Objective:To investigate the inhibition of Eleo-Zedoary solution to human endometrial cancer cell line RL-952 in vitro and its possible mechanism.Methods:Using the MTT assay to display the sensitivity to the drug,the inhibition of Eleo-Zedoary parenteral solution to RL-952 cells in vitro was observed;and using light microscope and transmission electron microscope,the morphological alterations of the cells were observed.Results:The inhibition rate of Eleo-Zedoary parenteral solution to RL-952 indicated both dose-dependent and time-dependent anti-proliferative effect.Microscopically,the large vacuoles in the cytoplasm of the cells were found.Ultramicroscopically,many fat-like drops and obvious swelling of organelles as well as the mitochondrial cristaes in the cytoplasm of cells were observed.In addition the nucleus concentration and the chromatin fragmentation and coagulation,including the apoptotic bodies were observed.Conclusion:Eleo-Zedoary parenteral solution can inhibit the proliferation of human endometrial cancer cell line RL-952 by induing apoptosis and secondary necrosis.

Pharmacy is a professional subject with strong practicality and applicability,in which experimental teaching is an essential part of developping students' innovation and overall qualities. Based on the pharmaceutical experimental teaching status,some reform programs including experimental teaching measures,utilization of online teaching and optimization of evaluation manners were provided on the purpose of improving overall quality of the students and developing more comprehensive students.

Objective To evaluate the academic level and the popularity of Chinese Journal of Laboratory Medicine.Method With Chinese Medical Citation Index(CMCI), we analyzed the amount and distribution of the originals in Chinese Journal of Laboratory Medicine cited by the journals indexed in CMCI.Result The percent of cited papers in all published articles was 55.68% during the period, the average number of cited times is 4.83, the top cited value was 108.206 articles (30.03% of total papers) were cited 5 or more, and the all cited times from them were 2428(73.33% of total cited times). 686 cited articles were written by 526 authors from different countries.The most frequently cited author wrote 12 papers, the authors with one paper cited were 430(81.75% of total authors).The cited authors are from the 31 provinces and other country; Beijing, Zhejiang and Shanghai are in the front of Laboratory Medicine research.Beijing was the top of rank (44.40% of total authors).292 institutes were cited and the most frequently cited institute was Beijing Hospital(10.48% of total cited times).There were 563 citing journals where the self-citing rate is 9.85%.Conclusion The Chinese Journal of Laboratory Medicine has published high quality articles. It is the one of the most important information resource for the laboratory medicine researchers and the most important medical journal.

This paper discussed the selection of SCI journals and questions associated with submitting manuscripts.The strategy for the submitting manuscripts.and the problems in the publication of manuscripts were also summarized in this article.These results would be valuable and guide for the researchers to submit their manuscripts,conform the writing skills of scientific papers,and improve the efficiency of SCI publications.

ObjectiveTo investigate the expression and significance of CDX2,Ki-67 in adenoma and colorectal adenocarcinoma,and the correlation between CDX2 and Ki-67.MethodsThe expression of CDX2 and Ki-67 was evaluated in the colorectal adenocarcinoma,adenoma and normal colorectal mucosa as control subjects by immunohistochemistry.ResultsThe magnitude of CDX2 were negatively correlated with the level of dysplasia in adenoma(rs=-0.38,P =0.02),and negatively correlated with differentiation,lymph node metastasis,diversion and Dukes stage of cancer in colorectal adenocarcinoma(rs=-0.49,rs=-0.37,rs =- 0.38,rs =-0.37,P =0.01).The expression and relativity of Ki-67 in the samples were contrary to the CDX2.The levels of CDX2 and Ki-67 expression had significant difference between the adenoma and adenocarcinoma,and showed negatively correlation in this two type tissues (rs=-0.69,P =0.00; rs =-0.40,P =0.00).ConclusionsCDX2 may be used as an effective marker to evaluate malignancy degree and prognosis of large bowel neoplasm patient.The levels of Ki-67 expression in colorectal adenocarcinoma are correlated with malignancy degree and clinical stage.The expression of CDX2 is negatively correlated with Ki-67.Combined detection of CDX2 and Ki-67 may be helpful for the judgment of colorectal tumor biological characters.

