2.Advances in Medical Imaging Evaluation of Fatty Liver
Liming ZHAO ; Ningjing YANG ; Bin SONG
Chinese Journal of Bases and Clinics in General Surgery 2004;0(01):-
Objective To evaluate the value of medical imaging technology in qualitative and quantitative diagnosis of liver steatosis.Methods To describe the current status and advancements of medical imaging technology such as sonography,CT and MRI in qualitative and quantitative diagnosis of liver steatosis,and to contrast their advantages and shortages.Results Sonography could be used as the primary screening and evaluate measures in qualitative and quantitative diagnosis of liver steatosis,and CT was more reliable in quantitative diagnosis,MRI had significant improving with its high sensitivity and specificity.Conclusion Medical imaging technology has significant clinical value in qualitative and quantitative diagnosis of liver steatosis,especially with the help of functional MR imaging techniques such as spectroscopy and chemical shift Gradient-Echo technic.
3.Analysis on structured electronic medical record first page data in clinical application
Lin WU ; Yang ZHAO ; Yanli SONG
China Medical Equipment 2015;(12):44-46
Objective:Study on the clinical application and analysis of structured electronic medical records in our hospital in last.Methods: Discharged patients data selected from July 2012 to June 2014, using statistical methods for data processing, 8000 case files were divided into two groups: Group A and Group B.Results: After comparing the two groups of two groups of data, and ultimately disease coding accuracy rate 94.88%, fill in the correct rate increased 88.38%, missing rate decreased 4.63%, two groups had no significant difference data; The quality of writing the doctor increased gradually, the error rate tends to be stable, structured clinical application to control.Conclusion: Sequential specification writing medical doctor, scientific analysis of medical record information, the clinical application of structured electronic medical records the error rate is reduced in the operation, improve the accuracy, more standardized, accurate, standardized.
4.Arterial blood gas changes induced by CO_2 pneumoperitoneum in rabbits with lung and liver impact injury under controlled hemorrhage
Yong LI ; Lian-Yang ZHANG ; Song ZHAO ;
Chinese Journal of General Surgery 2000;0(12):-
Objective To investigate arterial blood gas(ABG)changes induced by different intra- abdominal pressures during CO_2 pneumoperitoneum in rabbits with lung and liver impact injury under controlled hemorrhage.Methods In this study 75 rabbits were randomized into nine groups(6 rabbits in each group)according to the amount of blood loss(6 ml/kg、12 ml/kg、22 ml/kg)and intra-abdominal pressures(5 mm Hg,10 mm Hg,15 mm Hg).After model was established successsfully,respiratory rates (RR),ABG and death rates were observed at pre-pneumoperitoneum,after 0.5 hour and 2 hours under pneumoperitoneum and 0.5 hour after desufflation.Results Without pneumoperitoneum,different blood loss exerted different effect on arterial blood gas:RR,PaCO_2 increased(P
5.A study on laser channels and myocardial angiogenesis after transmyocardial laser revasculariaztion
Renyao ZHANG ; Bo SONG ; Zhao YANG
Chinese Journal of Interventional Cardiology 1993;0(02):-
Objective To investigate the effects of traditional transmyocardial laser revascularization (T-TMLR) and non-transmural myocardial laser revascularization (N-TMLR) on myocardial ischemic and necrotic areas and angiogenesis. Methods In rabbit models, using Evans′ blue -TTC stain and HE stain technique for measuring ischemic and necrotic areas and observing angiogenesis. Results After T-TMLR and N-TMLR,compared with the control group, the ischemic areas significantly decreased and significant elevation of angiogenesis was observed. But no difference in necrotic areas was observed among the three groups. Conclusion N-TMLR can achieve the same effects with T-TMLR. Inducing angiogenesis via laser channels and decreasing ischemic area may be one of the predominant mechanisms of TMLR whereas laser channels themselves open or not has no relation to the effects of TMLR.
