochemistry was carried out.Teaching content was redesigned to strengthen the learning of basic knowledge of Biochemistry and to increase the depth of teaching content, while expanding the content by introducing new achievements and new technology to keep up with the forefront in the development of Biochemistry.Various methods such as the comparative method, case teaching and so on, were used flexibly in the teaching practice in order to improve teaching effects.The experiments carried out in the course were also redesigned and integrated, some distinctive experiments such as designing experiments, comprehensive experiments and innovative experiments were opened to improve students' hands-on ability and to cultivate students' scientific thinking.

With the increasing international exchange of medical education , frequent exchanges increasingly of medical personnel through the cross-border and trans-regional .It caused widespread concern about the quality of medical education in different countries .This review focus on quality performance on the certification requirements of the standard of medical education .We take it as an opportunity to assess international medical education accredi-tation, and promote the reformation of teaching and examination .We tried to explore and establish the full quality assessment system for biochemistry .

Objective To analyze the effect of different treatment conditions on cells synchronization in G0/G1 phase to get the best con-dition, and to explore its effect on neural differentiation of bone marrow mesenchymal stem cells (BMSCs) induced by basic fibroblast growth factor (bFGF). Methods BMSCs were isolated and cultured in 5%, 1%, 0.5%, 0.1%, 0 fetal bovine serum (FBS) respectively, for 24 hours and 48 hours. After PI staining, cell cycle proportions of each phase were detected by flow cytometry, and were compared with the normal group (10%FBS). After the optimal treatment condition was got, 20 ng/ml bFGF was added into synchronization group and unsyn-chronization group 3 days and 7 days, respectively. The expression of Nestin and Tuj-1 were detected with immunofluorescence. Results Adult rat BMSCs were isolated from bone marrow and cultured, after passage, the cells were with long spindle shape. Compared with the normal group, the cell proportion of G1/G0 phase increased under different treatments, peaked with (94.274 ± 0.468)%under 1%FBS, 48 hours (F=39.91, P<0.001). After bFGF induction for 3 days, the Nestin+cell number was higher in the synchronization group than in the un-synchronization group [(80.3 ± 2.4)%vs. (12.1 ± 1.5)%] (F=28.25, P<0.001). After bFGF induction for 7 days, the Tuj-1+cell number was higher in the synchronization group than in the unsynchronization group [(74.8±3.2%)%vs. (19.3±2.5)%] (F=17.95, P<0.001). Conclusion 1%FBS, 48 hours is the optimal condition to BMSCs synchronization in G0/G1 phase, which can promote the neural differentiation of BM-SCs.

Objective To observe the effect of the health education and skills training for caregivers on health status of stroke patients and their family caregivers. Methods Hospitalized stroke patients were divided into the intervention group (n=38) and the control group (n= 36). Both groups received rehabilitation training for 1 month in hospital. The family caregivers of intervention group received Timing It Right education and skills training in addition. All the patients were assessed with the European Stroke Scale (ESS), Mini-Mental State Examination (MMSE), simple Fugl-Meyer Assessment (FMA), modified Barthel Index (MBI), and the caregivers were assessed with the General Health Questionnaire (GHQ-12) before and 3 months after intervention. Results The scores of ESS, FMA, MBI improved more in intervention group than in the control group after intervention (P<0.05), as well as their caregivers in the items of playing a useful part, under stress, overcoming difficulties, enjoying normal activities, facing up to problems, feeling unhappy and depressed, lossing confidence, thinking of self as worth of GHQ-12 (P<0.05). Conclusion Health education and skills training for family caregivers can improve the health of caregivers, and further improve the function outcome of stroke patients.

OBJECTIVETo explore the role of myeloperoxidase (MPO) and tumor necrosis factor-α (TNF-α) in myocardial injury induced by hind-limb ischemia-reperfusion (IR) in rats.

