Objective To study the variable number tandem repeats (VNTR) polymorphism in intron 4 of the endothelial nitric-oxide synthase (eNOS) gene and its relationship with plasma NO in pregnancy induced hypertension (PIH). Method The VNTR polymorphism in intron 4 of the eNOS genes of PIH and healthy control pregnant women were detected by PCR. The plasma level of NO~(3-)/NO~(2-) was measured by spectrophotometer, which was used as the index of NO synthesis.Data were analyzed according to the different type of genotypes and allelic gene frequency. Results The PIH group showed significant higher frequency of VNTR 4a allele and 4a/4b+4a/4a genotype than those of the control (16.00% vs 7.50%,P

OBJECTIVE:To study the pharmacokinetic features of Fagopyrum tataricum total flavonoids. METHODS:6 Beagle dogs were intragastrically given single dose of 80 mg F.tataricum total flavonoids (with content of quercetin at 1.2% and the content of eldrin at 24.7%). HPLC-UV spectrophotometry was adopted to determine the content of total quercetin in the hydrolyzed plasma samples. RESULTS:Taking the total quercetin as the index of the pharmacokinetic features of the F. tataricum total flavonoids in the Beagle dogs,the main pharmacokinetic parameters were as follows:AUC0→t:(9.92?1.49)?g?h?mL-1;AUC0→∞:(10.81?1.86)?g?h?mL-1;Cmax:(1.07?0.19)?g?mL-1. CONCLUSION:The pharmacokinetics of the F.tataricum total flavonoids in the Beagle dogs conforms to two-compartment model,and the concentration-time curves are characterized by multiple peak pharmacokinetics.

Objective To further study the blood supply mechanism of blocking flap and establish the standard of transferring the flap.Methods 7 groups of flap were made on 5 mini-Banna pigs.For each group,we had research flap and control flap on the right side and left side of each pig.After flap blocking operation,we detected the skin temperature of the flap on the 3rd,7th and 10th day.Results There was no statistic difference between each group before operation,but there was statistic difference after operation.Conclusion Temperature detecting may help choose the right time(32.5~33.5℃) for transferring the blocking flap.

The paper discusses the subject service of clinical medicine in the age of big data , including two aspects:clinical research and clinical medical treatment .It points out that the development and utilization of medical big data require for subject librarians and doc -tors with certain information literacy and proposes suggestion for enhancing the information literacy of subject librarians and doctors .

Objective To investigate the relationship between plasma low-density lipoprotein (LDL) levels and hematoma enlargement of early intracerebral hemorrhage,outcome at 3 months and death.Methods A total of 316 patients with primary intracerebral hemorrhage were included in the study.Their general information was documented.The patients underwent CT scans at 6 and 24 hours after onset.The lipids,blood glucose,blood pressure,and the National Institutes of Health Stroke Scale (NIHSS) scores were detected at the same time.The patients were followed up for 3 months and their modified Rankin scale (mRS) scores and the number of death were recorded.Results The decreased plasma LDL level (odds ratio [OR] 0.323,95％ confidence interval [CI] 0.128 - 0.819; P =0.017) and the increased systolic pressure (OR 1.015,95％ CI 1.000 -1.029; P =0.043) were independently associated with the early hematoma enlargement.The decreased plasma LDL level (OR 0.253,95％ CI 0.102-0.629; P=0.003) and the increased blood glucose (OR 1.458,95％ CI 1.257 - 1.693; P ＜ 0.001 ) were the independent risk factors for poor outcome at 3 months after onset.The decreased plasma LDL level (OR 0.211,95％ CI 0.075 - 0.597; P =0.003),the increased bloot glucose level (OR 1.406,95％ CI 1.212 - 1.632; P =0.001) and the increased blood glucose level (OR 1.026,95％ CI 1.009 - 1.043; P =0.002) were the independent risk factors for death within 3 months.Receiver operating characteristic curves showed that the LDL level ＜ 2.58 mmol/L was an independent predictor for hematoma enlargement (sensitivity 71.79％,specificity 64.71％,positive predictive value 40.00％,and negative predictive value 87.50％ ).Conclusion The decreased plasma LDL level was the independent predictor for early hematoma enlargement,poor outcome and death at 3 months in patients with primary intracerebral hemorrhage.

Objective: To propose essential thought for the disease pricing and to provide theoretical and methodological references for promoting disease pricing in China. Methods: Utilizing literature research method and theoretical deduction method are used to comb and analyze the theory of disease pricing theory. Results: On the basis of the research for disease cost calculation, key process of pricing like disease groups is proposed, benchmark disease and relative weight of diseases price and draws into degree of risk factors and technical difficulties. At the same time, it proposes the manipulation of cases outside the lines and pricing adjustment system. Conclusion: The research is scientific, prospective and innovative.

To explore the application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis, the DNA template was extracted by hydroxybenzene-chloroform from 44 maternal (7-41 weeks) plasma. The Fetus-derived Y sequence DYZ-1 gene (149bp) was chosen to be amplified by PCR. The fragment was identified in all the plasma of male bearing pregnant women with the diagnostic accordance rate being 100.00%. Two of the 22 female bearing pregnant women had false positive results. Among the 44 pregnant women, the diagnostic accordance rate was 88.89% at early pregnant stage, 100.00% at medium pregnant stage, and 96.55% at late stage respectively. The final accuracy of 95.45% was obtained in all cases. It was concluded that by means of hydroxybenzene-chloroform extraction the authors of this article promoted the concentration and purity of the DNA template, and diagnosed more accurately. The results showed that free fetal DNA in the maternal plasma could be regarded as the gene resource for noninvasive prenatal diagnosis.
DNA/*blood ; DNA Primers ; Fetus ; Maternal-Fetal Exchange ; Pregnancy/*blood ; Prenatal Diagnosis/*methods ; *Sex Determination (Genetics)

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Thalamic Diseases ; therapy

Because of the aggressive threaten of heat stroke and a lack of understanding of the mechanism of action, mammal animal models for experimental heat stroke were well developed. During the past 5 decades, anesthetized mouse, rat, rabbit, dog, baboon and monkey were used as animal model for experimental heat stroke. However, anesthetized mammals models have some limitations, such as neuroprotective effect of anesthetic agents, possible disturbance on injury and recovery of stroke animals by anesthetic agents, difficulty of discussing animal behavior before and after heat stroke, it was also difficult for the models to evaluate cognitive function of animal under hot environment. Considering humanitarian, only awaked and unrestrained mouse heat stroke model was accepted so far. Therefore, we also developed an awaked and unrestrained rat heat stroke model, and found it was helpful to evaluate drug effectiveness for animal behavior and cognitive function under hot environment.
Animals ; Cognition ; Disease Models, Animal ; Heat Stroke ; physiopathology ; Mice ; Rats

In this paper, we present a 3D virtual phantom design software, which was developed based on object-oriented programming methodology and dedicated to medical physics research. This software was named Magical Phan tom (MPhantom), which is composed of 3D visual builder module and virtual CT scanner. The users can conveniently construct any complex 3D phantom, and then export the phantom as DICOM 3.0 CT images. MPhantom is a user-friendly and powerful software for 3D phantom configuration, and has passed the real scene's application test. MPhantom will accelerate the Monte Carlo simulation for dose calculation in radiation therapy and X ray imaging reconstruction algorithm research.
Algorithms ; Humans ; Image Processing, Computer-Assisted ; Monte Carlo Method ; Phantoms, Imaging ; Software ; Software Design