BACKGROUND:Drug eluting stents and endothelium stents for clinical treatment of vascular stenosis can lead to delayed endothelialization and restenosis. A rapamycin eluting stent combined with CD34 antibody can play a synergistic role to offset delayed endothelialization and intimal hyperplasia due to antiproliferative drugs, but it is stil in the pilot phase. OBJECTIVE:To observe the ability of rapamycin eluting stent combined with CD34 antibody to capture endothelial progenitor cels, and to observe the differentiation characteristics of the captured cels. METHODS:Scanning electron microscope and indirect immunofluorescence were used to observe the morphology and differentiation characteristics of captured endothelial progenitor cels. Under a fluorescence microscope, we observed the captured endothelial progenitor cels and the degree of endothelialization after implantation of the rapamycin eluting stent combined with CD34 antibody into rabbit ear vein. RESULTS AND CONCLUSION:Under the scanning electron microscope, fusiform-like cels with a diameter of 6-8 μm were captured by the composite stent, and 24 hours later, the cels became ful-shaped. The captured cels had the appearance characteristics of endothelial progenitor cels. Results from indirect immunofluorescence observation showed that there were a lot of red fluorescent spots on the coating which represented adherent cels positive for vascular endothelial growth factor receptor-2; the composite stent was largely covered with vascular endothelial cels at 24 hours after stent implantation, and fuly covered at 48 hours, but there was no abnormal cel cluster. These findings indicate that the rapamycin eluting stent combined with CD34 antibody can be specific to rapidly capture endothelial progenitor cels in the peripheral blood, and the stent can be completely covered with vascular endothelial cels at 48 hours after stent implantation, thereby achieving rapid endothelialization and promoting the repair of endothelial cels.

Objective To investigate the genetic features related to drug-resistance of a strain of pan-drug resistant Acinetobacter baumannii. Methods The susceptibilities to 15 antibacterial agents were detected in Acinetobacter baumannii by K-B paper diffusing method. A strain of pan-drug resistant Acinetobacter baumannii was selected. The drug-resistant genes of the selected strain were amplified by polymerase chain reaction (PCR) , including 15 extended-spectrum β-lactamase genes, 3 metal β-lactamase genes, 2 AmpC enzyme genes, 7 aminoglycoside modifying enzyme genes and the genetic markers of integron I and transposons. Results The strain was resistant to 14 antibacterial agents except cefoperazone sodium/ sulbactam. β-lactamases genes ( TEM, OXA-23 and ADC) , aminoglycosides modification enzyme genes [aac(3)- I , aac(6')- I b and ant(3")- I ] , integron I qacEAi-sull and transposon tnpU were positive in PCR amplification. A new subtype of AmpC (chromosome) desoxyribonucleic acid ( DNA) was detected. Conclusion Acinetobacter baumannii is of high resistance to most antibacterial agents, and the mechanisms of the resistance are complex.

Breast cancer susceptibiIity gene 1( BRCA1)is a tumor suppressor,but mutated get BRCA1 is cIoseIy reIated to the deveIopment of breast cancer. Current study has reveaIed that BRCA1 can get invoIved in the DNA damage repair process as a key mediator. DNA doubIe-stranded break (DSB)is the most serious form in DNA Iesions,and BRCA1 pIays an important roIe in repairing DSB through reguIation of homoIogous recombination( HR). In this articIe,the moIecuIar mechanism of BRCA1 in reguIating HR is reviewed with reference to the activity of functionaI domains in BRCA1 struc-ture,the mutations occurring in main domains of BRCA1,the reIationship of BRCA1 with BRCA2, Rad51 or CtIP,and phosphoryIation of BRCA1. In addition,the association of the defective BRCA1-mediated HR repair mechanism with the sensitivity to different DNA damaging agents and synthetic IethaIity in tumor ceIIs is aIso addressed.

