Objective To investigate the role of nine respiratory pathogens (hereinafter referred to as United Nine Detection(UND)) in acute exacerbation of interstitial lung disease. to provide an evidence of the pathogenesis and effective diagnosis and treatment of AE-ILD. Methods We detected the expression of of IgM antibody of UND in respiratory infection pathogens in serum in the AE-ILD group,the ILD stable group and the normal control group,and observed their dynamic changes. Results The test results of UND showed that the Pathogen-positive rate were 52.1%,27.8%,22%,in 73 cases of the AE-ILD group,72 cases of the ILD stable group and 50 cases of the normal controls,respectively. Statistical analysis showed that pathogen infection rate has statistics difference between the AE-ILD group and the ILD stable group,between the AE-ILD group and the normal control group respectively(P=0.003;P=0.001),while the ILD stable group and the normal control group hadd no significant difference (P = 0.417). The virus positive rates of the three groups were 31.5%,16.7%,10%respectively. Statistical analysis showed that the virus infection rate had statistics difference between the AE-ILD groups and the ILD stable group,between the AE-ILD group and the normal control group(P=0.037;P=0.005). Mycoplasma,influenza B virus,respiratory syncytial virus had high positive rate in these three groups. Dynamic changes of the IgM antibody showed that the positive number of pathogens decreased gradually in long admission of patients with AE-ILD. Conclusions The infection rate of UND in patients with AE-ILD is higher than patients with ILD in the stable stage and the normal controls. It suggests that AE-ILD may be associated with infection.

Objective:To exploring the uptake of fibroblast activation protein (FAP) inhibitor (FAPI) in pancreatic cancer through 68Ga-FAPI-04 PET/CT imaging, and provide a basis for the FAP-targeted imaging of pancreatic cancer. Methods:Pancreatic cancer-patient-derived tumor xenograft (PDX) mouse models ( n=8) were developed, then 68Ga-FAPI-04 and 18F-FDG microPET/CT imaging were performed (4 in each group). The differences of percentage activity of injection dose per gram of tissue (%ID/g) of 68Ga-FAPI-04 and 18F-FDG were analyzed by independent-sample t test. 68Ga-FAPI-04 and 18F-FDG PET/CT imaging were performed in 5 patients (4 males, 1 female, age: 46-74 (63.0±11.9) years) with pancreatic cancer, and the maximum standardized uptake value (SUV max) of 68Ga-FAPI-04 and 18F-FDG in primary pancreatic cancer and the SUV max ratio of liver metastases to liver tissue were compared by paired t test. Results:MicroPET/CT imaging showed that 68Ga-FAPI-04 was obviously uptaken at all time points in the tumor of PDX mice. The uptake of 68Ga-FAPI-04 in PDX mice 60 min after injection was significantly higher than that of 18F-FDG ((6.58±0.44) and (4.29±0.13) %ID/g; t=4.152, P=0.008 9). PET/CT showed that the SUV max of 68Ga-FAPI-04 in pancreatic cancer was significantly higher than that of 18F-FDG (16.82±3.08 and 5.14±2.20; t=6.893, P=0.000 1) and the SUV max ratio of liver metastases to liver tissue of 68Ga-FAPI-04 was also significantly higher than that of 18F-FDG (4.57±1.47 and 1.30±0.16; t=3.803, P=0.019 1). Conclusion:68Ga-FAPI-04 can be highly uptaken in pancreatic cancer, suggesting that FAP can be a potential target for PET/CT imaging of pancreatic cancer.