Objective To analyze the clinical efficacy and safety of laparoscopic and open uterine myomectomy.Methods 160 cases of uterine fibroids diagnosed by our hospital from April 2012 to April 2016 were selected as study cases.The patients were divided into two groups according to the order of treatment.80 patients in the control group received open uterine myoma rejection.80 patients in the observation group received laparoscopic myomectomy.The clinical treatment effect and complication were analyzed.Results The operation time,amount of intraoperative blood loss and the incision size of the observation group were (78.46 ± 10.31)min,(86.24 ±7.65) mL,(25.36 ± 1.28) mm,respectively,which were significantly less than those in the control group,the differences were statistically significant (t =6.57,6.81,7.66,all P ＜ 0.05).The time of postoperative body temperature recovery,time of anal exhaust,time of bedtime and the hospitalization time of the observation group were (24.16 ±3.81)h,(18.29 ± 4.36)h,(11.38± 2.06)h,(4.63 ± 1.51)d,respectively,which were significantly shorter than those in the control group (t =6.25,7.38,7.70,6.27,all P ＜ 0.05).The operative rate and postoperative analgesia rate of the observation group were 6.25％ and 11.25％,respectively,which were significantly lower than those of the control group (x2 =8.33,9.07,all P ＜ 0.05).The incidence rate of postoperative complications was 6.25％ in the observation group,which was significantly lower than 25.00％ in the control group,the difference was statistically significant (x2 =8.65,P ＜ 0.05).Conclusion Laparoscopic myomectomy has the advantages of less trauma,less bleeding,quick recovery and less complication,and high safety.It is the ideal technique for the treatment of uterine fibroids,which is worthy of promotion and application.

Objective To investigate the distribution and synaptic connectivity of neuropeptide Y (NPY)-like immunoreactivity (IR) in Bullfrog retina, Rana Calesbeiana, especially in the inner plexiform layer. Methods Light microscopy immunofluorescence and post-embedding colloidal gold labeling electron microscopy techniques were employed in this study. Results NPY immunoreactivity was associated with a small population of amacrine cell bodies in the inner nuclear layer and cell processes, ramified with 3 distinct bands, in the inner plexiform layer. As for ultrastructure, quantitative analysis showed that NPY-IR amacrine cell processes were most often presynaptic to NPY negative amacrine cell processes (49.7%) and ganglion cell dendrites (49.3%). NPY-IR amacrine cells predominantly received synaptic input from bipolar cell axon terminals (86%), while a few input from other amacrine cell processes (14%). Conclusion NPY-IR in Bullfrog retina is mainly distributed in the inner part and predominately receives excitatory glutamate modulation from bipolar cells.

OBJECTIVE To investigate the feature of nosocomial infections of our hospital inpatients,and analyze the correlative factors,based on which effective methods are taken.METHODS An analysis was made on the basis of the survey,the data came from 58 324 hospitalized cases from 2003 to 2006.RESULTS Of them 2 509 cases were infected,the incidence was 4.30%.The lower respiratory tract ranked the first place (26.66%)and the highest infected rate was in Hematology Department(7.79%).The species were predominated by Gram-negatives(58.04%).CONCLUSIONS Strengthening the nosocomial infection management,the infection rate could be decreased after taking prevention measures according to various correlative factors.

Objective: To study the optimum process of Ganmao Capsules(Rhizoma Coptidis, Herba Ephedrae, etc.). Methods : The extraction of Rhizoma Coptidis and Herba Ephedrae compared with Rhizoma Coptidis according to amount of berberine. The orthogonal design was used to select the optimum extraction according to amounts of berberine and ephedrine. Results : The extraction of Rhizoma Coptidis was better than that mixed Rhizoma Coptidis and Herba Ephedrae. Taking water as solvent, Rhizoma Coptidis was macerated for 0.5h and extracted in sequence three times with 12, 10 and 10 times volume of water extraction time for 1.5h, 1.0h and 1.0h, respectively. And Herba Ephedrae was macerated for 0.5h and extracted in sequence three times with 10, 8 and 8 times volume of water extraction time for 1.5h, 1.0h and 1.0h. Conclusion : The experimental results provided the basis for the ascertainment of extraction process of Rhizoma Coptidis and Herba Ephedrae preparation.

Abstract?AlM: To study the effect of latanoprost combined with timolol treatment on visual function, intraocular pressure and ocular blood flow of open angle glaucoma.?METHODS:A total of 50 cases ( 59 eyes ) with open angle glaucoma were enrolled from January 2012 to May 2014 in our hospital department of ophthalmology, and divided into observation group and control group by adopting the random number table method. Patients in observation group were treated with latanoprost combined with timolol, patients in control group were treated only with timolol. Visual function, intraocular pressure and ocular blood flow were compared.?RESULTS:1, 2, 3 and 4wk after treatment, observation group during the day intraocular pressure, night intraocular pressure were significantly lower than that in control group, vision levels (0. 27±0. 03, 0. 36±0. 06, 0. 44± 0. 06, 0. 63 ± 0. 13 ) were significantly higher than that in control group; observation group peak systolic velocity (14. 41± 1. 73) cm/s, end diastolic velocity (4. 18 ± 0. 67) cm/s were significantly higher than that in control group;vascular resistance index ( 0. 58 ± 0. 07 ) was significantly lower than that in control group.? CONCLUSlON: Latanoprost combined with timolol treatment can reduce intraocular pressure, increase blood flow of central retinal artery, reduce vascular resistance, improve visual acuity.

