1.Research progress of delayed medical treatment in cancer patients and its influencing factors
Zhaoxuan MENG ; Chong LI ; Xin ZHAO ; Yannan FAN
Chinese Journal of Modern Nursing 2023;29(36):5025-5029
Malignant tumor is a serious threat to human survival and development, and the delay of medical treatment for cancer patients has an effect on the survival rate of patients. This paper summarizes the definition of delayed medical treatment and the current situation, influencing factors and prevention and control countermeasures of delayed medical treatment in cancer patients, so as to provide references for medical personnel to formulate relevant measures of delayed medical treatment.
2.Rapid detection of high-risk HPV16 and HPV18 based on microchip electrophoresis
Zhaoxuan FAN ; Xiao FENG ; Weifei ZHANG ; Xueji ZHANG ; Jin-Ming LIN
Journal of Pharmaceutical Analysis 2020;10(4):329-333
Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by poly-merase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection, then type-specific primers were further used to evaluate the specificity of MCE method. The results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18, and also enabled simultaneous detection of multiplex samples. This MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing results. The inherent capability of MCE facilitated HPV detection conducted in a small chip with automated, high throughput, massive parallelized analysis. We envision that MCE method will definitely pave a way for clinical diagnosis, and even on-site screening of cervical cancer.