The strategy of silico cloning based on the express sequence tags sequence (ESTs)and genome database is a new technique developed in recent years,and the core of the technique is to use bioinformatics technique to assemble and extend ESTs to get part of cDNA and even all sequence.It has the virtue of low investment,high speed,low and well-targeted technical requirements,etc.The relationship between human disease and genes has been widely confirmed,therefore,silico cloning technology will become the important means of disease research.This paper aims at elaborating the progress of silico cloning in medicine.

Acute liver failure (ALF) in children is severe syndrome with varied etiology and a rapid deterioration of liver function which results in coagulopathy abnormality and encephalopathy.ALF in children often carries a high morbidity and mortality.Laboratory tests is helpful for diagnosis and severity assessment of ALF in children and is useful for predicting survival or need for liver transplantation.The objective of this article is to introduce the values of laboratory tests in clinical diagnosis,prognosis and timing for transplantation of ALF in children.

Objective To determine and compare the complete sequence of COII gene from Anopheles sinensis, Aedes albopictus and Culex quinquefasciatus and to identify the molecular homology among these mosquitoes. Methods The recombinant plasmids including COII gene from the three species of mosquitoes were obtained by the T A cloning technique,and the COII genes were sequenced and analyzed. Results The homology of the COII gene nucleotide sequence and the deduced amino acid sequence of COII among the three species of mosquitoes varied from 84.1% to 87.9% and from 85.1% to 89.5%, respectively. The C+G content of COII gene was 23.2%-24.9%, the frequency of transversion higher than that of transition. Conclusion Aedes albopictus and Culex quinquefasciatus are closely related species at their COII molecular level according to COII gene.

Angiogenesis plays an important role in the process of the repair and regrowth of tissues. So far, many growth factors have been shown to have the angiogenic properties. Angiogenesis therapy using exogenous angiogens or the DNA coding for these growth factors has aroused extensive interest. The advances concerning angiogens and angiogenesis therapy applied in reconstructive surgery are reviewed in this paper.

Objective To evaluate the safeness of this treatment by comparing the serum IGF-Ⅰ and IGFBP3 concentration level before and after receiving rhGH treatment in pediatric patients.Methods 30 pediatric patients in our hospital receiving rhGH treatment were subjected to monitor serum IGF-Ⅰ and IGFBP3 level before and after the treatment and calculate the IGF-Ⅰ and IGFBP3 ratio.At the same time the serum T3,T4 and TSH are also assayed.Results The average increase of booly heights was(8.77?3.01)cm /y(t=7.773,P0.05),but the serum IGFBP3(t=3.759,P0.05).However,there was statistical significance of the serum IGFBP3 level between subgroups(f=22.964 P0.05).Conclusion The serum IGF-Ⅰ and IGFBP3 level are able to be used as one of clinical evaluation benchmarks to detect the risks of inducing tumor during the rhGH treatment in the pediatric patients.

Objective To investigate the effect of Danzhijiangtang Capsule on serum resistin level in insulin resistance (IR) rats induced by low dose of streptozotocin (STZ) and high calorie food. Methods Male Wistar rats were randomly divided into normal group, model group, low dose and high dose of Danzhijiangtang capsule treating group. The rats of normal group were fed with common food while the others with high calorie food and injected with low dose of STZ. The weight, fasting blood glucose (FBG), fasting insulin (FIns), blood fat, resistin level of all rats were measured, and insulin sensitivity index (ISI) was calculated accordingly. Results Danzhijiangtang capsule could reduce body weight of IR rats. According to the results of biochemical indicator detection, Danzhijiangtang capsule could reduce FBG and FIns level, improve blood fat condition, enhance ISI effectively and decrease resistin level (P

Aim To explore the effect of atorvastatin on NF-?B/I?B signal pathway,P selectin and ICAM-1 expression in cholesterol-rich diet-induced rabbit atherosclerosis.Methods 24 New Zealand white rabbits were randomly divided into three groups: control group,high-cholesterol group and atorvastatin group.Nucleus NF-?B p65 and cytoplasma I?B? were detected with Western blot technique.Plasma P selectin was detected with Immunoradioassay.Immunohistochemistry was employed to detect the expression of ICAM-1.Results Compared with the control group,the expression of nucleus NF-?B p65,P selectin and ICAM-1 increased significantly and cytoplasma I?B? reduced in high-cholesterol group(P

Objective To study the diagnosis and therapy of Hashimoto's disease (HD) with thyroid nodule. Methods The clinical data of 187 patients with nodulous Hashimoto's disease who had operation in our hospital from 1995 to 2008 were analysed retrospectively. Results The nodules coexisting with Hashimoto's disease were thyroid carcinoma, nodular goiter, thyroid follicular adenoma and lymphoma accounting for 11.2% (21/187), 26.7% (50/187), 15% (28/187) and 1.6% (3/187) respectively. Conclusions HD patients with thyroid nodules especially the single nodule that can not be distinguished from cancer should have operation in time. Ultrasound guided needle aspiration will be helpful for the preoperative diagnosis and the frozen section may guide the surgical modality.

Objective To establish a method for determination of lead in cosmetics. Methods Samples were digested. The lead content was detected by isochronous mercury-plating method with the condition of lead cation being enriched for 40 s at -1.20V(vs. SCE). Results The stripping potential peak of lead appeared at - 0. 20 V( vs. SCE) . The detection limit was 0.03 jug Pb. Six parallel determinations of a sample were done with the mean of 3.574mg/ kg and the relative standard deviation of 4. 1% . Recovery rates obtained by standard addition test ranged from 90% -100.5% . Conclusion The method is suitable for detecting lead in cosmetics.

PBL ( Problem-based learning) is a problem-based and student-centered learning pattern.PBL teaching method has been applied to evidence-based medicine ( EBM ) courses for international students.By this method,the students' learning enthusiasm was stimulated,the ability of ananlyzing and resolving problem was improved,and good teaching effect was achieved,which provided knowledge to improve further EBM teaching and evidence-based clinical practice.