The Information and Management Platform of Standardized Training of Residents in Liaoning Province is a comprehensive management platform with information network , containing the whole process of the standardized training for residents. Liaoning, the populous province, has too many management staff in training residents, so it is very necessary to explore the information-based method for the training management. This platform has realized the whole-course network management of resi-dent training, and many common functions such as the application of training base , on-line registra-tion, on-line real-time monitoring of trainees' daily training state, on-line graduation exam arrangement, and inquiry of statistical information of trainees in the province . It saves human and management costs , improves management efficiency , and orderly pushes forward the standardized of residents training.

[Objective] To investigate the effect of Suiqing Pill (SP), a Chinese prescription with the actions of activating blood and removing blood-stasis, on growth and metastasis of tumor. [Methods] Sixty C57BL/6 mice were randomized to three groups: group A (model control), group B (low-dose SP) and group C (high-dose SP). H22 hepatocarcinomatous metastasis models were established by subcutaneous injection of H22 hepatocarcinoma cells into the right lower limb. The inhibitory rate of tumor weight, incidence of metastatic carcinoma of lung, metastasis of lymph node, survival time and life-span prolongation rate were observed. [Results] The weight of tumor was reduced and the inhibitory rate of tumor weight was 36.83% in group B (P

Objective To analyze the CT features of hepatic artery and morphology changes of the liver in patients with cavernous transformation of the portal vein (CTPV). Methods Thirteen patients of CTPV without malignant hepatic tumor or other diseases being able to cause changes of hepatic artery such as cirrhosis and hepatophyma were collected. CT data was analyzed, and the arterial and morphology changes of the liver were observed. Results Left and (or) right hepatic arteries were found dilated in 10 patients (76.92%), and unusual flake like enhancement was found in artery phase in 3 patients (23.08%). Atrophy-hypertrophy complex (AHC) could be found only in 1 patient (7.69%). Conclusion Dilatation of hepatic artery manifests more hepatic artery blood supply, while obvious AHC is hardly found in CTPV.

Object To develop a method for the quantitative analysis of peiminine, imperialine and imperialine 3? D glucoside in Fritillaria L by capillary electrophoresis Methods 9 species of bulbus Fritillaria L. were analysed with internal standard by the mode of capillary zone electrophoresis Results The 3 alkaloids were separated completely with the method which showed good recovery and reproducibility Conclusion The method was proved to be quick, simple, and efficient, and provided a reliable basis for the quality control and evaluation of this plant medicine

Objective To investigate the changes of coagulation and fibrinolysis systems in patients with solid cancer. Determine the mechanism of thrombosis formation in solid cancer and the the metastasis mechanism of solid cancer.Methods To measure plasma concentrations of TF,TFPI,t-PA,u-PA,PAI-1 by ELISA and test protein C activity(PC:A)by chromogenic substrate assay.Results Plasma concentrations of TF,TFPI,u-PA,PAI-1 were all higher in solid cancer patients than normal control and higher in metasta- sized team than non-metastasized team.In the dead team,u-PA and PAI-1 were higher while TFPI was low- er. t-PA was higher in cancer patients combined with venous thromboembolism while protein C activity was lower.Conclusion Disorders of coagulation and fibrinolysis system are related to the thrombosis formation in solid cancer.Coagulation and fibrinolysis factors take part in the metastasis progress of solid cancer. High concentration of u-PA and PAI-1 or low concentration of TFPI are considered to be related with poor outcome.

In this study we implanted magnetically labeled neural stem cells (NSCs) in PD rats and then monitored their survival and migration in the host brain by magnetic resonance imaging (MRI). The mesencephalic NSCs were obtained from the brain of SD rats. Superparamagnetic iron oxide (SPIO) was transferred to NSCs by Lipofectamine transfection. Eighteen PD lesioned rats were selected for transplantation by evaluation of their rotational behavior in response to amphetamine and randomly assigned to 3 groups, i.e., sham group, PBS group and NSCs transplanted group, with 6 rats in each group. MR scanning was performed at 1, 2, 4, 6, 8 and 10 week(s) following transplantation. At the meantime, rotational behavior was assessed in each group. Our results showed that SPIO particles were clearly visible with Prissian blue staining in neurospheres and cells derived from NSCs. The rotational behavior of the NSCs transplanted group was remarkably improved compared with that of sham group and PBS group (P < 0.05). In vivo MR tracking of NSCs showed that SPIO labeling led to a strong susceptibility change of signal 1 week after transplantation on T2 weighted images. And a large circular hypointense signal appeared in the transplanted area on T2* gradient echo images. Ten weeks following transplantation, the hypointense signal on T2 weighted and T2* gradient echo images was still displayed. It is concluded that SPIO particles could label NSCs effectively, and MRI detection of SPIO labeled cells is a promising method and novel approach to analyzing the NSCs following transplantation in the treatment of PD.

