OBJECTIVE To investigate the feature of nosocomial infections of our hospital inpatients,and analyze the correlative factors,based on which effective methods are taken.METHODS An analysis was made on the basis of the survey,the data came from 58 324 hospitalized cases from 2003 to 2006.RESULTS Of them 2 509 cases were infected,the incidence was 4.30%.The lower respiratory tract ranked the first place (26.66%)and the highest infected rate was in Hematology Department(7.79%).The species were predominated by Gram-negatives(58.04%).CONCLUSIONS Strengthening the nosocomial infection management,the infection rate could be decreased after taking prevention measures according to various correlative factors.

Objective To investigate the distribution and synaptic connectivity of neuropeptide Y (NPY)-like immunoreactivity (IR) in Bullfrog retina, Rana Calesbeiana, especially in the inner plexiform layer. Methods Light microscopy immunofluorescence and post-embedding colloidal gold labeling electron microscopy techniques were employed in this study. Results NPY immunoreactivity was associated with a small population of amacrine cell bodies in the inner nuclear layer and cell processes, ramified with 3 distinct bands, in the inner plexiform layer. As for ultrastructure, quantitative analysis showed that NPY-IR amacrine cell processes were most often presynaptic to NPY negative amacrine cell processes (49.7%) and ganglion cell dendrites (49.3%). NPY-IR amacrine cells predominantly received synaptic input from bipolar cell axon terminals (86%), while a few input from other amacrine cell processes (14%). Conclusion NPY-IR in Bullfrog retina is mainly distributed in the inner part and predominately receives excitatory glutamate modulation from bipolar cells.

Objective To analyze the clinical efficacy and safety of laparoscopic and open uterine myomectomy.Methods 160 cases of uterine fibroids diagnosed by our hospital from April 2012 to April 2016 were selected as study cases.The patients were divided into two groups according to the order of treatment.80 patients in the control group received open uterine myoma rejection.80 patients in the observation group received laparoscopic myomectomy.The clinical treatment effect and complication were analyzed.Results The operation time,amount of intraoperative blood loss and the incision size of the observation group were (78.46 ± 10.31)min,(86.24 ±7.65) mL,(25.36 ± 1.28) mm,respectively,which were significantly less than those in the control group,the differences were statistically significant (t =6.57,6.81,7.66,all P ＜ 0.05).The time of postoperative body temperature recovery,time of anal exhaust,time of bedtime and the hospitalization time of the observation group were (24.16 ±3.81)h,(18.29 ± 4.36)h,(11.38± 2.06)h,(4.63 ± 1.51)d,respectively,which were significantly shorter than those in the control group (t =6.25,7.38,7.70,6.27,all P ＜ 0.05).The operative rate and postoperative analgesia rate of the observation group were 6.25％ and 11.25％,respectively,which were significantly lower than those of the control group (x2 =8.33,9.07,all P ＜ 0.05).The incidence rate of postoperative complications was 6.25％ in the observation group,which was significantly lower than 25.00％ in the control group,the difference was statistically significant (x2 =8.65,P ＜ 0.05).Conclusion Laparoscopic myomectomy has the advantages of less trauma,less bleeding,quick recovery and less complication,and high safety.It is the ideal technique for the treatment of uterine fibroids,which is worthy of promotion and application.

Objective To study clinical effect of laparoscopy combined with gestrinone in treatment of ovarian endometriosis cyst and the prognoisi of patients. Methods A randomized, controlled, prospective study was used in this study, 78 patients with ovarian endometriosis cyst were selected in Sichuan province panzhihua city miyi people's hospital from august 2012 to august 2014, and were randomly divided into observation group and control group, 39 patients in each group. Patients in the observation group were treated with laparoscopy combined with gestrinone (2.5 mg/time, two times per week, for 6 months) intervention; patients in the control group were treated with laparoscopic intervention. After six months, the postoperative serum FSH (Follicle Stmi Ulating Hormone, FSH), Estradiol (E2) and Estradiol levels, ovarian biggest plane average diameter (D), the level of MMP-9 and TIMP -1 level, pregnancy outcome of two groups were compared and the outcome and the recurrence rate 18 months after treatment were followed up. Results Six months after treatment, the E2 and FSH levels in the observation group were better than control group, with statistically significant difference (P<0.01);the postoperative serum levels of MMP-9 and TIMP-1 in Observation group were better than control group, with statistically significant difference (P<0.01);there were 20 patients of D≤2 cm, 19 patients of D>2 cm, the postoperative ovarian shrink rate was 51.28% in the observation group; there were 5 patients of D≤2 cm, 34 patients of D>2 cm ovarian shrink ratio was 12.82% in the control group, the ovarian shrink ratio of observation group was obviously higher than control group, with statistically significant difference (P<0.01) . Follow-up for 18 months, the recurrence rate was 5.13%in the observation group, which was significantly lower than thatin the control group (25.64%), with statistically significant difference (P<0.05);the pregnancy rate was 66.66%and 30.77%in the Observation group and control group, respectively, the difference had statistical significance (P<0.01) . Conclusion Laparoscopic surgery combined with gestrinone can protect ovarian function of patients with ovarian endometriosis cyst, improve pregnancy outcomes, and reduce recurrence rate, and has good clinical value.