Tuberculous meningitis is an important global medical problem which gives rise to high morbidity and mortality. It is the most severe form of extrapulmonary Mycobacterium tuberculosis. Comprehensive prevention effort, prompt diagnosis and rational treatment are all keys to improving treatment outcomes; yet many unsolved problems remain. On the other hand, the new problems, such as HIV co-infection and drug-resistance are posing important challenges. This review outlines the epidemiology, pathogenesis, diagnosis, management and prognosis of tuberculous meningitis. We mainly focus on research carried out in the recent decades, giving special attention to the work done among the Asian populations
Tuberculosis, Meningeal

Objective To study the changes of plasma copeptin level in pneumonia patients and to explore the correlation between copeptin and disease severity,outcome as well as stress hyperglycemia.Methods A total of 45 patients with severe pneumonia were included in a prospective observation.The plasma levels of copeptin,CRP and blood glucose were measured after admission,and the APACHE Ⅱ scores were recorded within 24 hours.The patients were given insulin therapy by intravenous micro-dosage pump,and the total doses of insulin were recorded.All the patients with 28-day survival were followed up.Results The plasma levels of copeptin of severe pneumonia patients after admission were positively correlated with APACHE Ⅱ (r =0.481,P =0.001) and blood glucose (r =0.417,P =0.004),and were also correlated with the total insulin doses in patients under insulin therapy (blood glucose ≥ 10.0 mmol/L).The plasma levels of copeptin of the survival group were significanatly higher than the non-survival group,COX regression multivariate analysis showed that copeptin was an independent risk factor of death in severe pneumonia patients (P =0.005,OR =1.022,95％ CI:1.00-1.044),and the area under the ROC curve was 0.740 9 (P =0.006).Conclusion The plasma levels in all patients with copeptin of severe pneumonia after admission is positively correlated with APACHE Ⅱ and stress hyperglycemia,suggesting a useful approach to the judgment of disease severity,outcome and glucose control.

Objective To study the relationships between androgen receptor (AR)expression and the clinicopathological features and prognosis of young patients (age≤35 years)with early-onset breast cancer in Xinjiang.Methods One hundred and forty-five young patients (age≤35 years)with invasive breast cancer who underwent surgery in Affiliated Cancer Hospital of of Xinjiang Medical University from January 201 0 to December 201 2 were collected.The expressions of AR in 1 45 patiens with early-onset breast cancer were detec-ted by immunohistochemical method.The relationships between AR expression and the clinicopathological fea-tures and prognosis of patients with early-onset breast cancer were retrospective analysed.Results The posi-tive rate of AR expression of patients with early-onset breast cancer in Xinjiang was 69.0% (1 00 /1 45).Single factor analysis showed that AR expression was related with neurovascular invasion (χ2 =5.309,P =0.021 ) and lymph node metastasis (χ2 =6.073,P =0.01 4),but it was not related with ethnicity (χ2 =0.097,P =0.755),age (χ2 =0.045,P =0.831 ),feeding history (χ2 =0.066,P =0.797),family history of cancer (P =0.556),histological grade (P =0.469),tumor size (χ2 =1 .006,P =0.605)and clinical stage (χ2 =4.381 ,P =0.223).The median follow-up time was 47 months.There was no significant difference between AR expression and disease-free survival of patients with early-onset breast cancer (χ2 =1 .972,P =0.1 60). Conclusion Early-onset breast cancer patients with AR positive are more likely to appear lymph node metasta-sis and neurovascular invasion than the patients with AR negative,which has certain guidance means for the treatment of early-onset breast cancer with lymph node metastasis positive.

