Objective To describe the diagnosis and preoperation evaluation of pancreatic carcinoma with spiral CT three-phase contrastenhanced scan and post-processing.Methods Three-phase helical CT enhanced scan and post-processing images of 41 patients with pancreatic carcinoma were retrospectively analyzed,including the location,size,characteristic of tumor and peripancreatic blood vessels,the results were compared with that of surgery.Results Low degree of enhancement of pancreatic carcinoma in pancreatic phase was showed compared with parenchymal phase.There was significant difference in density between focus and normal pancreas,the lesions were showed clearly.In comparison with surgical results,the diagnostic accuracy of CT was 80.7% and the accurate rate in evaluating the metastasis was 75.8%.Conclusion Three-phase helical CT enhanced scan and postprocessing are of great value in diagnosis of pancreatic carcinoma and it is helpful in choosing the treatment program preoperatively and assessing the respectability of tumors.

OBJECTIVE:To establish an HPLC method for the content determination of oxaliplatin in Oxaliplatin liposome. METHODS:The separation was performed on Hypersil C18(250 mm?4.6 mm,5 ?m) column with methanol-water(5 ∶ 95,V/V) as mobile phase at flow rate of 1.0 mL?min-1. The detection wavelength was set at 250 nm and column temperature was 30 ℃. RESULTS:The linear range of oxaliplatin was 12.2～122.0 ?g.mL-1(n=5,r=0.999 9) with an average recovery of 98.58%(RSD=1.83%). The limit of detection(LOD) was 97.6 ng?mL-1. CONCLUSION:The method is simple,sensitive and accurate for the content determination of oxaliplatin in Oxaliplatin liposome.

Objective:To compare the effect of laparoscopic uterine isthmus cerclage and transvaginal uterine isthmus cerclage in the treatment of cervical incompetence at non pregnant condition.Methods:A total of 63 patients with cervical incompetence from May 2013 to May 2015 in our hospital were enrolled in the retrospective analysis,all the enrolled patients had naturally conceived single birth with complete data after laparoscopic uterine isthmus cerclage or transvaginal uterine isthmus cerclage and were divided into two groups according to two different surgical methods for uterine isthmus cerclage to compare the clinical effect.30 patients treated with laparoscopic uterine isthmus cerclage were,in the research group and the other 33 patients treated with transvaginal uterine isthmus cerclage were in the control group.Results:The treatment success rate of research group (96.67％) was significantly higher than that of the control group(51.52％) (P ＜ 0.05).The research group had got a longer average pregnancy period than control group (P ＜ 0.05).Postoperative abortion rate (3.33％) and preterm birth rate(10.00％) of research group were obviously lower than the control group (48.48％,30.30％)(P＜0.05).The term infant rate of research group (86.67％) was higher than control group (21.21％) (P ＜0.05).The operation time((37.27 ± 1.93 min) and hospital stay(5.17 ±0.38 d) of the research group were less than the control group(P＜0.05).The bleeding amount in surgery of research group(13.13 ±1.57ml) was significantly lower than the control group(31.61 ± 1.87 ml) (P ＜ 0.05).The complication rate of observation group was 0,and the control group was 18.18％.The difference was significant(P ＜ 0.05).Conclusions:Laparoscopic uterine isthmus cerclage in treatment of cervical imcompetence at non pregnant condition has better clinical effect than transvaginal uterine isthmus cerclage.It has higher security and feasibility.It is worth clinically promoting.

