1.Lactate and tumor
Journal of International Oncology 2012;39(2):111-113
Lactate is considered a dead-end product of glycolysis.Its generation and accumulation promotes tumor growth and metastasis,and it is related to the poor prognosis of tumor.But recent studies have found,tumor cells can also uptake and utilize lactate.With the recent advances in tumor metabolism and gene therapy,lactate is a potential therapeutic target in tumors.
2.Role of NF-?B p50 protein in pathologic responses of experimental acute pancreatitis in mice
Chinese Journal of Current Advances in General Surgery 2004;0(05):-
Objective:To observe the roles of p50 protein in pathologic responses of cerulein(CR)pancreatitis in mice deficient in p50 protein of NF-?B.Methods:Pancreatitis was induced by i.p.injection of 50?g/kg CR.The express and compensation of p65,p50 and cRel proteins of NF-?B,NF-?B activity were measured using electromobility shift assay;parameters of pancreatitis,eg,amylase,lipase and trypsin were evaluated.The apoptosis,necrosis and caspase-3 activity were observed.Results:p50 knockout(KO)mice had no p50 protein expression and increased p65 and cRel proteins expression.p50 KO mice's NF-?B activity decreased and apoptosis increased in pancreatitis compared with wild type(WT)mice.There was no caspase-3 activition in both mice before and after pancreatitis.All KO mice had an increased necrosis and amylase,lipase,trypsin activity compared with WT mice in pancreatitis.Conclusions:The results indicated that NF-?B p50 genetic deletion might attenuate cerulean induced pancreatitis.
3.Treatment of supra-condylar fractures of the humerus with a unilateral external fixator
Gang ZHAO ; Lei HUANG ; Manyi WANG
Chinese Journal of Orthopaedic Trauma 2009;11(8):710-713
Objective To evaluate the treatment of supra-condylar fractures of the humerus (12-B1.2 by AO classification) with a unilateral external fixator. Methods From 2003 to 2006,28 cases of supra-condylar fractures of the humerus were treated with a unilateral external fixator. They were 15 males and 13 females. Their average age was 43 (24 to 58) years old. They were all closed fractures and 2 cases complicated with radial nerve injury. Results All were followed up for an average of 28 (12 to 54) months. All fractures united with an average healing time of 4.5 months. The 2 cases of radial nerve injury recovered completely. Five cases had infection of pin holes. The patients had an average elbow score (Money and Bryan system) of 97 and an average shoulder score (Constant and Murley system) of 98. Conclusion The unilateral external fixator is appropriate for supra-condylar fractures of the humerus ( 12-B1.2 by AO classification),because it provides not only effective fixation but also early functional rehabilitation.
4.The analysis of survival factors of patients with unresectable pancreatic cancer aged 70 years and over
Yingying HUANG ; Yunbo ZHAO ; Gang CHENG
Chinese Journal of Geriatrics 2010;29(6):483-485
Objective To explore the effect of related factors on survival of patients with unresectable pancreatic cancer aged 70 years and over. Methods Fifty-seven patients with unresectable locally advanced or metastatic pancreatic cancer aged 70 years and over were enrolled.Their survival time were analyzed with SPSS 13.0 by taking account of gender, age, smoking history,alcohol history, pancreatic disease history, diabetes mellitus history, Eastern Collaborative Oncology Group (ECOG) scoring, chemotherapy, radiotherapy, CEA and CA199 levels. Results Gender,ECOG scoring, chemotherapy and radiotherapy had relationship with overall survival. The median survival time was 8.9 months and one-year survival rate was 28.1%. The median survival was (10.7±5.4) months in male group and (5.5±2.3) months in female group (P=0.000). The median survival was(10.1±5.8) months in patients with ECOG 0~1 group and(7.3±3.8)months in patients with ECOG 2 group (P=0.040). The median survival was(7.76±5.27) months in nochemotherapy group and(11.5±5.0)months in chemotherapy group (P=0.038). The median survival was(8.87±5.36)months in no radiotherapy group and (13.7±3.8) months in radiotherapy group (P=0.048). Conclusions The patients who have better ECOG performance status and receive chemotherapy or radiotherapy show better survival.
5.The requirement of ethics protocol of clinical admission restrictions on the med-tech usage
Shuangmiao WANG ; Gang HUANG ; Bin ZHAO
Chinese Medical Ethics 1996;0(01):-
This article explores ethic issues of clinical admission restrictions on the med-tech usage based on three aspects which are the expectation of standard management of the med-tech usage restriction,the absence of ethic standard of the clinical admission restrictions on the med-tech usage and the ethic protocol of clinical admission restrictions,in order to provide ethic support for the clinical admission restrictions on the med-tech usage.