OBJECTIVE To provide evidences for working out methods of nosocomial infection surveillance on the basis of investigation of nosocomial infection in our hospital.METHODS By efforts of the professional staff of hospital,with cooperation of the clinic personnel infection management group,all patients were investigated by bed-visiting and patients medical records checking.The data were analyzed.RESULTS Of the 1261 patients investigated,the prevalence rate of NI was 5.15%.The common sites of nosocomial infections were lower respiratory tract(32.31%),upper respiratory tract(16.92%),surgical wound(9.23%)and peritoneal cavity(6.15%).CONCLUSIONS The prevalence survey is a rapid and efficient method,which could offer evidence of target surveillance of NI.

Abstract?AlM: To study the effect of latanoprost combined with timolol treatment on visual function, intraocular pressure and ocular blood flow of open angle glaucoma.?METHODS:A total of 50 cases ( 59 eyes ) with open angle glaucoma were enrolled from January 2012 to May 2014 in our hospital department of ophthalmology, and divided into observation group and control group by adopting the random number table method. Patients in observation group were treated with latanoprost combined with timolol, patients in control group were treated only with timolol. Visual function, intraocular pressure and ocular blood flow were compared.?RESULTS:1, 2, 3 and 4wk after treatment, observation group during the day intraocular pressure, night intraocular pressure were significantly lower than that in control group, vision levels (0. 27±0. 03, 0. 36±0. 06, 0. 44± 0. 06, 0. 63 ± 0. 13 ) were significantly higher than that in control group; observation group peak systolic velocity (14. 41± 1. 73) cm/s, end diastolic velocity (4. 18 ± 0. 67) cm/s were significantly higher than that in control group;vascular resistance index ( 0. 58 ± 0. 07 ) was significantly lower than that in control group.? CONCLUSlON: Latanoprost combined with timolol treatment can reduce intraocular pressure, increase blood flow of central retinal artery, reduce vascular resistance, improve visual acuity.

Objective: To study the optimum process of Ganmao Capsules(Rhizoma Coptidis, Herba Ephedrae, etc.). Methods : The extraction of Rhizoma Coptidis and Herba Ephedrae compared with Rhizoma Coptidis according to amount of berberine. The orthogonal design was used to select the optimum extraction according to amounts of berberine and ephedrine. Results : The extraction of Rhizoma Coptidis was better than that mixed Rhizoma Coptidis and Herba Ephedrae. Taking water as solvent, Rhizoma Coptidis was macerated for 0.5h and extracted in sequence three times with 12, 10 and 10 times volume of water extraction time for 1.5h, 1.0h and 1.0h, respectively. And Herba Ephedrae was macerated for 0.5h and extracted in sequence three times with 10, 8 and 8 times volume of water extraction time for 1.5h, 1.0h and 1.0h. Conclusion : The experimental results provided the basis for the ascertainment of extraction process of Rhizoma Coptidis and Herba Ephedrae preparation.

0.5,the variance 0.5,variance coefficient

Complications that follow ischemic stroke are common,frequency ranging from 28% to 95%.It may either directly lead to death or prevent optimal recovery.54% of deaths after ischemic stroke are attributed to medical complications.Most complications occur during the first week,and stroke severity is the most important risk factor.The common serious medical complications are chest or urinary tract infection,deep venous thrombosis,pulmonary embolism,cardiac events,gastrointestinal bleeding,hyperglycemia,pressure sores.Falls,shoulder pain and depression are also common complications.Most of medical complications are preventable or treatable to some extent if recognized,so preventive strategies and appropriate treatment should be employed as early as possible.Prevention,early recognition,and management complications could improve short-term and long-term prognoses after ischemic stroke.

Objective To investigate the pathogenesis of gastroparesis in diabetic rats.Methods SD rats were randomly divided into control group and diabetes group.Ten weeks later,gastroparesis model was established.The apoptosis rate and mitochondrial membrane potential of the gastric smooth muscle cells were detected by flow cytometry.Cytochrome C was detected by immunohistochemistry.Results Compared with rats in control group:(1)Typical symptoms of diabetes mellitus appeared;The rate of gastric residual pigment was significantly higher(P