Objective To investigate the effects of electroacupuncture of different stimulation waveforms on the 5-hydroxytryptamine (5-HT), glutamic acid (Glu) and gamma-aminobutyric acid (CABA) contents of the hypothalamus in rats with para-chlorophenylalanine (PCPA)-induced insomnia and explore the difference in the effects and the mechanism of its action. Methods Fifty SD rats were randomly allocated to a normal group of 10 rats, a model group of 10 rats and an electroacupuncture group of 30 rats. The electroacupuncture group was then randomly divided to electroacupuncture groups 1, 2 and 3, 10 rats each. A rat model of insomnia was made using PCPA in all groups of rats except the normal group. All the electroacupuncture groups received electroacupuncture treatment. Of them, electroacupuncture group 1 received sparse wave treatment; electroacupuncture group 2, dense wave treatment;electroacupuncture group 3, sparse-dense wave treatment. After 6 days of treatment, every group of rats was sacrificed by cervical dislocation. The 5-HT, Glu and CABA contents of the hypothalamus were measured by enzyme-linked immunosorbent assay. Results The 5-HT content of the brain decreased significantly in the model group of rats compared with the blank group (P<0.01) and increased significantly in electroacupuncture group 3 of rats compared with the model group (P<0.01). There was a statistically significant difference in brain 5-HT content between electroacupuncture group 2 or 3 of rats and electroacupuncture group 1 (P<0.05,P<0.01) and between electroacupuncture groups 3 and 2 of rats (P<0.01). The Glu and CABA contents of the cerebral cortex and the ratio of Glu/CABA increased significantly in the model group of rats compared with the blank group (both P<0.01) and decreased significantly in electroacupuncture group 3 of rats compared with the model group (both P<0.01). There were statistically significant differences in the Glu and CABA contents of the cerebral cortex between electroacupuncture group 2 or 3 of rats and electroacupuncture group 1 ((P<0.05,P<0.01) and between electroacupuncture groups 3 and 2 of rats (P<0.01). Conclusions Electroacupuncture can regulate hypothalamic monoamine neurotransmitter 5-HT content and amino acid neurotransmitters Glu and CABA contents and reduce intracerebral amino acid toxicity to produce a therapeutic effect on insomnia in insomnia rats. The therapeutic effect of sparse wave electroacupuncture is superior to that of dense wave or sparse-dense wave electroacupuncture.

Objective To explore the clinical value of 18F-FDG PET/CT in the diagnosis of cardiac neoplasm.Methods Between January 2007 and December 2011,18F-FDG PET/CT was performed in 8649 patients in our hospital,and 14 (0.16％ ; 11 males,3 females,average age 46.9 (35-68) years) patients were diagnosed with cardiac neoplasm.18F-FDG PET/CT data of those 14 patients were retrospectively analyzed.Final diagnoses were confirmed by either histopathology (n =5) or follow-up (n =9).Results Accurate tumor localization and diagnoses were made by 18 F-FDG PET/CT in all 14 patients.Among them,13 were malignant (2(14.3％,2/14) primary and 11(78.6％,11/14) metastatic tumors (8 HCC,2 lung cancer,1 esophageal cancer) ; SUVmax 3.2-10.7) and 1 (7.1％,1/14) was benign (myxoma).Conclusion 18F-FDG PET/CT is useful for the detection and staging of primary malignant and metastatic cardiac neoplasms.

Background Microvessels are composed of two interacting cell types: endothelial cells and pericytes. Over the past decades, studies of corneal angiogenesis have concentrated mainly on endothelial cells, while interest in pericytes has lagged behind. Objective The present study aimed to investigate the recruitment of vascular endothelial cells and pericytes in rat corneas after alkali burn. Methods Corneal alkali burn models were established in the right eyes of 36 adult SPF SD rats by putting 4 mm medicators containing a 1% 1 mol/L NaOH solution at the central corneas for 30 seconds, and 3 matched normal rats were used as controls. Corneas were excised 1,2,3,5,7 and 10 days after surgery. Frozen sections that parallel with the corneoscleral limbus were constructed. Double immunofluorescence staining was used to observe the dynamic expression of CD31 and α-smooth muscle actin (α-SMA) in corneal tissue for the evaluation of the number of endothelial cells and pericytes. The pericyte coverage index (PCI) was calculated to quantify the recruitment of pericytes to neovascular sites. The use of experimental animals followed the Statement of Association for Research in Vision and Ophthalmology. Results CD31 was expressed in the superficial stromal layer of the cornea on the 1 st day, showing the presence of red fluoresence. The positive cell number for CD31 was gradually increased with the passage of time and proceeded into the deep stromal layer from days 2 through 5 but decreased after that. However,α-SMA was positively expressed on the 2nd day in the cornea after alkali burn with the presence of green fluorescence. The positive cell number for α-SMA was less than those of CD31 throughout the experimental period. The PCI was 0, 16.07%, 11.95%, 43.84%, 73.97% and 86. 21% , respectively, 1,2,3,5,7 and 10 days after surgery. Conclusion Pericytes recruitment to corneal new vessels may play a key role in the stabilization and maturation of angiogeneis.

