Objective The invasion and metastasis of tumor cells depend on the growth of new blood vessels, and tumor neovascularization was regulated by a lots of factors. This study aimed to investigate the expression of hypoxia-inducible factor-1α (HIF -1α) and vascular endothelial growth factor (VECF) in retinoblastoma and explore the correlation of HIF-1α and VEGF with tumor invasion and metastasis and analyze their relationship with clinicopathology characters and determine their effect on angiogenesis. Methods Forty-six retinoblastoma specimens with different clinical stages were collected in Affiliated Second Hospital of Nanchang University. The specimens received neither radiotherapy nor chemotherapy. The retinal tissue near the tumor was as control. Immunohistochemistry was used to detect the expression of HIF-1α protein and VEGF protein in retinoblastoma. The relationship between expression of HIF-1α and VEGF and stage of tumor was analyzed. Results The HIF-1α and VEGF were highly expressed in the ischemia or necrosis area of retinoblastoma. Expression of HIF-1α was mainly in cell nuclear and partly in cytoplasm, and VEGF was mainly expressed in cytoplasm of tumor cells. The expression of HIF-1α and VEGF was gradually increased with the rising of tumor stage (P ＜ 0. 05) and showed significant correlation between expression of HIF-1α and VEGF with tumor stage(r_s =0. 943, P ＜0. 01). HIF-1α exepression was also possitively related to VEGF level in retinoblastoma (r, =0. 946, P ＜ 0. 01). Conclusion HIF-1α and VEGF were over-expressed in retinoblastoma cells, and the expressions were related to clinical staging, invasion and metastasis of tumor cells. The expression of HIF-1α and VEGF was one of the predictors for the biological behaviors of retinoblastoma. HIF-1α and VEGF may play an important role in angiogenesis and tumor progression in retinoblastoma.

Objective:To study expression of complement C1q tumor necrosis factor related protein 3 (CTRP‐3 ) in obese patients and its significance .Methods :A total of 402 obese patients were enrolled as obesity group and 405 normal people undergoing physical examination were regarded as normal control group . The correlation among CTRP‐3 level and related indexes were analyzed ,and multi-factor regression analysis was used to study independent risk factors for obesity .Results:Compared with normal control group ,there were significant rise in body mass index (BMI) ,homeostasis model -insulin resistance index (HOMA-IR) ,levels of systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,total cholesterol (TC) ,low density lipoprotein cholesterol (LDL‐C) ,triglyceride (TG) ,fasting blood glucose (FBG) ,insulin ,glycosylated hemoglobin (HbA1c) and high sensitive C reactive protein (hsCRP) ,and significant reductions in levels of high density lipoprotein cholesterol (HDL‐C) ,adiponectin (APN) ,leptin and CTRP‐3 in obesity group , P<0.05 or <0.01 . Spearman correlation analysis indicated that after age and gender correction ,CTRP‐3 level was significant inversely correlated with BMI (r= -0.221) ,SBP (r= -0.031) ,DBP (r= -0.043) ,TC (r= -0.147) , LDL‐C (r= -0.051) ,TG (r= -0.743) ,FBG (r= -0.238) ,insulin (r= -0.053) ,HOMA -IR (r= -0.281) , HbA1c (r= -0.741) and hsCRP levels (r= -0.216) ,P<0.05 or <0.01 ,and significant positively correlated with lev‐els of HDL‐C (r=0.351) ,APN (r= 0.852) and leptin (r=0.641) ,P<0.05 all .Multi-factor regression analysis indi‐cated that after correcting other influencing factors ,compared with high tertile CTRP‐3 group ,there were significant rise in OR value in middle tertile CTRP‐3 group (OR=6.47 ,95% CI 3.58 -12.18) and low tertile CTRP‐3 group (OR=12.39 ,95% CI 3.58-29.15) , P<0.01 all .Conclusion:CTRP‐3 level is significantly correlated with obesity -related fac‐tors ,and it′s an independent risk factor for obesity .

Objective To investigate clinical effect and mechanism of Yudanrongxin pills in the treatment of patients with acute myocardial infarction ( AMI) .Methods 86 cases of AMI in our hospital from February 2014 to February 2015 were selected and divided into the observation group and the control group, with 43 cases in each group.The patients in the control group were treated with conventional therapy, while patients in observation group were treated with Yudanrongxin pills on the basis of the control group.The related inflammation factors, indicators of myocardial injury and heart function index were compared between two groups before and after treatment.Results Compared with before treatment, in both two groups after treatment, the serum inflammatory factors including high-sensitivity C-reactive protein(hs-CRP), IL-6,soluble vascular cellular adhesion molecule-1 (sVCAM-1), TNF-αand P-selectin decreased, the myocardial injury criterion including creatine kinase-MB (CK-MB), lactate dehydrogenase (LD), myocardial troponin I (cTnI) and myoglobin (Mb) decreased,the cardiac function indexes of left ventricular end systolic volume (LVESV) and left ventricular end diastolic volume (LVEDV) decreased and left ventricular ejection fraction (LVEF) increased (P<0.05).Compared with control group, the hs-CRP,IL-6,sVCAM-1,TNF-α,P-selectin,CK-MB,LD,cTnI and Mb in observation group were lower(P<0.05).The degree of improvement at cardiac function was better than that in control group (P<0.05).Conclusion Yudanrongxin pills could better improve cardiac function in treatment with AMI, its role was relative to inhibition of inflammatory factors and myocardial protection against injury.

