ObjectiveTo investigate the effects of controlled subcutaneous analgesia(PCSA) with sufentanil in patients underwent abdominal surgery and the occurrence of adverse reactions. Methods90 patients underwent abdominal surgery were randomly divided intothree groups with 30 cases each. Group Ⅰ :sufentanil 0. 04μg · kg-1 ·h-1 for postoperative analgesia; Group Ⅱ : sufentanil 0. 06μg · kg-1 · h-1 for postoperative analgesia; Group Ⅲ :sufentanil 0.08μg/kg · h for postoperative analgesia;PCSA started at the end of operation with catheter buried in deltoid muscle. Visual analgesia scale(VAS) ,Rasmay scale and side effects were recorded at 4,8,16,24 and 48h after operation. ResultsVAS scores decreased significantly in group Ⅱ and group Ⅲ compared with that in group Ⅰ ( all P ＜ 0.05) ;There were no differences of Ramsay scores between groups ( all P ＞ 0. 05) ;There were three cases of respiratory depression in group Ⅲ. ConclusionPCSA with sufentanil (0.06μg/kg) had exact analgesic effects for postoperative abdominal surgery patients with less adverse reactions.

Objective To investigate the expression of serum thrombopoietin (TPO) in patients with hemophagocytic syndrome (HPS) and to evaluate its clinical significance. Methods The clinical data of HPS patients from Beijing Friendship Hospital,Capital Medical University during the period from September 2008 to July 2010 were collected. They were divided into infection-associated HPS,tumor-associated HPS and rheumatological disease associated HPS according to different causes. The serum concentration of TPO in HPS patients and 26 healthy controls was measured with enzyme-linked immunosorbent assay (ELISA). Then the correlations between TPO and laboratory indicators was analyzed. Results The level of serum TPO in 26 cases of HPS was significantly lower than that of control group (P =0.001) and the variance was not statistically significant in different causes of HPS groups using ANOVA (P =0.183). The TPO levels in HPS patients showed positive correlations with serum creatinine,after we detected their TPO levels and platelets,red blood cells,hemoglobin,serum creatinine,urea nitrogen,triglyceride,fibrinogen,ferritin,NK cell activity and sCD25 levels on the same day. Conclusion TPO together with various cytokines may affect the formation of blood cells in HPS patients,and its reduction may be associated with impaired liver function. The underlying mechanism remains to be further explored.

Bacteria ; genetics ; pathogenicity ; Dental Research ; Genomic Islands ; Periodontitis ; microbiology

Objective To observe the effects of Danhong Injection in the treatment of lacunar cerebral infarction, Methods 82 patients with lacunar cerebral infarction were randomly recruited into a treatment group (n=42) and a control group (n=40). The treatment group was treated with Danhong Injection, 30ml/d for once, while the control group was treated with Danshen Injection, i.v. 20ml/day for once. Both groups were treated for 14 days. Results The total effective rate was 97.62% and 77.50% in the treatment group and the control group respectively, showing significant difference (χ~2=4.65, P< 0.05) . Conclusion Danhong Injection is better than Danshen Injection in treatment of lacunar cerebral infarction.

OBJECTIVE: To expound the research status of the seed cells in tissue-engineered articular cartilage, and explore the resource, in vitro amplification and gene modification, so as to propose the application prospect in future.DATA SOURCES: A computer-based online search of PubMed was undertaken to identify the articles about the seed cells in tissue-engineered articular cartilage published in English between January 1994 and April 2006 with the key words of "cartilage, joint, tissue engineering, stem cell, cell culture, gene modification". Other articles were searched from Tsinghua Tongfang and Vip database according to the journal and article titles.STUDY SELECTION: The data were selected firstly to choose those about the seed cells in tissue-engineered articular cartilage and eliminate those not accorded with the requirements. The full-texts of left articles were searched manually.DATA EXTRACTION: Totally 89 articles were collected, of which 34 were included and 55 were excluded.DATA SYNTHESIS: Cells for the construction of artificial cartilage tissues include autologous or homologous chondrocytes, periost and perichondrium cells, mesenchymal stem cells and embryonic stem cells, all of which could be cultured and amplified by specific culturing system in vitro, they also could be genic modified under certain condition with different strategies and methods to repair articular cartilage defects.CONCLUSION: Seed cells with their particular characteristics play an important role in the repair of articular cartilage defects with tissue engineering technique.

Paired box2 ( PAX2 ) is a transciption factor which mainly expressed in the developing kid-ney. Researches indicate that PAX2 promote the transcription through interactions with the adaptor PAX transac-tivation domain interacting protein(PTIP). Otherwise,PAX2 protein can lead to chromatin compaction and gene silencing through interactions with Grg4. PAX2 reexpressed in acute kidney injury and involved in promoting cell proliferation. Congenital PAX2 gene mutation is closely related to congenital abnormalies of the kidney and uri-nary tract. In chronic kidney disease,PAX2 promote proliferation and cyst formation. Here,the recent researches on the function of PAX2 and its role in acute kidney injury and chronic kidney disease are reviewed.