OBJECTIVE:To optimize the extraction technology of total volatile oil from Cinnamomum cassia Presl. and Cyper-us rotundus L. in Hegan lipi granules. METHODS:With the extraction rate of total volatile oil as the index,Box-Behnken response surface method was applied to investigate the effects of water amount,soaking time for medicinal materials and extraction time by distillation on the amount of the extracted volatile oil and optimize the extraction technology of total volatile oil from C. cassia Pre-sl. and C. rotundus L. in Hegan lipi granules,and verification tests were conducted. RESULTS:The relevant coefficient of the es-tablished quadratic polynomial regression model of 3 variable factors was 0.970 5. The optimal extraction technology was as follows as 5 times as much as the amount of medicinal materials of water,soaking time of 2.5 h,extraction time of 6.0 h. Verification tests showed the extraction rate of total volatile oil was 1.65%,with a deviation rate lower than 2% compared to the predicted value of 1.67%. CONCLUSIONS:The established model is reliable with good predictability. The optimal technology can be used for the ex-traction of total volatile oil from C. cassia Presl. and C. rotundus L. in Hegan lipi granules.

Objective To explore the possibility of differentiation of bone marrow mesenchymal stem cells(MSCs)into alveolar epithelial cells and bronchial epithelial cells.Methods MSCs were isolated from a male SD rat and were labeled with DAPI before injection.75 female recipient SD rats were divided randomly into five groups:lung injury group,MSCs treatment group,MSCs control group,mononuclear cells control group and normal control group.One,two and four weeks after the engraftment,five rats in each group were sacrificed and their lungs were harvested.Alveolar structure was observed by HE staining,and immunofluorescent examination was carried out to detect the positive staining of pan-cytokeratin.Results Some blue DAPI-labeled cells could be seen in alveolar wall in MSCs treatment group one,two and four weeks after MSCs engraftment,and some of these blue DAPI-labeled cells were found to express pan-cytokeratin.It was also found that some blue DAPI-labeled cells could be found in bronchial wall two weeks after MSCs engraftment and some of them were pan-cytokeratin positive.However,these phenomena were not found in other groups.Conclusion Rat MSCs could differentiate into alveolar epithelial cells and bronchial epithelial cells in injured lung induced by bleomycin exposure.

Objective ABCG2(ATP-binding cassette superfamily G member 2)is a member of ABC transporter superfamily,and participates in the mechanisms of high resistance to chemotherapeutic agents in tumor treatment.The aim of present study is to investigate the expression and significance of ABCG2 in lung cancer tissues.Methods Lung cancer tissues were obtained from 83 patients(64 NSCLC,19 SCLC)by bio-autopsy or surgery.No patients had received chemotherapy or radiotherapy before bio-autopsy or surgery.83 lung cancer specimens were analyzed for ABCG2 protein expression by using immunohistochemistry.Negative controls had the primary antibody eliminated.Human placenta tissues were used as positive control.In addition,five specimens of normal lung tissues were included for comparative study.Results The present study confirmed the predominant localization of ABCG2 transporter in plasma membrane.Some of ABCG2-positive tumors showed mixed membranous and cytoplasmic staining.ABCG2 expression was found in 71.9%(46/64)of NSCLC and 10.5%(2/19)of SCLC.The level of ABCG2 expression was significantly higher in NSCLC than in SCLC(P

Objective: To study the relationship between different patterns of invasion and extracapsular spread in lymph node metastasis from squamous cell carcinoma of tongue. Methods: Pattern of invasion described by Anneroth was used to evaluate the malignance grade of tongue cancer and immunohistochemical and HE staining were used to detect the extracapsular spread in successive cases of tongue cancer with radical neck dissection. Results: Lymph node metastasis was observed in 0/5 of the cases with pattern I invasion,3/8 pattern II,5/6 pattern III and 1/1 pattern Ⅳ. Extracapsular spread occurred as totally or partly replacement of the lymph node by the tumourcells. The tumour cells infiltrated between lymph nodes, perinodal fibroadipose tissue or sternocleidmastoid muscle in the form of cell cluster or isolated cell. Conclusion: Pattern of invasion is a significant factor for evaluating the malignance grading of tongue cancer. Radical neck dissection should be used to treat the tongue cancer with pattern III or IV invasion.