Objective To explore the rising degree of hemodiastase,lipase and the relationship with acute pancreatitis with diabetic ketoacidosis(DKA).Methods 36 diabetic ketoacidosis patients with increased trypsin were divided into non pancreatitis group(20 cases)and pancreatitis group(16 cases)based on the results of abdominal CT.And the blood amylase,urine amylase,blood fat enzyme levels of the two groups were measured and compard.Results The blood amylase of the pancreatitis group was(275.0±10.5)U/L,and that of pancreatitis group was(615.4±17.8)U/L,the difference between two groups was obvious(P＜0.01);The blood lipase of pancreatitis group was(2125.0±50.4)U/L,and that of pancreatitis group was(2021.0±19.8)U/L,there was no significant difference between the two groups(P＞0.05).The blood amylase and abdominal CT results compliance high.Conclusion Pure DKA can cause diabetes pancreatic enzyme increases,and in the diagnosis of acute pancreatitis,the specificity of blood amylase is high.

Cardiovascular Diseases ; epidemiology ; Humans ; Risk Factors

Objective To study the significance of plasma homocysteine(Hcy),high sensitivity C-reactive protein(hs-CRP)and cardiac treponin T(cTnT)changes in the patients with coronary heart diseases(CHD).Methods From August 2012 to November 2013,240 patients with CHD in our hospital were collected as the CHD group and 250 individuals of healthy physical examination served as the control group.Serum Hcy,hs-CRP and cTnT were detected by using the enzymatic cycle assay,immune scatter turbi-dimetry and electrochemiluminescence assay respectively.The data were statistically analyzed by the SPSS software.Results The serum levels of Hcy,hs-CRP and cTnT in the CHD group were (24.89±17.78)μmol/L,(17.43±35.89)mg/L and (0.76±2.36) ng/mL respectively,which were significantly higher than those in the control group(P <0.001).Moreover,the positive rate of Hcy in the CHD group was the highest (81 .25%),followed by hs-CRP(64.58%)and cTnT (58.75%).Conclusion Hcy,hs-CRP and cTnT played important roles in the development and progression of CHD,so reducing Hcy level may be an important pathway for preventing and treating CHD.

The polymorphisms of the TPOX locus in two population were studied by PCR technique followed by the denatured PAGE and silver staining.Seven alleles were detected in each population of 100 unrelated Northern Chinese Han individuals and 93 unrelated African(Xhosa)individuals in South Africa respectively.No deviation from the Hardy Weinberg equilibrium was demonstrated.The power of discrimination(DP)and the exclusion probability(EPP)were 0 7978 and 0 4625;0 9141 and 0 6125 respectively.Comparison of gene frequency distribution showed that there is significant difference between different races.In addition,three rare genes were detected in we Africans(Xhosa)individuals.Sequencing proved that the only difference among them was the number of repeat unit in the region amplified by the specific primer pair.It might be due to the unequal exchange between chromosomes which was resulting in the production of a chimera.Our study showed that the TPOX locus has a high discriminating power which is important for the study of forensic medicine and human genetics\;

Objective To study the genetic polymorphisms of DYF155S1 locus in 107 unrelated individuals both in the Han population, China and the Japanese population. Methods MVR-PCR, automated fluorescence detection and DNA sequence analysis were carried out for studying. Results Five types of repeat unit and one type newly named type 6 which was resulted from the T22A substitution on Type 1 were detected. Type 6 is monopolized by Japanese and may be regarded as a racial characteristic genetic marker. The common arrangement of the repeat units was 3134 which were detected at 73.44 % and 67.44 % in the Han and Japanese populations respectively. The abundance of arrangement of 3134 is the character of yellow race. Next to the arrangement of 3134 was the arrangement of 134 which was detected at 17.19% in the Han population while the arrangement of 6134 was detected at 16.28% in the Japanese population. The average number of type 4 unit at the 3'terminal was 8.8 in the Japanese population much lower than 12.5 in the Han population. Conclusion This study implies that DYF155S1 locus is an important geneticmarker with good genetic polymorphisms. The population difference between the Han and the Janpanes is significant.

