1.Determination of procyanidin B2 in grape seed extract by RP-HPLC
Zhaogui CHEN ; Yanhua LU ; Dongzhi WEI
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To establish the method of determining procyanidin B2 content in grape seed extract by HPLC. METHODS: The determination was carried out with ZORBAX SB-C_ 18 column. The mobile phase consisted of acetonitrile-2% acetate buffer under the gradient elution condition, detection wavelength was at 280nm. RESULTS: There was a good linear relationship between the peak area and concentration in the range of 1~30 ?g/mL for procyanidin B2. The average of procyanidin B2 (n=5) was 99.29% and RSD=1.64%, respectively. CONCLUSION: The method is simple, accurate, reproducible and can be used for assay of procyanidin B2.
2.Effects of Faecalibacterium prausnitzii supernatant on Th17 cell and IL-17A in dextran sulfate sodium-induced ulcerative colitis mice.
Xiaoli HUANG ; Xin ZHANG ; Xianyan FEI ; Zhaogui CHEN ; Chengong YU
Journal of Central South University(Medical Sciences) 2015;40(12):1320-1326
OBJECTIVE:
To explore the protective and therapeutic effects of Faecalibacterium prausnitzii (Fp) supernatant on ulcerative colitis (UC) in mice induced by dextran sulfate sodium (DSS) and the underlying mechanisms.
METHODS:
Forty male mice were randomly divided into a control group, a model group, a treatment group and a prevention group (n=10 in each group). The colorectal histopathologic damage score (HDS) were calculated; the proportion of helper T cell (Th17) in mononuclear cells (MNC) in spleen, the levels of IL-17A and IL-6 in plasma were detected; the mRNA levels of transcription factor retinoic acid-related orphan receptor-γt (ROR-γt), interleukin (IL)-17A and IL-6 in colon mucosa tissues were also determined.
RESULTS:
Compared with the model group, the colon HDS in the treatment group and the prevention group were significantly decreased (both P<0.05), but there was no significant difference in the treatment group and the prevention group (P>0.05). The proportion of Th17 cells in spleen in the treatment group and the prevention group was also remarkably lower than that in the model group (both P<0.01). The levels of IL-17A and IL-6 in plasma in the treatment group were significantly lower than those in the model group (both P<0.05). The mRNA expression of ROR-γt, IL-17A and IL-6 in the colon mucosa tissues in the treatment group were remarkably lower than those in the model group (all P<0.05). But there was no statistic difference in the level of IL-6 in the plasma and the colon mucosa tissues between the prevention group and the model group (P>0.05).
CONCLUSION
Fp supernatant has protective and therapeutic effects on ulcerative colitis in mice induced by DSS, which might be mediated by decrease of Th17 and IL-17A levels in the plasma and the colon mucosa tissues. Fp supernatant also can decrease mice colitis by reducing IL-6 levels.
Animals
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Clostridiales
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Colitis, Ulcerative
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chemically induced
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immunology
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Culture Media, Conditioned
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pharmacology
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Dextran Sulfate
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adverse effects
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Interleukin-17
;
blood
;
immunology
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Interleukin-6
;
blood
;
immunology
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Intestinal Mucosa
;
immunology
;
Male
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Mice
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Nuclear Receptor Subfamily 1, Group F, Member 3
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metabolism
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Random Allocation
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Th17 Cells
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drug effects
;
immunology