1.Tolerance to Low Temperature of Transgenic Aloe Plants with TaDREB Gene from Wheat
Hua ZHAO ; Jin ZHAO ; Yinmao DONG ; Congfen HE ; Qin ZHONG
China Biotechnology 2006;0(09):-
Plant expression cassette for TaDREB from wheat was constructed into plasmid pBIR1.Aloe stems were used as explants for the transformation mediated by Agrobaterium.Infected tissues were selected using G418 to generate transformants.In total,58 resistant plantlets to the antibiotics were obtained from the infected explants.The designed primers according to the selective gene npt II and the target gene TaDREB were used to analyze all of the G418 resistant plantlets.PCR results demonstrated that TaDREB were successful transferred into aloe genomic with the transformation efficiency of 0.5%.The transgenic aloe plants were treated under 4℃ for two weeks and then at-20℃ for 30min.The treatment showed that the leaves of negative plants appeared severe evidence of freeze injury with brown,withered and translucent,while the positive plants appeared good growing condition.The activities of enzymes such as peroxidase(POD)and superoxide dismutase(SOD)of transgenic plants which were stressed for 14 days under low temperature were analyzed.The results indicated that the trend of SOD and POD activities in transgenic plants was down-up-up-up,and that in non-transgenic plants was down-up-down-down.The average value of relative electrical conductivity in the positive plants was 0.456 which was lower than 0.685 in the negative plants.It is supposed that transformation of the kind of gene could improve the resistant ability of aloe to low temperature.
2.Construction of traditional Chinese medicine resources information spatial database.
Yu-yang ZHAO ; Cheng-zhong SUN ; Ze-dong YANG
China Journal of Chinese Materia Medica 2015;40(6):1219-1222
The informatization of traditional Chinese medicine resources is the basis of modern medicine. With a spatial attribute traditional Chinese medicine resources could be carried out for in-depth spatial analysis, data mining and traditional Chinese medicine resources regional industrial layout. In this paper, we took the data of Glycyrrhiza uralensis in the third national Chinese medicine resources survey as the experimental data, described the principles and structure of traditional Chinese medicine resources spatial information database. We also described the establishment of analysis model with the help of this spatial database.
Data Mining
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Databases, Factual
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Medicine, Chinese Traditional
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Plants, Medicinal
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chemistry
3.RFA combined with TACE and ethanol injection for unresectable hepatic carcinoma
Lihui ZHANG ; Zhong CUI ; Qingtao ZHAO ; Dong DU
Chinese Journal of General Surgery 2001;0(09):-
Objective To explore the effect of radiofrequency ablation (RFA) combined with transcatherter arterial chemo-embolization (TACE) and percaulaneous ethanol injection (PEI) for unresectable hepatic malignancies. Methods The clinical data of 41 cases of unresectable liver cancer(URLC)treated by RFA,TACE and PEI were analysed retrospectively. Results There were 30 cases of primary hepatic cancer(PHT) and 11 cases of secondary hepatic cancer in this series.Ultrasound,CT and MRI showed the tumors shrinking or stable in 26 of the 41 patients postoperatively.AFP decreased to normal in 12 cases of 16 AFP positive PHT patients after operation. No severe complication was seen in the series. Conclusions RFA combined with TACE and PEI is a safe, well-tolerated and effective method for unresectable hepatic carcinoma, and may improve the treatment efficacy of URLC.
4.Relationship between paraquat tissue content and organ injury in paraquat poisoning rats.
Bo ZHAO ; Xiang-dong JIA ; Zhong-chen ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(3):220-223
Animals
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Brain
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pathology
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Hydroxyproline
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metabolism
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Kidney
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pathology
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Liver
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pathology
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Lung
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metabolism
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pathology
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Male
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Paraquat
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metabolism
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poisoning
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Rats
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Rats, Wistar
5.Expression of Death Receptor 4 and Death Receptor 5 in Human Craniopharyngioma
pei-dong, LI ; zhong-wei, ZHAO ; xin-jun, WANG ; dong-ling, GAO ; yun-han, ZHANG
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To investigate the expression and significance of death receptor 4(DR4) and DR5 in human craniopharyngioma.Methods The expression of DR4 and DR5 was determined by immunohistochemistry and in situ hybridization in 28 samples of craniopharyngioma and 25 samples of normal brain tissue.Results With low expression in partial normal brain tissue,DR was expressed highly in all of the craniopharyngioma samples.High DR expression in craniopharyngioma tissue differed from low DR expression in normal brain tissue(P0.05).Conclusions High DR expression is prevalent in craniopharyngioma tissue.This may contribute to the apoptosis-induced therapy of craniopharyngioma.The control of DR expression lays in protein level.This may contribute to the selective induced-apoptosis of tumor necrosis factor-related apoptosis-induced ligand.
