Objective To study the effects of the expression of hypoxia inducible factor-1 alpha (HIF-1?) induced by FasL on the invasiveness of rectal cancer cells. Methods With molecular cloning method, eukaryotic expression vectors of pcDNA3.1 FasL containing total length of FasL cDNA sequence were transfected into human rectal cancer cells HR-8348. The cell invasiveness was examined. A hypoxia model of HR-8348 was reproduced, and its invasiveness was assessed with transwell methodology. Expression of HIF-1? was assayed by Western bloting. Results The number of HR-8348 cells transfected with FasL penetrated transwell filter membrane was larger (12.930?2.434) than that of empty vector transfected cells (7.670?2.093) and control cells (8.133?1.959) with statistically significant differences (P0.05), but it was significantly higher in FasL positive HR-8348 cells than in FasL negative cells at 12h and 24h after hypoxia (P0.05). Conclusions FasL is an effective factor inducing HIF-1? expression, and FasL expression correlates positively with HIF-1? expression. Enhanced FasL expression in rectal cancer cells could induce higher expression of HIF-1?, enable the tumor cells to adapt to hypoxia environment, and enhance the invasiveness of tumor cells.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Humans ; Male ; Methotrexate ; administration & dosage ; Middle Aged ; Mutation ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; genetics

Objective To compare the clinical pathological characteristics in young and old patients with colorectal cancer (CRC) and investigate their relationship with prognosis. Methods A retrospective review was made in 68 CRC patients less than 35 years old and 322 CRC patients older than 65 treated in our hospital from July 1993 to July 2003. Their clinical manifestation, pathological feature, Dukes staging, misdiagnosis rate and results of following-up were compared. Results The main manifestation in young group was abdominal pain (69.1%), but in old group was hemafecia or mucous bloody stools (53.7%). The ratio of poorly differentiated neoplasm was obviously higher in young group (48.5%) than in old group (20.5%) (P

In this article,the professor LI respectively discuss the using of the ten merthods of tocolysis by eliminating disease in Synopsis of Golden Chamber from aspects of his own practical experience, original text and practical applications. The ten methods are: tonifying and harmonizing both yin and yang and keepping ying and weiqi in balance, eliminating blood stasis and dissolving abdominal mass and regulating qi and blood, warming yang and dispersing cold and warming uterus for tocolysis, moisturizing dryness by nourishing blood and clearing heat and relieving qi stagnation, inducing resuscitation and diuresis, excreting dampness and activating yang, tonifying blood and invigorating the spleen, clearing and dissipating dampness and heat, invigorating the spleen for eliminating dampness, warmming the middle warmer for tocolysis.

Objective To discuss the effect of interferon ?-2a on the radiosensitivity and cell cycle for nasopharyngeal carcinoma cell line CNE-I. Methods Interferon ?-2a was given by different concentration. Then the cells were radiated with X-ray (6?MV) and the cell survival rate was calculated. The change of cell cycle dynamics was measured with flow cytometry. Results The cell survival fraction was 0.62, 0.43 and 0.20 respectively after the interferon ?-2a was given by different concentration(0.5?106, 1.0?106 and 1.5?106?IU/L). The radiosensitization ratio was 1.16, 1.57 and 1.93 respectively. Compared with the control group, increasing cell percentage in G_1 and G_2+M phage and decreasing cell percentage in S phage were observed on 24 h after the interferon ?-2a(1?106?IU/L) was given(P

AIM:To investigate the effect of fluvastatin on the expression of serum and glucocorticoid inducible kinase 1 ( SGK1) and connective tissue growth factor ( CTGF) induced by aldosterone ( Ald) in rat mesangial cells (GMCs). METHODS:GMCs were divided into (1) control group; (2) aldosterone group with different concentrations and times; (3) Ald (10 -7 mol/L) + spironolactone (10 -9 mol/L) group; (4) Ald (10 -7 mol/L) + LY294002 (20 ?mol/L) group; (5) Ald (10-7mol/L) +SB203580 (20 mmol/L) group; (6) the group of Ald (10-7mol/L) + fluvas-tatin at different concentrations (10-7,10-6,10-5 mol/L); (7) Ald (10 -7mol/L) + fluvastatin (10 -5mol/L) + mevalonate (10 -4 mol/L) group; (8) Ald (10 -7 mol/L) + fluvastatin (10 -5 mol/L) + FPP (farnesyl pyrophosphate,10-4 mol/L) group; (9) Ald (10 -7mol/L) + fluvastatin (10 -5 mol/L) + GGPP (geranylgerany pyrophosphate,10 -4 mol/L) group. The protein levels of SGK1 and CTGF were determined by Western blotting. The levels of fibronection (FN),monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in the supernatants were determined by enzymelinked immunosorbant assay (ELISA). RESULTS:Aldosterone stimulated the protein expression of SGK1 and CTGF in cultured mesangial cells in a dose-dependent manner (P

Objective:To observe the clinical efficacy of warm needling moxibustion plus tuina in treating knee osteoarthritis (KOA) due to cold-dampness blocking collaterals.
Methods:Forty-eight KOA patients were randomized into 2 groups by their visiting sequence, 24 cases in each group. The treatment group was intervened by warm needling moxibustion plus tuina, while the control group was treated with acupuncture plus tuina. The two groups were both treated once a day, 30 min for each session, 10 d as a treatment course, totally for 3 treatment courses. The visual analogue scale (VAS) and Lysholm knee scoring scale (LKSS) were observed before and after the treatment to evaluate the clinical efficacy.
Results:After 3 treatment courses, the VAS and LKSS scores were significantly changed in the two groups (P<0.01), and the between-group differences were also statistically significant (P<0.01). The markedly-effective rate was 83.3% in the treatment group versus 66.7% in the control group, and the difference was statistically significant (P<0.05).
Conclusion:Warm needling moxibustion plus tuina can produce a more significant efficacy than acupuncture plus tuina in treating KOA due to cold-dampness blocking collaterals.

Objective Investigations regarding the status of pediatric emergency and relevant facilities distribution in our province represent a crucial prerequisite for further setting up emergency network and improving our first aid capabilities.Methods We had conducted relevant surveys on the medical institutions within our province,including lever 2 and lever 3 hospitals as well as maternal and child care service centers.Results The feedback from 151 institutions indicated that they had 4485 pediatric beds and treated 5 308 severe patients in 2010.Among all,38 hospitals had ventilators for children and 4 hospitals could perform pediatric blood purification treatment.However,no one had mastered the children ECMO technique.Conclusion There is an inadequacy of some emergency equipments and the development in emergency and first aid is uneven.There is a strong request to launch and develop the blood purification and bronchfiberscope techniques,in combination with improvements in the first aid capabilities and emergency network.

Objective:To obtain the normal values of the facial soft tissue measurements of the attractive Uygur females in Xinjiang by photogrammetric analysis,and to study the relationship between the facial feature of attractive Uygur females and the neoclassical canons. Methods:31 attractive Uygur women were selected, standardized and referenced frontal photographs of faces were taken,and 14 standard anthropometric marks were determined,then measurements were performed by photoshopCS software.Results:Intercanthal width was rather smaller than nose width and larger than eye fissure width;nose width occupied more than 1/4 of face width or 2/3 of mouth width.The chin occupied 2/3 of the entire mandibular height.There was positive correlation among many horizontal and vertical facial soft tissue measurements.Conclusion:The facial features of the attractive Uygur females in Xinjiang fit the classical facial canons to some extent. There is pertinence in attractive facial form and features.