2.Optimization of Cultivation Conditions for Extracellular Polysaccharide and Mycelium Biomass by Pholiota Squarrosa
Yun-Xiang WANG ; Zhao-Xin LU ;
Microbiology 1992;0(06):-
Response surface methodology (RSM) based on a three-level three-factor Box-Behnken design of experiments was used to optimize the extracellular polysaccharide content and the mycelium biomass by submerged cultivation using Pholiota squarrosa AS 5.245. The critical factors selected for the investigation were temperature, time of cultivation and volume of medium, based on the results of our previous Plackett-Burman design. The objectives of this present work were to locate optimum levels of these process parameters, and to find out interactions among them for enhancement of the yield of extracellular polysaccharide and mycelium biomass. By solving the regression equation and also by analyzing the response surface contour plots, the optimal process conditions were determined: under conditions of temperature, 28.07℃; cultivation time, 8.79 d and volume of medium, 68.51 mL, the prediction of extracellular polysaccharide content (EPC) by Pholiota squarrosa AS 5.245 was 1062.69 ?g per milliliter of fermentation liquor. While cultivation temperature, time and volume of medium were 27.60℃, 9.42 d and 54.20 mL respectively, the mycelium biomass expressed as dry cell weight (DCW) was 11.32 mg?mL -1. In order to simultaneously obtain the maximum yield of EPC and DCW, the above conditions would be located at 27.62℃, 9.19 d and 64.10 mL. In these conditions, the maximum predicted yield of EPC and DCW were found to be 1050.64 ?g?mL -1 and 11.10 mg?mL -1, respectively. These predicted values were also verified by validation experiment.
3.Immune hyporesponsiveness induced by immature dendritic cells prolonged rat kidney allograft survival
Xiang WANG ; Hong ZHAO ; Yuanfang ZHANG
Chinese Journal of Organ Transplantation 1996;0(04):-
Objective To study the effect of immature dendritic cells (imDC) transfusion in combination with bone marrow transplantation (BMT) on rat kidney allograft survival and its possible mechanism. Methods Renal allograft from DA rat was transplanted to Lewis rat. Forty recipient rats were randomized into 5 groups: (1) Negative control group; (2) imDC group: Lewis rats accepted imDC transfusion in the amount of 2? 107 only; (3) BMT group: Lewis rats accepted bone marrow transplantation in the amount of 2?108 only; (4) imDC + BMT group: Lewis rats accepted both imDC and BMC; (5)Third party donor group: Lewis rats accepted renal allograft from Wistar rats. One-way MLR was performed to assay splenic cell proliferation to allogeneic T cells. Exogenous IL-2 was added at the outset of another group as the former one-way MLR. Normal Lewis rat accepted splenic cells from tolerant rat in the amount of 1?108. DTH was assayed in the trans-tolerance model. Cells from spleen and thymus were harvested from recipient rats for detecting chimerism by flow cytometry. Results The median survival time (MST) of the renal allografts was (7. 12 ? 1. 25) days in negative control group, (24. 36 ? 3. 20)days in imDCs group and (7. 87 ? 2. 10)days in BMT group, respectively. In combined group, the MST was prolonged to (80. 75 ? 16. 88)days, which had significant difference as compared with the former three groups (P
4.Evaluation of the clinical effect in the treatment of 42 patients with acute cerebral infarctions with Qiangli Tianma Duzhong Capsule
Xiang SHEN ; Guodong WANG ; Yongbo ZHAO
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To evaluate the clinical curative effect of Qiangli Tianma Duzhong Capsule in the treatment of acute cerebral infarctions. METHODS: 72 patients were randomly divided into two groups:the treatment group and contrast group.The treatment group on the basis of the regular treatment was assigned to 28 days of treatment with Qiangli Tianma Duzhong Capsule.The contrast group on the same regular treatment was assigned to 28 days of treatment with Fufang Danshen Injection.The European Stroke Score(ESS) and clinical curative effect were evaluated in the treatment group and control group on the 28~(th) day. RESULTS: After 28 days,the European Stroke Score(ESS) of the treatment group(6.02?4.81) decreased significantly in the control group(7.88?4.85)(P
5.Effects of different excipients and its compound on extract granulating made by spray drying
Xiuliang WANG ; Daxong XIANG ; Xuyuan ZHAO ;
Chinese Traditional Patent Medicine 1992;0(02):-
Objective: To investigate effects of different excipients and its compounds on extract granulating made by spray drying and to find the best compound of making granule. Methods: Taking difficulty of granulating; obtaining rate, granule dissolution rate, hygroscopicity as assessing index, evaluating effects of different excipients and its compounds on granulating and finding the best compound. Results: Different excipients and its compounds could significantly decrease the hygroscopicity of extract. Different compounds could sharply affect difficulty of granulating, obtaining rate and granule disdoultion while lightly affecting the hygroscopicity. Conclusion: When critical relative moisture is controlled under 65%, the following two compounds can be selected: (1) extract sucrose silion dioxide (500∶115∶25). (2) extract sucrose microcrystalline cellulosec silion dioxide.
