Objective To explore the relationship between P-selection,nitric oxide(NO),high-sensitive-C-reactive protein(hs-CRP)and type 2 diabetes mellitus(T2DM)with macroangiopathy.Methods In sernm of 125 T2DM subjects(60 subjects with macroangiopathy and 65 subjects without macroangiopathy)and 55 normal controls,the activity of P-selection,NO and hs-CRP was detected by ELISA,Nitrate Reductase Assay and scattering immunoturbidimetric method,respectivley.Results The activity of serum P-selection and hs-CRP was significantly higher in diabetic macroangiopathie group than in non macroangiopathic group and control normal.Serum NO was significantly lower in diabetic rnacroanglopathic group than in non macroangiopathic group and control normal.Serum P-selection was positively correlated with hs-CRP-systolic blood pressure(SBP),LDL-C,and was negatively correla- ted with NO:P-selection,hs-CRP,LDL-C and SBP were independently risk factors for T2DM with macroangiopathy. Conclusion Determination of P-selection and NO is very important for prevention and diagnosis of diabetic mac- roangiopathy in early stage.

The evaluation of biomaterial biocompatibility is a key step before the clinical application.With the rapid development of molecular biology,scientists have begun to evaluate biomaterial biocompatibility at molecular level and proposed the concept of molecular biocompatibility.Researchers'main tasks at present are to identify more molecular markers using molecular biology technology and to establish standards for evaluating molecular biocompatibility of biomaterial,which will provide the guidance for the design of better biomaterials.

Objective The purpose of this study is to analyse the expression of tumor necrosis factor (TNF)-related apoptosis inducing ligand(TRAIL)and its receptors in muscle tissue from patients with idiopathic inflammatory myopathies(ⅡM)and investigate the possible role of TRAIL system in the Dathogenesis of IIM.Methods TRAIL and its receptors DR4,DR5,DCRl and DCR2 were detected in the musck biopsy tissue from 36 patients with ⅡM(13 polymyositis,23 dermatomyositis)and 9 heMthy controls bv using immunohistochemistry. Results The expression of TRAIL and its receptors was f10und in muscle tissue samples from the IIM patients and healthy controls.The expression of TRAIL,DR4 and DCR2 in the muscle tissue from the IIM patients was significantly higher than those from healthy controls(all P values< 0.05).The expression of TRAIL,DCRl and DR4 was also detected in the infiltrating lymphocytes in endomysium and in the interstitial tissue around blood vessels.Conclusion The diversity in expression of TRAIL and its receptors between patients with IIM and healthy controls suggests the hypothesis of a crucial role of TRAIL in the pathogenesis and the pathology of IIM.

β-catenin is an important signaling transduetion and adhesion molecules.Mutation of the betacatenin gene,CTNNB1,is a common case in pediatric tumors,which may induce development and metastasis.In hepatoblastoma,48%have CTNNB1 mutation.In Wilms tumor,mutation only occurs in cases with WT1 gene mutation.In neuroblastoma,mutation is also found.

Objective To observe the biomechanics of the anterior and posterior interosseous talo-calcaneal ligaments (ITCLs) . Methods Load-displacement characteristics of the subtalar joint were studied in 12 cadaver specimens whose ankle joints were mutilated. The ankle articular surfaces of the talus and the post-calcaneus were exposed. Bone blocks were then embedded in polymethylmethacrylate. The an-terior ITCLs were abscised in 6 of them, and the posterior ITCLs in the other 6. A multi-functional biome-chanical machine was used to perform the biomechanical tests on the specimens. Results When the anterior ITCLs were cut, the tali moved to the anterior-lateral side. When the posterior ITCLs were cut, the tali mostly moved to the anterior-interior side. Conclusions The anterior and posterior ITCLs have dif-ferent roles in maintaining stability of the subtalar joint. Since the posterior ITCLs seem more important than the anterior ones, they should have priority in ITCL reconstruction.

