Objective To investigate the potential effects of simvastatin on angiotensin Ⅱ-stimulated secretion and proliferation of adrenocortical carcinoma H295R cells.Methods The H295R cells were divided into control group, Angiotensin Ⅱgroup, simvastatin group and Angiotensin Ⅱ plus simvastatin group.Cortisol in medium was determined by chemiluminescent method , and aldosterone was determined by radioimmunoassay .The mRNA expression of 11 beta-hydroxylase ( CYP11B1 ) and aldosterone synthase ( CYP11B2 ) were examined by RT-qPCR.Cell proliferation was detected by MTS method.Results Compared with control group, angiotensin Ⅱincreased the secretion of cortisol and aldosterone, and the expression of CYP11B1 and CYP11B2.Simvastatin decreased cortisol secretion and CYP11B1 mRNA expression ( P<0.05 ) .Simvastatin also inhibited angiotensinⅡ-induced the secretion of cortisol and aldosterone , and the expression of CYP 11 B1 and CYP11 B2 compared with Angiotensin Ⅱgroup ( P<0.05 ) .Angiotensin Ⅱhad no effect on cell proliferation , while simvastatin significantly inhibited cell proliferation .The inhibitory effect of simvastatin on proliferation was enhanced when simvastatin was prescribed with angiotensin Ⅱ( P<0.05 ) .Conclusions Simvastatin inhibits angiotensin Ⅱ-induced secretion of cortisol and aldosterone in H295R cells.Simvastatin inhibits cell proliferation, which could be enhanced by angio-tensin Ⅱ.

Objective To investigate the effect of plateau training on military operation ability ,evaluate the real situa-tion of combat forces on the plateau , and to provide data for effectively improving the combat effectiveness of the army stationed on the plateau .Methods According to the standards , the levels of high altitude acclimatization , physical work capacity (VO2max, PWC170) and mental work capacity (digit span, digit symbol, pursuit aiming and visual reaction time) were measured to assess the military operation ability of plateau training troops , including troops that entered the plateau from the plain(plateau-entering troops), troops stationed on the plateau (plateau troops) and troops stationed in the plain ( plain troops ) .Results The troops on the plateau all reached basic acclimatization .The aerobic capacities of these three types of troops were of the standard medium level , and the maximum oxygen uptake ( VO2max ) of the plain troops was greater than that of that of plateau-entering troops(P<0.01), and the VO2max of the plateau troops was greater than that of plateau-entering troops(P<0.01).Compared with the plain troops, the VO2 max and PWC170 of the plateau-entering troops decreased by 25.7%and 27.7%respectively.There was no significant difference in the digit span , digit symbol, pursuit aiming between these 3 kinds of troops , but the visual response of the plateau-entering troops was prolonged ( P<0.01), while the pursuit aiming of the plateau troops was lower than that of the plateau-entering troops(P <0.05). Conclusion Acclimatization-promoting measures and plateau training can contribute to high altitude acclimatization formation, but cannot overcome the objective adverse effects of the plateau on physical fitness .Two months of plateau training fails to improve physical fitness.Therefore, special attention should be paid to the research on scientific and effective measures to improve physical fitness on the plateau .

Objective:To explore the effect of Hejie Jusan Decoction on pyroptosis in diabetic nephropathies (DN) rats; To explore its mechanism based on the NF-κB/nucleotide binding oligomeric domain like receptor protein 3 (NLRP3)/Caspase-1 signal pathway.Methods:Totally 60 SD rats were divided into blank group, model group, potassium losartan group, and Hejie Jusan Decoction high-, medium-, and low-dosage groups. In addition to the blank group, the other five groups were established DN rat models. Hejie Jusan Decoction high-, medium-, and low-dosage groups were given 0.5, 1.0, and 2.0 g/ml concentrations of Hejie Jusan Decoction, respectively; the potassium losartan group was orally administered with 20 mg/kg potassium losartan; The blank group and model group were given equal volumes of physiological saline by gavage, once a day, for consecutive 28 days. After medication, the levels of serum creatinine (SCr), urea nitrogen (BUN), and 24-hour urine protein (24 hUP) were detected; pathological changes in renal tissue were detected by HE staining; Western blot method was used to detect the expressions of P65, NLRP3, Caspase-1, and GSDMD protein in renal tissues.Results:Compared with the model group, the SCr, BUN, and 24-hUP of rats in the Hejie Jusan Decoction high-dosage group and the potassium losartan group decreased ( P<0.01). The expressions of P65, NLRP3, Caspase-1, and GSDMD protein in renal tissues of Hejie Jusan Decoction groups and potassium losartan group decreased ( P<0.05 or P<0.01). Conclusion:Hejie Jusan Decotion may affect pyroptosis by inhibiting the activation of NF-κB/NLRP3/Caspase-1 pathway, reducing renal function damage and pathological changes, so as to achieve the effect of treating diabetes nephropathy.

