1.Successful pregnancy after amniotic fluid embolism.
Zhao-yi FENG ; Chun-yan SHI ; Hui-xia YANG ; Xue-lian GAO ; Yan-zhi JIN
Chinese Medical Journal 2013;126(14):2799-2799
2.Inhibitory effect of docetaxel on proliferation of human lens epithelial cells
Shao-ling, YI ; Bin, SHI ; Wan-wen, LI ; Li-jian, XU ; Chun-shun, ZHAO
Chinese Journal of Experimental Ophthalmology 2011;29(1):32-37
Background Some drugs with inhibitory effect on the proliferation of lens epithelial cells have a limiting application in clinic because of their adverse response.To screen the effective and less side-effect drug for supressing LECs growth is very inportant for the prevention and treatment of after cataract.Objective This study was to explore the effects of docetaxel on LECs growth and compare its role with epirubicin hydrochloride,pirarubicin hydrochloTide and rahitrexed.Methotis Immortalized human LECs line (SRA01/04) were cultured and passaged.Different concentrations of docetaxel,epirubicin hydrochloride,pirarubicin hydrochloride and rahitrexed were added into the medium respectively for 24.48 and 72 hours.The proliferation of LECs was detect by M1Yr.Flow cytometry analysis Was used to analyze the influence of different concentrations of docetaxel on cellular cycle at 48 hours after addition of docetaxel,and Annexin V-FITC/PI marking method was used to assesse the apoptosis of LECs under the action of docetaxel.Expression of bcl-2 protein in LECs Was evaluated by Westeru blot. Result The growth rate of LECs Wag 100%in 8-519 pmol/L doeetaxel groups with the normal cell shape.Majority of abnormal cells and low growth rate were found in 66 nmoVL docetaxel group at 48 and 72 hours.The IC50 of docetaxel was lowest in 48 and 72 hours in docetaxel group in comparison to epirubicin hydrochloride and pirarubicin hydrochloride. However,no evident inhibition on LECs growth in 23.22-523.56 μmol/L of raltitrexed.At 48 hours,the percentage of LECs in G2/M phase increased as the asccnte of concentration of docetaxel,showing a significant difference among 4 groups(F=2633.05,P<0.01).The percentage of early apoptotic cells increased to 22.4%(χ2=20.00,P<0.01) and 27.9%(χ2=42.68,P<0.01)from normal control 3.1% at 48 hours after LECs exposed to 8.3 nmol/L and 266 nmol/L docetaxe.The expression of bcl-2 protein in LECs was obviously weakened after addition of docetaxel,especially 8.3 nmol/L docetaxel group. Conclusion Docetaxel,epirubicin hydrochloride and pirarubicin hydrochloride can inhibit the proliferation of human LECs in vitro.But there is no supression on LECs growth inraltitrexed.Docetaxel is proved to have a strongly arrested effect on the proliferation of LECs in comparison with epirubicin hydrochloride and pirarubicin hydrochloride and play its role at concentration-and time-dependent manner.
3.Implementation of eye movement tracking system based on camshift algorithm.
Chun-Rui HUANG ; Xue-Quan LV ; Ji ZHAO ; Qiu-Shi REN ; Xin-Yu CHAI
Chinese Journal of Medical Instrumentation 2009;33(4):239-242
In this article, the implementation of eye movement tracking system includes three procedures: hardware acquisition, data extraction and overall analysis. The system is based on Camshift algorithm with an eye tracking module added, developed on VC++ 6.0. The system can track the eye movement effectively in simulated phosphene evaluation experiment based on prosthetic vision.
Algorithms
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Analysis of Variance
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Eye Movements
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physiology
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Prosthesis Design
5.Primary Hodgkin lymphoma of the intestinal tract: report of a case.
Wei-Dong SHI ; Chun-Nian HE ; Huan-Fen ZHAO ; Ping LI ; Chen CHEN ; Xiu-Zhi ZHANG ; Shu-Yan ZHANG
Chinese Journal of Pathology 2008;37(7):493-494
Aged
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Female
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Hodgkin Disease
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diagnosis
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pathology
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Humans
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Reed-Sternberg Cells
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pathology
6.Goblet cell carcinoid of appendix: report of two cases.
