1.A new labdane diterpenoid glycoside from Nervilia fordii.
Gui-Kun HUANG ; Li QIU ; Yang JIAO ; Ji-Zhao XIE ; Lu-Hui ZOU
Acta Pharmaceutica Sinica 2014;49(5):652-655
To study the chemical constituents of Nervilia fordii (Hance) Schltr., various chromatographic methods were used, including D101 macroporous resin, silica gel, ODS and preparative HPLC chromatographic techniques. A new labdane diterpenoid glycoside named as nervilifordoside A was isolated from 60% EtOH extract of Nerviliafordii. The structure of compound 1 was elucidated as 12, 17-epoxy-3-hydroxy-17-methoxy-labdan-13-en-16, 15-olide 3-O-alpha-rhamnopyranosyl-(1 --> 2)-O-beta-glucopyranoside on the basis of HR-MS, 1D and 2D NMR spectroscopic data as well as chemical methods.
Chromatography, High Pressure Liquid
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Molecular Structure
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Orchidaceae
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chemistry
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Plants, Medicinal
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chemistry
2.High-Level Expression of Penicillinase Gene in Bacillus Subtilis
Hong-Kun ZHAO ; Xing-Wang LIU ; Qiang QIU ; Xiu-Xing LI ; Lian-Xiang DU ;
China Biotechnology 2006;0(12):-
To obtain a number of extracellular penicillinase,the gene(penp)encoding penicillinse of Bacillus cereus ATCC10987 was cloned into expression vector in Bacillus subtilis,transformed into Bacillus subtilis DB104 deficient in two proteases.When recombinant bacteria was cultured in LB medium for 24 hours,the result of SDS-PAGE showed that the molecular weight of the protein was 28kDa and the penicillinase activity reached 339U/ml.By screening seven different fermentation media,the result showed that 4# medium is favorable to producing penicillinase by the recombinant cells than the others,with the yield of the enzyme being 1580U/ml.When the recombinant cells was cultured in 7 liter fermentor for 24h,the penicillinase activity reached 1255.8U/ml.
3.Electronic moxibustion apparatus and traditional moxibustion in treating knee osteoarthritis: a randomized controlled trial
Kun XUE ; Qiu-Juan XING ; Ya-Qiong WANG ; Ji WU ; Hai-Yin ZHAO ; Wang LU
Journal of Acupuncture and Tuina Science 2020;18(4):286-294
Objective: To observe the clinical efficacy of DZWJY-1 type electronic moxibustion apparatus and traditional moxibustion in treating knee osteoarthritis (KOA). Methods: A total of 76 eligible patients were randomized into an electronic moxibustion apparatus group and a traditional moxibustion group, with 38 cases in each group. The electronic moxibustion apparatus group was intervened by DZWJY-1 type electronic moxibustion apparatus, and the traditional moxibustion group received moxa stick moxibustion for treatment. Neixiyan (EX-LE 4), Dubi (ST 35), Xuehai (SP 10) and Liangqiu (ST 34) were selected for both groups and the treatment was conducted 3 times a week for a total of 12 times. The visual analog scale (VAS) and the Western Ontario and McMaster Universities osteoarthritis index (WOMAC) scores were observed before treatment and after 6 and 12 sessions of treatment, respectively. Results: There were 4 dropout cases in the traditional moxibustion group. Therefore, this trial had 72 valid cases, including 38 cases in the electronic moxibustion apparatus group and 34 cases in the traditional moxibustion group, the differences in the baseline data between the two groups were statistically insignificant (P>0.05). After 6 and 12 sessions of treatment, the VAS scores decreased significantly with the increase of treatment sessions in both groups (all P<0.01), and the betweengroup differences were statistically insignificant at the same time points (both P>0.05). The pain intensity was evaluated using the weighted value of VAS score. The markedly effective rate was 47.4% and the total effective rate was 89.5% in the electronic moxibustion apparatus group, versus 50.0% and 94.1% in the traditional moxibustion group, and the betweengroup differences were statistically insignificant (both P>0.05). After 6 and 12 sessions of treatment, the total score and the component scores including pain, stiffness and difficulty moving in the WOMAC decreased significantly with the increase of treatment sessions in both groups (all P<0.01), and the between-group differences were statistically insignificant (all P>0.05). Conclusion: Electronic moxibustion apparatus and traditional moxibustion both are effective in reducing joint pain and improving joint function in KOA patients, and they are equivalent comparing the clinical efficacy.
