1.Fluorescent antibiotics for real-time tracking of pathogenic bacteria
Lu MIAO ; Weiwei LIU ; Qinglong QIAO ; Xiaolian LI ; Zhaochao XU
Journal of Pharmaceutical Analysis 2020;10(5):444-451
The harm of pathogenic bacteria to humans has promoted extensive research on physiological processes of pathogens, such as the mechanism of bacterial infection, antibiotic mode of action, and bacterial antimicrobial resistance. Most of these processes can be better investigated by timely tracking of fluorophore-derived antibiotics in living cells. In this paper, we will review the recent development of fluorescent antibiotics featuring the conjugation with various fluorophores, and focus on their applica-tions in fluorescent imaging and real-time detection for various physiological processes of bacteria in vivo.
2.Rapid screening of SARS-CoV-2 inhibitors via ratiometric fluorescence of RBD-ACE2 complexes in living cells by competitive binding.
Lu MIAO ; Wei ZHOU ; Chunyu YAN ; Yuebin ZHANG ; Qinglong QIAO ; Xuelian ZHOU ; Yingzhu CHEN ; Guangying WANG ; Zhendong GUO ; Jun LIU ; Hailong PIAO ; Xia PAN ; Mengxue YAN ; Weijie ZHAO ; Guohui LI ; Yueqing LI ; Zhaochao XU
Acta Pharmaceutica Sinica B 2022;12(9):3739-3742