Central Nervous System Diseases ; complications ; Humans ; Pulmonary Edema ; etiology

Objective:To investigate the protective effects of acupuncture pretreatment on ischemic myocardium,the protective mechanism of acupuncture pretreatment on ischemic myocardium was explored by observing the cardiac muscle cell apoptosis and the expression of HSP70 mRNA of ischemia-reperfusion injury rats treated with acupuncture pretreatment.Methods:Sixty-four Wistar rats were randomly divided into eight groups:control group,sham surgery group,ischemia-repertusion group,ischemia pretreatment group,manual acupuncture pretreatment group(once a day),electroacupuncture pretreatment group(once a day),manual acupuncture pretreatment group(twice a day),and electroacupuncture pretreatment group(twice a day).The reperfusion model of rat myocardial ischemia was made.Expression of HSP70 mRNA was assayed by in situ hyrbridization,and cell apoptosis by TUNEL.Results:Compared with those in the control group and the sham surgery group,the apoptosis and the expression of HSP70 mRNA were increased in the ischemia-reperfusion group.Compared with those in the ischemia-reperfusion group,the cardiac muscle cell apoptosis was decreased and the HSP70 mRNA was increased in the rats treated with acupuncture pretreatment;meanwhile,acupuncture pretreatment twice a day had stronger effects than acupuncture pretreatment once a day and ischemia pretreatment.Conclusion:Acupuncture pretreatment can inhibit the cardiac muscle cell apoptosis,and up-regulate the expression of HSP70 mRNA in ischemia-reperfusion rats.Acupuncture pretreatment twice a day has stronger effects than pretreatment once a day.

Animal models of endometriosis with traditional Chinese medicine (TCM) symptoms, not only provided a useful experimental method for TCM syndrome differentiation of endometriosis, also verified the pathogenesis of TCM of endometriosis, which offered the theoretical support for the clinic. This article discussed the establishment, result examination, existing limit, and solutions of animal models of endometriosis with TCM symptoms.

Objective To observe effects of theYubaisang-Zhikefangdecoction on chronic bronchitis.MethodsA total of 128 patients were randomly divided into two groups. The control group was given the conventional therapy, and the treatment group was added theYubaisang-Zhikefang decoction based on the conventional therapy.ResultsThe total effective rate of treatment group was significantly better than the control group (92.58%vs. 79.37%;χ2=4.434,P=0.035). The treatment group significantly improved the symptoms of time of cough disappearance(3.28 ± 1.21 d vs. 5.77 ± 1.45 d;t=10.562,P<0.05), time of asthma stop (8.02 ± 3.29d vs. 10.83 ± 7.17 d;t=2.864,P<0.05), time of sputum disappearance (3.27 ± 1.18d vs. 5.18 ± 1.16 d;t=9.232,P<0.05), time of moist sounds disappearance(4.53 ± 1.27 vs. 7.96 ± 1.45 d;t=14.249, P<0.05). And the treatment group significantly improved the results of blood tests and lung imaging, like the WBC (5.9×109/L ± 2.7×109/L vs. 8.1×109/L ± 2.6×109/L;t=4.693,P<0.05), N% (63.3% ± 6.1% vs. 69.2% ± 6.5%;t=-4.68,P<0.05), CRP (14.5 ± 5.9 mg/L vs. 18.4 ± 4.8 mg/L;t=5.297,P<0.05).ConclusionThe integrated treatment ofYubaisang-Zhikefangdecoction plus convertional the rapy showed good effects on the patients with chronic bronchitis.

OBJECTIVE: To study the water-soluble constituents of Clerodendranthus spicatus. METHODS: Various column chromatographic techniques were used to isolate the constituents, and their structures were identified by comparing their spectral data with those in literature. RESULTS: Fifteen compounds were isolated and elucidated as caffeic acid(1), caffeic acid methyl ester(2), caffeic acid ethyl ester(3), danshensu(4), ethyl 3, 4-dihydroxyphenyllactate(5), rosmarinic acid(6), methyl rosmarinate(7), ethyl rosmarinate(8), isorinic acid(9), dihydroconiferyl alcohol(10), dihydrosinapyl alcohol(11), salvianolic acid C(12), salvian-olic acid H(13), 3′-O-(8″-Z-caffeoyl) rosmarinic acid methyl ester(14) and baicalein(15). CONCLUSION: Compounds 2, 4-5 and 9-15 are obtained from this species for the first time.

