1.Content Determination of Residual Organic Solvents in Artesunate by Capillary GC
Hongying SUN ; Aili ZHAO ; Zhenguang LIN
China Pharmacy 2005;0(19):-
OBJECTIVE:To establish capillary GC method for the determination of residual organic solvents in artesunate,such as methanol and ethyl acetate.METHODS:Capillary GC was adopted and the temperature of flame ionization detector was 250℃.RESULTS:The linear range were 0.03~0.605 mg?mL-1 for methanol(r=0.999 8) and 0.05~1.002 mg?mL-1 for ethyl acetate(r=0.999 7).The average recovery rates were 98.7% for methanol(RSD=3.0%) and 99.1% for ethyl acetate(RSD=2.1%).Only the ethyl acetate was detected in samples.CONCLUSION:Established method is simple and accurate for the content determination of residual organic solvents in artesunate.
2.Magnetic Resonance Imaging of Intracranial Hemorrhage
Lin SUN ; Guozhong LI ; Yanfeng ZHAO
International Journal of Cerebrovascular Diseases 2006;0(02):-
Traditional sequences of magnetic resonance imaging (MRI) such as T1-weighed imaging, T2-weighed imaging, and fluid-attenuated inversion recovery (FLAIR) imaging can sensitively detect the subacute and chronic intracranial hemorrhage; gradient echo sequence can detect various stages of intracranial hemorrhage, but there is no correlation between the signal intensity of the hematoma and the time of the presence of hematoma. Diffusion-weighted imaging and apparent diffusion coefficient may provide more information about the center and surrounding areas of hematoma, as well as the hemorrhagic tendency after infarction.
3.Analysis of the structure and function of the extraocular muscle connective tissue in rabbit
Chunhua, SUN ; Kanxing, ZHAO ; Jinyong, LIN
Chinese Journal of Experimental Ophthalmology 2014;32(6):518-520
Background The extraocular muscles (EOMs) Pulley in primate is related to the function of EOMs,but there are arguments about that.The location of rabbit's eyes is different from the primate.Study on the structure of the extraocular muscle connective tissue in rabbit may play a role to analyze the function of the EOMs Pulley.Objective The structure of the connective tissue around EOMs in rabbit was studied,the difference between this structure in rabbit and homan' s EOMs Pulley in past reports was analyzed,and then,the role of EOMs Pulley to ocular movement was investigated.Methods Five adult rabbits were involved.The gross anatomy of an orbit in each rabbit was observed.The other orbit was processed with paraffin imbedding and coronal serial sections.A murine monocolonal antibody to α-smooth muscle actin(α-SMA) was used to show the smooth muscle,while Weigert stain was used to show muscle and collagen,and Masson trichrome stain to show the elastin.Results An encircling ring of collagen circled every EOM.The collagen ring was thin and connected to the orbital layer muscle fiber loosely.Collagen tissues coupled to adjacent EOMs.Less elastin fibers and scanty the smooth muscle cells were embedded in the collagen.Conclusions The connective tissue around rectus in rabbit is different from that in human with developed binocular ocular movements.It shows that connective tissue around EOMs may be related to the function of ocular movements.
4.The Learning style and appropriate teaching method for medical students
Lin ZHAO ; Hongbin ZHANG ; Yanchun SUN
Chinese Journal of Medical Education Research 2006;0(10):-
Based on the survey of the learning preference of Kunming Medical College students,we analyze relationship between students’learning style and personalities.In order to match different learning style,teachers must find some strategies in their English teaching.
5.Interventional therapy of complications after liver transplantation:hepatic artery stricture
Lin-Sun LI ; Hai-Bin SHI ; Lin-Bo ZHAO ;
Journal of Interventional Radiology 2006;0(10):-
Hepatic artery stricture (HAS) after liver transplantation can lead directly to transplanted liver function exhaustion and complications of biliary system. The early diagnosis and treatment are crucial for better prognosis. Doppler ultrasound is the first method of choice, and angiography can give further clear dignosis. The balloon dilatation is still effective for hepatic arterial stenosis. With the more adaptable usage of oronary stent, if possible, would reveal more promising result especially for tortuous stenotic hepatic artery. The vascular reconstruction or repeated liver transplantation is still the effective therapeutic methods.