4.Prognostic value of CD4+CD25+ Tregs as a valuable biomarker for patients with sepsis in ICU
Kun CHEN ; Qiu-Xiang ZHOU ; Hong-Wei SHAN ; Wen-Fang LI ; Zhao-Fen LIN
World Journal of Emergency Medicine 2015;6(1):40-43
BACKGROUND: Sepsis is a common complication of infections, burns, traumas, surgeries, poisonings, and post-cardiopulmonary resuscitation. The present study aimed to investigate prognostic value of CD4+CD25+ regulatory T cells (Treg) in peripheral blood of patients with sepsis. METHODS: Periphery blood from 28 patients diagnosed with sepsis was collected on day 1 and 7 after hospitalization in the ICU of Shanghai Changzheng Hospital between December 2013 to April 2014. The blood was used for analyses of Treg ratio using flow cytometry and for analyses of blood routine test, C-reactive protein (CRP), bilirubin, procalcitonin (PCT), and coagulation. APACHE II and sequential organ failure assessment (SOFA) scores were also investigated. The results were compared between two outcome groups of survival or death to evaluate prognostic value for sepsis. RESULTS: The patients had an average age of 60.36±15.03 years, APACHE II score 16.68±7.00, and SOFA score 7.18±3.78. Among the 28 patients, 12 had severe trauma (42.9%), 10 had septic shock (35.7%), and 9 (32.2%) died. The median ratio of Tregs was 2.10% (0.80%, 3.10%) in the survival group vs. 1.80% (1.15%, 3.65%) in the death group (Z=–0.148, P=0.883) on day 1; however it was significantly changed to 0.90% (0.30%, 2.80%) vs. 5.70% (2.60%, 8.30%) (Z=–2.905, P=0.004). CONCLUSION: With better prospects for clinical application, dynamic monitoring of Tregs ratio in peripheral blood has potential value in predicting prognosis of sepsis.
5.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
6.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
7.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
8.Effect of troxerutin and cerebroproptein hydrolysate injection on platelet aggregation and thrombosis.
Qiu-Chen CHEN ; Zhao-Jin YU ; Hai-Gang SUN ; Jian-Kun YU ; Min-Jie WEI
Journal of Experimental Hematology 2011;19(1):193-196
This study was purposed to explore the effect of troxerotin and cerebroproptein hydrolysate injection (TCHI) on platelet aggregation in vitro and thrombosis in vivo. The inhibitory rate of TCHI at different concentrations on platelet aggregation was determined by platelet aggregometer. The relationship between dose and effect was established. The effect of troxerutin and cerebroproptein hydrolysate injection on thrombosis was determined by the carotid thrombosis model of rats. The results showed that the TCHI could inhibit thrombosis and platelet aggregation in a concentration-dependent way. When the concentration of TCHI total nitrogen was 5 µg/ml, the inhibition rate of platelet aggregation reached to the highest value of 28.61 ± 22.07%, which is 2.5 times as much as that with 100 µg/ml aspirin. It is concluded that the TCHI has antiaggregative and antithrombotic activity effects against platelet aggregation and thrombosis.
Animals
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Hydroxyethylrutoside
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analogs & derivatives
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pharmacology
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Platelet Aggregation
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drug effects
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Protein Hydrolysates
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pharmacology
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Rabbits
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Rats
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Rats, Wistar
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Thrombosis
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prevention & control
9.Inhibitory effect of interference hTERT and TRF2 gene on the growth of breast cancer MCF-7 cells.
Shao-kun CHEN ; Lan LIU ; Qin-lin SHUI ; Hong YU ; Yong-qiu ZENG ; Jiao ZHAO
Chinese Journal of Oncology 2010;32(2):93-97
OBJECTIVETo explore the effect of combined gene therapy with interference hTERT and TRF2 gene on the treatment of breast cancer.
METHODSRecombinant adenovirus rAd-hTERT and rAd-TRF2 expressing siRNA-hTERT and siRNA-TRF2 was constructed, and the vectors were transfected into MCF-7 cells. Than the expressions of hTERT and TRF2 proteins were detected by Western blot, the inhibition of MCF-7 cell proliferation by MTT colorimetry, and the changes of MCF-7 cell cycle by flow cytometry and the colony forming ability of MCF-7 cells by clone form test.
