This paper discussed the selection of SCI journals and questions associated with submitting manuscripts.The strategy for the submitting manuscripts.and the problems in the publication of manuscripts were also summarized in this article.These results would be valuable and guide for the researchers to submit their manuscripts,conform the writing skills of scientific papers,and improve the efficiency of SCI publications.

objectives To provide the reference for the Research & Development departments of hospitals by analyzing the quantity of publications and their cited status for the top 20 hospitals in SCl and MEDLINE.and top 100 hospitals in domestic publications in 2006.Methotis The number of publications and their cited times for the first 20 hospitals in SCI and MEDLINE.and the top 100 hospitals in domestic publications in 2006 were analyzed with bibliometric analysis.Results In 2006.the average increased ratio of publications in SCI for top 20 hospitals was 47.58%.but the number of articles in MEDUNE WaS decreased.For top 100 hospitals.the increased number of domestic publications was 71.25,the number of cited publications WaS increased 160.14.Conclusions The SCI publications from top 20 hospitals increased sharply,but publicatiOils indexed by MEDLINE decreased respectively.

Objective To evaluate the academic level and the popularity of Chinese Journal of Laboratory Medicine.Method With Chinese Medical Citation Index(CMCI), we analyzed the amount and distribution of the originals in Chinese Journal of Laboratory Medicine cited by the journals indexed in CMCI.Result The percent of cited papers in all published articles was 55.68% during the period, the average number of cited times is 4.83, the top cited value was 108.206 articles (30.03% of total papers) were cited 5 or more, and the all cited times from them were 2428(73.33% of total cited times). 686 cited articles were written by 526 authors from different countries.The most frequently cited author wrote 12 papers, the authors with one paper cited were 430(81.75% of total authors).The cited authors are from the 31 provinces and other country; Beijing, Zhejiang and Shanghai are in the front of Laboratory Medicine research.Beijing was the top of rank (44.40% of total authors).292 institutes were cited and the most frequently cited institute was Beijing Hospital(10.48% of total cited times).There were 563 citing journals where the self-citing rate is 9.85%.Conclusion The Chinese Journal of Laboratory Medicine has published high quality articles. It is the one of the most important information resource for the laboratory medicine researchers and the most important medical journal.

Objective To provide references to NSF funding policy and planning based on the grant project analysis.Methods Funding grants of NSF Distinguished Young Scholars in medical field during 2011-2015 were collected,and compared with the data of 2001-2010.Indicator data was analyzed including grant distribution in supporting institutes,geography locations and medical subject.Results In recent 5 years,the number of application for the grant is increasing,while the funding number stays the same.The universities governed by Ministry of Education and China Academy of Science are the primary grantee institutions.Beijing,Shanghai,and other developed areas got more grants than other provinces.Most grants were focused on oncology,neurology and psychology,medical imaging and medical engineering,and pathogen biology and infection.Conclusions The funding number of NSF supporting for Distinguished Young Scholars should be incresed with applications increasing.The supporting covers in areas,institutes and medical subjects should be extensively.

Objective:To observe the therapeutic effect and timing of reteplase in patients with ST-segment elevation myocardial infarction (STEMI) .Methods:A total of 106 STEMI patients were selected from Central Hospital of Wafangdian City of Liaoning Province , from Jan 2013 to Apr 2014 ,and all patients received thrombolytic treat-ment .According to the duration from onset to thrombolysis ,patients were divided into :group 1 with duration <3h (<3h group ,n=28) ,group 2 with duration 3~6h (3~6h group ,n=47) ,group 3 with duration 6~12h (6~12h group ,n=22) and group 4 with duration 12~24h combined sustained chest pain (12~24h group ,n=9) .Thrombo-lytic recanalization rate ,30d all-cause mortality and incidence rate of adverse reactions were observed in all groups . Results:Compared with group 1 , 2 , the thrombolytic recanalization rate significantly reduced (82.14% vs . 76.60% vs .50.00% vs .44.44% ) in group 3 ,4 (P<0.05 all) ,but there was no significant difference between group 3 and group 4 ;Compared with group 1 , the 30d all-cause mortality (3.57% vs .6.38% vs .9.09% vs . 11.11% ) and incidence rate of adverse reactions (3.57% vs .10.64% vs .13.64% vs .22.22% ) in group 2~4 (P<0.05 all) ,but there were no significant difference among group 2~4 (P>0.05 all) .Conclusion:The thrombolytic recanalization rate is highest ,and the 30d all-cause mortality and incidence rate of adverse reactions are lowest for thrombolysis within 3 h from onset .

Objective To investigate the changes of coagulation and fibrinolysis systems in patients with solid cancer. Determine the mechanism of thrombosis formation in solid cancer and the the metastasis mechanism of solid cancer.Methods To measure plasma concentrations of TF,TFPI,t-PA,u-PA,PAI-1 by ELISA and test protein C activity(PC:A)by chromogenic substrate assay.Results Plasma concentrations of TF,TFPI,u-PA,PAI-1 were all higher in solid cancer patients than normal control and higher in metasta- sized team than non-metastasized team.In the dead team,u-PA and PAI-1 were higher while TFPI was low- er. t-PA was higher in cancer patients combined with venous thromboembolism while protein C activity was lower.Conclusion Disorders of coagulation and fibrinolysis system are related to the thrombosis formation in solid cancer.Coagulation and fibrinolysis factors take part in the metastasis progress of solid cancer. High concentration of u-PA and PAI-1 or low concentration of TFPI are considered to be related with poor outcome.

