Objective To investigate the distribution and synaptic connectivity of neuropeptide Y (NPY)-like immunoreactivity (IR) in Bullfrog retina, Rana Calesbeiana, especially in the inner plexiform layer. Methods Light microscopy immunofluorescence and post-embedding colloidal gold labeling electron microscopy techniques were employed in this study. Results NPY immunoreactivity was associated with a small population of amacrine cell bodies in the inner nuclear layer and cell processes, ramified with 3 distinct bands, in the inner plexiform layer. As for ultrastructure, quantitative analysis showed that NPY-IR amacrine cell processes were most often presynaptic to NPY negative amacrine cell processes (49.7%) and ganglion cell dendrites (49.3%). NPY-IR amacrine cells predominantly received synaptic input from bipolar cell axon terminals (86%), while a few input from other amacrine cell processes (14%). Conclusion NPY-IR in Bullfrog retina is mainly distributed in the inner part and predominately receives excitatory glutamate modulation from bipolar cells.

OBJECTIVE To investigate the feature of nosocomial infections of our hospital inpatients,and analyze the correlative factors,based on which effective methods are taken.METHODS An analysis was made on the basis of the survey,the data came from 58 324 hospitalized cases from 2003 to 2006.RESULTS Of them 2 509 cases were infected,the incidence was 4.30%.The lower respiratory tract ranked the first place (26.66%)and the highest infected rate was in Hematology Department(7.79%).The species were predominated by Gram-negatives(58.04%).CONCLUSIONS Strengthening the nosocomial infection management,the infection rate could be decreased after taking prevention measures according to various correlative factors.

Objective To analyze the clinical efficacy and safety of laparoscopic and open uterine myomectomy.Methods 160 cases of uterine fibroids diagnosed by our hospital from April 2012 to April 2016 were selected as study cases.The patients were divided into two groups according to the order of treatment.80 patients in the control group received open uterine myoma rejection.80 patients in the observation group received laparoscopic myomectomy.The clinical treatment effect and complication were analyzed.Results The operation time,amount of intraoperative blood loss and the incision size of the observation group were (78.46 ± 10.31)min,(86.24 ±7.65) mL,(25.36 ± 1.28) mm,respectively,which were significantly less than those in the control group,the differences were statistically significant (t =6.57,6.81,7.66,all P ＜ 0.05).The time of postoperative body temperature recovery,time of anal exhaust,time of bedtime and the hospitalization time of the observation group were (24.16 ±3.81)h,(18.29 ± 4.36)h,(11.38± 2.06)h,(4.63 ± 1.51)d,respectively,which were significantly shorter than those in the control group (t =6.25,7.38,7.70,6.27,all P ＜ 0.05).The operative rate and postoperative analgesia rate of the observation group were 6.25％ and 11.25％,respectively,which were significantly lower than those of the control group (x2 =8.33,9.07,all P ＜ 0.05).The incidence rate of postoperative complications was 6.25％ in the observation group,which was significantly lower than 25.00％ in the control group,the difference was statistically significant (x2 =8.65,P ＜ 0.05).Conclusion Laparoscopic myomectomy has the advantages of less trauma,less bleeding,quick recovery and less complication,and high safety.It is the ideal technique for the treatment of uterine fibroids,which is worthy of promotion and application.

Burns ; complications ; Humans ; Multiple Organ Failure ; etiology ; prevention & control

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; Humans ; Phytotherapy

Objective] The treatment of pediatric asthma in remission period is summarized from the positive and negative aspects, in order to provide new ideas and methods for clinical treatment. [Methods] By consulting the related literatures on the traditional Chinese medicine treatment of children with asthma in remission phase, the paper mainly summarized the different physicians' understanding of pathogenesis and approaches to the treatment of pediatric asthma in remission stage. [Results]The treatment of physicians for pediatric asthma in remission phase can be divided into asthenia healthy qi and sthenia pathogenic factor syndrome.The treatment of healthy qi deficiency mainly includes treating lung,spleen,kidney seperately;curing lung-spleen,lung- kidney, spleen-kidney ;treating three viscera meanwhile.The therapy of pathogenic excess contains treating wind,phlegm,stasis respectively;curing phlegm-stasis. [Conclusion]The treatment of traditional Chinese medicine for pediatric asthma has a unique advantage in improving children's physique, reducing the frequency of asthma attacks, and improve children's life quality which has significant clinical effect.

Objective To investigate the pathogenesis of gastroparesis in diabetic rats.Methods SD rats were randomly divided into control group and diabetes group.Ten weeks later,gastroparesis model was established.The apoptosis rate and mitochondrial membrane potential of the gastric smooth muscle cells were detected by flow cytometry.Cytochrome C was detected by immunohistochemistry.Results Compared with rats in control group:(1)Typical symptoms of diabetes mellitus appeared;The rate of gastric residual pigment was significantly higher(P

Objective To present a solution of the hospital information management access control for more secure information sharing. Methods Several commonly used access control models were compared. Some problems of the RBAC model were analyzed in the hospital information circumstances. Results A solution of space-based data access control was presented on the base of the role-based access control (RBAC) module. Conclusion The space-based data access control method can reduce space consumption and maintain data consistency, which is more suitable for the use of the hospital management information system.

Version 2015 of the National Comprehensive Cancer Network (NCCN) Guidelines for Uterine Neoplasms uses the 2009 International Federation of Gynecology and Obstetrics (FIGO) staging system.These NCCN guidelines divide pure endnmetrioid cancers into three categories to delineate treatment:(1) disease limited to the uterus;(2) cervical involvement;(3) extrauterinc disease.Surgery is recommended for medically operable patients.Continuous progestin-based therapy may be considered for highly selected patients with stage IA disease who wish to preserve their fertility.Preoperative chemotherapy can be considered if extrauterine disease is suspected.Factors that may influence the decision regarding adjuvant therapy include lymph vascular space invasion (LVSI),patient age,tumor volume,and lower uterine segment or surface cervical glandular involvement.When administering adjuvant radiotherapy,it should be initiated as soon as the vaginal cuff has healed,no later than 12 weeks after surgery.

Objective]The paper summarizes academic thought of Professor ZHAO Zhiqiang syndrome differentiation and treatment of adenomatous polyp of colon. [Method]By learning from ZHAO Zhiqiang and studying the medical record of adenomatous polyp of colon,sum up professor ZHAO Zhiqiang′ s experiences from etiology and pathogenesis, differential treatment, administered medical herbs and prescription ,and with one case for detailed explanation. [Results] Professor ZHAO thinks that the pathogeny raletes to exopathy, diet, emotion, weakness of the spleen and stomach, physique, smoke poison,influence of other diseases,the key of pathogenesis is phlegm, blood stasis and toxin stagnation, accumulation of intestine, Qi is out of adjustment, the conduction loss of us, He believes that the syndrome diagnosis of the disease is different from others, the syndrome diagnosis should include disease syndrome elements, syndrome elements of disease location and morbid state, disease syndrome elements should need the help of the modern medical endoscope and pathology, and ill treatment to detoxify the anti-cancer variable as the core, supplemented with Ruanjian Sanjie,coordinating zang and fu, modification according to symptoms.[Conclusion] Professor ZHAO's treatment of adenomatous polyp of colon has rich experience and prominent features, it is worth clinical promotion.