METHODSRat models of bilateral hindlimb IR established using a tourniquet were randomized into 9 groups, including a normal control group normal, 2 ischemic groups with hindlimb ischemia for 2 and 4 h, and 6 IR groups with a 4-h ischemia followed by reperfusion for 0.5, 2, 4, 6, 12, and 24 h. The plasma and myocardial levels of MPO and TNF-α in each group were measured, and the myocardial expression of TNF-α was determined with immunohistochemistry.

RESULTSCompared with the normal control group, the rats with a 2-h ischemia showed significantly increased levels of MPO and TNF-α in the plasma and myocardium. Compared with those in rats with a 4-h ischemia, the plasma and myocardial MPO levels increased significantly at 0.5 and 2 h of reperfusion, respectively; the plasma TNF-α level increased significantly at 4 h of reperfusion and myocardial TNF-α level decreased obviously at 12 h; plasma levels of MPO and TNF-α both significantly decreased at 24 h. The plasma MPO and TNF-α and myocardial TNF-α reached the peak levels at 4 h of reperfusion, and the peak myocardial MPO level occurred at 6 h. Immunohistochemistry showed that TNF-α positivity moderately increased after hindlimb ischemia, and further increased at 4 h of reperfusion but obviously reduced at 24 h.

CONCLUSIONThe activation of systemic and local neutrophils and inflammatory cytokines may play an important role in myocardial injury induced by hindlimb IR in rats.

Animals ; Disease Models, Animal ; Hindlimb ; blood supply ; Ischemia ; metabolism ; Male ; Myocardium ; metabolism ; Peroxidase ; metabolism ; Rats ; Rats, Wistar ; Reperfusion Injury ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

OBJECTIVETo assess the protective effect of exogenous hydrogen sulfide (H₂S) against myocardial injury after skeletal muscle ischemia/reperfusion (IR) in rats and explore the mechanism.

METHODSThirty-one Wistar rats were randomized into normal control (n=8), IR group (n=8, with a 4-h reperfusion following a 4-h ischemia of the bilateral hindlimbs induced using a tourniquet), NaHS group (n=8, with IR and intraperitoneal injection of 14 µmol/kg NaHS), and DL-propargylglycine (PPG) group (n=7, with IR and intraperitoneal injection of 50 mg/kg PPG). The plasma levels of CK-MB and the levels of MPO, TNF-α, MDA, T-SOD, and CuZn-SOD in the plasma and myocardial tissues were measured. The expression of TNF-α in the myocardium was examined using immunohistochemistry. RESULTS Skeletal muscle IR induced significantly increased plasma CK-MB level (P<0.05) and the levels of MPO, TNF-α, and MDA in the plasma and myocardium, and significantly decreased plasma and myocardial levels of T-SOD and CuZn-SOD (P<0.05). NaHS treatment significantly decreased plasma CK-MB level (P<0.05), reduced plasma and myocardial levels of MPO, TNF-α, and MDA, and increased plasma and myocardial T-SOD and CuZn-SOD in rats with IR (P<0.05), whereas PPG treatment did not produce any obvious responses (P>0.05). Immunohistochemistry showed an obviously reduced expression of TNF-α in the myocardium in rats with NaHS treatment compared with those in IR group.

CONCLUSIONH₂S treatment can alleviate myocardial injury induced by skeletal muscle IR in rats by inhibiting the inflammatory cytokines and oxidative stress.

Animals ; Creatine Kinase, MB Form ; metabolism ; Disease Models, Animal ; Hydrogen Sulfide ; pharmacology ; Ischemia ; metabolism ; Male ; Malondialdehyde ; metabolism ; Muscle, Skeletal ; blood supply ; metabolism ; Myocardial Reperfusion Injury ; metabolism ; prevention & control ; Oxidative Stress ; Peroxidase ; metabolism ; Rats ; Rats, Wistar ; Superoxide Dismutase ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism

Public hospitals should be centered on people's health and guided by public welfare.In the publicity work,how to better meet the public's demand for health information has become an important issue.Based on the the Gatekeeper Theory,this paper analyzes the necessity of the"Two-Way Gatekeeper"of the publicity department in health communication from the attrib-utes of medical news content,medical journalists'education background and practice situation,and discusses the practical applica-tion of popular science and professional supervision by the publicity department in public hospital health communication,providing new exploration for the brand communication of public hospitals and helping hospitals to achieve high-quality development.