C-C motif chemokine ligand 2 (CCL2) and its receptor CCR2 are closely related to tumorigenesis and tumor progression. The CCL2/CCR2 signaling axis promotes tumor progression through multiple mechanisms: CCL2 binds to CCR2 on the surface of tumor cells, and thus promotes tumor growth/survival and metastasis; more importantly, CCL2 recruits a variety of immunosuppressive cells to aggregate in the tumor microenvironment, and inhibits the function and activity of immune cells, promoting tumor progression. The article reviews the CCL2/CCR2 signaling axis and its role in tumors and tumor microenvironment, with particular focus on the advances in clinical research on drugs targeting CCL2/CCR2 signaling axis, in order to gain an in-depth and overall understanding of the mechanism of action of CCL2/CCR2 axis in tumor progression and develop more effective anti-tumor immunotherapeutic agents.

Objective To investigate the imaging features of dysplasia epiphysealis hemimelica (DEH).Methods Thirteen cases of DEH confirmed by pathology (between 1.5 to 19.0 years old)underwent X-ray scan.Additionally,CT scan was performed in one patient,MR scan was performed in one,and CT and MR scan were both performed in one.Results The lesions all occurred in lower limb.There were 32 lesions,and multiple lesions were detected in 8 cases.Lesions involved holo-epiphyses in 6 cases,Twenty-three lesions were in the medial limbs,3 were in the lateral limb.With Azouz classification,the limited form was identified in 5 cases,while the classic form was identified in 8 cases.Among 13 cases,there were 4 cases with ankle eversion,3 cases with the knock knee,2 cases with the ankle inversion,and 2 cases with the strephexopodia.The metaphysis of 8 cases were widened and deformed,and the provisional zone of calcification of 1case disappeared partly and the epiphysis closed prematurely.Conclusions One or multiple eccentric enlarged ossification center of unilateral or bilateral limb is the imaging feature of DEH.There is sporadic calcification or ossification on the prophase.The calcification or ossification can fuse each other to form agglomerate at the advanced stage.The bone can deform and shift when compressed by the mass,and bone defect can even occur.

Objective To evaluate the relationship between ambulatory arterial stiffness index (AASI) and intracranial and extracranial arterial stenosis in 146 patients with ischemic cerebrovascular disease. Methods Degree of intracranial and extracranial arterial stenosis in 146 patients with ischemic cerebrovascular disease was assessed by CT angiography (CTA). Then all patients were divided into 4 groups according to the numbers of branches with stenosis: no stenosis group, 1 branch group, 2 branches group, and 3 and more than 3 branches group. Dynamic blood pressure was monitored to calculate the AASI. Results There was no significant difference of AASI among the extracranial arterial stenosis groups (P=0.614). AASI was positively correlated with the degree of intracranial artery stenosis (r=0.743, P<0.05), and with 3 and more intracranial artery branches stenosis (r=0.797, P<0.05). Conclusion AASI is closely correlated with the degree and numbers of branches of intracranial artery stenosis.

Objective To observe the effects of hot compress on oxaliplatin-induced neurotoxicity.Methods One hundred and twenty patients undergoing chemotherapy with oxaliplatin enrolled in this study were randomly assigned to the control group and expenment group.The control group was given routine health education and the experiment group was treated with hot compress.We compared the incidence of nerotoxocity after 2 and 4 treatment courses.Resulsts The incidence of neurotoxiticity was 75.0%in the experiment group and 91.7%in the control group after 2 treatment courses(P<0.01).The incidences were 86.7%in the experiment group and 98.3%in the control group after 4 treatment courses(P<0.001).Conclusion Hot compress can effectively reduce the incidence of neurotoxicity induced by chemotherapy with oxaliplatin.

BACKGROUND: Following peripheral nerve transplantation, the time phases and law of growth associated protein 43 (GAP-43) expression in sensory neuron soma of the corresponding dorsal root ganglia remain unclear.OBJECTIVE: To investigate the expression of GAP-43 at the corresponding dorsal root ganglia following sciatic nerve transplantation in rats.METHODS: Adult male Wistar rats were randomly divided into 2 groups: control group (sham-operated group) and experimental group (nerve transplantation group). The rats were killed at 3 days, 1, 2, 4, 6, and 8 weeks after operation and the dorsal root ganglia U-s of every rat was taken for observation. The expression of GAP-43 mRNA and protein in the corresponding segments of spinal cord were detected by RT-PCR and Western-blot techniques.RESULTS AND CONCLUSION: GAP-43 mRNA showed a low level of expression in the control group, which had no obvious changes in different phases. In the experimental group, GAP-43 mRNA expression was observed remarkably in the dorsal root ganglia at the first week, reached a peak at the second week and gradually descended from the sixth week. The GAP-43 protein expression showed the same laws as that of GAP-43 mRNA. The results demonstrated that there exists damage reactivity change in the regeneration ability of neurons.