Objective To explore the prognostic value of 24 h ambulatory blood pressure monitoring (ABPM)for target organ damage (TOD) in the elderly hypertension patients. Methods Two hundred and thirty two elderly hypertension patients who were involved at least one target- organ damage experienced 24 h ABPM. All subjects were randomly divided into 3 groups according to the number of TOD and the clinical situation of cardiovascular, cerebrovascular and renal complications. 24 h ABPM recordings of each group were compared with one another. Results The number of TOD were highly correlated with 24 h average systolic pressure, nighttime average diastolic pressure, abnormal circadian rhythm and pressure burden. The difference was significant between patients involved 3 TOD and those with 1 TOD(P

BACKGROUND: Sonography is believed by many scholars to be sensitive for the detection of hepatic arterial thrombosis and stenosis, but it is difficult to show the anastomotic vascular stenosis. MR angiography is better than sonography in the display of vascular anatomy and the diagnosis of various vascular complications after liver transplantation.OBJECTIVE: To evaluate the role of MR angiography (MRA) in the display of vascular anatomy and the diagnosis of various vascular complications after liver transplantation.DESIGN: Contrast trial observation.SETTING: Medical Imaging Center of Nanfang Hospital, Southern Medical University.PARTICIPANTS: Eleven adult male patients had undergone liver transplantations at Nanfang Hospital of Southern Medical University between January 2004 and December 2006. They ranged in age from 40 to 58 years, average 49 yeas. Original causes of liver failure in the study group included cirrhosis (n =9) and primal hepatic carcinoma (n =2) diagnosed by pathohistological methods. And 9 cases had undergone orthotopic liver transplantations while 2 cases with piggy-back liver transplantation.METHODS: Eleven consecutive adult patients underwent MR imaging examinations after orthotopic liver transplantation using a breath-hold 2D True Fast Imaging with Stead-state Precession and Fast Low Angle Shot. MR triphasic contrast-enhanced 3D imaging was also performed. Enhancement scan: A final gadolinium-enhanced axial and coronal T1WI spin-echo sequence with spectral fat saturation was performed after completion of the MRA. The vascular diameter stenosis was calculated according to S (S=[(D-d)/D]×100%) by ECST method, d as the inner diameter of the most obvious stenosis while D as normal diameter. Degree of stenosis: normal as S 0-30%, mild stenosis as S 31%-50%, moderate stenosis as S 51%-85%, and severe stenosis as S 86%-100%. Then the imaging findings after liver transplantation were analyzed. Meanwhile the sonography was performed.MAIN OUTCOME MEASURES: ① MR Image analysis of vascular anatomy and vascular complications after liver transplantation.②Normal findings after liver transplantation by using sonography.RESULTS: All 11 patients were involved in the result analysis.①MRA: The anastomosis of hepatic artery, portal vein and inferior vena cava were smooth in 3 cases. The high signal intensity was seen around portal vein at T2WI in 1 case with a shorter interval after transplantation and persisted 3 weeks. In 1 case, the caliber of the donor portion was smaller than the recipient portion. Among other 8 cases, hepatic artery complication included hepatic artery winding in 2 cases and aneurysm formation of donor's hepatic artery in 2 cases, but the twig of the hepatic artery was normal. Mild Portal vein stenosis at the anastomosis was found in 2 cases, caused by the different calibers of the donor portion from the recipient portion, but the inter-hepatic branches of the portal vein were normal. Clubbed dilatation of hepatic veins end-brush was depicted in 2 cases whose inferior vena cava at the anastomosis was not stenosis. Inferior vena cava thrombosis was found in 1 case. The thrombus displayed the low signal intensity in the high signal intensity of inferior vena cava. By follow-up examination, the degree of clubbed dilatation of hepatic veins end-brush reduced after half a year and inferior vena cava thrombosis disappeared by treatment. ②Sonography: By ultrasound examination, 1 case who had hepatic artery winding combining to aneurysm formation of donor's hepatic artery was discovered just hepatic artery winding. In 2cases that had mild portal vein stenosis at the anastomosis, 1 case was diagnosed normal while the other was not affirmed. The clubbed dilatation of hepatic veins end-brush was not depicted. The others were same as MRI diagnosis.CONCLUSION: Dynamic enhanced 3D MRA imaging can provide a comprehensive assessment of vascular anatomy in most recipients of liver transplants, and is an accurate and quick method to diagnose the vascular complication after liver transplantation.

