ive] To evaluate the therapeutic effect of Jiakang Ling (JL) for hyperthyroidism (HT) by ultrasonography. [Methods] Seventy-four cases of HT were randomly allocated to Group B ( n = 40, treated with JL) and Group C ( n = 34, treated with tabazol). And 20 healthy volunteers served as con trol (Group A). Ultrasonic parameters were observed in the three groups. [Results] Ultrasonic parame ters after treatment in 74 cases of HT were similar to those in 20 healthy volunteers. The differences of ul trasonic parameters between Group A and Group B were significant (P

Objective To study the quality of life and influencing factors in children with epilepsy.Methods The psychology of 96 children with epilepsy and 50 normal children were evaluated with the American quality of life and the affecting factors were analyzed.Results The quality of life of the epilepsy children was remarkably lower than that of normal children.The main presentation was seizure worry,side effects of drugs and difficulty of social intercourse.The age of initial presentation.the course of ease and so on affected quality of life in children with epilepsy(P＜0.05 or P＜0.01).The factors such as depression,family.environment,memory function,drug influence and seizure worry played important roles in decreasing order of importance.Conclusion QOL of children with epilepsy was remarkablely lower than normal children.General comprehensive care should be applied to children with epilepsy.Adequate drug treatment to control the seizure attack was very important.

AlM: To measure ocular biometric values with the degree of axial myopia and determine the relationship between the differences using the Lenstar 900.
METHODS:Totally 413 myopes (826 eyes) were enrolled in this study and were divided into 3 groups: low myopia (myopia <3. 00 diopters, 104 eyes), moderate myopia ( myopia =3. 00-6. 00 diopters, 500 eyes) and high myopia ( myopia > 6. 00 diopters, 222 eyes ). Central corneal thickness ( CCT ) , aqueous depth ( AD ) , lens thickness (LT), axial length (AL) were measured by Lenstar 900. The parameters were tested using analysis of variance and the relations among SE, AL, LT were analyzed.
RESULTS: There were significant difference both in AL (F=206.16, P<0.01) and AD (F=4.764, P<0.05) and no significant difference both in CCT and LT between these myopia groups. With analysis of Person, the Spherical equivalent ( SE) shows a significantly positive correlation with AL (r=0. 662, P<0. 01) and AD (r=0. 095, P<0. 05), no correlation with CCT and LT. AL shows a significantly positive correlation with AD (r=0. 347, P<0. 01) and CCT (r=0.126, P<0.01), negative correlation with LT (r=-0.265, P<0. 01). LT shows a significantly negative correlation with AD (r=-0. 496, P<0. 01).
CONCLUSlON: Along with the diopters increasing in myopia, the axial length and aqueous depth continue to increase. Spherical equivalent ( SE) shows a significantly positive correlation with AD. AL shows positive correlation with SE, AD and CCT and negative correlation with LT. LT shows a significantly negative correlation with AD.

The practice and exploration of bilingual teaching for the course of microbiology has been made in order to improve the students foreign lingual level and to meet the higher requirement on tip-top person with the social development. As a result,bilingual teaching is welcome,and the teaching effect is so distinct that the aim was reached to either study the fundamental knowledge or enhance the English level.

ObjectiveTo study the effects of using bilingual education among different nursing undergraduates in the Nursing Fundamentals, and then make out some proper education pattern. Methods Bilingual education has been implemented for one year in the course of Nursing Fundamentals among 82 nursing undergraduates who have graduated from high school and 81 nursing undergraduates who have graduated from hygiene school in 2003 degree. Results There were significant difference between two kinds of nursing undergraduates in the passed rate of CET 4, P0.05. Conclusion The bilingual education can be implemented in the different nursing undergraduates.

OBJECTIVETo compare clinical therapeutic effects of acupuncture, moxibustion, acupuncture plus moxibustion at Weiwanxiashu (EX-B 3) on diabetes.

METHODSSeventy-nine cases of type 2 diabetes were randomly divided into 3 groups: acupuncture group (A), moxibustion group (B) and acupuncture plus moxibustion group (C) and Weiwanxiashu (EX-B 3) was selected in the 3 groups. The main clinical symptoms, fasting blood glucose, 24 hurinal glucose, glycosylated hemoglobin, and blood lipids were investigated and compared before and after treatment.

RESULTSAfter treatment, the clinical symptoms significantly improved; fasting blood glucose, 24 h-urinal glucose, glycosylated hemoglobin, and cholesterol, triglyceride, low density lipoprotein all decreased in varying degrees, and the high density lipoprotein increased (P < 0.05) in all of the 3 groups, with the effects being the best in the acupuncture plus moxibustion group.

CONCLUSIONAcupuncture, moxibustion, or acupuncture plus moxibustion at Weiwanxiashu (EX-B 3) is an effective therapy for diabetes, and the acupuncture plus moxibustion has a better result.

