Animals ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Glomerulonephritis ; drug therapy ; pathology ; Humans ; Kidney Glomerulus ; pathology ; Phytotherapy ; Podocytes ; pathology

AIM: To verify the neuroprotective effect and optimize the therapeutic dose and time window of picroside Ⅱon cerebral ischemic injury in rats .METHODS:The forebrain ischemia model was established by the method of bilateral common carotid artery occlusion ( BCCAO ) .The successful model rats were randomly divided into 16 groups according to orthogonal design and treated by intraperitoneal injection of picroside Ⅱat different ischemic time poinis and different doses .The changes of the nerve fiber myelin were observed by fast green staining .The immunohistochemical assay and Western blotting were used to quantitatively and qualitatively determine the expression of myelin basic protein (MBP). The mRNA level of MBP in the brain tissues was tested by reverse transcription polymerase chain reaction (RT-PCR).RE-SULTS:Picroside Ⅱ increased the expression of MBP and decreased demyelination after cerebral ischemic injury .The best therapeutic time window and dose were:(1) ischemia for 2.0 h with picrosideⅡat dose of 10 mg/kg according to the results of fast green staining;(2) ischemia for 2.0 h with the dose of 10 mg/kg according to the results of immunohisto-chemical assay;(3) ischemia for 2.0 h with the dose of 10 mg/kg according to the analysis of Western blotting;(4) is-chemia for 1.5 h with the dose of 20 mg/kg according to the detection of RT-PCR.CONCLUSION:Given the principle of the lowest therapeutic dose with the longest time window , the optimized therapeutic dose and time window for rat cerebral ischemic injury is intraperitoneal injection of picroside Ⅱat the doses of 10~20 mg/kg and the time window of ischemia for 1.5~2.0 h.

With Jianyu (LI 15), Jianliao (TE 14) and Jianzhen (SI 9) for instance, the three acupoints used to treat shoulder bi syndrome by needle knife, and through traceability and researching constant structure, the acupoint position, insertion trace, manipulation and clinical significance of needle knife medicine were discussed. Accurate position is one of the characteristics of acupoints selection of needle knife medicine. As for the acupoints selection method, the mean of body surface localization is always used. The phanerous or palpable bone processes, muscles and tendons are taken as positioning marks; pressing areas where appear sour, numb, or distensible and other sensations is considered as the principle of press positioning. So acupoints position method is the combination of observation and palpation. Different insertion methods can effectively relieve the accretive bursae synovialis, tendon, joint capsule and the compressed nerve, so that shoulder bi syndrome is relieved.
Acupuncture Points ; Acupuncture Therapy ; instrumentation ; methods ; Humans ; Needles ; Periarthritis ; therapy

Objective:To investigate the effect and the mechanism of T cell vaccination in inducing heterologous antigen specific immune tolerance Methods:T cell vaccination were made from the spleen cells of SD rats, which were induced by ConA and were challenged with the spleen cells of guinea pig (the heterologous specific antigen) Normal SD rats were vaccinated intraperitoneally with TCV or 1640 culture buffer(blank control) respectively One way mixed lymphocyte reaction(MLR)?the apoptosis of peripheral T cells and the rate of CD4/CD8 in peripheral blood were assayed before and after vaccination.Results:MLR showed that the response captivity of SD rat spleen cells in TCV group were suppressed significantly after vaccination(P

Adolescent ; Child ; Child, Preschool ; Female ; Herpesvirus 4, Human ; Humans ; Infant ; Infectious Mononucleosis ; diagnosis ; pathology ; virology ; Liver ; pathology ; Male

Objective To investigate the outcomes of surgery combined with albendazole liposomes in the treatment of advanced hepatic alveolar echinococcosis.Methods This is a retrospective study on 71 patients with advanced hepatic alveolar echinococcosis treated from January 2002 to November 2013.The patients were divided into 4 groups according to the surgical methods and whether surgery was combined with albendazole liposomes.Results On follow-up from 5 ～ 44 months,the mortality rates for the volume-reduction surgery group was 82.3％,the volume-reduction surgery + albendazole group 52.3％,the radical operation group 28.5％,and the radical operation + albendazole group 21.1％.The mortality rate of the radical operation + albendazole group,when compared with the radical operation group and the volume-reduction surgery + albendazole group was significantly different (P ＜ 0.05).The mortality rate of the radical operation group,when compared with the volume-reduction surgery + albendazole group showed no significant difference (P ＞ 0.05).The mortality rate of the volume-reduction surgery group,when compared with the volume-reduction surgery + albendazole group,the radical operation group,and the radical operation + albendazole group was significantly different (P ＜ 0.05).Conclusions The treatment of radical operation combined with albendazole liposomes for advanced hepatic alveolar echinococcosis reduced recurrence,improved quality of life and prolonged survival.It is a feasible method to treat these patients.

