1.Observation on the Expression of HBV-DNA in the Umbilical Cord Tissuesof the Died Fetus Delivered From Puerpera with Hepatitis Virus B
Journal of Chinese Physician 2001;0(10):-
Objective To observe the expression of HBV-DNA and whether there were copy of HBV in the umbilical cord tissues of the died fetus delivered from puerpera with hepatitis virus B. Methods 40 cases such died fetus were collected by routine autopsy to obtain umbilical cord tissues.And using in situ molecular hybridization technique detected HBV-DNA. Results For the umbilical cord tissues, there were 40%(16/40) cases detected out HBV-DNA.HBV-DNA mainly localization in the surface of the umbilical cord vessel and in the cytoplasma of the cord vessel's endothelial cells. They were not in the cord vessel's endothelial cells nuclei. Conclusions There were HBV replication in the umbilical cord tissues of the died fetus. But the expression of HBV-DNA in the umbilical cord tissues of the died fetus is not related with the HBV replication status in the pregnant woman veins.
2.Expression of HBV-DNA in the Umbilical Cord Tissues of The Dead Fetus Delivered From Puerperant With Hepatitis Virus B Infection
Danwie WANG ; Wei ZHAO ; Wei LIU
Journal of Chinese Physician 2000;0(12):-
0 05). HBV-DNA were mainly localized on the surface of the umbilical cord vessel and in the plasma of the cord vessel's endothelial cells. HBV-DNA in the cord vessel's endothelial cells nuclei was not seen.Conclusion There were HBV replication in the umbilical cord tissues of the died fetus. But expression of HBV-DNA in the umbilical cord tissues of the died fetus was not related with the HBV replication status in the pregnant woman veins.
3.Repair of rabbit facial nerve with collagen conduits and expanded polytetrafluoroethylene
Wei ZHANG ; Wei LIU ; Jianhua ZHAO
Chinese Journal of Rehabilitation Theory and Practice 2005;11(8):621-622
ObjectiveTo evaluate the effects of expanded polytetrafluoroethylene (e-PTFE) and collagen tubes in the repair of facial nerve in rabbits.MethodsThe facial nerves of rabbits were transected and removed for 5 mm. The nerve ends were then repaired with an e-PTFE or collagen tube, and with an autologous nerve graft as control. After 7 days, 1 month and 3 months, the animals' neural conductive velocity were determined. And then, the nerve specimens were taken out, and the myelinated fibers across the specimen were counted with histological examination.ResultsOn the 7th day, no neural regeneration was observed. But new forming neural fibers across the biocompatible materials and autologous nerve grafts were seen in the following 3 months, while the number and conductive velocity of myelinated fibers varied significantly at the different time points.Conclusione-PTFE and collagen conduits are effective in the repair of peripheral nerves.
4.A meta-analysis about the relation of polymorphism at the sites + 49 of cytotoxic T-lymphocyte associated antigen 4 gene with the susceptibility of Graves' disease in Asian population
Yuqin ZHAO ; Xiaoqing LIU ; Wei LIU
Chinese Journal of Endocrinology and Metabolism 2013;29(11):949-953
Objective To review the quantitative relationship of polymorphism at the sites +49 of cytotoxic T-lymphocyte associated antigen 4 gene with the susceptibility of Graves' disease(GD) in Asian population.Methods The Medline,PubMed,Embase,Web of Science,and Chinese Biomedical Literature Database on disc (CBM disc) databases were searched and used in meta-analysis to analyze the data in the articles collected.Results This analysis included a total of 24 papers,and GD patients and healthy population control group were composed of 4 832 cases and 5 643 subjects respectively.This paper revealed that in the general population,the Chinese people and the Japanese crowd,all the genetic models (allele,co-dominant model,dominant model,recessive model) were statistically significant,indicating that CTLA-4 +49 A allele could reduce the risk of GD.Conclusions The CTLA-4 +49 G→A mutation allele may serve as a factor in reducing the risk of GD susceptibility in Asian population.
