BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix.
OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels.
METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28.
RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.

BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.

Objective To study the effect of atlantoaxial pedicle and lateral mass screw in treat-ment of atlantoaxial instability. Methods A retrospective analysis was done on 11 eases of aflantoaxi-al instability treated with atlantoaxial pedicle and lateralmass screw from June 2006 to December 2007. Results The postoperative radiographs verified good position of all screws, with satisfactory atlantoaxial reduction. Follow-up for 3-21 months (average 12 months) showed no spinal cord and vertebral artery in-jury or interfixation failure. Conclusion Atlantoaxial pediele and lateral mass screw technique is a convenient method with solid fixation for treatment of atlantoaxial instability.

Prevention of infection by the human papillomavirus (HPV) has become a hot research topic since the relationship between the HPV and cervical cancer was confirmed. Persistent infection with HPV and early expression of proteins has an important role in the pathogenesis of cervical cancer. Vaccines that protect against four high-risk types of HPV (-6, -11, -16, -18) have been used worldwide. A bivalent vaccine (HPV-16 and -18) developed by Walvax is in clinical trials. This study reviews progress in ascertainment of the structure and function of the HPV genome, the molecular mechanism of carcinogenesis, and vaccines based on virus-like particles.
Animals ; Carcinogenesis ; Female ; Humans ; Papillomaviridae ; genetics ; immunology ; metabolism ; Papillomavirus Infections ; pathology ; prevention & control ; virology ; Papillomavirus Vaccines ; genetics ; immunology ; Uterine Cervical Neoplasms ; pathology ; prevention & control ; virology ; Viral Proteins ; genetics ; immunology ; metabolism

Ear Diseases ; congenital ; surgery ; therapy ; Ear, External ; abnormalities ; Ear, Middle ; abnormalities ; Humans

Objective To observe the effects of comprehensive psychological interventions in the treatment of abnormal blinking in children.Methods One hundred and sixty-three children diagnosed with abnormal blinking were randomly assigned into a basic treatment group or a comprehensive psychological intervention (CPI) group.Both groups received etiology-based therapy,while the CPI group received CPI in addition.After follow-up for six months,the therapeutic effectiveness and recurrence were evaluated.Results Abnormal blinking was significantly reduced in both groups after treatment,but the CPI group showed significantly better effectiveness than the group which received only basic treatment.Furthermore,the CPI group showed significantly less recurrence at 3-and 6-month follow-up.Conclusion CPI is more effective than basic treatment in treating abnormal blinking among children and results in less recurrence.

BACKGROUND: Currently, a variety of new dressings have been on the market, which are diversified and exhibit multifunctional trends. However, ideal wound dressings are still in exploration.OBJECTIVE: To introduce the basic physiological function and polymer advantage of dextran as well as its effects to promote wound healing in combination with other macromolecule materials, in order to impel the development of dextran as a new medical dressing .METHODS: A computer-based search of CNKI, PubMed, WanFang, VIP databases was performed to retrieve reviews or research articles addressing dextran and medical dressings published from January 2000 to December 2016. The keywords were dressing, dextran, wound healing in Chinese and English, respectively.Finally, 31 articles were included in result analysis.RESULTS AND CONCLUSION: Dextran has the physiological functions of promoting wound healing and immune function. These physiological functions are the basis of dextran as a medical dressing. Additionally, dextran has some polymer advantages, such as water absorption, biodegradability and non-toxicity. It is noteworthy that dextran can be combined with macromolecules to produce new polymer materials that can promote wound healing in animal experiments. Taken together, dextran, as a medical dressing, has a broad clinical prospect in wound healing.

Objective To explore the features of clinical and progression of multiple system atrophy(MSA).Methods The clinic data of 28 subjects diagnosed as probable MSA according to Gilman diagnostic criteria were studied retrospectively.Three aspects of activities of daily living(ADL)(aid-requiring walking,wheelchair-bound state and bedridden state)were used to assess the progression of disease.Kaplan-Meier analysis was also used to estimate the difference between subgroups.Results Three systems were involved in 26 cases(92.9%)and autonomic functional disturbance was common.The calculated median time from onset to evolution to MSA was mean 2 years.The median times from onset to aid-requiring walking,wheelchair requirement and bedridden state were 3,5 and 7 years,respectively.The patients impaired both of motor and autonomic systems progressed fastly within 3 years from onset of the disease(P

OBJECTIVE To evaluate the application of antibiotics and its trend for reference to clinical utilization.METHODS The data about antibiotics used from 2005 to 2007 were collected and analyzed.RESULTS The consumption of antibiotics was fallen year by year.The rate of utilization was 67%.The rate of drug combination was 45% and the rate of antibiotic resistance test was 20%.CONCLUSIONS There are still a lot of problems in antibiotics usage of our hospital.It is necessary to strengthen the guidance and supervision.

Objective The purpose of this study was to determine and compare the hemodynamic effects of desflurane and enflurane in children.Methods Twenty-eight ASA Ⅰ-Ⅱ patients, aged 1-5 years were randomly assigned to receive either desflurane (group D) or enflurane (group E) for maintenance of anesthesia.Cardiac output was continuously monitored by transesophageal Doppler (TECO1).The stroke volume index (SVI), cardiac index (CI),heart rate (HR) and blood pressure(SBP,DBP,MAP) were determined before and during inhalation of desflurane and enflurane at 0.5,1.0 and 1.5 MAC.Results At 0.5 MAC of both agents SVI, CI and HR remained unchanged, but SBP, DBP and MAP were slightly reduced as compared to the baseline values.At 1.0 MAC of desflurane CI was increased and was higher than that in the group E. SVI and HR were significantly higher in group D than those in group E at 1.0 MAC.SBP, MAP and DBP were further reduced and the magnitude of reduction was similar between both groups.At 1.5 MAC CI and SVI were unchanged in group D but greatly reduced in group E.HR increased significantly in group D but remained unchanged in group E.There was further decrease in BP in both groups but SBP and MAP decreased more markedly in group E than in group D .Conclusions Desflurane causes less hemodynamic changes in children of 1-5 years than enflurane.