Objective To investigate the molecular structure, gene expression and detecting methods of some new variant alleles at FUT2 locus. Method We examined four new variant alleles at FUT2 locus using PCR, RFLPs, gene recombination, DNA sequencing and techniques related to gene expression. Results Three missense gene mutations which were C664T, G868A and G760A respectively were found in three New Guinea individuals. Absence of the glycosyltransferase activity in all three enzymes coded by above three missense gene mutations were confirmed by gene expression techniques. Nonsense mutation A660T was found in one Chinese Han individuals. Changing of sequence of endonuclease SacI resulted from C664T and A660T can be detected by RFLP method. Weak peaks of variats might be missed if DNA sequencing was used to detect heterozygotes. RFLP method can't be used to determine specific site of variation within identified sequence of endonuclease. Conclusion All three FUT2 genes resulted from three mutations C664T, G868A, and G760A were non-secretor genes. More than two methods must be used for checking results each other when detect DNA sequence polymorphisms.

Objective To study the genetic polymorphism and population diversity of three SNP loci and their haplotypes on human Y Chromosome. Methods Genetic polymorphisms, population diversity and hap-lotypes of these SNP loci, M4, M9 and M122, on human Y chromosome, in 140 wnrelated individuals from Zang Nationality in China, Japanese, Negroes and white men from southern Africa were studied by using PCR-RFLPs and DNA sequencing analysis. Results No polymorphism was found on M4 locus, each sample was M4A. All samples were wild type. 3 haplotypes were found. In all Negroes there was a wild haplotype of M4A/M9C/M122T. A haplotype of M4A/M 9G/M122T were found in 8 white men. No M122C gene was found. The haplotypes of M4A/M9C/M122T were mainly found in both Japanese and Chinese, The haplotype frequencies in Japanese and Zang population in china were 0.5 and 0.65 respectively. The probability of discrimination power (DP) and the excluding probability of paternity (EPP) were 0.6191 and 0.4994 respectively and the haplotype of M4A/M9C/M122C was absent. There was a remarkable difference (P

48 hours.The expressions of leukocyte common antigen(LCA) and intercellular adhesion molecule-1(ICAM-1) in the brain tissues collected during the surgery were detected by immunohistochemistry staining method.Results LCA and ICAM-1 immunopositive cells and microvessels were verified in all 29 cases of ICH groups,and the expressions were more than control group(all P48 hours group were higher than that in 6～24 h group(both P48 hours group.Conclusions Acute inflammatory reaction occurs in acute period of ICH.Expression of ICAM-1 reinforces gradually during early 48 hours since the onset of ICH.

Objective To explore the outcome and diagnostic methods of umbilical cord winds of the perinatal. Methods Retrospective analysis of 2350 cases of umbilical cord winds in our hospital was conducted. We selected 2400 cases as control. We calculated both the incidence rate of cesarean section, fetal distress and neonatal hypoxic - ischemic encephalopathy. Results Umbilical cord winds accounted for 20.1 percent of the total number of deliveries in the same period. The incidence rate of fetal distress and cesarean section was significantly high. Bsound ultra was 98.9% diagnosis. The electronic fetal care was 99.7% in diagnosis. Color Doppler ultrasound was 100% in diagnosis. Conclusion Umbilical cord winds is a common complication of pregnancy, and decision is also an important factor for delivery mode. Umbilical cord winds will increase obviously in the incidence of fetal distress and perinatal morbidity. The main method for prenatal diagnosis is the color Doppler ultrasound examination, such as B - and E - fetal care. Color Doppler ultrasound technique is the best way for non - invasive is observation of intrauterine blood circulation changes. In helpful for the judge of the progress and outcome of pregnancy, fetal growth and development of the situation.

Objective To evaluate the precision of the ACCU-CHEK Performa glucose monitor,the Optium Xceed glucose monitor,the Breeze glucose monitor and the Contour glucose monitor.Methods A total of 102 patients (diabetic patients or un-diabetic patients) who visited our endocrinology outpatient department were randomly selected.The venous blood samples were collected using the laboratory auto-analyzer,and the synchronous finger tip capillary blood were collected using the ACCU-CHEK Performa glucose monitor and the Optium Xceed glucose monitor,or using the Breeze glucose monitor and the Contour glucose monitor.Results The correlation coefficients between VPG by the laboratory auto-analyzer and CBG by the four kinds of glucose monitors was good,and R values were 0.990,0.985,0.963,0.952,and there was no significant difference between the CBG of Breeze glucose monitor and the same VPG(P ＞ 0.05).When the blood glucose concentration was higher than 4.2mmol/L,and compared with the VPG detected by synchronous measurement laboratory,95％ of the CBG test results detected by four kinds of blood glucose meters were in the range of ± 20％.Conclusion The four kinds of glucose monitors can provide high accurate.