1.The Effect of Lysophosphatidic Acid on the Permeability of Blood-Brain Barrier
International Journal of Cerebrovascular Diseases 2006;0(04):-
Lysophosphatidic acid is a intermediary metabolite in lipid metabolism, mainly produced from activated platelets. It may cause the apoptosis of hippocampal neurons, abnormal phosphorylation of tau protein and increased permeability of the tight junction of endothelial cells, which is closely correlated with pathological changes of ischemic cerebrovascular diseases, Alzheimer's disease and vascular dementia. It is shown that lysophosphatidic acid may induce the opening of the blood-brain barrier under pathological conditions, and enters central nervous system through the blood-brain barrier, and causes different effects of pathological changes by acting on various cells.
2.Influence of tetramethylpyrazine on oxygen metabolism during the respi ratory burst of human neutrophiis
Zhaohui ZHANG ; Shaozu YU ; Baolu ZHAO
Chinese Journal of Clinical Pharmacology and Therapeutics 2001;6(1):8-11
AimTo investigate the influence of tetramethylpyrazine(ligustrazine, Lig) on oxygen consumption and superoxide during the respiratory burst of human neutrophils. MethodsIt was observed by using ESR spin trapping, spin probe oxymetry and luminol-dependent chemiluminesence(CL) . Results Lig had no influence on oxygen consumption during the respiratory burst of neutrophils(P>0.05), but had remarkable inhibition on CL response generated by neutrophils(P<0.01), and had scavenging effect on O2 and OH ·generated by neutrophils, which were demonstrated in xanthine/ xanthine oxidase system and Fentons reaction(P<0.01) . ConclusionLig has no inhibiting effect on oxygen metabolic function of neutrophils,but protects tissue from injury caused by activated neutrophils through scavenging oxygen radicals.
3.Clinical analysis of primary hyperaldosteronism and predictive value of preoperative test for primary hyperaldosteronism
Xiaojing YU ; Zhaohui LV ; Xiaomei WANG
Chinese Journal of Postgraduates of Medicine 2006;0(10):-
Objective To evaluate the clinical feature of the aldosterone-producing adenoma(APA) and idiopathic hyperaldosteronism(IHA)in primary hyperaldosteronism.Methods Retrospectively analyzed clinical feaures of 76 patients with APA and 17 patients with IHA.Results (1)Compared with the patients with IHA,the patients with APA had higher plasma and urinary aldosterone,lower serum potassium.(2)Furosemide provocation test(FPT)in 48 patients with APA and 14 patients with IHA were carried out.It was found that the plasma aldosterone after FPT was increased or no changed in 27 patients with adenoma,and increased in the others.It was increased in all IHA patients.(3)The diagnostic accuracy rate of CT was 84.2% in 88 patients with computed tomography imaging(CT).Conclusion The anomalies of laboratory in patients with APA are more evidence than those of IHA.The results of FPT are overlapped in a considerable amount of APA and IHA.Now those with elevation of serum aldosterone after the FPT can not exclude the adenoma.The diagnostic accuracy rate can be improved according to FPT and CT.
4.Expression of transient receptor potential M8 in human knee joint cartilage
Bin YU ; Boyuan NIE ; Zhaohui YANG
Chinese Journal of Tissue Engineering Research 2014;(20):3117-3121
BACKGROUND:Transient receptor potential protein channel is distributed in many kinds of tissue and cells in human body, and takes part in many physiologic functions and pathological changes of human body. However, up to now, transient receptor potential melastatin 8 (TRPM8) has not been reported in human cartilage.
OBJECTIVE:To study the expression of TRPM8, a subfamily member of transient receptor potential gene, in human knee joint cartilage.
METHODS:Fifteen samples were selected from patients after amputatioin due to severe trauma. Total protein was extracted from 300 mg cartilage tissue of every patient. To detect the distribution of TRPM8 in human
cartilage, cartilage sections were col ected in the same region. The expression and distribution of TRPM8 in human cartilage was detected by western blot analysis and immunofluorescence respectively.
RESULTS AND CONCLUSION:Western blot analysis showed that specific TRPM8 band was visible at molecular weight of 130 000, and as the age increased, TRPM8 expression in human cartilage was gradual y decreased. Immunofluorescence staining results showed that TRPM8 was evenly located in the cellmembrane of chondrocytes. TRPM8 is expressed in the human knee cartilage, specifical y in member of chondrocytes.
