Objective To evaluate the effect of Chinese herbal medicine immunomodulator on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obstructive jaundice. Methods Three-weeks Wistar rats were randomly divided into four groups. (n= 12, in each): normal control group, sham operation group, obstructive jaundice (OJ) group, OJ + Chinese herbal medicine immunomodulator (OJ+ZY) group. Except for the normal control group, the others' bile duct stones were ligatured to establish rat models with obstructive jaundice. The percentage of CD4+ and CD8+ tlymphocytes and the ratio of CD4+/CD8+ in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Results The percentage of CD4+ cell [(36.2 ±4.2)%, (28.8±1.8)% respectively] and the ratio of CD4+/CD8+ [(1.14±0.39), (1.37±0.34)respectively] in OJ group were lower than those in normal control group [peripheral blood: CD4+(41.5±5.3)%,CD4+/CD8+(1.37±0.19); intestinal mucosa: CD4+(32.3± 2.4)%, CD4+/CD8+ (1.84+0.28) and sham operation group (peripheral blood: CD4+ (41.2±5.5)%, CD4+/CD8+ (1.45±0.27); intestinal mucosa: CD4+(31.5 ± 2.7)%, CD4+/CD8+ (1.63±0.58)] . The difference was statistical significant(P<0.05). The percentage of CD4+ cell [(42.7±6.3)%, (33.6±2.4)% respectively] and the ratio of CD4+/CD8+ [(1.56±0.46), (1.84±0.56)respectively] in OJ+ZY group, were higher than those in OJ group(P<0.05). The difference was statistical significant(P<0.05). Conclusion Chinese herbal medicine immunomodulator can increase T lymphocyte immune function in immature rats with obstructive jaundice, but has no significance in normal control group as well as sham operation group.

Objective To evaluate the effect of Astragalus on T lymphocyte immune function in peripheral blood and intestinal mucosa of immature rats with obstructive jaundice. Methods Three-week-old Wistar rats were randomly divided into four groups(n=12,in each):control group, sham operation group, obstructive jaundice(OJ)group,OJ+Astragalus (OJ+A)group.The percentage of CD4 and CD8 T lymphocytes, the ratio of CD4 to CD8 in peripheral blood and intestinal mucosa of immature rats was detected by flow cytometry. Result The percentage of CD4+ cell and the ratio of CD4 to CD8 in the OJ group were lower than those in the control group and the sham operation group,with significant difference(P＜0.05).The percentage of CD4 cell and the ratio of CD4 to CD8 in the OJ+A group were higher than those in the OJ group(P＜0.05).Conclusion Astragalus can increase T lymphocyte immune function in OJ immature rats.

【Objective】 To investigate whether the current retention methods in blood stations can fully meet the traceability requirements of blood test results by analyzing the traceability of retained samples for syphilis antibody testing. 【Methods】 Thirty-four one-assay-positive deep-well plate retention samples, 68 double-assay-positive deep-well plate retention samples and 263 negative retention blood braids and corresponding deep-well plate retention samples that expired retention period for syphilis antibody testing from 2014 to 2020 in our center were collected. The TP-ELISA assays of two manufacturers were used for retesting, and the results were recorded and compared with the original results statistically. 【Results】 The concordance rate of the double-assay-positive and single-assay-positive samples with their corresponding deep-well plate samples was 98.53%(67/68) and 67.65%(23/34), respectively(P<0.05). Specific results for single-assay-positive syphilis antibody samples and their corresponding deep-well plate retention samples were as follows: 1) Single positive (reagent A): 13 out of 14 original samples were 0.65