1.Analysis on clinical infection distribution and drug resistance of 442 strains of Escherichia coli
Xuelian FANG ; Haiviao LIN ; Huilun HUANG ; Shuyuan WANG ; Zhaoxin LAI ; Shaokui TAN ; Yongzhong RONG ; Jianving WANG
International Journal of Laboratory Medicine 2014;(23):3210-3211,3214
Objective To analyze the clinical infection distribution and drug resistance status of 442 strains of Escherichia coli to provide the basise for the treatment of Escherichia coli infection and the control of nosocomial infection.Methods The clinically submitted various kinds of specimens during 2013 were performed the bacterial culture and identification.The susceptibility of Escherichia coli to commonly used bacterial drugs were detected by adopting the MIC method.The data were analyzed by WHO-NET V5.5 and SPSS V13.0 softwares.Results 442 strains of Escherichia coli were isolated from the middle urine and secretion. The detection rate of ESBLs-producing Escherichia coli was 61.3%.442 strains of Escherichia coli had the high resistance to peni-cillins,cephalosporins and fluoroquinolones,better sensitivity toβ-lactam/β-lactamase inhibitor compounds and highest sensitivity to carbapenems.ESBLs-producing Escherichia coli had the higher resistance to commonly used antibacterial drugs than non-ESBLs-producing Escherichia coli .Conclusion The drug resistance of Escherichia coli is severe.ESBLs-producing Escherichia coli are u-sually resistant to many different types of antimicrobial drugs.Carbapenems are the first choice to treatment of severe infections of Escherichia coli .
2.The reasons and treatments of recurrent or residual cholesteatoma in middle ears.
Zhaoxin FANG ; Yaodong XU ; Yongkang OU ; Suijun CHEN ; Yiqing ZHENG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2008;22(13):583-584
OBJECTIVE:
To explore the reasons and treatments of recurrent or residual cholesteatoma in middle ears after operations.
METHOD:
The clinical data of 102 cases (105 ears) with recurrent or residual cholesteatoma was retrospectively analyzed.
RESULT:
The main reason of recurrent or residual cholesteatoma is incomplete removal of cholesteatoma in the former operations or obstructive drainage of middle ears after operations. Twenty ears healed through cleaning with otoendoscope. Eighty-five ears underwent the second operations of radical mastoidectomy including 23 tympanoplasty meanwhile. The air-conductive auditory threshold of them all decreased more than 15 dBHL.
CONCLUSION
For the ears with low facial ridges and non-obstructive drainage of mastoid, tympanic antrum and tympanic cavity, its possible to be cured through cleaning with otoendoscope. For those with high facial ridges and obstructive drainage , it's essential to perform the second operations of radical mastoidectomy and some of them are suitable for tympanoplasty meanwhile.
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Child
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Child, Preschool
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Cholesteatoma, Middle Ear
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surgery
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Female
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Humans
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Male
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Middle Aged
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Postoperative Period
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Recurrence
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Retrospective Studies
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Treatment Outcome
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Young Adult
3.Reconstruction and practice of key nodes in the clinical pathway information system
Xinyu CHEN ; Hong SUN ; Qinghong WANG ; Fang XIONG ; Zhaoxin QIAN
Chinese Journal of Hospital Administration 2018;34(4):288-291
Xiangya Hospital of Central South University has reconstructed its key nodes of the clinical pathway information system in order to promote efficient and extensive implementation of the clinical pathway. Its methods include introduction of new classification of clinical pathways, enriched contents of clinical pathway forms, and design of such concepts as "Standard stage date", "Big category of drugs","Grouping prescriptions" and "Concreted mutation".
4.Effects of aconitine poisoning and Shuang-huang-lian treatment on the expression of myocardial ryanodine receptor.
Jinjun LI ; Zhaoxin QIAN ; Xiaozhe XIA ; Limin FANG ; Guilin XIAO
Journal of Central South University(Medical Sciences) 2016;41(3):244-250
OBJECTIVE:
To study the impact of aconitine and Shuang-huang-lian injection on the expression of myocardial ryanodine receptor (RyR2) and to discuss the anti-arrhythmic mechanisms of Shuanghuanglian against aconitine poisoning.
METHODS:
A total of 52 SD rats were randomly divided into 3 groups: aconitine group (group A, n=20), Shuang-huang-lian group (group B, n=20) and control group (group C, n=12). Aconitine at a dose of 0.3 mg/kg was firstly administrated to the rats of group A and group B through gavage. 30 minutes later, normal saline was delivered to rats in group A through intraperitoneal injection, while rats in group B received Shuang-huang-lian at a dose of 100 mg/kg through intraperitoneal injection. Group C accepted twice administration of normal saline by gavage and intraperitoneal injection. The disposals for each group were implemented for 10 consecutive days. The rats' electrocardiograms (ECG) were recorded on day 1, 3, 6 and 10. Immunofluorescent staining technique and real-time quantitative PCR technique was used to detect the protein and mRNA expression of myocardial RyR2.
RESULTS:
The arrhythmia scores of group B at different time were lower than group A and the difference was statistically significant (P<0.05 or P<0.01). The protein and mRNA expression of RyR2 in group A was slightly increased on the first day compared to group B and group C, with no statistical significance (P>0.05). On day 3, 6 and 10, the expression of RyR2 protein and mRNA in group A was dramatically increased compared to group B and group C (P<0.01).
CONCLUSION
Shuang-huang-lian injection can effectively prevent the arrhythmia caused by aconitine, which is related to suppressing the aconitine-induced RyR2 expression.
Aconitine
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Animals
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Anti-Arrhythmia Agents
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Drugs, Chinese Herbal
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therapeutic use
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Rats
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Rats, Sprague-Dawley
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Ryanodine Receptor Calcium Release Channel
5.Inhibition of Microcystis aeruginosa by Bacillus subtilis fmb60 non-ribosome peptide metabolites.
Jie YANG ; Zishan WANG ; Jinlong CHAI ; Yang CHEN ; Jing LU ; Yaowei FANG ; Shujun WANG ; Zhaoxin LU
Chinese Journal of Biotechnology 2021;37(2):625-634
Microcystis aeruginosa, a type of algal bloom microalgae, is widely distributed in water, causing serious deteriorated effects on humans and the ecological environment. As a biocontrol microorganism, Bacillus subtilis can synthesize various bioactive substances through non-ribosomal peptide synthetase, to inhibit the growth of M. aeruginosa. Thus, it is imperative to investigate the non-ribosomal peptide (NRP) metabolites of B. subtilis fmb60. Three NRP metabolites from B. subtilis fmb60 including bacillibactin, surfactin and fengycin were extracted and identified by genome mining technology. The growth inhibition of M. aeruginosa was studied by adding various concentrations of NRP metabolites. The half-effect concentration value (EC50.4 d) of M. aeruginosa was 26.5 mg/L after incubation for 4 days. With the increasing concentration, the inhibitory effects of NRP metabolites of B. subtilis fmb60 on M. aeruginosa was enhanced significantly. Compared with the control group, with the addition of 50 mg/L NRP metabolites to the M. aeruginosa, the content of Fv/Fm, Fv/Fo and Yield parameter after cultured for 4 days were decreased by 2.8%, 1.7% and 2.0%, respectively. Those findings indicate that the NRP metabolites of B. subtilis fmb60 can significantly inhibit the photosynthesis and metabolism of M. aeruginosa, which provides a theoretical foundation for the development of biological algae inhibitor of B. subtilis.
Bacillus subtilis
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Humans
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Microcystis
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Peptides
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Photosynthesis