Objective The invasion and metastasis of tumor cells depend on the growth of new blood vessels, and tumor neovascularization was regulated by a lots of factors. This study aimed to investigate the expression of hypoxia-inducible factor-1α (HIF -1α) and vascular endothelial growth factor (VECF) in retinoblastoma and explore the correlation of HIF-1α and VEGF with tumor invasion and metastasis and analyze their relationship with clinicopathology characters and determine their effect on angiogenesis. Methods Forty-six retinoblastoma specimens with different clinical stages were collected in Affiliated Second Hospital of Nanchang University. The specimens received neither radiotherapy nor chemotherapy. The retinal tissue near the tumor was as control. Immunohistochemistry was used to detect the expression of HIF-1α protein and VEGF protein in retinoblastoma. The relationship between expression of HIF-1α and VEGF and stage of tumor was analyzed. Results The HIF-1α and VEGF were highly expressed in the ischemia or necrosis area of retinoblastoma. Expression of HIF-1α was mainly in cell nuclear and partly in cytoplasm, and VEGF was mainly expressed in cytoplasm of tumor cells. The expression of HIF-1α and VEGF was gradually increased with the rising of tumor stage (P ＜ 0. 05) and showed significant correlation between expression of HIF-1α and VEGF with tumor stage(r_s =0. 943, P ＜0. 01). HIF-1α exepression was also possitively related to VEGF level in retinoblastoma (r, =0. 946, P ＜ 0. 01). Conclusion HIF-1α and VEGF were over-expressed in retinoblastoma cells, and the expressions were related to clinical staging, invasion and metastasis of tumor cells. The expression of HIF-1α and VEGF was one of the predictors for the biological behaviors of retinoblastoma. HIF-1α and VEGF may play an important role in angiogenesis and tumor progression in retinoblastoma.

Objective To recover laboratory data and software function in machine-number-based encryption. Methods Database and cryptograph files were replaced respectively by the corresponding files backuped previously after the operating software of Tecan SunRise setting up in new system,and set essential configuration. Results Laboratory data and software function were recovered entirely,and apparatus ran normally. Conclusion Besides database files,the cryptograph files were also essential data, which must be preserved in machine number based encryption.

Congenital Abnormalities ; epidemiology ; Hospitals ; statistics & numerical data ; Humans ; Population Surveillance ; methods

OBJECTIVETo investigate the therapeutic effect of scalp expanded flap combined with JPL hair removal for large area scar on forehead.

METHODSFrom Jul. 2010 to Nov. 2012, 9 cases with large area scar on forehead received treatment of adjacent scalp expanded flap combined with JPL hair removal. One the first stage, the expander was implanted under the scalp near the forehead scar, followed hy expansion process. When the expansion was completed, the expanded flap was transferred to cover the wound after scar excision. 10 days after flap transposition, the forehead hair line was designed and extra hair on flap underwent JPL hair removal. After 3-5 treatments, the hair on flap was almost removed.

RESULTSAll the scalp flaps survived completely. JPL hair removal had exact effect. The patients were followed up for 5-11 months. The hair density on the flap decreased hy more than 90%. The flap had a good match with surrounding facial skin in color, texture and thickness. The reconstructed forehead hair line was satisfactory.

CONCLUSIONSIt is a good option to reconstruct large area scar on forehead with scalp expanded flap comhined with IPL hair removal.

Cicatrix ; surgery ; Forehead ; injuries ; surgery ; Graft Survival ; Hair Removal ; methods ; Humans ; Scalp ; Surgical Flaps ; transplantation ; Tissue Expansion ; methods ; Tissue Expansion Devices

A series of 2-methoxyestradiol (2-MeO-E2) RGD peptide conjugates with coupling RGD peptides to 3- position or 17-position of 2-MeO-E2 through space linker were synthesized. Their antiangiogenic properties were preliminarily evaluated by cell migration scratch assays against HUVECs. Compound 26c binding RGDV peptide showed the best inhibitory effect. In addition, all 2-MeO-E2 RGD peptide conjugates exhibited obvious activity. These results demonstrate that conjugates with RGD peptides represent a promising means for targeting angiogenesis in cancer therapy.