RESULTSAt 48 h after transfection, the relative expression amounts of hTERT protein of the PBS control group, rAd-blank group, rAd-HK control group, rAd-hTERT group, rAd-TRF2 group and rAd-hTERT and rAd-TRF2 group were 1.00, 0.94 +/- 0.02, 0.95 +/- 0.04, 0.18 +/- 0.04, 0.95 +/- 0.01 and 0.18 +/- 0.04, respectively. The relative expression amounts of TRF2 protein were 1.00, 1.01 +/- 0.08, 0.96 +/- 0.02, 0.95 +/- 0.08, 0.22 +/- 0.01 and 0.26 +/- 0.02, respectively. After transfection of rAd-hTERT or rAd-TRF2 into MCF-7 cells separately, the inhibition rate of cell proliferation was only 54.6% and 48.4%, there was 8.9% +/- 1.2% or 9.2% +/- 2.3% of MCF-7 cells into M phase, 66.4% +/- 1.5% or 64.6% +/- 1.9% of MCF-7 cells was arrested at G(0)/G(1) phase, and the cell colony forming ability was decreased significantly (cell colony number from 100 in PBS control group down to 41.3 +/- 5.1 and 43.7 +/- 6.4). But after transfection by rAd-hTERT and rAd-TRF2 simultaneously, the inhibition rate of cell proliferation was about 82.1%, and M phase cells was significantly reduced to 4.4% +/- 1.2%. Large numbers of cells were arrested at G(0)/G(1) phase (81.4% +/- 1.3%), and the cell colony forming ability was more significantly decreased (cell colony number there were only 29.2 +/- 3.9).
CONCLUSIONMore effective effect of tumor gene therapy can be achieved by combination of interference hTERT and TRF2 genes as compared with interference by either of the single gene alone.
Adenoviridae ; genetics ; Breast Neoplasms ; genetics ; metabolism ; pathology ; Cell Cycle ; Cell Line, Tumor ; Cell Proliferation ; Female ; Gene Expression Regulation, Neoplastic ; Genetic Therapy ; Genetic Vectors ; Humans ; RNA Interference ; RNA, Small Interfering ; genetics ; Telomerase ; genetics ; metabolism ; Telomeric Repeat Binding Protein 2 ; genetics ; metabolism ; Transfection ; Tumor Stem Cell Assay
10.Two-year observation of a randomized trial on tacrolimus-based therapy with withdrawal of steroids or mycophenolate mofetil after renal transplantation.
Qing-guo ZHU ; Ya-kun ZHAO ; Wei LIU ; Hui LUO ; Yu QIU ; Zhi-zhong GAO
Chinese Medical Sciences Journal 2008;23(4):244-248
OBJECTIVETo evaluate the safety and feasibility of steroid or mycophenolate mofetil (MMF) withdrawal from tacrolimus-based immunosuppressant regimen in renal allograft recipients.
METHODSA cohort of 45 patients following cadaveric renal allograft transplantation were randomly divided into 3 groups based on the regimen of combination of tacrolimus, steroid, and MMF: triple therapy group, steroid withdrawal group, and MMF withdrawal group. During 2 years, survival of patients and allografts, clinical acute rejection, adverse events, hepatic and renal allograft function, and blood lipids were monitored to evaluate the safety and feasibility of steroid or MMF withdrawal after renal transplantation.
RESULTSDuring two-year observation, steroid or MMF was successfully withdrawn from immunosuppressant regimen based on tacrolimus without any clinical acute rejection. Patient and graft survival rates were 100% and all the renal allografts kept excellent function. Some adverse events occurred and there were no significant differences among groups.
CONCLUSIONWithdrawal of steroid or MMF in low-immunological-risk renal allografts treated with tacrolimus-based immunosuppressant regimen can be achieved with no increased risk of acute rejection.
Adult ; Aged ; Drug Therapy, Combination ; Graft Rejection ; drug therapy ; prevention & control ; Humans ; Immunosuppressive Agents ; therapeutic use ; Kidney Transplantation ; adverse effects ; immunology ; Lipids ; blood ; Middle Aged ; Mycophenolic Acid ; analogs & derivatives ; therapeutic use ; Prospective Studies ; Steroids ; therapeutic use ; Tacrolimus ; therapeutic use ; Transplantation, Homologous ; immunology