6.Expression and significance of histidine decarboxylase in liver and intestinal tissue in rats with strangulated intestinal obstruction
Yinzhi JIN ; Yang ZHAO ; Bin SONG
International Journal of Surgery 2015;42(7):442-445,封3
Objective To investigate the expression and significance of Histidine decarboxylase (HDC) in liver and intestinal tissue for early diagnosis of strangulated intestinal obstruction.Methods Ten male Wistar rats for the control group and 20 ones for the test group which was divided into A,B groups.Group A (n =10) was made by using ligating ileum for 1 h and group B (n =10) was for 3 h.When intestinal ob struction models were built,the changes of pathology in liver and intestine tissue were observed by light microscope.Real time quantitative PCR was used to detect the expression of HDC in liver and intestinal tissue.Results Under light microscopic level,the injury score of intestine in test group was increased in comparison with that in control group.At 3 h after strangulated intestinal obstruction,RT-PCR showed that brightness of HDC band in intestine tissue was significantly higher than the control.Real time quantitative PCR also showed that the expression of HDC in liver tissue in group A and group B increased 1.34 fold(P >0.05) and 2.21 fold (P < 0.05) respectively,when compared with the control group.The expression of HDC in intestine tissue in group A and group B increased 1.81 fold(P > 0.05) and 8.02 fold (P < 0.01) respectively,when compared with the control group.Conclusion Expression of HDC can be used for early diagnosis of strangulated intestinal obstruction.
7.Prevention and Treatment Policy of Pan-drug Resistant Bacteria for Surgical Critical Patients
Xiaowen WANG ; Xiukai CHEN ; Song ZHAO ; Yang SONG
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To investigate the distribution scope of the pan-drug resistant bacteria in the SICU patients including their producing environment and high risk factors in these two years and to approach its prevention and treatment policy. METHODS The distribution scope and the high risk factors of the pan-drug resistant bacteria in SICU were reviewed and analyzed from Jan 2005 to Jan 2007.RESULTS There were 11 cases in the period from Jan 2005 to Jan 2006; and was only 1 case from Jan 2006 to Jan 2007. CONCLUSIONS To establish the drug resistance monitoring system, attach great importance to isolation and education, to monitor the original region of the pan-drug resistant bacteria, and to adopt the comprehensive antibiotic policy to control the drug resistant bacteria. Among them, the early effective isolation of high risk patients may be very effective to reduce the producing and developing of the pan-drug resistant bacteria.
8.Histological observation on the urinary organs in normal Meriones Unguieulataus
Wenjie ZHAO ; Zhiqi SONG ; Guangyu CHENG ; Deming ZHAO ; Lifeng YANG
Chinese Journal of Comparative Medicine 2014;(12):24-26
Objective Mongolian gerbil can make themself urine concentration for saving water and adapt to the harsh desert environment, due to their very unique moisture control system in the body.Methods Mongolian gerbil is resistant to drought on account of their special kidney. Histology of the kidney, ureter and bladder in Meriones Unguiculataus were observed by light microscopy using HE staining.Results The results showed that compared with rats and mice, the Mongolian gerbils have more developed distal tubules, and well developed inner renal medulla.Conclusions We hope that the findings of this study enrich our understanding of the histology of urinary system in Mongolian gerbils and provide support for the laboratory animalization of this animal.
9.Determination of Chlorogenic Acid and Rutin in Tibetan Medicine Nettle by HPLC
Duanwei ZHAO ; Shusheng YANG ; Jianbang ZHAO ; Pingshun SONG
China Pharmacist 2016;19(7):1409-1410
Objective:To establish a method to determine the content of chlorogenic acid and rutin in nettle to assess the quality of nettle.Methods:An HPLC method was used with the following chromatographic conditions:CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm) was used with the mixture of 0.4%phosphoric acid and methanol as the mobile phase with gradient elution , the de-tection wavelength was set at 340 nm, the flow rate was 1.0 ml· min-1 and the sample size was 10μl.Results:Chlorogenic acid with-in the range of 2.360-23.600μg · ml-1 showed a good linear relationship (r=0.999 9), and rutin within the range of 6.208-62.080μg · ml-1 showed a good linear relationship (r=0.999 9).The recovery of rutin and chlorogenic acid was 99.20% and 100.40%with RSD of 1.2%and 1.1%(n=6), respectively.Conclusion:The method is fast, effective, simple, accurate and reproducible , which can be used to quantitatively analyze chlorogenic acid and rutin in nettle .