6.Twenty-seven cases of urinary retention after stroke treated by point-application combined with acupuncture.
Shi-Lin SUN ; Zhong-Ren SUN ; Zhao-Xia GUO
Chinese Acupuncture & Moxibustion 2012;32(10):933-934
Acupuncture Points
;
Acupuncture Therapy
;
Adult
;
Aged
;
Drugs, Chinese Herbal
;
therapeutic use
;
Female
;
Humans
;
Male
;
Middle Aged
;
Stroke
;
complications
;
Urinary Retention
;
drug therapy
;
therapy
7.Acupuncture and massage therapy for 23 cases of pharyngitis caused by cervical vertebra disease.
Xiang-Qian LIN ; Hong-Yi ZHAO ; Qing SUN
Chinese Acupuncture & Moxibustion 2012;32(2):166-166
Acupuncture Therapy
;
Adult
;
Cervical Vertebrae
;
injuries
;
Combined Modality Therapy
;
Female
;
Humans
;
Male
;
Massage
;
Middle Aged
;
Pharyngitis
;
etiology
;
therapy
;
Spinal Diseases
;
complications
;
Young Adult
8.Effect of hepatitis C virus genotype on antiviral therapy in patients with human immunodeficiency virus/hepatitis C virus coinfection
Zhaoyun CHEN ; Yan SUN ; Qingxia ZHAO ; Chaofeng LI ; Lin DING
Chinese Journal of Infectious Diseases 2017;35(7):403-406
Objective To investigate the effect of hepatitis C virus (HCV) genotype on antiviral therapy in patients with human immunodeficiency virus (HIV)/HCV coinfection in Henan province.Methods A total of 129 patients were coinfected with HIV and HCV, among whom, 70 were HCV 1b genotype and 57 HCV 2a genotype.And 131 patients were HIV single infection.Immunological failure rate, virological suppression, CD4+ T lymphocyte counts and liver and renal function after antiretroviral therapy (ART) were compared among the three groups.Flow cytometry was used to count CD4+ T lymphocytes and polymerase chain reaction amplification was used to detect HIV RNA.The liver and renal function were tested by automatic biochemical analysis.Statistical analysis was conducted by χ2 test, analysis of variance and LSD-t method.ResultsImmunological failure rate in HCV 1b group, HCV 2a group and HIV single infection group were 7.14% (5/70), 15.79% (9/57) and 9.92% (13/131), respectively.There was no significant statistical difference among the three groups (χ2=2.59, P>0.05).The CD4+ T lymphocyte counts in three groups were (614±258), (529±245), and (518±243) cells/μL, respectively.The difference was statistically significant (F=3.17, P<0.05).The virus inhibition rates of three groups were 87.0% (HCV 1b), 78.2% (HCV 2a), and 82.3% (HIV single infection).The HIV virus failure rates were 8.6% (HCV 1b), 14.5% (HCV 2a), and 13.1% (HIV single infection).There was no significant difference among three groups (χ2=1.967, P>0.05).The levels of aspartate transaminase, alanine aminotransferase and total bilirubin in HCV 1b group and HCV 2a group were all significantly higher than those in HIV single infection group (F=27.38, 15.22 and 7.33, respectively, all P<0.05), while there was no significant difference between HCV 1b and HCV 2a groups (t=1.27, 0.29 and 1.59, respectively, all P>0.05).Conclusions The main HCV genotypes in patients with HIV/HCV coinfection by blood transmission are HCV 1b and HCV 2a in Henan province.HIV/HCV coinfection does not affect the effect of ART, but could aggravate the liver damage in acquired immune deficiency syndrome patients.