Objective ABCG2(ATP-binding cassette superfamily G member 2)is a member of ABC transporter superfamily,and participates in the mechanisms of high resistance to chemotherapeutic agents in tumor treatment.The aim of present study is to investigate the expression and significance of ABCG2 in lung cancer tissues.Methods Lung cancer tissues were obtained from 83 patients(64 NSCLC,19 SCLC)by bio-autopsy or surgery.No patients had received chemotherapy or radiotherapy before bio-autopsy or surgery.83 lung cancer specimens were analyzed for ABCG2 protein expression by using immunohistochemistry.Negative controls had the primary antibody eliminated.Human placenta tissues were used as positive control.In addition,five specimens of normal lung tissues were included for comparative study.Results The present study confirmed the predominant localization of ABCG2 transporter in plasma membrane.Some of ABCG2-positive tumors showed mixed membranous and cytoplasmic staining.ABCG2 expression was found in 71.9%(46/64)of NSCLC and 10.5%(2/19)of SCLC.The level of ABCG2 expression was significantly higher in NSCLC than in SCLC(P

Objective:To study the sintering character of calcium-deficient apatite.Methods:Calcium-deficient apatite synthesised by wet chemical method was sintered at different temperature and then its chemical composition, phase structure and morphology were examined by FT-IR spectroscopy, X-ray diffraction (XRD) and field emission scanning microscopy (FESEM) respectively.Results:When the heating temperature is below 500 ℃, apatite crystal kept a stable size with a diameter of 12-26 nm and a length of 30-66 nm. After sintered at 600 ℃ for 2 h, apatite crystal were with a diameter of 25-40 nm and a length of 75-100 nm. Around 800 ℃ for 2 h, apatite was decomposed into Ca_3(PO_4)_2.The crystal size of the Ca_3(PO_4)_2 surpassed 200 nm in diameter and length. NH_4+ ion could be removed at temperature beyond 300 ℃.Conclusion: Calcium-deficient apatite has it's specific sintering character.

Objective:To study the effect of injection bovine retinal soluble antigen (S-Ag)into anterior chamber(AC)in inducing immunological tolerance.Methods:S-Ag was extracted from fresh bovine retina and inoculated into the AC of Wistar rats,One week later,the S-Ag mixed with complete fruend's adjuvant(CFA)was injected into footpad intradermaly.The clinical symptoms and the pathological changes were observed.The IL-4 and IFN-? concentrations in the supernatant of splenic cell culture were measured by ELISA.Results:The incidence of EAU in pretreated group(group A) was markedly higher than that of positive controls(gropu B).There were histopathological changes in all rats with clinical symptoms.The IL-4 level of splenic cell culture in group A were significantly higher than those in group B ( P

Objective To observe the effect of Exendin-4 on the apoptosis of cardiomyocyte induced by H2O2,and approach the relationship between GLP-1 signal pathway and the injury of cardiomyocyte.Methods Cardiomyocytes were isolated and cultured,and were divided into 5 groups.Intercelluar ROS (reactive oxygen species) was measured,and cell apoptosis rate was evaluated by Flow cytometry in different groups.Also expressions of apoptosis-associated proteins (caspase-3) and PI3 K/AKT were evaluated by western blot.Results Compared with H2O2 group,Ex-4 co-incubation decreased the production of intercelluar ROS levels,also improved the cardiomyocyte apoptosis.At the same time,Ex-4 resulted in the alterations in expressions of the caspase-3 and p-AKT/AKT proteins.However,these effects of Exendin-4 were counteracted significantly by the co-incubation of LY294002.Conclusion The interventions of GLP-1 signal pathway can improve cardiomyocyte apoptosis induced by H2O2 incubation,and the mechanisms might partly attribute to the PI3K/AKT system.

Objective To understand the characteristics of common causes of acute pancreatitis (AP) in China and evaluate the association of the aetiology with gender and mortality.Methods The relevant literature was searched by the China journal full-text database (CNKI),Wanfang database,Weipu database and other databases and proceeding.Based on collecting data retrieval strategy,according to the inclusion criteria selection literature,Meta analysis was performed mainly for gallstone,hyperlipidemia,alcohol and other AP from the aspects of gender and case fatality rate.Results The Meta analysis included 11 articles which were accordance with the criteria,involving 13 601 patients,including 6 732 cases of biliary AP,1 372 cases of hyperlipidemia AP and 1 169 cases of alcohol AP.The Meta analysis showed that biliary AP male to female ratio was 0.79 ∶ 1,hyperlipidemic AP male to female ratio was 1.54 ∶ 1,alcoholic AP male to female ratio was 10.47 ∶ 1,overeating AP male to female ratio was 1.29 ∶ 1,and there was significant difference (P ＜ 0.05).Alcoholic AP mortality rate was the highest,which was 2.81 times than the biliary AP and 2.46 times than the hyperlipemic AP,and there was significant difference (P ＜0.05).Conclusions Biliary tract disease is the main etiologic cause of AP in China,and there are more females than males.The mortality rate of alcoholic pancreatitis is the highest,and there are more males.But we should investigate further high-quality,large-scale trails in patients with AP.