6.Analysis of the Utilization of Opioids Analgesics for Cancer Pain in Inpatient Department of Our Hospital in 2014
Wenlian TU ; Hui HUANG ; Gang ZHAO
China Pharmacy 2015;26(35):4922-4924
OBJECTIVE:To provide reference for rational use of opioids analgesics. METHODS:1 882 prescriptions of opi-oids analgesics for cancer pain collected from our hospital in 2014 were analyzed by defined daily dose(DDD)and drug utilization index(DUI). RESULTS:The diseases of opioids analgesics prescriptions for cancer pain in our hospital in 2014 were mainly lung cancer,accounting for 42.19%. Commonly used opioids analgesics included Morphine sulfate sustained-release tablets,Oxycodone Hydrochloride sustained-release tablets and so on,and their DUI were all below 1.0. Top one drug in the list of amount and con-sumption sum was Morphine sulfate sustained-release tablets,and its main dosage form were tablets,injection and patches,in which tablets occupied the largest proportion,reaching 97.30%. CONCLUSIONS:The application of opioids analgesics for cancer pain in our hospital is basically rational in terms of drug types,dosage form and route of administration,but the dose of opioids an-algesics is small and their DUI is lower than 1;at the same time,there are a few irrational prescription.
7.Application of promoting blood circulation by removing blood stasis in peripherial blood vessel diseases Treatment
Gang ZHAO ; Qiu SUN ; Yanhong HUANG
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(06):-
Promoting blood circulation by removing blood stasis has a long history in peripherial blood vessel diseases treatment.Based on years of practice,the paper illustrated common methods and principles of treatment on pheripherial blood vessel diseases in promoting blood circulation by removing blood stasis treatment,which gives us new ideas on importance of peripherial blood vessel diseases treatment with promoting blood circulation by removing blood stasis.
8.Expression of human inducible nitric oxide synthase in fibroblast cell line V_(79) and effects of H_(4)Bon iNOS activity
Feng ZHENG ; Lan HUANG ; Xiaojing WU ; Gang ZHAO
Chinese Journal of Pathophysiology 2000;0(11):-
0.05, n= 6) NO - 2 production by pcDNA 3-iNOS cells without H 4B was higher (105 58?13 33) ( n= 6, P
9.Management of postt aumatic flexion contracture in knee joint using Ilizarov technique
Shengsong YANG ; Lei HUANG ; Xing TENG ; Gang ZHAO ; Manyi WANG
Chinese Journal of Orthopaedics 2012;32(5):462-466
ObjectiveTo evaluate the effectiveness of correction of posttraumatic flexion contracture of knee(FCK) using Ilizarov technique.MethodsSix male patients with posttraumatic FCK,aging from 9 to 43 years(mean,24.5 years),treated by using Ilizarov technique from January 2006 to December 2010,were retrospectively analyzed.The preoperative flexion deformity of knee ranged from 35° to 85° (mean,47.6°),and the range of motion ranged from 0° to 70° (mean,15.8°).Among them,five patients had old fracture of knee joint combined with equinus foot deformity (deformity ranging from 25° to 37°,with an average of 31.8°); one patient had femoral supracondylar fracture.We used circular external fixator to correct the FCK and equinus foot deformity gradually,the knee arthrodesis operation was performed in 4 patients because of the damaged hone structure and poor soft tissue condition.ResultsAll patients were followed up for 12-22 months(mean,18 months).The flexion of knee joint was recoverd from preoperative 47.67°±18.63° to postoperative 9.33°±3.50°.Plantar flexion of ankle joint in five equinus foot deformity was recovered from preoperative 31.80°±4.65° to postoperative 3.00°±4.47°.The kuee joint was arthrodesised successfully in 4 patients and the other 2 patients got 30° and 75° ROM.All the patients can walk with a cane,and satisfied with the result of treatment.Pin-site infection was found in 4 patients and was controlled within two weeks.ConclusionIlizarov technique is an effective treatment method for posttraumatic FCK.Knee arthrodesis should be performed when patients had damaged bone structure and poor soft tissue condition.
10.Effects of exenatide on the cell proliferation, invasion and apoptosis of human tongue squamous cell carcinoma SCC-25
Chao HUANG ; Feifei SHEN ; Gang LI ; Yue ZHAO
Tianjin Medical Journal 2015;43(5):461-464
Objective To detect the effects of exenatide on the related indicators of proliferation, invasion and apoptosis of cell line SCC-25. Methods SCC-25 cells were cultured in vitro. The expression level of glucagon like peptide 1 receptor (GLP-1R) was determined by Western blot assay in SCC-25 cells. SCC-25 cells were divided into four groups:control group and exenatide group (1,10 and 100 nmol/L). The ability of cell proliferation was detected using MTT assay after 24 h, 48 h and 72 h of culture. The ability of invasion was measured with Transwell assays. The expression levels of MMP-2, Caspase-3 and Phospho-p38 MAPK were measured by Western blot assay. Results GLP-1 receptor expression was found in SCC-25 cells. Compared with control group, the cell survival rate, invasion rate and the expression of MMP-2 were significantly decreased in SCC-25 group (P<0.05). The expression of Caspase-3 were significantly increased (P<0.05).Changes were in a concentration-dependent and time-dependent manner (P<0.05). The expression of Phospho-p38 MAPK was significantly increased at 24 h in 10 nmol/L exenatide group (P<0.05). Conclusion Exenatide can inhibit the cell proliferation and invasion, which may contribute the apoptosis by promoting expressions of Phospho-p38 MAPK and Caspase-3 of SCC-25 cells.