9.Detection and clinical significance of blood D-Dimer in rheumatoid arthritis
Jinxia ZHAO ; Lin SUN ; Xia ZHANG ; Xiangyuan LIU
Chinese Journal of Rheumatology 2011;15(3):168-171
Objective To detect D-Dimer in the blood of rheumatoid arthritis (RA) patients and to investigate its clinical significance in RA. Methods Blood samples were obtained from 58 patients with RA,18 patients with systemic lupus erythematosus (SLE), 15 patients with ankylosing spondylitis, 11 patients with osteoarthritis, and 20 patients with other connective tissue diseases. The presence of thrombotic diseases was excluded in all patients. The presence of D-Dimer in the blood was examined by immunoturbidimetry. The following clinical and laboratory data were collected: disease activity index DAS28, rheumatoid factor (RF),erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP). Mann-Whitney U test, t-test ,Spearman correlation were used for statistical analysis. Results ① The prevalence of high level D-Dimer in RA patients was higher than that of the control group (82.6% vs 21.9%, P<0.01). The titer of D-Dimer in RA was significantly higher than that of the control group [(1.76±1.57) vs (0.32±0.25) mg/L, P<0.01]. ②DAS28 was higher in RA patients with positive D-Dimer than those with negative D-Dimer (5.4±1.0 vs 4.4±0.8,P<0.01). The values of ESR, CRP and RF in RA patients with positive D-Dimer were significantly higher than those in patients with negative D-Dimer (P<0.05). ③ There was positive correlation between D-Dimer and DAS28 (r=0.406, P<0.05), ESR (r=0.355, P<0.01), and RF (r=0.319, P<0.05). Conclusion The level of D-Dimer in the blood of RA patients is significantly higher than other rheumatic diseases, and is positively correlated with disease activities. The results indicated that the activation of coagulation-fibrinolytic systems may play a role in the pathogenesis of rheumatoid arthritis.
10.Clinical analysis of 86 patients with spondyloarthropathy associated with anterior uveitis
Rui LIU ; Xiangyuan LIU ; Jinxia ZHAO ; Lin SUN
Chinese Journal of Rheumatology 2010;14(11):762-765
Objective To investigate the clinical features and risk factors of spondyloarthropathy with anterior uveitis. Methods The clinical and laboratory data of 86 patients with spondyoarthropathy associated with anterior uveitis in our hospital were collected, analyzed and summarized from March 2005 to December 2008, and the patients were followed up as closely as possible. The data of the 86 patients were compared with 93 patients who had spondyloarthropathy without anterior uveitis at the same period. All data were analyzed by using SPSS11.5 software package. Results Compared with non-ophthalmia group, ophthalmia group had significantly longer course[(11 ±8)vs(5±6), P<0.01], and higher proportion of positive family history(27.9%vs 9.7%, P<0.01), the proportion of low back pain at night, morning stiffness, spinal deformity, limitation of waist-bending and severe sacroiliac joint lesions were all significantly higher(P<0.05), HLA-B27 positive rate was significantly higher as well(92.2% vs 81.5%, P<0.05). The attack of uveitis usually had seasonality and precipitating cause. The patients with anterior uveitis as first symptom had significant higher frequency of ophthalmitis(P<0.01), the ratio of eye permanent lesions was also significantly higher(P<0.01). The frequencies of attack were positively correlated with the course of disease(r=0.294, P=0.006), Logistic multiple regression analysis showed that the incidence risk of ophthalmia were related to the course of disease(P=0.013, OR=1.099, 95%CI 1.030~1.183)and severe sacroiliac joint lesions(P=0.012, OR=3.071, 95%CI 1.286 ~7.314). Conclusion The spondyloarthropathy associated with anterior uveitis had its own characteristics, We should pay attention to the risk factors of anterior uveitis,and prevent the recurrence of ophthalmia.