Xue-dong ZHANG ; Chun-nian HE ; Jin-ping ZHAI ; Huan-fen ZHAO ; Chen CHEN ; Wei-dong SHI
Chinese Journal of Pathology 2006;35(2):126-127
Adenocarcinoma, Mucinous
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pathology
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Aged
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Appendectomy
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methods
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Appendiceal Neoplasms
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pathology
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surgery
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Appendicitis
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pathology
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Appendix
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pathology
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Carcinoid Tumor
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pathology
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surgery
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
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Male
7.Influence of solvent and drug preparation time on Shuanghuanglian injections induce pseudo-allergic reaction.
Yan YI ; Ai-hua LIANG ; Chun-ying LI ; Yu-shi ZHANG ; Yong ZHAO ; Jia-yin HAN ; Yu-ting LU
China Journal of Chinese Materia Medica 2015;40(14):2723-2726
Choosing the right solvent and timely use is the basis of rational drug use and the most direct and efficient way to improve the safety of traditional Chinese medicine injections. This study aimed to evaluate the influence of solvent and drug preparation time on Shuanghuanglian injection inducing pseudo-allergic reactions with mouse mode. The two tests were carried out: (1) Comparative experiment between different solvent: Shuanghuanglian injection preparation to the appropriate concentration with 0.9% sodium chloride injection and 5% dextrose injection, mixed with Evans blue, at one time intravenous injected into mice, 30 minutes later, the mouse ears vascular permeability were observed and compared. (2) Comparative experiment among different preparation time: placed 10 min, 2.5 h, 6 h and 24 h after Shuanghuanglian injection were prepared and then to detect the pseudo-allergic reactions in mice using the same methods as in (1). The results showed that there was no significant difference in the pseudo-allergic reactions in mice which induced by the same dose of Shuanghuanglian injection, respectively with 0.9% sodium chloride injection and 5% dextrose injection preparation, and with the extension of preparation time, the degree of pseudo-allergic reactions of Shuanghuanglian injection was gradually severe.
Animals
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Drug Compounding
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Drug Hypersensitivity
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etiology
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Drugs, Chinese Herbal
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adverse effects
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Injections
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Male
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Mice
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Mice, Inbred ICR
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Solvents
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Time Factors
8.Evaluation and study of pseudo-allergic reactions of three kinds of traditional Chinese medicine injections and its mechanisms.
Yan YI ; Chun-ying LI ; Yu-shi ZHANG ; Yong ZHAO ; Jia-yin HAN ; Yu-ting LU ; Ai-hua LIANG
China Journal of Chinese Materia Medica 2015;40(14):2711-2716
To evaluated the pseudo-allergic reactions of cordate houttuynia, pulse-activating and Qingkailing injection in mice, the ICR mouse were divided into different test groups, then were intravenously injected with three traditional Chinese medicine injections, positive control compound 48/80 and physiological saline as normal control. All test substances were mixed with 0.4% Evans blue. The reaction and vascular permeability of the ears were observed and measured 30 min after injection. At the same time, the mechanisms of the traditional Chinese injections' pseudo-allergic reactions was studyed. ICR mice were injected with the test substances as above without EB, blood in a part of mice were collected after 5 min, and the level of histamine in the plasma were measured. Blood in the other part of mice were collected after 30 min, and the level of VEGF, TNF-α and IL-10 in the serum were measured. The reasults showed that except the cordate houttuynia injection, pulse-activating injection in 1. 5 times clinical concentration and Qingkailing injection in 3.3 times clinical concentration caused mild pseudo-allergic reactions mainly for vascular permeability, no pseudo-allergic reactions occurred when the concentration of the two injections was below the concentration metioned above; 5 minutes after intravenous injection of the three TCM injections into ICR mice with the highest dose, the levels of histamine in plasma of pulse-activating injection and Qingkailing injection groups were increased significantly, 30 minutes later, the levels of VEGF, TNF-α and IL-10 in the serum of all groups were no significant change compared to normal group. The mouse of pulse-activating and Qingkailing injection groups showed inflammatory changes in ear and lung tissues. It can be conluded that when the dose or concentration increased to a certain extent, pulse-activating and Qingkailing injection could induce pseudo-allergic reactions on ICR mice, the increased histamine realease maybe the main reason for pseudo-allergic reactions of the two traditional Chinese medicine injections. In addition the author preliminary thought that inflammatory mechanisms leading to hyperpermeabilities were the main reason of the traditional Chinese medicine injection's pseudo-allergic reaction.