As the main body of the national healthcare service system,public hospitals play a crucial role in implemen-ting the Healthy China strategy.Therefore,promoting the deep integration of Party building and business operations has become an inevitable trend for public hospitals in the new era to strengthen Party work and achieve high-quality development.The Sun Yat-sen University Cancer Prevention and Treatment Center focuses on the requirements of grassroots Party building,emphasizing the top-level design and refined management of the comprehensive goal management system of Party branches.Through scientific and effective comprehensive goal management,it harnesses high-quality Party building to lead the high-quality development of public hospitals.This study actively explores effective pathways for the deep integration of Party building and business operations in public hospitals,providing insights and references for promoting high-quality development in these institutions.

With the rapid development of information technology and the advent of the big data era,promoting smart Par-ty building has gradually become an inevitable trend and important approach for strengthening the Party building effort in public hospitals in the new era.Sun Yat-sen University Cancer Center,focusing on the requirements of grassroots Party building and in-tegrating the characteristics of Party building work in university-affiliated hospitals,has innovatively developed a smart Party building information platform suitable for the ecosystem of public hospital Party building.The platform's functional modules are designed based on the closed-loop process of Party building work assessment,which includes"task issuance-execution-evalua-tion-rectification".It has been found that the orderly construction of the smart Party building information platform has signifi-cantly contributed to enhancing the standardization of Party branch construction,facilitating burden reduction and empowerment of grassroots organizations,and strengthening the leading role of Party building work.This initiative serves as a viable practical ex-ample for the intelligent operation of hospital Party building work and spurs continuous innovation and high-quality development in party building efforts in university-affiliated hospitals.

Objective To determine the feasibility of neck vessel wall imaging technology with three?dimensional variable?flip?angle turbo spin?echo (3D T1w?SPACE) for the detection of carotid atherosclerotic disease before revascularization. Methods Thirty?one patients who underwent carotid endarterectomy (CEA) and fifty?three patients who underwent carotid stenting (CAS) were enrolled prospectively. Neck vessel wall imaging examination were performed in all patients whilecarotid artery DSA were performed in all CAS patients. Quantitative measurements including stenosis, lesion length, and the presence or absence of plaque ulceration obtained with 3D T1w?SPACE and DSA were independently determined. And images of the 3D T1w?SPACE were compared with corresponding histology to identify major plaque components including intraplaque hemorrhage (IPH), lipid rich necrotic core (LRNC), and calcification (CA). The consistency rate, sensitivity, specificity, positive predictive value and negative predictive value were used to assess diagnostic value. Bland?Altman plots, intraclass correlation coefficient (ICC), and Cohen Kappa were determined. Results DSA was served as the reference standard. There was an excellent correlation between 3D T1w?SPACE and DSA images in measuring stenosis (r=0.984, P<0.01) and luminal stenosis [ICC=0.98 (95% confidence interval: 0.96-0.99)]. Bland?Altman plots showed that the two examinations were in good consistency in evaluating the extent of stenosis. Sensitivity (89.5%) and specificity (95.1%) was high in 3D T1w?SPACE images compared to DSA for the detection of ulcers. The consistency rate between 3D T1w?SPACE images and histological results for IPH, LRNC and CA detection were 85.7%, 82.1% and 92.9%, respectively. Sensitivity and specificity were 90.0% and 75.0% for IPH;83.3% and 80.0% for LRNC; 91.3% and 100.0% for CA respectively. However, lesion length measurements by using 3D T1w?SPACE were longer than those measured by using DSA (P<0.01).Conclusion Neck vessel wall imaging technology with 3D T1w?SPACE is a noninvasive and accurate technique for the diagnosis of carotid artery atherosclerotic disease before revascularization.