Acupuncture Points ; Acupuncture Therapy ; methods ; Adult ; Aged ; Blood Glucose ; analysis ; Diabetes Mellitus, Type 2 ; therapy ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; methods

A method of comprehensive chemical pattern recognition of Atractylodis Rhizoma was established by GC-MS fingerprint, principal component analysis, cluster analysis and discriminant analysis. A DB-wax column (0.25 mm x 60 m, 0.25 microm) with El ion source and 70 V electron multiplier were used for GC-MS analysis. Using principal component analysis, cluster analysis, and discriminant analysis, 15 common peaks of sample fingerprints for chemical pattern recognition research were analysed. The same results were obtained from the fingerprint, principal component analysis and cluster analysis, which could use to distinguish genuine Atractylodes lancea, ungenuine A. lancea and A. chinensis. Thus, this method could be used for the quality control and comprehensive evaluation of Atractylodis Rhizoma.
Atractylodes ; chemistry ; China ; Discriminant Analysis ; Drugs, Chinese Herbal ; chemistry ; Gas Chromatography-Mass Spectrometry ; methods ; Quality Control ; Rhizome ; chemistry

OBJECTIVETo investigate the association between HPV16 infection, E6/E7 variations and the cervical lesions.

METHODSHPV subtypes were detected by using flow-through hybridization technique, E6/E7 gene was extracted from cervical lesions in 80 patients with HPV16 infection, PCR amplified, cloned into plasmid pMD18-T and sequenced.

RESULTSHPV 16 was the most common type which accounted for 33.3% (154/463), the HPV16 infection rates increased with the severity of cervical lesions (P < 0.05). Totally in 72 cases the complete E6 and E7 regions were successfully sequenced, the DNA mutation rate of E6/E7 was 88.9% (64/72). A mutation, E6-D32E (T96G) coincided with a specific type of E7 mutation, N29S (A86G). D32E/N29S mutation rate was 38.9% (28/72), the detection rate increased with the severity of cervical lesions (P < 0.05).

CONCLUSIONHPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.

Adult ; Aged ; China ; epidemiology ; Female ; Gene Frequency ; Genotype ; Host-Pathogen Interactions ; Human papillomavirus 16 ; genetics ; physiology ; Humans ; Middle Aged ; Mutation ; Oncogene Proteins, Viral ; genetics ; Papillomavirus E7 Proteins ; Papillomavirus Infections ; epidemiology ; pathology ; virology ; Repressor Proteins ; genetics ; Severity of Illness Index ; Uterine Cervical Diseases ; epidemiology ; pathology ; virology ; Uterine Cervical Neoplasms ; epidemiology ; pathology ; virology ; Young Adult

OBJECTIVETo investigate the effects of Nocardia rubra cell wall skeleton (Nr-CWS) on the HeLa cell line, one of the cell lines of human cervical cancer, infected with HPV.

METHODSHPV-infected HeLa (HPV 18-positive cells) cultured in vitro were divided into two groups: the experiment group and control group. Nr-CWS was added to the experiment group and PBS to the control. The growth and proliferation of HeLa cells were detected with MTT and flow cytometry technology. Inhibitive effect of HeLa transplanted tumor was investigated in Scid mice.

RESULTSThe growth of HeLa cells in the experimental group was apparently decreased compared with that of the control. The results of flow cytometry demonstrated that more HeLa cells were transferred into quiescent phase in the experimental group than that in the control. While less in the proliferative phase, both of the volume and weight of HeLa transplanted tumor with drug-added group were less than those of control group.

CONCLUSIONThe Nocardia rubra cell wall skeleton is a potiental growth inhibitor and inducer of apoptosis of cervical cancer cells in vitro and may provide a new way in prevention or supplementary management of anti-human papilloma virus.

Animals ; Cell Growth Processes ; drug effects ; Cell Survival ; drug effects ; Cell Wall Skeleton ; pharmacology ; therapeutic use ; Female ; Flow Cytometry ; HeLa Cells ; Host-Pathogen Interactions ; Humans ; Mice ; Mice, SCID ; Nocardia ; metabolism ; Papillomaviridae ; physiology ; Uterine Cervical Neoplasms ; pathology ; prevention & control ; virology ; Xenograft Model Antitumor Assays

Objective To discuss the significance of HPV16, 18,31,33 subtype infection in cervical cancer screening for women whose age is beyond 30. Methods The patients who choose the combination use of TCT and HPV-DNA genotyping for screening during Jan. 2008-Mar. 2009 in our Gyn.Out-patient department, for that HPV16,18,31,33 positive ones, colposcopy and biopsy were used for further examination to get the histopathology result. Results There were 275 patients in total who suffers HPV16,18,31,33 infection, their age ranges from 30-72, the average age is 41.76 ± 8.88; HPV 16 infection accouts for 64.0%, one single subtype infection accouts for 64.7%; Among 210 ones who have an abnormal cytology test result, 127(60.5%)were confirmed to be CIN Ⅱ + by histopathology. In patients whose cytology test result were ASC-US,ASC-H,LSIL, HSIL,SCC and AGC, there were 32.6% ,67.8% ,42. 3%,81.8% , 100. 0%, 50.0% P = 0.000) of them who were confirmed to be CIN Ⅱ + by histopathology separately;There were 65 ones whose cytology result is negative, 10 patients were certified of CIN Ⅱ , Ⅲ by histopathology(15.4%) ,it accounts for 7.3% of all patients whose histopathology result is advanced of CIN Ⅱ,Ⅲ, 9 were found in HPV 16's group. Conclusion HPV16, 18,31,33 subtype infection have a significance in cervical cancer screening program for women whose age is beyond 30. We can detect another extra 7.3% CIN Ⅱ , Ⅲ ,especially in HPV16's group.