Objective:To study expression of complement C1q tumor necrosis factor-related protein 3 (CTRP-3)in patients with hypertension and its significance.Methods:A total of 562 patients with hypertension were enrolled as hypertension group,and 570 normal subjects undergoing physical examination were regarded as normal control group. General data,plasma levels of adiponectin (APN),leptin and CTRP-3 etc. were measured and compared be- tween two groups. Correlation among CTRP-3 level and related factors of hypertension were analyzed. Results:Compared with normal control group,there were significant reductions in plasma levels of APN [(12.1±0.4)μg/ml vs. (7.3±0.5)μg/ml],leptin [(10.1±0.4)ng/ml vs. (6.2±0.8)ng/ml]and CTRP-3 [(429±15)ng/ml vs. (314±13)ng/ml]in hypertension group,P<0.05 all. Spearman correlation analysis indicated that after correcting age and gender,plasma CTRP-3 level was significant inversely correlated with body mass index,systolic blood pres- sure,diastolic blood pressure,waist circumference,waist hip ratio,levels of total cholesterol,low density lipopro- tein cholesterol (LDL-C),triglyceride,fasting blood glucose,insulin,homeostasis model-insulin resistance index (HOMA-IR),glycosylated hemoglobin,high sensitivity C reactive protein (hsCRP)(r=-0.852~-0.011,P<0.05 or <0.01),and significant positively correlated with levels of high density lipoprotein cholesterol (HDL-C), APN and leptin (r=0.654~0.962,P<0.05 all). Multi-factor regression analysis indicated that compared with high tertile CTRP-3 group,the OR=14.17 (95%CI:3.62~28.34),P=0.001 in low tertile CTRP-3 group,sug- gesting that low plasma CTRP-3 level was independent risk factor for hypertension.Conclusion:Complement C1q tumor necrosis factor-related protein 3 level significantly correlated with hypertension,and it's an independent risk factor for hypertension.

AIM: Test the character of Electrooculogram (EOG) in normal subjects so as to obtain reference values.METHODS: By using Vision Monitor visual evoked response imaging system, the EOG was recorded on 60 normal subjects (73 eyes).RESULTS: EOG under the condition of normal pupil was recorded in normal subjects according to ISCVE standard. The dark trough potential was (701.8±265.1)μV, the light peak potential was (1255.0±447.7)μV, the Arden ratio (light peak /dark trough ratio)was 180%±21%.CONCLUSION: Our study reflected the spatial characteristics of electrooculogram in normal subjects,provided reliable normal reference values for clinical research.

Adult ; Female ; Humans ; Rectal Neoplasms ; diagnosis ; pathology ; Rectum ; pathology ; Sarcoma, Myeloid ; diagnosis ; pathology

Background Retinal development continues during the early postnatal period in mammals.Correct arrangement of layers and precise location of various cells in the retina are vital for forming normal visual function during critical period plasticity.Spectral-domain optical coherence tomography(SD-OCT)provides highquality in vivo retinal imaging and the possibility to measure retinal thickness longitudinally. Objective The present study was to investigate the changes of retinal thickness during critical period plasticity in rats. Methods In vivo consecutive scanning of retinal image was performed in 10 SPF Sprague-Dawley rats at postnatal day 14(P14),P18,P21,P24 and P42 with SD-OCT,and retinal histopathological examination was used to detect retinal morphologic changes at the same postnatal ages in 20 matched rats.The whole retinal thickness,the thickness from inner limiting membrane(ILM)to inner plexiform layer(IPL),the thickness of inner nuclear layer(INL)and the thickness from outer nuclear layer(ONL)to retinal pigment epithelium(RPE)were measured using Cirrus HD-OCT system and HMIAS-2000 Imaging System in retinal sections.The measurement parameters by Cirrus HD-OCT and those by hematoxylin-eosin staining were compared.The use of animals followed the Statement of National Institute of Health (USA). Results In vivo high-resolution images of rat retinas with SD-OCT compared well with histology,which enabled quantitative comparison of the SD-OCT and histological data during critical period plasticity in rats.From P14 to P42,the retinal thickness gradually decreased with the increase of rat ages(F=15.425,P=0.000),and so were the thickness from ILM to IPL,the thickness of INL and the thickness from ONL to RPE(F=3.973,P=0.007;F=17.529,P=0.000;F=7.038,P=0.000).The retinal thickness,thickness of INL.thickness from ONL to RPE measured by Cirrus HD-OCT were significantly correlated with those measured by retinal sections among P14,P18,P21,P24 and P42 rats(r=0.794,P=0.000;r=0.784,P=0.000;r=0.681,P=0.000). Conclusion SD-OCT is a demonstratably valuable technology to study the structure of retinas in rats.The retinal thickness is shown to reduce in thickness throughout the development of the retina during critical period plasticity due to the decrease in thickness of INL and the distance from the ONL to RPE,as illustrated by OCT scanning.