6.Recent Research Situation of Heart Rate Variability-based Analysis of the Regulating Effect of Acupuncture on Autonomic Nervous Function
Zhifeng CHEN ; Yunyun ZHAO ; Xing WANG ; Yunxia XIANG ; Ling ZHAO
Shanghai Journal of Acupuncture and Moxibustion 2016;35(6):754-757
Traditional Chinese medicine considers that the final purpose of acupuncture treatment for disease is to harmonize yin and yang. Acupuncture redresses a balance between yin and yang by transmitting information to acupoints and then to corresponding visceral tissues and organs and the brain through the meridian system or the nervous system. In recent years, scholars at home thoroughly studied heart rate variability (HRV) analysis for acupuncture treatment of diseases and found that the regulating effect of acupuncture on visceral organs was closely related to autonomic nerves and acupuncture could restore a balance between sympathetic and parasympathetic nerves. Many scholars explored the effect of acupuncture on diseases related to autonomic nervous dysfunction by HRV frequency domain or time domain analysis and gained a certain achievements. This article summarizes recent years’ domestic studies on HRV-based analysis of acupuncture for diseases with autonomic nervous dysfunction and reviews the close relationship between autonomic nerves and diseases and the related mechanism of acupuncture action.
7.Effect of combined thrombolysis and anticoagulation therapy on intimal changes of vein wall after acute thrombosis in rats
Bing YANG ; Qinghua ZHAO ; Yu ZHAO ; Xuehu WANG ; Dongzhou XIANG
Chinese Journal of General Surgery 1997;0(06):-
Objective To observe the intimal changes of inferior caval vein(ICV) wall after combined thrombolysis and anticoagulation therapy for acute thrombosis in rats.Methods The inferior caval venous thrombosis model was established in 105 rats and they were randomly diride into 3 treated groups:heparin(A) treatment group(n=35),urokinase(B) treatment group(n=35),and combination of urokinase and heparin(C) treatment group(n=35),and also eslablished a sham(D) group(n=30).The thrombosed caval veins were taken 1,4,7,14,and 28 d after thrombosis.Changes of thrombus structure and orgainization and intimal hyperplasia were observed by light microscope.The expression areas of collagen fiber were measured by histochemistry.Scanning electron microscopy was used to observe the damage of endothelial cells.Results The intimal hyperplasia in A group was the most severe.The expression area of collagen fiber in group C was less than that of A and B groups(P
9.Evaluation and influential factors of early life quality of patients with severe blunt chest trauma
Xiaowen WANG ; Zhizhen WANG ; Dingyuan DU ; Xiaoyong XIANG ; Xingji ZHAO
Chinese Journal of Trauma 2014;30(2):132-135
Objective To assay the early quality of life and posttraumatic stress disorder (PTSD) and relating influential factors in patients with severe blunt chest trauma (sBCT).Methods Demographic and clinical data of sBCT patients treated between January 2011 and December 2011 were collected.Early quality of life and PTSD symptom level at posttraumatic months 1,3,and 6 were analyzed by using short form 36 health survey (SF-36) and impact of event scale-revised (IES-R) respectively.Furthermore,logistic regression analysis was performed to identify the risk factors associated with quality of life of the patients.Results A total of 107 patients were included in the study.Ultimately,83 patients were available to the 6-month follow-up.A low score for SF-36 remained at posttraumatic 6 months and one-third of the 83 patients sustained mild or severe PTSD symptoms.Major influential factors to posttraumatic quality of life included age,ISS ≥ 20,combined craniocerebral injury,combined spinal and pelvic injuries,posttraumatic complications,and PTSD.Conclusions Early quality of life in sBCT patients is poor.Therefore,the early intervention with identification of specific risk factors is contributive to better quality of life.