5.Biocompatibility of bone marrow mesenchymal stem cells with bladder acellular matrix scaffold
Xiaojun ZHAO ; Jun YU ; Yingfei DUAN
Chinese Journal of Tissue Engineering Research 2015;(36):5769-5773
BACKGROUND:In the repair of urinary tract defects, we have been actively trying to construct the urinary tract substitutes with normal physiological function through combining ideal seed cel s and proper scaffold materials by tissue engineering method. OBJECTIVE:To investigate the biocompatibility of bone marrow mesenchymal stem cel s with rabbit bladder acel ular matrix scaffold. METHODS:Rabbit bone marrow mesenchymal stem cel s were isolated and cultured using density gradient centrifugation method. Passage 3 rabbit bone marrow mesenchymal stem cel s were cultured on the rabbit bladder acel ular matrix. The cel s were counted every day for 12 days, to drawn a cel growth curve. Bone marrow mesenchymal stem cel s cultured alone were used as control group. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y seeded onto the bladder acel ular matrix. Under the inverted microscope, the cel s grew out of the bladder acel ular matrix, and a great amount of long spindle-shaped cel s were found around the bladder acel ular matrix. With 5 days of inoculation, the cel s in the two groups grew gently;at 6-9 days, the cel growth curve gradual y became steeper, and the cel division and growth were increased exponential y;at 10-12 days, the cel s recovered to a gentle state. Cel growth curves in the two groups were basical y coincident, suggesting that rabbit bone marrow mesenchymal stem cel s have good biocompatibility with the bladder matrix.
6.The myocardial injury after limb ischemia/reperfusion in rats and the protective effects of NO on myocardium.
Li-Jun ZHAO ; Guo-Xian DUAN ; Shu-Yun DONG
Chinese Journal of Applied Physiology 2007;23(3):268-327
Animals
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Extremities
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blood supply
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Ischemia
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metabolism
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Male
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Myocardial Reperfusion Injury
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metabolism
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Nitric Oxide
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blood
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Oxidative Stress
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Rats
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Rats, Wistar
7.The Comparison of Radiography and CT in the Diagnosis of Acetabular Fractures
Xiaolin HU ; Dong ZHAO ; Minjiang DUAN ; Jun WU
Journal of Practical Radiology 2001;0(07):-
Objective To evaluate the applicable and clinical value of CT and radiography in the dignosis of acetabular fractures.Methods The data of CT,X-ray and clinical treatment in 18 cases with acetabular fractures were analyzed and comparatively.Results There were 4 cases of the anterior wall fracture,4 cases of the anterior column fracture,2 cases of the posterior wall fracture,2 cases of the posterior column fracture and ,6 cases of complex fracture,11 cases were in company with swelling of the soft tissue in pelvic cavity.Of them,4 cases misdiagnosed by radiography.27 pieces of bone fragment were detected by CT,including 17 pieces of them in joint cavity.12 pieces of bone fragment were detected by X-ray,only 4 pieces of them were in joint.3 cases with femoral head fracture were included in 7 cases of the hip joint dislocation,1 case of them was misdiagnosed by X-ray.Conclusion In detecting the bone fragments in articular space,the type of acetabular fractures and soft-tissue swelling,CT is super to radiography.
8.Effects of SREBP-1 targeted RNAi on lipid droplet formation in HKC cells under stimulation of high glucose
Jun HAO ; Hong SHI ; Song ZHAO ; Yunzhuo REN ; Huijun DUAN
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To construct eukaryotic expression vector of shRNA(small hairpin RNA)for human SREBP-1(sterol regulation element binding protein-1)gene and explore its effects on lipid droplet formation in human renal proximal tubular epithelial cell line(HKC)under the stimulation of high glucose.Methods Two eukaryotic expression vectors of shRNA were constructed for human SREBP-1 gene.The HKC cells were transfected with negative control plasmid(pGenesil-1-HK)and two recombinant vectors(pGenesil-1-SREBP1-1 and pGenesil-1-SREBP1-2)and then were cultured under the stimulation of high glucose for about 48 h.The expression of SREBP-1 mRNA and FAS mRNA was detected by RT-PCR and SREBP-1 protein expression was investigated by Western blot.Lipid droplets were detected by Oil Red O staining.Results DNA sequencing showed that the target segments were successfully cloned into pGenesil-1 vector respectively.RT-PCR indicated that two recombinant vectors could inhibit the expression of SREBP-1 mRNA and FAS mRNA in HKC cells under the stimulation of high glucose.Similarly,SREBP-1 protein was also inhibited by the transfection with recombinant vectors.Oil Red O staining found that silencing of SREBP-1 gene resulted in lipid droplets decrease.Conclusions The eukaryotic expression vector of shRNA for human SREBP-1 gene was successfully constructed,and the expression of SREBP-1 was inhibited effectively by the expressed siRNA in HKC cells that resulted in lipid droplets decrease through FAS mRNA transcription inhibition.
9.Application of Luminex xMAP technology in infectious diseases.
Chinese Journal of Virology 2010;26(2):158-161
Communicable Diseases
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diagnosis
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Humans
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Influenza A Virus, H1N1 Subtype
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genetics
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Influenza, Human
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diagnosis
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virology
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Microspheres
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Nucleic Acid Amplification Techniques
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methods
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Nucleic Acid Hybridization
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methods
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Oligonucleotide Probes
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genetics
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Polymerase Chain Reaction
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methods
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Sensitivity and Specificity
10.The significance of NO in renal cell apotosis following hind limbs ischemia/reperfusion in rats.
Guo-xian DUAN ; Lian-yuan ZHAG ; Li-jun ZHAO
Chinese Journal of Applied Physiology 2009;25(2):281-285
Animals
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Apoptosis
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Extremities
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blood supply
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Kidney
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pathology
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Male
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Nitric Oxide
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physiology
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Random Allocation
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Rats
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Rats, Wistar
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Reperfusion Injury
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pathology
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physiopathology