OBJECTIVE:To explore the effect of rehabilitation training on restenosis following stent implantation in patients with coronary atherosclerotic heart disease METHODS:A computer.based online search of VIP database was performed to search articles regarding restenosis following stent implantation and rehabilitation treatment for patients with coronary atherosclerotic heart disease,published between January 2000 and October 2009,with key words"coronary artery,stent implantation,intracoronary restenosis,rehabilitation exercise".The data were collected,and the references of each article were reviewed A total of 20 articles were included.RESULTS:Intracoronary stent has become an effective treatment for coronary atherosclerotic heart disease,and intracoronary restenosis has been focused in clinical studies.Prophase treatment following stent implantation is very important for coronary atherosclerotic heart disease.Rehabilitation exercise in combination with antiplatelet anticoagulatiOn and lipid treatment can reconstruct coronary arterial blood transport and significantly reduce incidence of restenosis and thrombus.CONCLUSION:Regular rehabilitation exercise following stent implantation can ameliorate degree of vascular restenosis.

BACKGROUND:Periosteal osteoblasts possess strong reproductive activity,as well as osteoblastic differentiation potential,which is an ideal seed cell if can shorten the culture time.OBJECTIVE:Modified enzymatic digestion was used to culture rabbits'osteoblasts.and to study the adherence and proliferation of osteoblasts on the sudace of sandblasting titanium.MEITHODS:Periostea were harvested from the theanteromedial surface of the proximal tibia of male,Japanese white rabbits,and cultured as follow:①Routine method:Digested with 0.25%trypsinase at 37 ℃ for 30 minutes,followed by digestion with 0.1%type I collagenase at 37 ℃ for 30 minutes,vibration.removed trypsinase and dried.After 2 hours,DMEM containing 15% fetal bovine serums were added.②Modified method:30 minutes culture of type I collagenase was prolonged to 1 hour.The osteoblasts were identified by alkaline phosphatase staining and calcium node staining.The adherence and proliferation of osteoblasts cultured on sandblasting surface were measured by scanning electron microscopy and MTT.RESULTS AND CONCLUSION:Five days after culture.the periosteal steoblasts crawled out from tissues,gathered as monolayer with tdangle or polygon at after 25 days of modified culture.After 1 month of culture,superposition growth of calcium nodus appeared.The cultured cells possessed the morphological characteristic and biological behavior of osteoblasts.which were positive to alkaline phosphorase and calcium node staining.The time of cells cultured with routine method covered flask delayed 12 days than modified method.The osteoblasts were inseted into sandblasting titatium with pseudopodium.However,the adherence and proliferation of osteoblasts cultured on sandblasting surface had no obviously difference between two culture methods.The results suggested that modified enzymatic digestion can sho～en the culture time without effect on adherence and proliferation of osteoblasts.

The development of the international spread of Chinese medicine facing the serious challenge of the global competition with the tentacles of Chinese medica;communication in the world are spreading even wider.How to make countermeasures.seize an opportunity and receive the challenge to improve international competitiveness effectively and promote sound and rapid development of the internationalization of Chinese medicine have become the focus of common attention.In this paper,I'll discuss some problems in the development of the international communication of the culture of Chinese medicine such as the main task and the principles.

Objective To detect the fluctuations of homoeysteine(Hcy),endothelin(ET),mononitrogen monoxide(NO),folic acid(FA) and vitamine Bi2 (VitB_(12)) in the plasma of patients suffered from acute cerebral infarction and to investigate the clinical significance.Methods 140 blood specimens from cerebral infarction patients and 61 specimens from healthy individuals were obtained in this study and a series of items of Hey,ET,NO,FA and VitB_(12) were detected.Results The Hey [(17.15±4.63) μmol/L]and ET [(32.62±18.76) μmol/L]were increased in acute cerebral infarction(ACI) group compared with that in control group[(10.12±2.62) μmol/L,(20.08±13.38) μmol/L respectively]and the difference was significant (P＜0.01).The levels of NO [(39.82±7.26) μmol/L],FA [(7.54±4.01) ng/ml]and VitB_(12) [(247.70±119.85) pg/ml]were decreased compared with control group [(60.22±6.28) μmol/L,(10.08±3.86) ng/ml,(411.52±194.58) pg/ml respectively]and the differences significant (P＜0.01).Gender and hypertension affected the values of Hey.Conclusions Hcy and ET were increased while NO,FA and VitB_(12) were decreased in acute cerebral infarction.Hcy had positive correlation with ET and negative correlation with FA and VitB_(12).

Vascular endothelial growth factor(VEGF)is a major angiogenic factor that regulates multiple endothelial cell functions. After cerebral ischemia, VEGF not only promotes vascular endothelial proliferation and migration. involving in angiogenesis and increasing vascular permeability, but also plays important roles in both neuroprotection and neurogenesis. This article reviews the roles of VEGF in ischemic cerebral injury.