Objective To construct a recombinant adenovirus contained respiratory syncytial virus F gene fragment. Methods The F gene fragment was amplified by RT-PCR. Then the recombinant shuttle plasmid pShuttle-CMV/F was constructed. The iinearized pShuttle-CMV/F was transformed into BJ5183-AD-1 electroporation competent cells which contained plasmid pAdEasy-1. The obtained homologous recom-binant plasmid was named pAdEasy/F, and transfected into 293 cells. Then the recombinant adenovirus rAd/F was obtained, and it was tested by electron microscope, RT-PCR, Western blot and immuno-fluores-cence assay(IFA). The vires titer and genetic stability were also studied initially. Results The recombi-nant adenovirus rAd/F was constructed, which was visualized as typical adenovirus morphology under elec-tron microscope. The transcription and expression of RSV F gene fragment integrated in the rAd/F were con-finned by RT-PCR, Western blot and IFA. Conclusion The recombinant adenovirus rAd/F contained RSV F gene fragment is successfully constructed and lay an important foundation for the further vaccine study.

OBJECTIVE:To prepare in-situ gelling of hydrochloride sparfloxacin eye drops and establish its quality con-trol method.METHODS:The in-situ gelling of hydrochloride sparfloxacin eye drops were prepared with boric acid as buffer to regulate the pH and osmotic pressure and with sodium polymannuronate as peptizer.The content of hydrochloride sparflo-xacin was determined by ultraviolet spectrophotometry.The stability of the sustained-release eye drops was investigated as well.RESULTS:The sustained-release eye drops were colorless or yellowish limpid liquid with its test and identification results all in conformity with the related stipulation stated in Chinese Pharmacopeia(2005 edition).There was a good linear relationship within the concentration range of 3.0～8.0 ?g?mL-1 for hydrochloride sparfloxacin,and its average recovery was 99.82%(RSD=0.236%,n=6).There was no significant change for indexes in the accelerate test and sample test,yet the temperature and lighting did have slight impact on the viscosity,osmotic pressure and pH of the solution.CONCLUSION:The preparative method is simple and feasible and the quality of in-situ gelling of hydrochloride sparfloxacin eye drops is stable and controllable.

Objective To investigate the dual-direction regulation of Tongxie Yaofang on gastrointestinal movement by studying its effect on gastrointestinal movement of mice in various functional states. Methods Adopting diarrhea model induced by rhubarb,constipation model by compound diphenoxylate and normal mice,the effect of different doses of Tongxie Yaofang on loose stool quantity,degree,diameter,diarrhea rate,loose stool incidence rate,loose stool grade,diarrhea index of every group were observed. By esta blishing hyperfunction of small intestinal function in mice by neostigimine,inhibition of small intestinal function in mice by atropine sulfate and small intestinal propellant function in normal mice,the effect of Tongxie Yaofang on intestinal movement of mice were studied. Results Tongxie Yaofang could inhibit gastrointestinal movement of diarrhea model and hyperfunction of small intestinal function in mice by neostigimine. It could promote gastrointestinal movement of constipation model and inhibit small intestinal function in mice by atropine sulfate. But it had no obvious action on natural mice and its intestinal movement. Conclusions Tongxie Yaofang has dual-direction regulation on gastrointestinal movement of mice in various functional states,which has a good agreement with formula-syndrome in traditional Chinese medicine,conforms to treatment based on syndrome differentiation.

1-Alkyl-2-acetylglycerophosphocholine Esterase ; blood ; genetics ; Adolescent ; Asthma ; blood ; enzymology ; genetics ; Child ; Child, Preschool ; Female ; Gene Expression Regulation, Enzymologic ; Genotype ; Humans ; Infant ; Male ; Mutation ; Polymerase Chain Reaction

Motion segment and extraction from continuous signals is the premise of surface electromyography (sEMG) analysis. For the problem that sEMG energy threshold required repeated manual testing to set, this the paper established a this mathematical model of continuous actions based on Gaussian sEMG energy curve, in which the energy threshold was set according to the distribution of Gaussian signal section, and differentiated the action signals from no-action signals combined with energy comparison method. The experiment results showed the method can achieve continuous repetitive action segmentation, and compared with manual segmentation of the same signal, has a higher degree of coincidence.
Algorithms ; Electromyography ; Humans ; Motion ; Pattern Recognition, Automated ; Signal Processing, Computer-Assisted