OBJECTIVE: A series of Bobath concept, Bobath theoretical hypothesis and clinical practice were summarized, to proVide a better understanding of Bobath concept involved in nervous tissues repairing and functional reconstruction. METHODS: Articles concerning the Bobath treatment for stroke-related literature were retrieved in Highwire press, MEDLINE, and Google from 2001 to 2009, using the key words of "Bobath concept, stroke physical therapy". Data of each study were extracted and analyzed following retrieval. Inclusion criteria:①Bobath concept and its theoretical hypothesis. ②New development of Bobath concept in clinical practice. Exclusion criteria: Repetitive studies. The results of retrieved literatura were analyzed. RESULTS: The theoretical basis of Bobath concept is based on present-day knowledge of motor control, motor learning, neural and muscle plasticity, and biomechanics. The latest theories and viewpoints are rasourced from International Bobath Instructors Training Association and the British Bobath Tutors Association. They expounded on the Bobath concept from linking participation, activities and underlying impairments, organization of human behavior and motor control, the consequences of injury and dysfunction in the execution of movement, neural and muscle plasticity, motor learning and so. This article also discusses several important questions which meet in clinical practice. CONCLUSION: Bobath therapy, as a traditional method for stroke rehabilitation, is constantly absorbing the research results from current neuroscience, psychology and other disciplines, and blended with other treatments to promote the development of neuro-rehabilitation medicine.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; Humans ; Phytotherapy

BACKGROUND:Cartilage scaffold plays an important role in articular cartilage injury, but it is stil restricted in practice, leading to the repair failure using tissue-engineered cartilage. OBJECTIVE:To explore the preparation method of cartilage scaffold with directional structure and its repairing effect in cartilage injury of rabbits. METHODS:Directional cartilage scaffolds were prepared by temperature gradient induced phase separation technique. The cross-linking of cartilage scaffold was completed with genipin. The physical and mechanical properties of scaffolds were determined. Forty-eight white rabbits were modeled into exercised-induced articular cartilage injury and equivalently randomized into two groups, fol owed by implantation of non-directional or directional scaffolds. Samples were col ected at postoperative 6, 12 and 24 weeks to compare the repair effect of two scaffolds on rabbit joint injury. RESULTS AND CONCLUSION:Scanning electron microscope showed that the regular microstructures on the longitudinal section of directional scaffolds were aligned in the same direction, while the longitudinal and transverse planes of the non-directional scaffolds showed porous honeycomb structures. The elastic modulus of the directional scaffold was significantly higher than that of the non-directional scaffold (P<0.05). O’Driscol histological scores in the directional scaffold group were significantly higher than those in the non-directional scaffold group at 6, 12 and 24 weeks postoperatively (P<0.05). At postoperative 24 weeks, in the directional scaffold group, the three-dimensional reconstruction of the rabbit defect region was flat and smooth, and coronal two-dimensional scanning showed the cartilage thickness of the newborn cartilage was very close to that of the normal bone;the cartilage surface in the non-directional rabbits was flat under three-dimensional CT, and coronal two-dimensional scanning showed that the repaired cartilage was almost the same as the normal cartilage. Hematoxylin-eosin staining showed that at 24 weeks postoperatively, the defect site disappeared and the cartilage cel s arranged in the vertical direction in the directional scaffold group. There were typical cartilage lacunae at the defect site in the non-directional scaffold group at 24 weeks postoperatively. Our findings suggest that the directional cartilage scaffold prepared using temperature gradient induced phase separation technique holds good physical and mechanical properties, and its implantation into the rabbit articular cartilage injury induced by exercise can promote the injury repair to obtain ideal mechanical properties.

Objective] The mechanism of astragalus polysaccharides treatment of asthma was discussed. [Methods]Through the domestic and foreign about traditional Chinese medicine monomer astragalus polysaccharide on IL-4, IL-10 and other cytokines and inflammatory infiltrates aspects such as the Th1/Th2 balance, smooth muscle actin and airway mucus in the airway remodeling and asthma related gene research literature summary. [Results] Improve airway inflammation astragalus polysaccharides from activation and inhibition EOS EOS infiltration reduction, regulating Th1/Th2 imbalance, so Th0 cells to Th1 cell differentiation, and inhibiting the release of inflammatory cytokines and other aspects, APS can reduce airway hyper-responsiveness mitigate aspects peribronchial infiltration of eosinophils and airway smooth muscle cells induced byα-SMA expression and other high alleviate airway remodeling. [Conclusion] The influence of astragalus polysaccharide on asthma gene research will lay the basis for study of the mechanism of treatment of asthma, monomer research provides new ideas for traditional Chinese medicine.

Objective] The treatment of pediatric asthma in remission period is summarized from the positive and negative aspects, in order to provide new ideas and methods for clinical treatment. [Methods] By consulting the related literatures on the traditional Chinese medicine treatment of children with asthma in remission phase, the paper mainly summarized the different physicians' understanding of pathogenesis and approaches to the treatment of pediatric asthma in remission stage. [Results]The treatment of physicians for pediatric asthma in remission phase can be divided into asthenia healthy qi and sthenia pathogenic factor syndrome.The treatment of healthy qi deficiency mainly includes treating lung,spleen,kidney seperately;curing lung-spleen,lung- kidney, spleen-kidney ;treating three viscera meanwhile.The therapy of pathogenic excess contains treating wind,phlegm,stasis respectively;curing phlegm-stasis. [Conclusion]The treatment of traditional Chinese medicine for pediatric asthma has a unique advantage in improving children's physique, reducing the frequency of asthma attacks, and improve children's life quality which has significant clinical effect.