BACKGROUND: It has been demonstrated that electromagnetic field (EMF) can adjust proliferation and differentiation of bone marrow mesenchymal stem cells, but the specific mechanism is not clear. OBJECTIVE: To investigate the effects of EMF-activated ERK1/2 pathway on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells.METHODS: The 3rd passage of rat bone marrow mesenchymal stem cells were received EMF treatment (15 Hz, 1 mT, sine wave), 20 μmol/L PD98059 + EMF treatment, or only PD98059 treatment. Simultaneously, a normal control group was established. Western blotting was applied to detect the activation of ERK signal pathway after EMF exposure. MTT assay was used to determine the activation of proliferation of cells. And alkaline phosphatase (ALP) activity in cells was detected by an ALP kit. RESULTS AND CONCLUSION: The ERK1/2 phosphorylation, proliferation and ALP activity of rat bone marrow mesenchymal stem cells were remarkably increased after exposure to EMF (P < 0.01). PD98059 could effectively block the increasing of ERK1/2 phosphorylation and cell proliferation (P < 0.01), but elevate ALP activity in a certain level (P < 0.01). EMF stimulation can fast activate ERK1/2 signal pathway and then promote the proliferation of rat bone marrow mesenchymal stem cells, however, ERK1/2 signal pathway activation has a less effect on osteogenic differentiation of bone marrow mesenchymal stem cells.

Objective To analyze the core authors, and their organizations, geographical regional distribution of Chinese Journal of Orthopaedics. Methods The core authors, coauthors and their organizations whose articles were published in Chinese Journal of Orthopaedics between 1995 and 2004 were quantitatively analyzed using literature search through CMCI, which programmed by the People's Liberation Army Medical Library and their geographical regions distribution, the organizations of whom had high quantitative publication were determined using literature metrology methods. Results There were 2660 articles published in Chinese Journal of Orthopaedics between 1995 and 2004. All of 1123 authors of 2660 articles had only one article published which accounted for 69.67% of total first authors on the Chinese Journal of Orthopaedics in ten years. There were 2385 articles with one or more coauthors and the cooperative rate was 89.66%, and cooperative degree was 4.11 in the period of time. There were 275 articles with single author, which accounted for 10.34% of total articles. The articles written by the authors from military hospitals, university affiliated hospitals and provinces-level hospitals were 646(24.28%), 1091(41.01%) and 706(26.54%), respectively during the period. There were 218 core authors with 959(36.05%) articles published and there were 20 high-quantities organizations with 1007(37.86%) articles published in this study. The number one region of published articles was Beijing with 667(25.08%) articles. There were 2443 (91.84%) articles pressed from the organizations of affiliated hospitals of universities, provinces-level hospitals and army hospitals. Among them, Tianjin hospital (101 articles), Beijing Jishuitan hospital (100 articles) and affiliated Xijing hospital of the 4th military medical mniversity (79 articles) occupied 10.53% of the pressed papers and they were in the first three positions. Conclusion Authors of Chinese Journal of Orthopaedics have a wide distribution and highly cooperative rate. There were a group of active and talented core authors who has a great influence on the journal.

Objective To evaluate the efficacy and adverse effects of docetaxel combined with 5-fluorouracil in treating patients with advanced gastrice cancer.Methods 130 patients with advanced gastric cancer were randomly divided into observation group and control group,observation group treated with docetaxel combined with 5-fluorouracil,the control group treated with 5-fluorouracil and cisplatin,the clinical efficacy after the treatment was observed.Results The clinical efficiency rate of the observation group was significantly higher than that of the control group,the rate of leukopenia,thrombocytopenia and gastrointestinal reactions of the observation group was significantly lower than the control group,the quality of life scores of the two groups after treatment were all improved,but that of the observation group was significantly higher than the control group,the difference was statistically significant(P ＜ 0.05).Conclusion Docetaxel combined with 5-fluorouracil was effective in treating advanced gastric cancer.It worth further clinical research and application.

Gene expression exhibits temporal and spatial patterns to response environmental changes and growth cycle. Gene expression is under strict control at different levels among which control at transcription level is the predominant mode, especially in prokaryotes. In this review, we summarized the new developments of methods used in transcriptional studies, including modifications and improvements of the classic methods, such as gel-shift assay, DNA foot printing, and in vivo reporter system. In addition, we introduced examples to apply new methods, such as surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) to characterize protein-DNA, ligand-protein, and ligand-protein-DNA interactions. The collection of these methods and their application could guide and accelerate relevant studies.
Calorimetry ; DNA Footprinting ; Gene Expression ; Ligands ; Proteins ; Surface Plasmon Resonance ; Transcription, Genetic