Aim To observe the anti-apoptotic effect of different concentrations of amygdalin on the endplate chondrocytes induced by IL-1 βderived from rat inter-vertebral discs and explore the possible mechanism fur-ther.Methods Chondrocytes were obtained from endplate of one-month SD rat intervertebral discs and cultured primary chondrocytes.After identifying,they were divided into normal group,induced group and A-mygdalin 1 0 -2 mol·L -1 ,1 0 -3 mol·L -1 ,1 0 -4 mol· L -1 ,1 0 -5 mol · L -1 administration group.Then the apoptosis was detected by flow cytometry (FCM).Re-al-Time PCR was adopted to detect the mRNA expres-sion of Bax and Bcl-2.The protein expression of Bax and Bcl-2 was detected by Western blot.Results The apoptosis of the endplate chondrocytes induced by IL-1 βderived from rat intervertebral discs could be inhib-ited by amygdalin with different concentrations.Amyg-dalin could reduce the apoptotic rate analysed by FCM,down-regulate the Bax mRNA expression of Bax and up-regulate the Bcl-2 mRNA assayed by RT-PCR;compared with the induced group the differences were statistically significant (P <0.05).Besides,observa-tion of the protein expression of Bax and Bcl-2 by Western blot found that amygdalin 1 0 -4 mol · L -1 could inhibit the effect of IL-1 β,which could increase the protein expression of Bax and reduce the protein expression of Bcl-2.Conclusion Amygdalin has an effect on anti-apoptosis of the end-plate chondrocytes induced by IL-1 βderived from rat intervertebral discs and improve the degeneration of intervertebral discs.

Objective To discuss the efficacy and safety of retroperitoneal laparoscopic ureterolithotomy in treatment of ureteral calculi.Methods All 33 cases with upper or midst ureter calculi were treated by laparoscopic ureterolithotomy,ESWL or URSL ineffective for 26 cases.The diameters of calculi ranged from 0.7 to 2.1 cm.Results All the operations were successful.The operation time was 30～120 min with a mean of 55 min.The intraoperative bleeding volume Was 40 ml (20～80 ml) on average no complication Was observed duing the follow-up of 6～12 months.All the hydronephrosis Was alleviated.Conclusion Laparoscopic ureterolithotomy is suitable for ureteral calculi treated unsuccesfully by open surgery.It is effective,safety and miniinvasive.

OBJECTIVE To study the relationship between potassium channel and ADAR1 in Hep-2 cell line. METHODS The potassium current was recorded by the whole-cell recording technique of perforated membrane clamp. Using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) , the expressions of ADAR1 mRNA in the Hep-2cell line were detected. RESULTS The similar membrane current was observed when cells were held at -40 mV and test potentials ranged from -80 mV to +80 mV. The current exhibited properties of voltage-dependent, outward rectification. It exhibited complete activation after alatency of 25ms and no or little inactivation over the 800ms voltage pulse. It could be blocked by potassium channel blockers TEA. The relative intensities of ADAR1 mRNA of t he Hep-2 cell line were different before and after its potassium channel was blocked. CONCLUSION Delayed rectifier potassium channel exist in human laryngeal carcinoma cell line Hep-2.The potassium channel is voltage-dependent. There is a correlation between the potassium channel and ADAR1 mRNA in Hep-2 cell line.

OBJECTIVE:To facilitate the hierachical management and use of antibiotics and tighten control on prescription right on the narcotics drugs.METHODS:Hospital informational system has contributed to the informatization management on supervision of the prescriptions of antibiotics and narcotics.RESULTS:After half a year's practice of informatization management,remarkable outcome has achieved in that the practice of hierachical management and use of antibiotics has been improved significantly,and the management on the prescription right of narcotics drugs became more simple and reliable.CONCLUSIONS:Adopting hospital information system to tighten control on the prescriptions of antibiotics and narcotics drugs is a good management model in which various abnormal prescription behaviors can be effectively warned of and guard against from the very beginning.