6.A case of laparoscopic cystogastrostomy for pancreatic abscess.
Hua FAN ; Dong ZHANG ; Xin ZHAO ; Fei PAN ; Zhong-kui JIN
Chinese Medical Journal 2012;125(4):717-718
Abscess
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surgery
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Humans
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Laparoscopy
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Male
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Middle Aged
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Pancreatic Diseases
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surgery
8.Expression and Clinical Significance of bcl-X_L mRNA and Its Protein in Childhood Medulloblastoma
xin-jun, WANG ; zhong-wei, ZHAO ; ji-xin, SHOU ; pei-dong, LI ; qiao, SHAN ; quan, LIU
Journal of Applied Clinical Pediatrics 2006;0(23):-
Objective To study the expression level and clinical significance of bcl-XL gene in childhood medulloblastoma.Methods The expression of Bcl-XL protein and bcl-XL mRNA were determined by immunohistochemical staining and in situ hybridization in 41 samples of medulloblastoma tissues,as well as 20 normal brain tissues.Results The positive rate of Bcl-XL protein(90.2%) and bclXL mRNA(95.1%) in medulloblastoma group were significantly higher than those in normal human brain tissues(all P
9.Clinical application of double support plate via posteromedial approach in the treatment tibial plateau fracture of posterior column
Shengli DONG ; Dianying ZHANG ; Xiaotao ZHAO ; Kai YU ; Jianpeng LI ; Zhong YANG
Tianjin Medical Journal 2015;(10):1179-1182
Objective To observe the clinical effects of double support plate via posteromedial approach in the treat?ment of tibial plateau fracture of posterior column. Methods A total of 21 cases of tibial plateau fracture in closed posterior column with an average age of 34.6 (range, 21 to 56) years old were treated using double support plate through posteromedial approach from January in 2010 to January in 2013(16 males and 5 females). Among all, 4 cases were combined with lateral column fracture and 17 cases were three column fracture. Wound healings were observed after operation. X-ray examina?tions were performed at 2 weeks, 3 months, 4 months, 6 months and 12 months after operation as well as at last follow up. The averaged healing time was calculated. Changes of tibial plateau angle and posterior slope angle were compared between time points at 2 weeks after operation and at last follow up. Rasmussen knee score criteria was employed to assess knee joint recovery. Results The mean follow-up was (18.2 ± 1.8) months. No infection was reported. The average fracture healing time was 3.8(3.8±0.6)months. There was no significant difference between tibia plateau angle and posterior slope angle be?tween time points at 2 weeks after operation and at last follow-up (P<0.05). According to Rasmussen knee score criteria at last follow-up, 13 cases were graded as excellent, 6 cases as good, and 2 case as fair, with an excellent and good rate of 90.5%. Conclusion With posteromedial approach, the fractures of medial column, posteromedial and posterolateral of pos?terial column can be exposed well. Using double support plates to fix posteromedial and posterolateral of posterior column fracture can effectively prevent loss of reduction and the function of knee recovered well postoperatively.
10.Effect of high-density lipoprotein on interleukin-8 secretion in 3T3-L1 adipocytes
Qiaoqing ZHONG ; Shuiping ZHAO ; Xing WANG ; Bilian YU ; Jing DONG ; Xiangzhu XIE ; Zhihong WU
Chinese Journal of Endocrinology and Metabolism 2010;26(10):888-890
3T3-L1 adipocytes were cultured with various concentrations of high-density lipoprotein ( HDL, 0, 10, 50, and 100 μg/ml ) for 16 h and with lipopolysaccharide ( LPS, 100 ng/ml ) for another 6 h. Interleukin-8 in the medium was determined by ELISA, and PPAR-γ mRNA expression by reverse transacription polymerase chain reaction (RT-PCR). Interleukin-8 levels were increased in LPS-treated cells ( P<0.05 ), but decreased in HDL-treated cells in the dose-dependent manner. PPARγ mRNA expressions were increased in HDL-treated groups than those treated only with LPS. These results suggested HDL may decrease interleukin-8 secretion via up-regulating PPARγ expression in adipocytes.