Objective To investigate the value of F-actin autoantibodies in the serum of patients with systemic lupus erythematosus (SLE),and to explore the relationships between F-actin autoantibodies and other clinical indicators.Methods ELISA was established to detect serum levels of F-actin autoantibodies in 93 inpatients with SLE from March 2017 to January 2018 (case group,n=93),72 patients with rheumatoid arthritis (RA) (disease control group) and 83 healthy subjects (healthy control group) were included during the same period.The positive rates of F-actin autoantibodies between the case group and the two control group were compared.Clinical data including SLE disease activity index (SLEDAI),immuno-globulin (lg)G,erythrocyte sedimentation rate (ESR),anti-dsDNA,and antinuclear antibody (ANA) of 93 patients with SLE were collected and the correlation analysis between F-actin autoantibodies units was applied respectively.The diagnostic performance of F-actin autoantibodies in SLE was analyzed by using the receiver operating characteristic curve (ROC).T test,Chi-square test and Spearman/Pearson correlation analysis were applied for statistical analysis.Results The serum levels of F-actin autoantibodies in the SLE case group,disease control group,and healthy control group were (18±13),(12±6),and (11±5) U,respectively,the differences between SLE case group and disease control group,and healthy control group were significant (t=3.163,P=0.001 9;t=4.436,P＜0.01).The positive rates of F-actin autoantibodies were 33％(31/93) in patients with SLE,10％(7/72) in disease control group,and 4％(3/83) in healthy control group.The F-actin autoanti-bodies units in SLE were correlated with SLEDAI,IgG,ESR,anti-dsDNA,and ANA (r=0.273 7,P=0.008 3;r=0.558 7,P＜0.01;r=0.419 9,P=0.000 1,r=0.351 4,P=0.001 1,r=0.460 9,P＜0.01),in which F-actin autoantibodies units showed significant correlation with IgG and ANA.In the ROC curve,the area under the curve(AUC) was 0.62 [95％CI(0.54,0.70)],P=0.001 3.which was statistically significant.When the cut-off value of the F-actin autoantibodies was 14.04 U,the Youden's index (YI) was the largest (YI=0.30),and the sen-sitivity for the diagnosis of SLE was 0.77,the specificity was 0.53.Conclusion The positive rate of F-actin autoantibodies in the serum of patients with SLE is higher than that of RA and healthy controls,so it has certain diagnostic value for SLE.The F-actin autoantibodies units is correlated with both SLEDAI,ESR,and anti-dsDNA,suggesting that F-actin autoantibodies units may be a new biomarker for disease activity assessment of SLE patients.

ObjectiveTo explore the impact of different types of virtual reality environments balance exercise on human balance and posture control. MethodsFrom March to April, 2022, 30 male students from Dalian University of Technology were recruited to accept VR balance training of dynamic background (dynamic VR training) and static background (static VR training), respectively, with an interval of one week. They were measured the average moving speed of the center of pressure of human body under eight conditions before and after training, namely open-eye floor standing, close-eye floor standing, static VR background floor standing, dynamic VR background floor standing, open-eye sponge pad standing, close-eye sponge pad standing, static VR background sponge pad standing, and dynamic VR background sponge pad standing. Meanwhile, the weight of human body sensation was calculated. ResultsBefore training, the average movement speed of center of pressure was higher under the static VR than under open-eye (|t| > 2.811, P < 0.01), and lower than under close-eye (t > 3.279, P < 0.01) on both planes; while it was higher under dynamic VR than under close-eye (|t| > 4.830, P < 0.001). After dynamic VR training, the average movement speed of center of pressure increased under open-eye stable floor standing (t = 2.305, P < 0.05), decreased under close-eye on both planes (t > 3.405, P < 0.01), and decreased under static and dynamic VR on both planes (|t| > 3.285, P < 0.01). After static VR training, the average movement speed of center of pressure increased under open-eye floor standing (t = 2.224, P < 0.05), decreased under close-eye sponge pad standing (t = 2.223, P < 0.05), and decreased under dynamic VR on both planes (|t| > 2.380, P < 0.05). The weight of vision decreased after training (t > 4.132, P < 0.001), and the visual weight under normal proprioception was less after dynamic VR training than after static VR training (t = 3.611, P < 0.01). ConclusionUnder static VR background, the balance stability is poorer than under open-eye, but stronger than under close-eye. Under dynamic VR background, the balance stability is poorer under close-eye. VR balance training may decrease the stability without interference, but improve the stability under interference, which may result from reducing the dependence on visual sensation and strengthening the use of vestibular sensation, especially after VR training with dynamic background.