Objective To establish a desirable quantitative assay system to evaluate the correlation between surgical manipulation and micrometastasis in primary esophageal cancer patients.Methods 118 pe- ripheral blood samples from 40 esophageal cancer patients undergoing radical resection were collected before surgery(B-1),immediately after surgery(B0)and at the third day postoperatively(B+3).Based on real-time quantitative reverse transcription-PCR,carcinoembryonic antigen(CEA)mRNA gene was used in the detec- tion.12 patients with benign tumor and 13 healthy volunteers were collected as negative control groups.Re- sults The median of CEA mRNA copies at B-1,B0 and B+3 were 1592,13 314 and 6221 copies/ml blood, respectively.CEA mRNA expression levels were found to be significantly higher at both B0 and B+3 than that of B-1(P=0.0001 and 0.0209,respectively).No significant difference was found between B0 and B+3(P= 0.4396).Conclusion Surgical manipulation on esophageal cancer patients increases the probability of mi- crometastasis.Therefore,adjuvant therapy is needed during perioperative stage.

Objective To investigate the effects and mechanisms of selective angiotensinⅡtype 1 receptor antagonist ZD7155 on the inhibition of pancreatic cancer in vitro.Methods MTT assays were used to determine the inhibition of pancreatic cancer cell line PaTu8988s by ZD7155 in different concen- trations and at different time.PaTu8988s cell cycle and cell apoptosis were detected by flow cytometry. Transmission electron microscope was used to investigate the apoptosis of PaTu8988s before and after the incubation with ZD7155 under different concentrations.PaTu8988s cell morphology was observed be- fore and after the incubation with ZD7155.Results MTT showed that the increase of inhibition of pan- creatic cancer cell by ZD7155 was in agreement with the increase of the concentrations of ZD7155 and the time of the incubation with ZD7155.The inhibition rates of PaTu8988s cells were 9%,18%,30%, 51%,60% and 78% by ZD7155 with the concentrations of 5?10~(-11),5?10~(-10),5?10~(-9),5?10~(-8),5?10~(-7) and 5?10~(-6) mol/L,respectively.The inhibition rates of PaTu8988s cells were 15%,25%, 36%,51%,67% and 85% by ZD7155 with the same concentration(5.0?10~(-8) mol/L)at 12,24,36, 48,60 and 72 hours,respectively.ZD7155 could also inhibit PaTu8988s cell cycle significantly and was dose-dependent.Cell electron microscopy showed that there were chromatin margination and apoptotic body in the cell nucleus when the cells were incubated with ZD7155,and these changes were increase with the concentrations of ZD7155.The morphology of PaTu8988s cell didn't have any change after in- cubation with ZD7155.Conclusions ZD7155 can inhibit the growth of pancreatic cancer cells in vitro by suppressing the S-phase of cell cycle and induce cell apoptosis without visible cell toxic effects.

Objective To observe the effect of coronary artery disease on QT dispersion (QTd). Methods The QTd and QTcd in 245 patients underwent percutaneous coronary angiography (CAG) were analyzed. It was evaluated The relations between the QTd and the degree of coronary stenosis and the number of disease vessels. Results The QTd and QTcd were significantly prolonged in patients with coronary artery stenosis than those in patients with normal coronary artery(P0.05). Conclusion There is no relation between the number of disease coronary artery and the QTd.

OBJECTIVE: To construct nasopharyngeal carcinoma CNE-2 cell lines expressing stable fusion suicide gene CD/UPRT. UL49. METHOD: The plasmids of pcDNA3.1 (-)E6. BARF1p. CD/UPRT. UL49 was transfected into CNE-2 cells through lipofectamine, and the transfected CNE-2 cells were selected by G418 and prodrugs for getting the cells expressing fusion CD/UPRT. UL49 gene. The protein produced by the suicide gene was tested by Western-blotting in CNE-2 cells. RESULT: Suicide genes were expressed stably in CNE-2 cells. CONCLUSION We constructed nasopharyngeal carcinoma cell lines CNE-2 expressing stable suicide gene through lipofectamine.
Artificial Gene Fusion ; methods ; Carcinoma ; Cell Line, Tumor ; Genes, Transgenic, Suicide ; Humans ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; genetics