Vascular eognitive impairment (VCI) is a large class of syndromes from mild cognitive impairment to dementia, which are caused by the cerebrovascular risk factors (eg, hypertension, diabetes and hyperlipemia), obvious (eg, cerebral infarction and intracerebral hemorrhage) or unobvious cerebrovascular diseases (leukoaraiosis and chronic cerebral ischemia). Accurate early diagnosis provides the best treatment opportunity and slows down the progress of the disease. In recent years, the research on VCI has made a great progress. This article reviews VCI from the aspects of the etiology, clinical manifestation, neuropsychology, imaging, neuroelectrophysiology, biomarkers, and diagnostic criteria.

Objective The purpose of this study is to analyse the expression of tumor necrosis factor (TNF)-related apoptosis inducing ligand(TRAIL)and its receptors in muscle tissue from patients with idiopathic inflammatory myopathies(ⅡM)and investigate the possible role of TRAIL system in the Dathogenesis of IIM.Methods TRAIL and its receptors DR4,DR5,DCRl and DCR2 were detected in the musck biopsy tissue from 36 patients with ⅡM(13 polymyositis,23 dermatomyositis)and 9 heMthy controls bv using immunohistochemistry. Results The expression of TRAIL and its receptors was f10und in muscle tissue samples from the IIM patients and healthy controls.The expression of TRAIL,DR4 and DCR2 in the muscle tissue from the IIM patients was significantly higher than those from healthy controls(all P values< 0.05).The expression of TRAIL,DCRl and DR4 was also detected in the infiltrating lymphocytes in endomysium and in the interstitial tissue around blood vessels.Conclusion The diversity in expression of TRAIL and its receptors between patients with IIM and healthy controls suggests the hypothesis of a crucial role of TRAIL in the pathogenesis and the pathology of IIM.

As a molecular switch,Rho can be transformed between the inactive GDP-bound form and the active GTP-bound form,transfering signals to downstream effector Rho kinase (ROCK),mediating specific biological effects,and participating in various neurological diseases, such as the pathological processes of ischemic stroke,cerebral vasospasm,and axonal regeneration after spinal cord injury.The application of ROCK inhibitor in the treatment of neurological diseases has achieved satisfactory effect.Rho/ROCK is expected to become a novel therapeutic target.

Objective: To investigate the relationship between the serum ferritin concentration and insulin resistance in patients with impaired glucose tolerance(IGT).Methods: The serum ferritin concentration and insulin levels were measured in 40 IGT patients(20 with normal body weight and 20 with obesity),and 40 healthy controls.Blood glucose levels,HbA1C,blood adipose and urine protein concentration were obtained simultaneously.Results: In the IGT patients,the serum ferritin concentration was correlated with Hba1c but not with any other indexes,and it was significantly higher than in the normal controls.Significant differences were observed between the two groups in all the indexes but the fasting insulin level.Conclusion: The serum ferritin concentration increases in IGT patients,which implicates some association of ferritin with glucose metabolism and insulin resistance.

Objective To provide references to NSF funding policy and planning based on the grant project analysis.Methods Funding grants of NSF Distinguished Young Scholars in medical field during 2011-2015 were collected,and compared with the data of 2001-2010.Indicator data was analyzed including grant distribution in supporting institutes,geography locations and medical subject.Results In recent 5 years,the number of application for the grant is increasing,while the funding number stays the same.The universities governed by Ministry of Education and China Academy of Science are the primary grantee institutions.Beijing,Shanghai,and other developed areas got more grants than other provinces.Most grants were focused on oncology,neurology and psychology,medical imaging and medical engineering,and pathogen biology and infection.Conclusions The funding number of NSF supporting for Distinguished Young Scholars should be incresed with applications increasing.The supporting covers in areas,institutes and medical subjects should be extensively.