Objective To compare the resonance acoustic characteristics between 7~16 years old Down syn-drome children and normal children .Methods A total of 29 cases of 7~16 years old Down syndrome children and 90 cases of 7~16 years old normal children pronuanced /a/,/u/,/i/,each sound for 3 times ,every time 1 to 2 sec-onds .The oral resonance acoustic parameters ,including F1 (a) ,F2 (a) ,F1 (i) ,F2 (i) ,F1 (u) ,F2 (u) ,the distance of tongue and the distance of jaw ,were recorded by real analysis .Analysis of variance was used for the voice acous-tic .Results With age ,there was a significant decrease tendency of the value of F2 (a) and a very significant growth tendency of the values of F2 (i) and the distance of tongue in normal children .The value of F2 (i) inrcreased signifi-cantly in Down syndrome children .The values of every oral resonance acoustic parameters were not different signifi-cantly on sex in normal children .The values of F1 (i) and F1 (u) in Down syndrome boys were significantly less than those of in the Down syndrome girls ,but the the distance of jaw in Down syndrome boys were significantly greater than those of in the Down syndrome girls .The values of F2 (u) and F1 (i) in Down syndrome children were signifi-cantly greater than those of in normal children ,but the distance of tongue was significantly less than those of in nor-mal children .Conclusion With age ,in normal children ,the amplitude of the tongue forward movement and the range of tongue back and forward movement were increased significantly ,the oral focus moved forward gradually .In Down syndrome children ,the amplitude of the tongue back movement ,and the range of tongue back and forward movement ,were poor than those of in normal children .The Down syndrome children were easier to suffer from oral resonance disorder than normal children .

Objective:To discuss the effect of cochlear implant and hearing aid to voice quality.Method:Four groups of randomly selected 73 subjects were compared. The groups comprised: cochlear implant children, children using hearing aids, normal hearing children and deaf children with no hearing instrumention. The latter two groups were control groups. Each subject was required to phonate // about 3 s using 'voice assessment' to record the sound, and following voice acoustice variables were analysed: F0,SDF0,Jitter,Shimmer,NNE,HNR,SNR.Result:Voice F0 of children with cochlear implant was significantly lower that the other three groups(P<0.05);there were no significant difference between hearing aid group, normal hearing gourp, and deaf children group with no intervention(P>0.05);SDF0 of cochlear implant group and hearing aids group were significantly larger than the normal hearing group and deaf children group with no intervention(P<0.05),and the interaction of two factors(hearing and sex ) was close to the critical level of significance(P>0.05),which means that cochlear implant and hearing aid could have affected the SDF0 of female children more than those of male children. There were no significant difference among all the four groups about voice quality variables: Jitter,Shimmer,NNE,HNR,SNR.Conclusion:Cochlear implant and hearing aid mainly change the voice F0 and/or SDF0, and have no effect on the voice quality variables.

Objective Rats bladder ncNOS IR following intravesical instillation of capsaicin after chronic spinal cord injury(SCI) was studied to investigate the mechanism of ncNOS IR in pathological bladder. Methods 44 adult Sprague Dawley rats were randomly divided into normal group,sham injury group and spinal cord injury group(4～5 weeks after T 8～9 spinalization).A dose of 1 mmol/L capsaicin was instilled in part of SCI rats.the distribution of ncNOS IR in bladder base,bladder body and bladder dome of all rats were examined. Results In rats,the ncNOS IR did not differ between the normal group (11.1?0.9) and the SCI group (12.8?1.3).Intravesical instillation of capsaicin significantly up regulated ncNOS IR in rat bladder of SCI group (26.1?2.7, P

This study was undertaken to examine ncNOS IR in bladder after spinal cord injury (SCI). In the meantime, we determine rats bladder ncNOS IR following intravesical instillation of capsaicin after SCI. Adult Sprague Dawley rats and guinea pigs were randomly divided into normal group, sham injury group, spinal cord injury group (4～5weeks after T 8～9 transection). A dose of 1mmol/L intravesical capsaicin was instilled in a part of SCI rats. Bladders of all animals were divided into three tissue pieces: bladder base, bladder body and bladder dome,in which the distribution of ncNOS IR was examined. Spinal transection induced a significant ncNOS IR increase in guinea pig bladder base. In rats, the quantity of ncNOS IR did not differ between the two groups. Instillation of intravesical capsaicin can cause significant up regulation of rat bladder ncNOS IR after SCI. The increase of ncNOS IR in SCI animals bladder indicates that NO may play an important role in the regulation of micturition reflex after SCI. In SCI rats, ncNOS IR can significantly be up regulated by intravesical capsaicin instillation, suggesting that NO may act as a factor in the action of capsaicin.