5.Identificantion of Common Pathogenic Candida Species by PCR-RFLP Analysis
Jin YU ; Zhaohui JI ; Ruoyu LI
Chinese Journal of Dermatology 1994;0(02):-
Objective To study the molecular identification method of Candida species and lay the foundation of molecular diagnosis for deep candidiasis. Methods PCR was used to amplify the internal transcribed spacer region of ribosomal DNA from 11 type strains of 9 pathogenic Candida spp. and 39 clinical Candida isolates, and the products were digested with three types of restriction endonucleases (MspⅠ,DdeⅠand HaeⅢ). Results Five patterns of products with different molecular weights were obtained after amplification from 9 Candida spp., and the products were digested with MspⅠ, DdeⅠand HaeⅢ, producing 8, 5 and 4 unique band patterns, respectively. Conclusion PCR-RFLP is reliable, specific and useful for the identification of common pathogenic Candida species.
6.Practical digitalized patient records control
Feng HUANG ; Zhaohui YU ; Jian YANG
Chinese Journal of Hospital Administration 1996;0(10):-
Objective The objective of digitalized patient records control is to meet some new demands set on patient records control under the new circumstances. Methods A computer system for digitalized patient records control and supporting processing, management and applications setups were established. Results The system put the contents of the patient records into various categories with difierent standards and designed relatively strict means of authorization. It rendered patient records easier to use and their control more strict, making it possible to make general use of the records without access to the original documents. As a result, data loss was avoided, authority over the data was put under control, data sharing was provided, and full and convenient use of data was realized. Conclusion Digitalized patient records control, a form of shaping computer-based patient records before the latter fully comes into being, enhances the efficiency of patient records application and reduces the pressure of paper records storage. It is a practical and effective way of controlling patient records.
7.The effect of mental nursing intervention on gastric cancer with liver metastasis patients treated with TACE sequential chemotherapy
Ruo ZHANG ; Huiqian YU ; Yiman JIN ; Yaojun YU ; Zhaohui WU
Chinese Journal of Primary Medicine and Pharmacy 2016;23(11):1615-1619
Objective To explore the effect of mental nursing intervention on gastric cancer with liver metastasis patients treated with TACE sequential chemotherapy.Methods Sixty four gastric cancer patients with liver metastasis,no hopes of accepting operations,were included.All patients were divided into control group (32 cases) and observation group (32 cases).The former received routine nursing care and the later received extra mental nursing intervention.The anxiety,depression and quality of life in both groups were evaluated and compared.Results After extra mental nursing intervention,the SDS and SAS scores of the observation group [(41.6 ± 1.8) points and (41.6 ± 2.3) points] were significantly lower than those of the control group [(50.9 ± 1.9) points and (53.0 ± 3.5) points,P =0.040,P =0.036,respectively].The quality of life scores,including the physiological function,body pain,general self rating,vitality,social function,emotional function and mental health score were (45.26 ± 38.26)points vs (35.17 ± 33.65) points (P =0.044),(71.98 ± 17.36) points vs (63.01 ± 18.41) points (P =0.039),(55.59 ± 12.78) points vs (49.67 ± 12.43) points (P =0.048),(74.82 ± 14.23) points vs (55.61 ± 10.58) points (P =0.027),(75.47 ± 12.15) points vs (57.22 ± 14.78) points (P =0.031),(70.24 ± 22.00) points vs (45.92 ± 34.76) points (P =0.004),(78.31 ± 19.11) points vs (67.23 ± 11.96) points (P =0.037),respectively,the scores in the observation group were significantly higher than those in the control group (all P < 0.05).Conclusion The extra mental nursing intervention on gastric cancer with liver metastasis patients,treated with TACE sequential chemotherapy,can not reduce the patients'negative mood,improve the patients'compliance with chemotherapy and the confidence to treatment,but improve the quality of patients'life effectively.