Objective To investigate the expression and clinical significance of interleukin -6 (IL -6)in aggressive and indolent lymphoma patients.Methods Serum specimens obtained from 1 07 non -Hodgkin's lympho-ma (NHL)patients and 50 healthy controls were collected.Chemiluminescence immunoassay was used to assess the expression of IL -6 in the serum.Results The level of serum IL -6(1 8.54 ±4.53)ng/L in aggressive lymphoma group was significantly higher than (6.90 ±1 .78)ng/L in the inert lymphoma group,and the serum IL -6 levels of two groups were significantly higher than the healthy control group (3.87 ±0.76)ng/L.The lymphoma patients,with B symptoms,elevated CRP or bone marrow involvement presented higher IL -6 level than the other patients (P <0.05).IL -6 level of the IPI high -risk group was higher than the intermediate -risk group and low -risk group, with significant difference (P <0.05 ).The correlation study showed that IL -6 and CRP,IPI /FLIPI,Ann Arbor stage,B symptoms,bone marrow involvement had significantly positive correlation (P <0.05);Through analysis of the effect of the patients with aggressive lymphoma,the serum level of IL -6 in patients with complete remission was sig-nificantly higher than the non complete remission group.By Logistic regression analysis,IL -6 was one of the inde-pendent risk factors affecting the treatment effect of NHL,and the difference was statistically significant (P <0.05). Conclusion The serum IL -6 was significantly elevated in NHL patients,in association with tumor invasion,progno-sis and therapeutic effect.IL -6 may help the diagnosis and prognosis of NHL.

Objective To investigate the analgesic efficacy and spinal neurotoxicity of intrathecal (IT) different doses of dexmedetomidine in rats. Methods Sixty male SD rats weighing 180-220 g were randomly divided into 5 groups ( n = 12 each): groupnormal control (group C); group IT normal saline (group N); different doses of dexmedetomidine groups received IT dexmedetomidine 0.75, 1.50 and 3.00 μg/kg respectively (groups D1.3). Paw withdrawal threshold to mechanical stimulation (PWMT)with yon Frey filaments and tail flick latency (TFL) to a thermal nociceptive stimulus were measured before (To, baseline) and at 30 or60 rin after IT dexmedetomidine or normal saline administration (T1, T2 ) and the percentage of the maximum possible effect ( MPE ) was calculated. Lumbar segment of the spinal cord ( L4-6 ) was removed for microscopic examination and determination of c-Fos expression (by immuno-histochemistry) at 7, 24 and 48 h after IT dexmedetomidine or normal saline administration. Results PWMT, TFL and the percentage of MPE were significantly increased after IT dexmedetomidine as compared with the baseline values at T0 in groups D1-3 ( P ＜ 0.05). PWMT was significantly higher at T1 and TFL and the percentage of MPE were higher at T2 in groups D1-3 than in groups C and N,and in group D3 than in groups D1,2 ( P ＜ 0.05). At 7,24 h after IT dexmedetomidine c-Fos protein expression was significantly higher in group D3 than in groups C and N( P ＜ 0.05). There was no significant difference in c-Fos expression at 48 h after IT dexmedetomidine between group D3 and groups C and N ( P ＞ 0.05 ). At 24 h after IT dexmedetomidine c-Fos protein expression was significantly higher in group D3 than in other 4 groups( P ＜ 0.05). Slight spinal cord injury was observed at 24 h after IT dexmedetomidine in group D3. Conclusion IT dexmedetomidine has antinociceptive effect. High dose dexmedetomidine IT can produce transient reversible toxicity to the spinal cord.

Purpose To investigate the expression of Apaf-1 and Caspase-9 in prostate cancer ( PCa) and benign prostatic hyperplasia ( BPH) , and to analyze their correlation with clinicopathological parameters of PCa. Methods Immunohistochemistry was used to de-tect Apaf-1 and Caspase-9 protein in 45 cases of PCa and 60 cases of BPH. Results The positive rate of Apaf-1 and Caspase-9 in PCa tissues was significantly lower than that in BPH (P<0. 05). The expression of Apaf-1 and Caspase-9 was not correlated with the age of patients and distant metastasis (P>0. 05), but it was correlated with the pathological grade and clinical stage of PCa (P<0. 05). Conclusion Apaf-1 and Caspase-9 are lowly expressed in PCa. There is positive correlation between the expression of Apaf-1 and Caspase-9 (rs =0. 645, P<0. 01). Apaf-1 and Caspase-9 might be correlated with the carcinogenesis and development of PCa.

Objective To evaluate the effects of ischemic postconditioning on brain injury induced by myocardial ischemia-reperfusion (I/R) in diabetic rats.Methods Diabetes mellitus was induced by intraperitoneal streptozotocin 60 mg/kg and confirmed by blood glucose level ＞ 16.7 mmol/L.Thirty male Sprague-Dawley rats,weighing 220-280 g,in which diabetes mellitus was successfully induced,were randomly allocated into 3 groups (n =10 each) using a random number table:group sham operation (group S),group I/R and group ischemic postconditioning (group P).Myocardial I/R was induced by occlusion of the anterior descending branch of the left coronary artery in I/R and P groups.Group P received 3 cycles of 10 s reperfusion followed by 10 s ischemia at the end of myocardial ischemia.The rats were sacrificed at 120 min of reperfusion and the brains were removed for microscopic examination and for determination of cell apoptosis (by TUNEL) and expression of interleukin-6 (IL-6),IL-8,IL-10,glycogen synthase kinase-3 beta (GSK-3β) and phosphorylated GSK-3β (pGSK-3β) (by immuno-histochemistry).Apoptotic index was calculated.Results Compared with group S,apoptotic index was significantly increased,IL-6 and IL-8 expression was up-regulated,and IL-10 and pGSK-3β expression was downregulated in I/R and P groups (P ＜ 0.01).Compared with group I/R,apoptotic index was significantly decreased,IL-6 and IL-8 expression was down-regulated,and IL-10 and pGSK-3β expression was up-regulated in group P (P＜0.01).There was no significant difference in GSK-3β expression among the 3 groups (P ＞ 0.05).The pathologic changes were significantly attenuated in group P as compared with group I/R.Conclusion Ischemic postconditioning can attenuate brain injury induced by myocardial I/R in diabetic rats,and inhition of activity of GSK-3β may be involved in the mechanism.