Objective To investigate the acoustic characteristics of the voice of 3~6 years old children with hearing-impairment. Methods The voice samples were collected from 148 hearing-impaired children and 122 normal children, all aged 3~6 years. The acoustic parameters including F0, F0SD, jitter, shimmer, NNE, OQ, SQ, AQ, STF were compared. Results The F0, SQ, STF, F0SD, jitter were more in the children with hearing-impairment than in the normal children (P<0.05). There was no significant difference between hearing-impairment and normal children in shimmer, NNE, OQ and AQ (P>0.05). There was no significant difference of all the acoustic parameters among children with different ages (P>0.05), except the NNE was less in 3~4 years old children than 5~6 years old children (P<0.05). There was no significant difference of all the acoustic parameters between boys and girls (P>0.05). Conclusion The vibration of vocal cords is instable and irregular,saying overquick and close intensely in hearing-impaired children. The age and sex do not influence the acoustic parameters, except NNE is less in younger children.

Objective To explore the speech respiratory characteristics of 7-15 years old children with spastic cerebral palsy and the differences between ordinary children and children with cerebral palsy.Methods The maximum phonation time (MPT) and maximum counting ability (MCA) of 90 ordinary children and 58 children with cerebral palsy aged 7-15 years were measured and a comparison between the 2 groups were conducted.Results (1) Age has significant effects on MPT and MCA of the ordinary children (P ＜0.05).The MPT and MCA of the ordinary children at the age of 7 ～9 years and 10 ～ 12 years are lower than the ones at the age of 13 ～ 15 years.Age has almost no effects on MPT and MCA of children with cerebral palsy(P ＞0.05) ; (2)Sex has no significant effects on MPT and MCA of ordinary children and children with cerebral palsy (P ＞0.05) ; (3) The MPT and MCA of children with cerebral palsy are significantly lower than those of ordinary children (P ＜0.05).Conclusions The MPT and MCA of ordinary children are increased with the growth of age,especially after the age of 13 years,but the children with spastic cerebral palsy do not show that kind of trend and demonstrate a state of retardation of speech respiratory function.Compared with ordinary children,the children with spastic cerebral palsy are easier to suffer from insufficient respiratory support and poor respiratory-phonatory coordination.

Objective This study aimed to develop reading material for nasalance evaluation ,and find out na-salance scores associated with genders .Methods The NasalView ? (Tiger Electronics Inc .,Seattle ,USA) was used to obtain nasalance values in the new and old material .A total of 102 subjects (51 males ,51 females) who have lived in Beijing for 18 years or longer at the time of the experiment received the tests ,the results from two pieces of material and the mean nasalance gender score differences were compared .Results The mean nasalance scores of sen-tences were 56 .58% ± 3 .43% for'Nasal sentence',33 .86% ± 5 .24% for'Oral sentence',and 49 .49% ± 4 .13% for'Oro -nasal sentence'respectively in the new material .The mean nasalance scores of sentences were 54 .31% ± 4 .43% for'Nasal sentence',35 .64% ± 5 .90% for'Oral sentence',and 47 .12% ± 4 .96% for'Oro-nasal sentence're-spectively in the old material .There were significant differences between males and females through materials .The nasalance gender score differences showed that females were bigger than males (P<0 .01) .This study found a cor-relation between the new and old materials :'Nasal sentence'(r=0 .899 ,P< 0 .01) ,'Oral sentence'(r= 0 .850 ,P<0 .01) ,and'Oro -nasal sentence'(r=0 .851 ,P<0 .01) .The standard error difference showed that the new was smaller than the old (P<0 .01) .Conclusion The new material for nasalance evaluation has a better test validity ,and there is a high correlation between the old materials .The usage of the new material for nasalance evaluation will produce more accurate results with higher credibility .Normal female's nasalance scores were higher than males .