Objective:To analyze the efficacy of neuronavigation‐guided pure endoscopic endonasal transsphenoidal approach for the surgical removal of complex pituitary adenomas .Methods :The clinical data of 60 patients who received pure endoscopic endonasal transsphenoidal surgery for complex pituitary adenomas from Apr 2010 to Sep 2012 was retrospectively analyzed . These patients had one or more characteristics which including tumors more than 4 cm in size(34 patients) ,Knosp grade 3‐4(41 patients) ,recurrent or residual tumor(17 patients) .All patients received pure endoscopic endonasal transsphenoidal surgery and neuronavigation was used . Neuro‐ophthalmological , neuroimaging and endocrinological examination were performed postoperatively .Results:There were 33 nonfunctional adenomas and 27 functional adenomas .Overall ,the tumors were gross totally removed in 42 patients ,subtotal removed in 11 patients and partially removed in 7 patients .Postoperative visual acuity was improved in 32 patients ,endocrine remission was achieved in 8 patients .The gross total removal was achieved in 18 ,23 and 12 patients for tumors more than 4 cm in size ,Knosp grade 3‐4 adenomas ,recurrent or residual adenomas ,respectively .The postoperative complications included electrolyte disturbances in 4 patients ,cerebral spinal fluid leakage in 3 patients ,insipidus in 1 patient .And 1 patient had visual impairment ,hypopituitarism and hypersomnia caused by postoperative hemorrhage .And 5 patients had reoperation .Conclusions :Neuronavigation‐guided pure endoscopic endonasal transsphenoidal surgery is safe and effective for complex pituitary adenoma .The effectiveness of that approach for invasive pituitary adenomas (Knosp grade 4) and complex functional adenomas still need to be promoted .

Objective:To analyze the mechanism of Jindan Tablets, Xiaoyan Lidan Tablets and ursodeoxycholic acid in the treatment of gallstone and cholecystitis based on network pharmacology; To conduct a comparative analysis.Methods:The chemical components of Jindan Tablets, Xiaoyan Lidan Tablets and ursodeoxycholic acid and their drug targets were collected from Traditional Chinese Medicine Database and Analysis Platform (TCMSP). DAVID 6.8 database was used to search for the associated diseases of the drug targets. The disease targets of gallstone and cholecystitis were collected from GeneCards and other databases. The protein-protein interactions network was established based on the intersecting targets of three drugs and two diseases. KEGG enrichment analysis was performed based on the DAVID 6.8 database. Cytoscape 3.7.1 software was used to construct a complex network and topology analysis of component- target- disease between three drugs and diseases.Results:222 chemical components and 3 133 drug targets were collected for Jindan Tablets. 104 chemical components and 1 425 action targets were collected for Xiaoyan Lidan Tablets. 1 chemical component and 119 action targets were collected for ursodeoxycholic acid. The three drugs were associated with 31 diseases. 1 382 disease targets for gallstones and cholecystitis were collected. There were 237, 163 and 33 targets for gallstones and cholecystitis in the three drugs, of which 17 were shared by the three drugs and 20 were shared by Jindan Tablets and Xiaoyan Lidan Tablets. Based on the DAVID database, 113, 74 and 10 significant KEGG enrichment pathways were obtained for the three drugs respectively.Conclusions:The three drugs shared many targets and pathways in the treatment of gallstones and cholecystitis, which all had the function of regulating metabolism and inhibiting inflammatory response, while participating in apoptosis, oxidative stress and cancer pathology process. However, they had their own special effects, with Jindan Tablets favoring involving in the cancer process and inhibition of inflammation, and promoting angiogenesis. Xiaoyan Lidan Tablets and ursodeoxycholic acid focused on regulating cholesterol metabolism, and Xiaoyan Lidan Tablets also regulated steroid metabolism and inhibit inflammation, while ursodeoxycholic acid regulated bile acid metabolism.