BACKGROUND:Drug eluting stents and endothelium stents for clinical treatment of vascular stenosis can lead to delayed endothelialization and restenosis. A rapamycin eluting stent combined with CD34 antibody can play a synergistic role to offset delayed endothelialization and intimal hyperplasia due to antiproliferative drugs, but it is stil in the pilot phase. OBJECTIVE:To observe the ability of rapamycin eluting stent combined with CD34 antibody to capture endothelial progenitor cels, and to observe the differentiation characteristics of the captured cels. METHODS:Scanning electron microscope and indirect immunofluorescence were used to observe the morphology and differentiation characteristics of captured endothelial progenitor cels. Under a fluorescence microscope, we observed the captured endothelial progenitor cels and the degree of endothelialization after implantation of the rapamycin eluting stent combined with CD34 antibody into rabbit ear vein. RESULTS AND CONCLUSION:Under the scanning electron microscope, fusiform-like cels with a diameter of 6-8 μm were captured by the composite stent, and 24 hours later, the cels became ful-shaped. The captured cels had the appearance characteristics of endothelial progenitor cels. Results from indirect immunofluorescence observation showed that there were a lot of red fluorescent spots on the coating which represented adherent cels positive for vascular endothelial growth factor receptor-2; the composite stent was largely covered with vascular endothelial cels at 24 hours after stent implantation, and fuly covered at 48 hours, but there was no abnormal cel cluster. These findings indicate that the rapamycin eluting stent combined with CD34 antibody can be specific to rapidly capture endothelial progenitor cels in the peripheral blood, and the stent can be completely covered with vascular endothelial cels at 48 hours after stent implantation, thereby achieving rapid endothelialization and promoting the repair of endothelial cels.

The content of total alkaloid in the seed of Peganum harmala L. was determined by bro-mophenol blue colorimetry. It could be considered that this is a specific, quick and simple method for thedetermination of total alkaloid in the seed of P. harmala.

Objective To investigate the genetic features related to drug-resistance of a strain of pan-drug resistant Acinetobacter baumannii. Methods The susceptibilities to 15 antibacterial agents were detected in Acinetobacter baumannii by K-B paper diffusing method. A strain of pan-drug resistant Acinetobacter baumannii was selected. The drug-resistant genes of the selected strain were amplified by polymerase chain reaction (PCR) , including 15 extended-spectrum β-lactamase genes, 3 metal β-lactamase genes, 2 AmpC enzyme genes, 7 aminoglycoside modifying enzyme genes and the genetic markers of integron I and transposons. Results The strain was resistant to 14 antibacterial agents except cefoperazone sodium/ sulbactam. β-lactamases genes ( TEM, OXA-23 and ADC) , aminoglycosides modification enzyme genes [aac(3)- I , aac(6')- I b and ant(3")- I ] , integron I qacEAi-sull and transposon tnpU were positive in PCR amplification. A new subtype of AmpC (chromosome) desoxyribonucleic acid ( DNA) was detected. Conclusion Acinetobacter baumannii is of high resistance to most antibacterial agents, and the mechanisms of the resistance are complex.

0.05).[Conclusion]The stability of the single - level anterior cervical intervertebral decompression and fusion with plating Uniplate is satisfactory.Uniplate is valuable in clinic,furthermore,it is relatively simple to operate.

Objective To study the changes of plasma copeptin level in pneumonia patients and to explore the correlation between copeptin and disease severity,outcome as well as stress hyperglycemia.Methods A total of 45 patients with severe pneumonia were included in a prospective observation.The plasma levels of copeptin,CRP and blood glucose were measured after admission,and the APACHE Ⅱ scores were recorded within 24 hours.The patients were given insulin therapy by intravenous micro-dosage pump,and the total doses of insulin were recorded.All the patients with 28-day survival were followed up.Results The plasma levels of copeptin of severe pneumonia patients after admission were positively correlated with APACHE Ⅱ (r =0.481,P =0.001) and blood glucose (r =0.417,P =0.004),and were also correlated with the total insulin doses in patients under insulin therapy (blood glucose ≥ 10.0 mmol/L).The plasma levels of copeptin of the survival group were significanatly higher than the non-survival group,COX regression multivariate analysis showed that copeptin was an independent risk factor of death in severe pneumonia patients (P =0.005,OR =1.022,95％ CI:1.00-1.044),and the area under the ROC curve was 0.740 9 (P =0.006).Conclusion The plasma levels in all patients with copeptin of severe pneumonia after admission is positively correlated with APACHE Ⅱ and stress hyperglycemia,suggesting a useful approach to the judgment of disease severity,outcome and glucose control.