BACKGROUND:The coagulation function of patients after joint replacement is enhanced during the perioperative time, the coagulation disorder can easily lead to the deep vein thrombosis, which wil seriously affect the rehabilitation and prognosis of patients. The embolus fal ing off from venous thrombosis can lead to acute pulmonary embolism, severe cases can be life-threatening. So the early diagnosis of postoperative deep vein thrombosis and acute pulmonary embolism is very important.
OBJECTIVE:To review the research progress of clinical diagnosis of thrombosis in the perioperative patients after orthopaedic joint replacement.
METHODS:A computer-based retrieve in PubMed database and CNKI database were conducted by the first author for the articles on the clinical diagnosis of thrombosis in the perioperative patients after orthopaedic joint replacement from January 2008 to May 2013 with the key words of“arthroplasty, deep venous thrombosis, pulmonary artery embolism, risk factor, diagnostic approach, anticoagulant, perioperative period, research progress”in English and Chinese. A total of 165 articles were screened out, and final y 50 articles were included according to the inclusion criteria.
RESULTS AND CONCLUSION:After joint replacement surgery, various risk factors were associated with the etiology and pathogenesis of deep venous thrombosis, such as vascular and tissue impairments, limb fixation, pain stress, and hemorrhagic fluid caused coagulation disorder, were the main reasons to thrombosis. Deep vein thrombosis and pulmonary embolism had variety of clinical manifestations, many diagnostic approaches were widely applied in clinic, but each one has its laminations. So the positive diagnosis intervention should be performed according to the common clinical manifestations, general y begin from the routine examinations of ultrasound and electrocardiogram, and the combination of various methods was preferred if necessary in order to increase the positive diagnosis rate to the maximum extent, and take drug intervention immediately after diagnosis to avoid the happening of adverse events. Several new types of oral anticoagulants appear in clinical trials, and the outcomes are very promising, but the widely clinical application needs further observation.

Objective To compare the efficacy of different does of remifenanil combined with propofol for colonoscopy.Methods Ninety ASA Ⅰ or Ⅱ patients,aged 35-63 yr,weighing 45-72 kg,scheduled for elective painless outpatient colonoscopy,were randomly divided into 3 groups (n =30 each):remifentanil 0.2 μg/kg group (group Ⅰ),remifentanil 0.5 μg/kg group (group lⅡ) and remifentanil 1.0 μg/kg group (group Ⅲ).In groups Ⅰ-Ⅲ,remifentanil 0.2,0.5 and 1.0μg/kg were injected over 60 s,respectively,and then propofol 1.0 mg/kg was injected intravenously for induction.The colonoscope was inserted after loss of eyelash reflex.When body movement appeared during examination,remifentanil 0.1 μg/kg + propofol 0.5 mg/kg,remifentanil 0.25 μg/kg + propofol 0.5 mg/kg,and remifentanil 0.5μg/kg+ propofol 0.5 mg/kg were added in groups Ⅰ,Ⅱ and Ⅲ,respectively.The operation time,induction time,emergence time,examination room discharge time,and body movement,hypotension,bradycardia,hypoxemia and respiratory depression during operation were recorded.Results There was no significant difference in the operation time,induction time,emergence time,and examination room discharge time between the three groups (P ＞ 0.05).Compared with group Ⅰ,the incidence of body movement was significantly decreased,and the incidences of hypotension and respiratory depression were increased in group Ⅱ,and the incidence of body movement was significantly decreased,and the incidences of hypotension,hypoxemia,bradycardia and respiratory depression were increased in group Ⅲ (P ＜ 0.05).The incidences of hypoxemia,bradycardia and respiratory depression were significantly higher in group Ⅲ than in group Ⅱ (P ＜0.05).Conclusion The optimum dose of remifentanil is 0.5 μg/kg when combined with propofol for colonoscopy.

Objective To evaluate the capability for detecting of tumor cells in ascites and pleural effusion by Sysmex XN‐2000 hematology analyzer .Methods Determination of 84 samples of ascites and pleural effusion specimens with Sysmex XN‐2000 hema‐tology analyzer humoral model to analyze the study parameters highly fluorescent cells absolute value (HF‐BF # ) and percentage (HF‐BF% ) of ascites and pleural effusions screening of tumor cells ,and related data for statistical analysis .Results In 84 cases of pleural effusion and ascites samples ,cytology found 14 cases of malignant cells ,cancer cells are not found in 70 cases .14 cases of malignant cases ,HF‐BF # average of 169 /μL(0-2 001) ,HF‐BF% with an average of 29 .7% (0% -261% );70 cases of benign cases ,HF‐BF # an average of 39 /μL(0-524) ,HF‐BF% with an average of 4 .2% (0% -27 .8% ) ,both groups were statistical differences in the mean significance(P<0 .05) .ROC curve analysis with high absolute value and percentage of fluorescent cells to tumor cells detection function ,HF‐BF # area under the curve(AUC) was 0 .493 ,the area(AUC)% HF‐BF under the curve was 0 .222 .Conclusion Sysmex XN‐2000 Pattern of humoral parameters of HF‐BF # and HF‐BF% ,although the tumor cells may pro‐vide some screening information ,but ineffective ,so we believe that in our daily work can′t be over‐reliance on the instrument HF‐BF# ,HF‐BF% and other parameters should be performed on every sample smear staining ,thus improving the detection rate of ma‐lignant cells .

Objective To develop Z-score reference ranges for tricuspid annular plane systolic excursion(TAPSE) in normal fetuses from the measurements of gestational age(GA),biparietal diameter (BPD) or femur length(FL) using fetal echocardiography.Methods A retrospective cross sectional study of 1012 singleton normal fetuses were performed.The gestation age ranged from 20 to 41 weeks.Non-cardiac fetal biometric parameters included GA and BPD and FL were measured and calculated GA based on menstrual age.TAPSE was measured in a standard apical four-chamber view by free angle M-mode echocardiography.Normal Z-score ranges were developed for TAPSE using GA,BPD and FL as independent variables.These were accomplished by using first standard regression analysis and then weighted regression of absolute residual values for each parameter in order to adjust for inconstant variance.Results Linear regression model was the best description of the data in each case and correlations between TASPE and independent variables (GA,BPD,FL) were excellent.Heteroscedasticity of standard deviation (SD) with increasing independent variables also could be modeled with a simple linear regression.According to these equations,TAPSE Z-score =(the actual measurement of TAPSE-predicted TAPSE)/prediction SD.Conclusions Normal reference ranges and Z-scores for TAPSE have been provided.These normative data may be useful tools for assessment of fetal TAPSE,to evaluate fetal right ventricular function more accurately and effectively.