Objective To observe the characteristic changes in cardiac function and to raise diagnostic accuracy and sensibility.Methods 39 silent myocardial ischemia and 58 angina and 46 age-matched healthy subjects and 62 obsolete myocardial infarction underwent myocardial tomography imaging and gated ventricular imaging.Covariance analysis was used to compare difference between SPECT imaging and parameters of function.The age was tested as covariate factor,then SNK-Q test and multiple comparison were carried on.Results The silent myocardial ischemia and angina showed reversible radioactive defect or fixed defect.Most of myocardial SMI showed single peak exempt TPFR.EF,PFR and PER showed remarkably statistical difference (P<0.01).The multiple comparison of PFR and PER was increased according by OMI,AN,SMI and negative control group (P<0.05).Conclusions The myocardial damage and PFR,PER in SMI is lower than that in AN group,which have no remarkably influence on the parameters of diastolic and retractile function in left ventricular.

Objective To explore the relationship between blood lipids level with creatinine clearance rate(Ccr)in patients with heart failure(HF). Methods A total of 955 patients who were diagnosed with heart failure(cardiac function NYHAⅡ~Ⅳclassification)upon discharge from the Department of Cardiology of the Second Hospital of Tianjin Medical University, between January 2010 to June 2013 were enrolled as HF group. Healthy adults (n=200) with normal cardiac function which approximately matched basic condition with HF group were selected as control group. The HF group was fur?ther divided intoⅡ,Ⅲ,Ⅳclassification according to their cardiac function(NYHA classification). HF group was also divid?ed into normal renal function group, mild renal injury group and moderate-severe renal injury group based on their Ccr. Ef?fect of gender and lipid parameters were also compared. Binary logistic regression was used to analyze factors influencing renal function in patients with HF. Results Compared with people in the control group, the levels of triacylglycerol(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-c)and non high density lipoprotein cholesterol(non-HDL-c)in patients of HF group were increased while Ccr and high density lipoprotein cholesterol(HDL-c)were decreased. Ccr and lipids were obviously decreased in patients with HF of Ⅳclassification. TG and HDL-c were decreased in moderate-severe renal injury group. Females had a higher lipid levels than males in HF group(P<0.05 or P<0.01). Advanced age, coronary heart disease and hypertension were all risk factors for renal impairment in patients with HF by binary logistic re?gression. On the other hand, weight gain and HDL-c were the protection factors for renal function in HF patients. Conclu?sion Dyslipidemias may lead to renal insufficiency in patients with HF. It was important to control lipids and improve re?nal function in patients with HF.

Background and purpose: It has been demonstrated that cyclooxygenase-2 (COX-2) is over-expressed in some subtypes of non-Hodgkin’s lymphoma (NHL), and COX-2 correlates with the expression of P-glycoprotein and Bcl-2, which may contribute to chemotherapy-resistance in NHL. The purpose of this study was to investigate the expression of COX-2 in B-cell lymphoma cell lines and the potential mechanisms of celecoxib, a selective COX-2 inhibitor, to sensitize lymphoma cell lines to epirubicin. Methods: Quantitative fluorescent real-time poly-chain-reaction (qRT-PCR) and Western blot were employed to determine the expression of COX-2 in Raji, Jeko-1 and Namalwa cell lines, as well as in peripheral blood B cells from normal controls. Cell lines were treated with celecoxib at gradient concentrations, followed by the detection of cell viabilities by cell counting kit-8 (CCK-8).Meanwhile, the changes in expression of MDR-1 mRNA and Bcl-2 mRNA before and after celecoxib treatment were determined by qRT-PCR. Raji cells were treated with epirubicin alone or in combination with gradient concentrations of celecoxib for 72 h, then CCK-8 was used to analyze whether celecoxib sensitize Raji cells to epirubicin. Results:Neither lymphoma cell lines nor normal B cells expressed detectable COX-2 in this study. Celecoxib inhibited the proliferation of the 3 lymphoma cell lines, and the mRNA expressions of MDR-1 and Bcl-2 were decreased by celecoxib in a concentration-dependent manner, except for that MDR-1 was undetectable in Jeko-1 cells. In addition, celecoxib sensitized Raji cells to epirubicin, indicating a synergistic anti-tumor effect between the two agents. Conclusion:Selective COX-2 inhibitor celecoxib down-regulates the expressions of MDR-1 mRNA and Bcl-2 mRNA in B-cell-originated lymphoma cell lines, and sensitizes Raji cells to epirubicin.