8.Influence of laparoscopic manoeuver on intraabdominal homeostasis
Yuzhong QI ; Sanyuan HU ; Zhaohui SUN ; Wenbin YU ; Hualong YU
Chinese Journal of General Surgery 1997;0(04):-
ObjectiveTo evaluate the influence of laparoscopic operation on pH value, phagocytic function of neutrophil, immunoadhesion activity of RBC CR 1 (complement receptor typeⅠ) in abdominal cavity. Methods Determination of the pH value, phagocytic function of neutrophil, immunoadhesion of RBC CR 1 in abdominal exudatives during laparoscopic procedures was undertaken and results were compared with the relative indexes detected in peripheral vein blood. Results The average pH value was 7.41, the percentage of the phagocyte was 74.6%, phagocytic index 55.7, and immunoadhesion activity of RBC CR 1 was 58% in blood during laparoscopic operation. While the average pH value was 7.14, the percentage of the phagocyte 65.6%, phagocytic index 47.6,and immunoadhesion activity of RBC CR 1 50% in the abdominal exudatives. Conclusion Compared with the peripheral vein blood indexes, the intraabdominal pH value, phagocytic function of neutrophil and immunoadhesion activity of RBC CR 1 decreased significantly during laparoscopic operation. The intraabdominal inflammation may diffuse with the effect of CO 2 pneumoperitoneum when patients with serious inflammation receive laparoscopic operation.
9.Influence of amoxicillin and clavulanate potassium on postoperative infection and inflammatory indexes in emergency intestinal obstruction operation by relaxation suture
Yongyang YU ; Yun HUANG ; Zhaohui LIU ; Fuxing ZHANG
Chinese Journal of Biochemical Pharmaceutics 2016;36(11):53-55
Objective To study the influence situation of amoxicillin and clavulanate potassium for the postoperative infection and inflammatory indexes of patients with emergency intestinal obstruction operation by relaxation suture .Methods 80 patients with emergency intestinal obstruction operation by relaxation suture in department of general gurgery of our hospital from April 2014 to April 2016 were randomly divided into control group and observation group with 40 cases in each group, the control group were treated with routine treatment, the observation group were treated with amoxicillin and clavulanate potassium on the treatment of control group, then the postoperative infection rates, inflammatory related indexes before and after the treatment of two groups were compared.Results The postoperative infection rate of observation group was 5.00%,and it was obviously lower than the 17.50% of control group, the difference was significant(P<0.05); the serum inflammatory related indexes of two groups before the treatment were compared,the differences were not significant ;the serum anti-inflammatory indexes interleukin-4(IL-4), IL-10, IL-13 and pro-inflammatory indexes tumor necrosis factor-α(TNF-α), IL-6 at 1st, 2nd and 5th day after the treatment were all lower than those of control group,the pro-inflammatory index IL-2 were higher than those of control group, the differences were all significant(P<0.05).Conclusion The amoxicillin and clavulanate potassium can reduce the postoperative infection of patients with emergency intestinal obstruction operation by relaxation suture , and its control role for the related inflammatory indexes is better, so its application value is higher and is worthy of clinical promotion.
10.Problems and countermeasures of teaching reform of neurology in CPPT curriculum
Ying YU ; Zhaohui ZHANG ; Jun QIN ; Suping HU
Chinese Journal of Medical Education Research 2016;15(11):1136-1139
In recent years, there has been a comprehensive reform of higher medical education in the Medicine School of Wuhan University. According to the need for reform, the teaching of neurology has to be changed from the traditional form to a new form, and be integrated into the clinical pathophysiology and therapeutics (CPPT) courses. Currently neurology in CPPT takes the form of theoretical lectures, case discussions, combined with practical lessons to observe sections under the microscope and clinical practice, for the cultivation of students'!self-learning ability and clinical thinking. In the commissioning process, it exposes some problems in teaching process due to the characteristics of the course in neurology. For example, the knowledge of neuroanatomy is insufficient and review lessons relatively too short, and the teaching effect may be worse due to the fact that teachers have busy clinic work. In addition, students participate in case discussions with less enthusiasm. To solve these problems, we take some measures to promote teaching reform in neurology, such as increasing the review hours of neuroanatomy section in the CPPT neurology, training a group of specialized medical teachers to enrich and stabilize teacher team, adjusting the content and form of discussion class to improve students'!interest and participation, and increasing assistant jobs by the student to assist discussion teaching.