Animals
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Drug Hypersensitivity
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etiology
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Humans
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Injections
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Interleukin-10
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blood
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Male
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Medicine, Chinese Traditional
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adverse effects
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Mice
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Mice, Inbred ICR
9.Effects of Shibao Powder on promoting the expression of b-FGF and TGF-beta 1, in the repair of soft tissue injuries.
Dao-zhou ZHAO ; Wen-yu SHI ; Peng-gui ZHANG ; Chun-lei ZHANG
China Journal of Orthopaedics and Traumatology 2008;21(9):667-668
OBJECTIVETo study the effects of Shibao Powder on the expression of b-FGF and TGF-beta 1 in granulation tissues of rabbit models with soft tissue injuries, so as to explore mechanism of external Chinese drugs for repairing of soft tissues in molecular levels.
METHODSThe rabbit models were established by classical method of full-thickness skin wounds. After 8% sodium sulfide was used and routine disinfection completed, intra-peritoneal anaesthesia was adopted. The skin was cut to expose gastrocnemius muscle, and imcomplete sharp dissection was made near the tendon insertion. The length of the incision was 0.8 cm and the width was 0.4 cm. The rabbits in the experimental group were treated with spread of Shibao Powder at the wound; the rabbits in clean group were treated with routine dressing changes and disinfection; and the rabbits in the control group were treated with dressing changes only. The granulation tissues in different stages were collected and observed with high power microscope. The expression of Transforming Growth Factor-beta 1 (TGF-beta 1) protein and b-FGF protein in wound tissues were detected using StreptA-ridin-Biotin-Complex (SABC) method.
RESULTSThe results showed that the expression of b-FGF protein had significant differences among 3 groups at the 6th day after trauma (P<0.05). The TGF-beta 1 protein expression also had significant differences among 3 groups at the 10th and 14th days after trauma (P<0.05). At the same time, the growth states of granulation tissues had difference among 3 groups at the same trauma stage, and within the same group among different trauma stages.
CONCLUSIONThis experimental study shows that Shibao Powder is effective to promote the repair of soft tissues after trauma by stimulating production of endogenous growth factor from cells in wound.
Animals ; Disease Models, Animal ; Fibroblast Growth Factors ; metabolism ; Gene Expression Regulation ; drug effects ; Granulation Tissue ; drug effects ; metabolism ; pathology ; Male ; Medicine, Chinese Traditional ; Powders ; Rabbits ; Soft Tissue Injuries ; drug therapy ; metabolism ; pathology ; Transforming Growth Factor beta1 ; metabolism ; Wound Healing ; drug effects
10.Comparison of therapeutic effects of acupuncture-cupping plus acupoint catgut embedding and electroacupuncture on simple obesity of stomach and intestine excess-heat type.
Yin SHI ; Lin-shan ZHANG ; Chen ZHAO ; Chun-qing HE
Chinese Acupuncture & Moxibustion 2006;26(8):547-550
OBJECTIVETo search for a therapy of increasing clinical therapeutic effect on simple obesity with stomach and intestine excess-heat.
METHODSEighty-two cases of simple obesity with stomach and intestine excess-heat were randomly divided into group A (n=40) and group B (n=42). They were treated with electroacupuncture (EA), movable cupping plus acupoint catgut embedding, and simple EA, respectively. EA at Zhongwan (CV 12), Xiawan (CV 10) and Qihai (CV 6) were given in the two groups. And in the group A, acupoint catgut embedding and movable cupping at the Channels CV, GV, SP, ST and UB were added. The therapeutic effect, main symptoms, body mass index (BMI), waist circumference (WC), hip circumference (HC), and WHR were investigated.
RESULTSThe total effective rate was 90.0% in the group A, significantly better than 78.6% in the group B (P< 0. 01), with significant differences in decrease of body weight, BMI, WC, HC and main symptoms between the two groups (P<0.05, P<0.01).
CONCLUSIONAcupuncture, cupping plus acupoint catgut embedding therapy can increase therapeutic effect on simple obesity of stomach and intestine excess-heat type, and it is a better method for treatment of this disease.
Acupuncture Points ; Acupuncture Therapy ; Catgut ; Electroacupuncture ; Hot Temperature ; Humans ; Intestines ; Obesity ; therapy ; Stomach