Objective To evaluate the effect of three diamension-digital subtraction angiography (3D-DSA) or computed tomography angiography (CTA) on the patients with ruptured multiple intmcranial aneurysm (MIA). Methods A retrospective study on 21 patients with MIA was performed.After scanning with 3D-DSA or 3D-CTA, three-dimensional reconstruction of MIA was carried out by 3D workstation,then the diagnosis was decided and the treatment plan (endovascular treatment or microsurgery) was selected according to stereoscopic image of MIA. Results (1) 3D-DSA or CTA was performed in 21 patients with subarachnoid hemorrhage (SAH),it was revealed these patients carried with 48 aneurysms,including 35 small aneurysms (25 mm).Not only miero-aneurysms and small aneurysms could be precisely showed,also the size of aneurysmal neck,the relationship of the aneurysm and the parent vessel and contiguous branches by stereoscopic image.(2) According to the standard of classification,9 patients with MIA for gradeⅠ(42.9%),10 for gradeⅡ(47.6%),2 for gradeⅢ(9.5%),0 for gradeⅣ.Endovascular treatment was selected prior to microsargery for those high grade patients.In this group,17 patients with 40 aneurysms underwent endovascular embolotherapy with GDC coils.Twenty four anemysms were completely occlusioned,12 beyond 90%,4 were left without treatment because of their small size.In microsurgery group,3 aneurysrus were totally clipped,1 could not be found during operation.No any treatment was accepted in 2 patients with 4 aneurysms. Conclusions 3D-DSA or CTA,which is very useful for the diagnosis and treatment of MIA,could improve the accuracy of diagnosis of MIA and clearly show the stereoscopic image of MIA,also the relation of sac and parent artery.For those patients with high grade MIA,endovascular treatment was selected prior to microsurgery,pro re nata,used to combine with mierosurgery.

Objective To investigate the processing characteristics of locative prepositions in patients with Chinese aphasia,and to provide the theoretical evidence for the rehabilitation of aphasia.Methods Twenty aphasic patients caused by left-hemisphere stroke and twenty matched normal controls were studied.Using the locative prepo- sition repeating task(single words,locative preposition phrases and words in sentences),the comprehension task, filling-gap task,the visual-spatial function task and the short-term memory task,we compared the performance be- tween these two groups.Results The aphasic patients had more difficulty in repeating locative prepositions in sen- tences,in comprehension task and filling-gap task,their short term memory was impaired.Both groups did well in re- peating single words and phrases.Conclusion The processing of locative prepositions was impaired in Chinese aphasics.The repetition of locative prepositions was more difficult than that of phrases and single words.The preposi- tions were often omitted.It might be due to the impairment of their short-term memory,or it might have something to do with role they played in the syntactic structure.The latter might also impact the comprehension and filling-gap score.We should make plans before rehabilitation therapy.

In this paper, three spoilage organisms were separated from five transmutative soy milks, and all the three spoilage bacteria could survive condition of both 1?105Pa,30min and 300mg/kg Nisin. Morpha character, physiological and biochemical characteristics, and a phylogenetic analysis based on 16S rDNA gene sequences reveal that these three strains are Bacillus licheniformis, Bacillus pumilus and Brevibacillus borstelensis respectively. GenBank accessions for these three strains are EF439666-EF439668。

0.05)between the two groups.Conclu- sion The long-term outcomes of e-CHB is not markedly different compared with that of e+CHB.

0.05).However,the shorter the history of the disease,the better the efficacy of the treatment.The younger the patient was,the better the efficacy of the treatment.Conclusions Vulva dystrophy can be treated with focused ultrasound effectively and safely.This approach appears to be a new promising treatment method.