Objective:To establish the quality standard for infantile bronchitis traditional Chinese medicine lotion. Methods:TLC was used for the qualitative identification of Ephedra herba, Paeonia lactiflora Pall. , Poria cocos, Fructus aurantii and Epimedium brev-icornu Maxim. . HPLC was used for the content determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and paeoni-florin. The chromatography conditions for ephedrine hydrochloride and pseudoephedrine hydrochloride were as follows: an AElichrom Pdar-Phenyl Polyphenyl ether-bonded phenyl-bonded silica column (250 mm × 4. 6 mm, 5μm) was used, the mobile phase was aceto-nitrile-0. 2% phosphoric acid solution (1 :99), the flow rate was 1. 0ml·min-1, the detection wavelength was 210 nm, the column temperature was 30℃ and the injection volume was 10 μl. The chromatography conditions for paeoniflorin were as follows:an Inertsil ODS-3 C18 column (250 mm × 4. 6 mm, 5 μm) was used, the mobile phase was acetonitrile-0. 1% phosphoric acid solution (15:85), the flow rate was 1. 0 ml·min-1, the detection wavelength was 230 nm, the column temperature was 30℃ and the injection vol-ume was 10 μl. Results:The TLC results of Ephedra herba, Paeonia lactiflora Pall. , Poria cocos, Fructus aurantii and Epimedium brevicornu Maxim. showed clear spots with good resolution. Ephedrine hydrochloride, pseudoephedrine hydrochloride and paeoniflorin had a good linear relationship within the range of 10.11-101.10 μg·ml-1(r=0.9996), 10.08-100.80 μg·ml-1(r=0.9991) and 20.50-102.5 μg·ml-1(r =0.9996), respectively. The average recovery was 98.80% (RSD =1.87%, n =6), 98.77%(RSD=1. 72%, n=6) and 99. 57% (RSD=1. 56%, n=6), respectively. Conclusion: The established quality standard can be used for the quality control of infantile bronchitis traditional Chinese medicine lotion.

Objective To discuss different performance of DR and CT examination on secondary pulmonary tuberculosis,to improve the clinical guidance value of CT in the diagnosis of secondary pulmonary tuberculosis. Methods The image data of 52 cases with secondary pulmonary tuberculosis confirmed by pathology were collected, including chest DR radiography and CT scans.The different image characteristics of two kinds of imaging examination were analyzed and compared.Results In 52 cases of secondary pulmonary tuberculosis,chest X -ray plain film found lesions calcification in 3 cases,2 cases of bronchiectasis,pleural thickening and adhesion in 12 cases,pleural effusion in 1 case,empty cavity in 4 cases,emphysema in 1 case,the lung nodules in 5 cases.Chest CT revealed lesions calcification in 9 cases,16 cases of bronchiectasis,pleural thickening and adhesion in 40 cases,pleural effu-sion in 4 cases,empty cavity in 10 cases,emphysema in 8 cases,the lung nodules in 10 cases,6 cases of pulmonary bullae,pulmonary interstitial change:4 cases of lung reticulocyte shadows,interlobular septal thickening in 4 cases, ground glass sample density in 4 cases.Conclusion DR and CT can be used for inspection of secondary pulmonary tuberculosis,but CT is a better tool,it has important guiding role for clinical diagnosis and treatment.

BACKGROUND:Different local anesthetic solutions for tumescent liposuction are necessary and have a certain effect on the biological characteristics of human adipose stem cels.
OBJECTIVE:To explore the effect of different tumescent local anesthesia on the proliferation and differentiation of human adipose stem cels culturedin vitro.
METHODS: Passage 3 human adipose stem cels were intervened by different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine). Cels cultured in low-glucose DMEM served as controls. Proliferation ability, lactate dehydrogenase activity in the supernatant and percentage of cels under adipogenic differentiation were detected.
RESULTS AND CONCLUSION:After intervention for 1, 2, 3, 4, 5 days, the supernatant lactate dehydrogenase activity in the bupivacaine, ropivacaine, lidocaine groups was significantly higher than that in the control group (P < 0.05), and moreover, the lactate dehydrogenase activity in the bupivacaine group was significantly higher than that in the ropivacaine group and lidocaine group (P < 0.05). The absorbance value of human adipose stem cels was lower in the bupivacaine, ropivacaine, lidocaine groups than the control group (P < 0.05) as wel as lower in the bupivacaine group than the ropivacaine and lidocaine groups (P < 0.05). There was no significant difference in the percentage of cels under adipogenic differentiation between groups (P > 0.05). Overal, these findings indicate that different tumescent local anesthesia (bupivacaine, ropivacaine, lidocaine) can al inhibit the proliferation of human adipose stem cels, but have no certain effects on the adipogenic differentiation.

Objective To evaluate the precision of the ACCU-CHEK Performa glucose monitor,the Optium Xceed glucose monitor,the Breeze glucose monitor and the Contour glucose monitor.Methods A total of 102 patients (diabetic patients or un-diabetic patients) who visited our endocrinology outpatient department were randomly selected.The venous blood samples were collected using the laboratory auto-analyzer,and the synchronous finger tip capillary blood were collected using the ACCU-CHEK Performa glucose monitor and the Optium Xceed glucose monitor,or using the Breeze glucose monitor and the Contour glucose monitor.Results The correlation coefficients between VPG by the laboratory auto-analyzer and CBG by the four kinds of glucose monitors was good,and R values were 0.990,0.985,0.963,0.952,and there was no significant difference between the CBG of Breeze glucose monitor and the same VPG(P ＞ 0.05).When the blood glucose concentration was higher than 4.2mmol/L,and compared with the VPG detected by synchronous measurement laboratory,95％ of the CBG test results detected by four kinds of blood glucose meters were in the range of ± 20％.Conclusion The four kinds of glucose monitors can provide high accurate.