OBJECTIVE:To optimize the extraction technology of total volatile oil from Cinnamomum cassia Presl. and Cyper-us rotundus L. in Hegan lipi granules. METHODS:With the extraction rate of total volatile oil as the index,Box-Behnken response surface method was applied to investigate the effects of water amount,soaking time for medicinal materials and extraction time by distillation on the amount of the extracted volatile oil and optimize the extraction technology of total volatile oil from C. cassia Pre-sl. and C. rotundus L. in Hegan lipi granules,and verification tests were conducted. RESULTS:The relevant coefficient of the es-tablished quadratic polynomial regression model of 3 variable factors was 0.970 5. The optimal extraction technology was as follows as 5 times as much as the amount of medicinal materials of water,soaking time of 2.5 h,extraction time of 6.0 h. Verification tests showed the extraction rate of total volatile oil was 1.65%,with a deviation rate lower than 2% compared to the predicted value of 1.67%. CONCLUSIONS:The established model is reliable with good predictability. The optimal technology can be used for the ex-traction of total volatile oil from C. cassia Presl. and C. rotundus L. in Hegan lipi granules.

Objective To study the relationships between androgen receptor (AR)expression and the clinicopathological features and prognosis of young patients (age≤35 years)with early-onset breast cancer in Xinjiang.Methods One hundred and forty-five young patients (age≤35 years)with invasive breast cancer who underwent surgery in Affiliated Cancer Hospital of of Xinjiang Medical University from January 201 0 to December 201 2 were collected.The expressions of AR in 1 45 patiens with early-onset breast cancer were detec-ted by immunohistochemical method.The relationships between AR expression and the clinicopathological fea-tures and prognosis of patients with early-onset breast cancer were retrospective analysed.Results The posi-tive rate of AR expression of patients with early-onset breast cancer in Xinjiang was 69.0% (1 00 /1 45).Single factor analysis showed that AR expression was related with neurovascular invasion (χ2 =5.309,P =0.021 ) and lymph node metastasis (χ2 =6.073,P =0.01 4),but it was not related with ethnicity (χ2 =0.097,P =0.755),age (χ2 =0.045,P =0.831 ),feeding history (χ2 =0.066,P =0.797),family history of cancer (P =0.556),histological grade (P =0.469),tumor size (χ2 =1 .006,P =0.605)and clinical stage (χ2 =4.381 ,P =0.223).The median follow-up time was 47 months.There was no significant difference between AR expression and disease-free survival of patients with early-onset breast cancer (χ2 =1 .972,P =0.1 60). Conclusion Early-onset breast cancer patients with AR positive are more likely to appear lymph node metasta-sis and neurovascular invasion than the patients with AR negative,which has certain guidance means for the treatment of early-onset breast cancer with lymph node metastasis positive.

Objective To explore the possibility of differentiation of bone marrow mesenchymal stem cells(MSCs)into alveolar epithelial cells and bronchial epithelial cells.Methods MSCs were isolated from a male SD rat and were labeled with DAPI before injection.75 female recipient SD rats were divided randomly into five groups:lung injury group,MSCs treatment group,MSCs control group,mononuclear cells control group and normal control group.One,two and four weeks after the engraftment,five rats in each group were sacrificed and their lungs were harvested.Alveolar structure was observed by HE staining,and immunofluorescent examination was carried out to detect the positive staining of pan-cytokeratin.Results Some blue DAPI-labeled cells could be seen in alveolar wall in MSCs treatment group one,two and four weeks after MSCs engraftment,and some of these blue DAPI-labeled cells were found to express pan-cytokeratin.It was also found that some blue DAPI-labeled cells could be found in bronchial wall two weeks after MSCs engraftment and some of them were pan-cytokeratin positive.However,these phenomena were not found in other groups.Conclusion Rat MSCs could differentiate into alveolar epithelial cells and bronchial epithelial cells in injured lung induced by bleomycin exposure.

Objective To investigate the effects of mesenchymal stem cells(MSCs) on the recovery of gentamicin-induced acute renal failure(ARF),and explore its possible mechanism.Methods MSCs were isolated from a male SD rat and labeled with DAPI before injection.ARF rat model was reproduced by hypodermic injection of gentamicin.At the time that gentamicin was injected,MSCs were injected through tail vein.Thirty SD rats were randomly divided into three groups(10 each): MSCs injection group(group M),DMEM-F12 infusion group(group D) and control group(group C).Rats were sacrificed on day 7 after gentamicin injection.The levels of serum Scr and BUN were determined,and the renal tissue was sampled for pathological observation.Cell apoptotic index(AI) was calculated after terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling(TUNEL).The expressions of Bcl-2,Fas and FasL proteins and PCNA were determined by immunohistochemical technique.The distribution of DAPI positive MSCs in kidney of recipient SD rats was examined by immunohistochemistry.Results HSK score(5.5?0.8 vs 10.2?0.8,P

Tuberculous meningitis is an important global medical problem which gives rise to high morbidity and mortality. It is the most severe form of extrapulmonary Mycobacterium tuberculosis. Comprehensive prevention effort, prompt diagnosis and rational treatment are all keys to improving treatment outcomes; yet many unsolved problems remain. On the other hand, the new problems, such as HIV co-infection and drug-resistance are posing important challenges. This review outlines the epidemiology, pathogenesis, diagnosis, management and prognosis of tuberculous meningitis. We mainly focus on research carried out in the recent decades, giving special attention to the work done among the Asian populations
Tuberculosis, Meningeal