Objective To evaluate laparoscopic D2 lymph node dissection gastrectomy in the treatment of advanced gastric cancer.Methods The clinical data of 239 cases of advanced gastric cancer admitted from January 2004 to June 2011 were respectively analyzed,patients were divided into laparoscopic resection group and open surgery group.Data analysis was performed by SPSS 19.0 statistical software.Results There were 102 cases in laparoscopic group,and 137 cases in open group.The length of incision,operative blood loss,recovery time of gastrointestinal function,food-taking time and postoperative hospital stay in laparoscopic operation group were (5.0 ± 1.1) cm,(70 ± 44) ml,(57 ± 14) h,(68 ± 12) h,(7.1 ± 1.4) d and in open operation group were (17.4 ± 2.1) cm,(107 ± 59) ml,(75 ± 12) h,(91 ±15) h,(9.9 ± 1.8) d respectively.There were significant differences between the two groups (t =-58.86,-5.50,-10.72,-12.58,-12.58,all P =0.00).There was no significant differences between the two groups in operative time (t =1.63,P =0.11),with operative time in laparoscopic operation group of (192 ± 32) min,and (185 ± 30) min in open group.Average proximal,distal cutting edge and the average number of lymph node harvested were (5.0 ± 1.0) cm,(4.7 ± 0.8) cm,(27.6 ± 7.2) in laparoscopic operation group,and (5.1 ±0.9) cm,(4.7 ±0.9) cm,(27.0 ±6.5) in open group (t =-0.61,0.10,0.68,P ＞ 0.05).The 3-,5-,7 d white blood cell counts in laparoscopic group was (11.1 ± 1.3) ×109/L,(9.5 ± 1.4) × 109/L,(7.0 ± 1.5) × 109/L,and (12.8 ± 1.3) × 109/L,(11.1 ± 1.5) × 109/L,(8.6 ± 1.3) × 109/L,in open group (t =-9.83,-8.88,-9.40,all P =0.00).Complications developed in 9.8 ％ (10/102) in laparoscopic operation group,and 17.5 ％ (24/137) in open group (x2 =0.285,P =0.09).The 1-year,3-year,5-year survival rate of patients in laparoscopic group were 96.1％,74.1％,47.2％,and 95.6％,70.0％,50.9％ in open group (x2=0,0.04,0.21,P ＞0.05).Conclusions In selected cases,laparoscopic D2 lymph node dissection gastrectomy for advanced gastric cancer is safe and effective,and long-term outcomes are satisfactory.

Objective To investigate the difference and the correlation of the vowel's phonation threshold pressure and nasalance in normal subjects .Methods The Aeroview (GLOTTAL Enterprises)with the materials /pa/,/pi/,/pu/ was used to obtain phonation threshold pressure values and The NasalviewTM (Tiger Electronics Inc .,Seattle ,Shanghai) with the materials /a/,/i/,/u/was used to obtain nasalance values .A total of 30 subjects (15 males ,15 females ,and aged 19~30) years old received the tests .The results of the vowel's phonation threshold pressure and nasalance gender score difference were compared .Results The PTP gender scores of /a/,/i/,/u/in maleswere4.53±0.70cm,5.21±0.80cm,5.87±1.45cm,andinfemaleswere4.09±0.30cm,4.47±0.30cm, and 4 .77 ± 0 .32 cm ,respectively .There were significant differences between males and females in PTP values (P<0 .05) .The PTP gender score differences showed that males were greater than that of females .There were signifi‐cant differences in different vowels (P<0 .05) .The nasalance gender scores of /a/,/i/,/u/in males were 30 .2 ± 5 . 75% ,38 .8 ± 8 .54% ,26 .5 ± 6 .03% ,and in females w ere32 .4 ± 7 .14% ,40 .5 ± 11 .8% ,and 30 .1 ± 4 .83% ,respec‐tively .There were no significant differences between males and females in nasalance values (P> 0 .05) .The na‐salance gender score differences showed that females were greater than males .There were significant differences in different vowels(P<0 .05) .This study found a weak correlation between PTP and nasalance .Conclusion The relationship between oral pressure and nasal flow cannot be accurately measured by measuring PTP ,and /pa/is a relatively suit‐able material for testing oral pressure .

Objective To study the characteristics and difference of speech rate in monologue and reading in normal adults .Methods Through the acquisition of spontaneous speech and reading from 30 normal adults ,the syl‐lables per minute (SPM ) and the words per minute (WPM )were separately calculated .The monologue materials in‐clude ten topics :self introduction hobby ,hometown and so on;the reading material was the stars in the sky written by Ba Jin .We also analyzed whether there was a significant difference in genders and types of expression for speech rates .Results With the expression of monologue :SPM (male)=226 .13 ± 25 .97 ,SPM (female)=198 .73 ± 40 .With the expression of reading :SPM (male)= 208 .93 .13 ± 29 .35 ,SPM (female)= 220 .53 .± 26 .52 ,WPM (male)=104 .67 ± 13 .43 ,WPM (female)=111 .80 ± 12 .73 .Pearson correlation coefficient of SPM and WPM had reached 0 .926(r=0 .926) in the spontaneous speech and 0 .969 (r=0 .969) in reading .There were no significant differences in both genders (P=0 .42) and types of expression(P=0 .72) .The interaction effect of genders and types of ex‐pression was significant (P=0 .006) .For females ,the rate of reading was significantly higher than monologue (P=0 .025);for monologue ,there was a significant difference between males and females(P=0 .035) .The male was higher than the female in the speed of monologue .Conclusion The statistics revealed a high correlation between SPM and WPM .Genders and types of expression did not play an important role on the speech rate .The interactioneffect of gender and type of expression was significantly high (P=0 .006) .