5.Protective effects of Huangqi against ischemia /reperfusion injury in rats
Wei WANG ; Qining LIU ; Xinjing ZHAO
Journal of Xi'an Jiaotong University(Medical Sciences) 2009;30(6):712-715
Objective To study the effects and mechanism of Huangqi against myocardium injury induced by ischemia and reperfusion in rats. Methods Totally 30 male Sprague-Dauley rats were randomly divided into three groups: control group (C group), ischemia/reperfusion group (I/R group), ischemic pretreatment with Huangqi group (H+I/R group), with 10 rats in each. The left anterior descending (LAD) coronary artery was ligated to establish the ischemia/reperfusion heart model. Huangqi was administered before the model was established in the H+I/R group. The pathologic changes of myocardial tissues (under light and electron microscopy), content of creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in serum, as well as activities of Na~+K~+-ATPase and Ca~(2+)-ATPase in myocardial tissues were observed. Results Light and electron microscopic examination showed that the necrotic degeneration and pathologic changes of myocardiocytes in Huangqi group were significantly milder than those in the model group. The levels of CK, LDH, MDA were decreased significantly in Huangqi group (P<0.05), the activities of SOD, Na~+K~+-ATPase and Ca~(2+)-ATPase in myocardial tissues in Huangqi group significantly increased (P<0.05). Conclusion Huangqi shows a significantly protective effect on myocardial I/R injury in rats. The mechanism may be related to multiple factors, including improving microcirculation, forming anti-oxygen free radicals and decreasing calcium overload.
6.Value of three-dimensional endometrial volume and 3-dimensional power Doppler imaging in predicting endometrial polyps and endometrial hyperplasia
Wei ZHAO ; Zhi LIU ; Cai CHANG
Chinese Journal of Ultrasonography 2010;19(1):40-42
Objective To evaluate the value of endometrial volume measurement and 3-dimensional power Doppler imaging(3D-PDI) in the differentiation between endometrial polyps and endometrial hyperplasia.Methods One hundred and fifty-six women who diagnosed as thick and inhomogeneous endometrium or hyperechogenic focal intrauterine structures by 2D ultrasound were enrolled,including 50 patients with endometrial polyps in the proliferative phase,51 cases with hyperplasia and 55 cases with normal histology or others.All were scheduled for hysteroscopy,dilatation and curettage,and the ultrasound was performed within 24 hours before the procedure. Endometrial volume, vascularity index (VI), flow index (FI) and vascularity flow index (VFI) were measured. These parameters were compared between endometrial polyps group and endometrial hyperplasia group.Results These parameters (endometrial volume,VI,FI and VFI) were all considered statistically significant between two groups.Conclusions Endometrial volume and 3D-PDI are good diagnostic tools in the differentiation between endometrial polyps and endometrial hyperplasia.
7.Plasma concentrations and phannacodynamics of fentanyl and morphine in postoperative patients using patient-controlled analgesia
Wei WANG ; Guoli ZHAO ; Xiaaying LIU
Chinese Journal of Anesthesiology 1994;0(06):-
Objective To determine the plasma concentrations and pharmacodynamics of fentanyl and nerphine used in postoperative epidural or intravenous patient-controlled analgesia (PCEA,PCIA) .Methods Sixty ASA Ⅰ - Ⅱ patients (36 male, 24 female), aged 18-25 yr undergoing elective major operation were randomly divided into 3 groups : group Ⅰ received PCEA with 0.13 % bupivacaine + fentanyl 2?g?ml-1 (n = 20) ; groupⅡ received PCEA with 0.13 % bupivacaine + morphine 0.08 mg? ml-1 ( n = 20); group Ⅲ received PCIA with morphine 0.5 mg ? ml-1 ( n = 20). In group Ⅰ and Ⅱ the background infusion rate was 4 ml ? h-1 , PCA bolus dose 2 ml and lock-out interval 20 min, while in group Ⅲ the back ground infusion rate was 1 ml?h-1 , PCA bolus dose 2 ml and lock-out interval 6 min. PCA was maintained for 48 h in all three groups. The vital signs, analgesic effect (VAS, VRS) and side-effects were recorded and venous blood samples were taken for determination of plasma fentanyl and morphine concentrations at 4 h, 24 h and 48 h after PCA was commenced. Results The demographic data were comparable among the three groups. There was no significant difference in MAP, HR and RR during PCA among the three groups. The analgesia was satisfactory in all three groups and no other analgesic was used during PCA. The rate of excellent analgesia ranged between 80%-85 % . The incidences of side-effects were higher in group Ⅱ and Ⅲ as compared with those in group Ⅰ . The volume of epidural PCA solution administered in 48 h was significantly larger in group Ⅰ than that in group Ⅱ( P
