Objective To investigate 25S rDNA genotype distribution of vaginal Candida albicans and its relationship with antifungal susceptibility. Methods A total of 954 isolates of Candida albicans were subjected to genotype analysis according to the presence and the size of transposable groupⅠintron in 25S rDNA with PCR; 156 isolates were enrolled to detect their antifungal susceptibility of 5 antifungal agents with the M27-A3 broth microdilution method. Results Totally 876 isolates were determined to be intronless genotype A (91.8%,876/954); 58 (6.1%, 58/954) and 20 isolates (2.1%, 20/954) were intron-containing genotype B and genotype C, respectively. The minimal inhibitory concentrations (MIC) of itraconazole and fluconazole against the intron-containing group were significantly higher than those of the intronless group (0.25 versus 0.125μg/ml, P<0.05;0.25 versus 0.125μg/ml, P<0.01), whereas the MIC of nystatin against the intron-containing group was lower than that of the intronless group (4 versus 8μg/ml, P<0.01). The resistance rate of Candida albicans to itraconazole of the intron-containing group was significantly higher than that of the intronless group [24% (19/78) versus 3% (2/78), P<0.01]. Cross-resistance analysis revealed 5 of the 21 (24%) itraconazole resistant isolates were cross-resistant to fluconazole ,and 5 of the 6 (5/6) fluconazole-resistant strains were cross-resistant to itraconazole. Besides, all the five strains simultaneously resistant to itraconazole and fluconazole belonged to the intron-containing group. Conclusion The presence of the transposable group Ⅰ intron in 25S rDNA of vaginal Candida albicans might be important in affecting itraconazole, fluconazole and nystatin susceptibility.

Objective:To study the content of total flavones in different parts of Mosla dianthera harvested in different seasons to pro-vide reference for the determination of optimal medicinal part and harvesting time of the plant. Methods:The total flavones content of M. dianthera was determined by TLC-UV. Results:The content of total flavones extracted from the plant in September was highest, and that in the leaves was much higher than that in the stems or the roots. Conclusion:It is advisable to harvest the aerial parts of M. dianthera in September.

Object To study the DNA fingerprinting of Arctium lappa L obtained from different localities Methods DNA fingerprinting of samples of crude and processed A lappa collected from four large commercial centers were examined by RAPD Results All crude A lappa showed similar fingerprinting characteristics, while the processed products gave considerable different results Conclusion DNA fingerprinting study is a reliant method to differentiate crude A lappa from its processed product

Objective To deeply understand the postoperative real experience of colostomy patients on ostomy management, and try to explore the difficulties and needs after discharge in order to provide theoretical basis for postoperative nursing intervention. Methods By using phenomenological research method, 10 colostomy patients were interviewed face- to- face, and the content was analyzed by Colaizzi method. Results Three themes were concluded: self- care obstacle related to ostomy; self- growth brought by ostomy; expectations for social support and family care. Conclusions Colostomy patients have to face so many problems after operation on ostomy management, and hospital, society and family have responsibility to help patients adapt to ostomy as soon as possible and improve their quality of life.

Objective To calculate the maximum ability of the mobile medical team ( MMT) to treat the wounded in order to provide reference for wartime medical support .Methods The flow of casualties between the main units in an MTT is described,data on training is collected through video analysis and questionnaires , the workflow of each unit above is mod-eled by the method of operational research using WINQSB software , and the theoretical maximum value is obtained .Results The theoretical maximum ability of a typical MMT to deal with the wounded is 322 per day when all the function units are operating at full load .Conclusion Under new circumstances , data on an MMT are different from those in the past .Reason-able organization is needed to increase the efficiency while the amount of evacuation should be maximized .

Objective: To observe the efficacy of electroacupuncture plus TDP for treating functional dyspepsia Methods: Sixty cases of functional dyspepsia were treated with electroacupuncture plus TDP, and another 60 cases were treated with Domperidone as a control. Results: After 10 treatments, the curative rate in treatment group was 33.3%, with a total effective rate of 91.7%. While in control group, the curative rate was 13.3%, with a total effective rate of 66.7%. There were significant differences between the two groups (P＜0.01,P＜0.001). Conclusion: Electroacupuncture plus TDP had the better therapeutic effect in treating functional dyspepsia.

The paper discusses the experimental teaching reform of biochemistry by referring to the characteristics of biochemical development and special feature.The reform increases effectually the interests of medical students,and may contribute to their creative competence and the abilities of scientific research.

Purpose:To investigate the effectiveness of intralymphatic infusion of anti- cancer agents and cytokines in the treatmrnt of malignancy.Materials anti methods:23 patients suffering from advanced metastatic cancers anti 2 primary lymphomas,unresponsble to the standard therapies or intra-arterial chemotherapy,were treated with lymphatic injections of an- ticancer ddrugs or combiation with biochemotherapy.Results:Follow-up study about one month after the therapy,comparing with findings on lymthatic radiographies anti computed tomographic scans,revealed decrease of lymphnodes in size in 23 cases.Conclusion:This therapeutic ap- proach proved to be an effective and safe method for the palliative treatment of advanced lym- phatic metastases and lymphomas.The procedure was feasible without serious compllications.

Object To explore the method for high quality DNA extract from plant Chinese medicinal materials containing developed xylem Methods The process of DNA includes wash with Tris buffer, extract with CYAB buffer and purification mainly Results Using this method, the total DNA was isolated from Cimicifugae foelida L., Arlia chinensis L., Paeoniae lactiflora Pall.,Aucklandia lappa Decne.,Lindera aggregata (Slms) Kostern., and Akebia quinata Decne. etc This method is preferable in removing pigments, polysaccharides, substance disturbing PCR Conclusion In this process, common reagents are used, lower cost is of advantage to popularize This study provids the applying foundations for the DNA identification of plant Chinese medicinal materials