Objective:To compare the titanium-porcelain bonding strength between CAD/CAM and cast pure titanium.Methods:Pure titanium samples were prepaired by CAD/CAMand casting respectively and fused with porcelain.The bond strength between tita-nium and porcelain of the samples was measured with three-point test.The titanium-porcelain interface was investigated under scanning electronic microscope(SEM).Results:The bond strength of CAD/CAMpure titanium to porcelain was (35.95 ±3.74)MPa and cast pure titanium to porcelain was (28.37 ±1 .98)MPa(P <0.05).SEMobservation showed that there was thin transition layer between titanium substrate and ceramic in CAD/CAM bond interface,titanium and porcelain combined closely,no obvious pores.However, there was thicker transition layer and small pore in cast pure titanium to porcelain bonding interface.Conclusion:The bonding strength of porcelain to CAD/CAMpure titanium is greater than that of porcelain to casting pure titanium.

Objective To explore the nuclear factor-kappa-B activator 1 (Act1) expression in salivary gland and peripheral blood lymphocytes in patients with Sj(o)gren's syndrome (SS),and to explore its possible regulatory mechanisms.Methods The Act1 expression in salivary gland tissue from 10 patients with SS was analyzed by immunohistochemical staining,and immunolluorescence double staining was used to detect Act1-expressing cells.Peripheral blood mononuclear cells were obtained from 28 SS patients and 22 healthy controls (HC).The Act1 expression in different lymphocyte subpopulations was determined by flow cytometry and Act1-mRNA expression was detected by reverse transcription polymerase chain reaction (RT-PCR),and the data was analyzed by Wilcoxon test and Mann-Whitney U test and Pearson correlation analysis.Results Remarkable expression of Actl in the salivary glands from SS patients was observed,especially in T lymphocytes,in contrast to the few expression in B lymphocytes.The expression of Act1 in the peripheral CD4+ T cells,CD8+ T cells,CD20+ B cells was higher in SS patients than HC.There was no significant difference of Act 1-mRNA levels in peripheral blood mononuclear cells in healthy controls and SS patients.However,a tendency of up-regulation of Act1 expression was noticed in SS patients than healthy controls (0.84±0.35 vs 0.79±0.21,U=274.5,P＞0.05),and the up-regulation of Act1 was also found in those SS patients with hypergammaglobulinemia (0.88±0.39 vs 0.75±0.27,U=73.0,P＞0.05) or with internal organ involvement(0.92±0.38 vs 0.77±0.33,U=75.0,P＞0.05),but the differences were not statistically significant.Conclusion Our data suggest that Act1 may be involved in the pathogenesis of SS by negative feedback mechanism in B cell-mediated humoral immune pathway,and positive feedback mechanism in IL-17 mediated T-cell immune pathways.Act1 may be associated with disease activity and severity in SS.

Objective To investigate the relationship between apolipoprotein E (ApoE) ε4,ε2 alleles and intrauterine growth.Methods ApoE genotypes of 1418 people born in Peking Union Medical College Hospital were analyzed,allele frequencies were calculated and their parameters at birth were collected.To compare ApoE ε4,ε2 alleles with parameters at birth through single factor analysis and Logistic regression analysis.Results The alleLic frequencies of e2,83 and 84 were 8.11%,83.39% and 8.50% in the group.The results of single factor analysis showed that there was significant difference between the distribution of ponderal index (PI) in the ApoE ε2 allele group(χ2=4.87 ,P=0.027).While there was no significant difference between the distribution of head circumference at birth,placental weight and gestational age in the ApoE ε2 allele group.ApoE ε2 allele showed negative correlation with small PI in the logistic regression analysis(χ2=5.077 ,P=0.024),after adjusted for gender,age,head circumference at birth,placental weight,gestational age,parity and maternal age at delivery.No association between ApoE ε4 allele and parameters at birth was found.Conclusions ApoE ε2 allele may have protective effect on PI.No association was found between ApoE ε4 allele and intrauterine growth.