8.Protective effects of Huangqi against ischemia/reperfusion injury in rats
Wei WANG ; Qining LIU ; Xinjing ZHAO
Journal of Xi'an Jiaotong University(Medical Sciences) 2003;0(06):-
Objective To study the effects and mechanism of Huangqi against myocardium injury induced by ischemia and reperfusion in rats.Methods Totally 30 male Sprague-Dauley rats were randomly divided into three groups: control group(C group),ischemia/reperfusion group(I/R group),ischemic pretreatment with Huangqi group(H+I/R group),with 10 rats in each.The left anterior descending(LAD) coronary artery was ligated to establish the ischemia/reperfusion heart model.Huangqi was administered before the model was established in the H+I/R group.The pathologic changes of myocardial tissues(under light and electron microscopy),content of creatine kinase(CK),lactate dehydrogenase(LDH),malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) in serum,as well as activities of Na+K+-ATPase and Ca2+-ATPase in myocardial tissues were observed.Results Light and electron microscopic examination showed that the necrotic degeneration and pathologic changes of myocardiocytes in Huangqi group were significantly milder than those in the model group.The levels of CK,LDH,MDA were decreased significantly in Huangqi group(P
9.Optimization Study of MSCT Surestart Tracking and Triggering Technique for Imaging of Coronary Artery
Kai LIU ; Hongliang ZHAO ; Mengqi WEI
Chinese Medical Equipment Journal 2003;0(10):-
Objective To investigate the optimum threshold value of tracking and triggering technique (surestart) in imaging of coronary artery by 16-slice row CT. Methods 60 patients enrolled in the enhanced scan of coronary artery imaging were divided into 2 groups (group a : heart rate 70 beats/per minute ). The quality of images collected under different threshold value and heart rate was compared. The image quality of coronary artery was ranked by 3 levels, namely, level A, B and C. The data were statistically analyzed. Results The image quality triggered by 160 HU of group b was level A. The image quality triggered by 180 HU of group a was level A. Conclusion The optimum surestart is 180 HU when the heart rate 70 beats/per minute.
10.Hospital Infection in Respiratory Intensive Care Unit:Clinical Analysis and Prevention and Control Measures
Weihong XING ; Caihong LIU ; Wei ZHAO
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To determine the distribution of pathogens of bacterial infection in respiratory intensive care unit (RICU) to provide reference for the prevention of hospital infection. METHODS To collect specimens of the patients received endotracheal intubation from Jan 2006 to Dec 2006 in our RICU,to identify pathogens and drug sensitivity test. RESULTS We isolated 105 pathogenic bacteria from 626 specimens of the 58 patients,the G-bacilli accounted for 64.76%,G+ cocci accounted for 20.95%,fungi accounted for 12.38%; Acinetobacter baumannii was one of the main G-bacilli accounted for 19.05%. Staphylococcus aureus of G+ cocci for 9.52%,and Candida albicans of fungi for 7.62%; from 105 pathogens,65 from the respiratory tract,15 from the for urinary tract,eight from a catheter tube,six from the digestive tract,five from the blood,four from the various drainage tubes and two from the incision secretions. CONCLUSIONS Hospital infection pathogens exist in the respiratory tract infection in hospitalized patients of respiratory ICU with risk factors of age,underlying diseases,time in the intensive care unit,ventilator time and the use of invasive procedures in a certain relationship.